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    • 7. 发明申请
    • IMPROVED PIG LIVER ESTERASES
    • 改善的肝脏食物
    • WO2010122175A1
    • 2010-10-28
    • PCT/EP2010/055564
    • 2010-04-26
    • DSM IP ASSETS B.V.KIETZMANN, MartinPICHLER, HaraldSCHWAB, HelmutEL-HELIEBI, AminBRAUN, AndreasWINKLER, Christine
    • KIETZMANN, MartinPICHLER, HaraldSCHWAB, HelmutEL-HELIEBI, AminBRAUN, AndreasWINKLER, Christine
    • C12N9/18
    • C12N9/18
    • The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in E.coli relative to the concentration of the fraction of the polypeptide without the mutation being present as an active and soluble protein in cleared lysate of the polypeptide without the one or more deletion or substitution expressed in E.coli under the same conditions. The invention also relates to nucleic acid encoding the polypeptides according to the invention, and the use of the polypeptides.
    • 本发明涉及具有酯酶活性的分离多肽,其包含SEQ ID NO:2,4,6,8,10,12或14中任一项所示的氨基酸序列或其同源物,其包含氨基酸取代或缺失的一个 或更多的氨基酸,如所述SEQ ID NO所示,并且导致突变体多肽的浓度增加,突变体多肽的分数作为活性和可溶性蛋白质存在于大肠杆菌中表达的突变多肽的清除裂解物中,相对于 没有突变的多肽的级分的浓度作为活性和可溶性蛋白质在多肽的澄清裂解物中存在,而在相同条件下没有在大肠杆菌中表达的一个或多个缺失或取代。 本发明还涉及编码根据本发明的多肽的核酸和多肽的用途。