专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20080311627A1 Compositions and Methods for Preventing Carry-Over Contamination in Nucleic Acid Amplification Reactions 审中-公开
标题翻译：防止核酸扩增反应中携带污染的组合物和方法
公开(公告)号：US20080311627A1
公开(公告)日：2008-12-18
申请号：US11663420
申请日：2005-06-17
申请人： Reimo Tetzner , Kurt Berlin , Juergen Distler
发明人： Reimo Tetzner , Kurt Berlin , Juergen Distler
IPC分类号： C12P19/34
CPC分类号： C12Q1/6848 , C12Q2527/101 , C12Q2521/301
摘要： Particular aspects provide methods for the specific amplification of template DNA in the presence of potentially contaminating PCR products from previous amplification experiments, but wherein the contaminating prior reaction amplificates (carry-over contaminants) are rendered non-amplifiable, based on use of particular contaminant degradation enzymes, and use of at least one primer that is fully complementary to any contaminating prior reaction amplificate nucleic acid, but contains a mismatch with the correspond sequence of the sample template nucleic acid. Additional aspects provide a method for the specific amplification of single-stranded sample template DNA in the presence of potentially double-stranded carry-over products. Further aspects provide methods comprising use of a template-dependent thermostable DNA polymerase enzyme suitable for incorporating ribonucleotides as well as deoxy-nucleotides to provide a chimeric amplificate. After digestion with an RNase, any contaminating prior chimeric amplificate, the RNase is inactivated. These aspects are surprisingly effective alternatives to the carry over protection system known as UNG system, and other art-recognized methods.
摘要翻译：特定方面提供了在来自先前扩增实验的潜在污染性PCR产物存在下对模板DNA进行特异性扩增的方法，但其中基于使用特定污染物降解使污染的先前反应扩增（携带污染物）变得不可扩增酶，以及使用与任何污染的先前反应扩增核酸完全互补的至少一个引物，但是含有与样品模板核酸的相应序列错配。另外的方面提供了在存在潜在双链携带产物的情况下单链样品模板DNA的特异性扩增的方法。另外的方面提供了包括使用适合于掺入核糖核苷酸的模板依赖性热稳定性DNA聚合酶以及脱氧核苷酸以提供嵌合扩增的方法。用RNase消化后，任何污染的先前的嵌合扩增，核糖核酸酶被灭活。这些方面对于被称为UNG系统的承载保护系统以及其他本领域公认的方法是惊人的有效的替代方案。

2. 发明申请

US20080064043A1 Method for detecting a methylation pattern 审中-公开
标题翻译：检测甲基化模式的方法
公开(公告)号：US20080064043A1
公开(公告)日：2008-03-13
申请号：US11881411
申请日：2007-07-26
申请人： Kurt Berlin , Juergen Distler , Reimo Tetzner
发明人： Kurt Berlin , Juergen Distler , Reimo Tetzner
IPC分类号： C12Q1/68 , C07H21/00 , G01N33/50
CPC分类号： C12Q1/6827 , Y10T436/143333 , C12Q2531/125 , C12Q2523/125 , C12Q2521/337
摘要： The present invention relates to a method for detecting the presence of one or more methylation patterns. It comprises the conversion of nucleic acids and a catalytic nucleic acid activity. Here, the conversion of the nucleic acid is characterized such that the 5-methylcytosine remains unchanged while the unmethylated cytosine is converted into uracil or another base, which can be distinguished from cytosine in its base-pairing behavior.
摘要翻译：本发明涉及一种用于检测一种或多种甲基化模式的存在的方法。它包括核酸的转化和催化核酸活性。这里，核酸的转化的特征在于5-甲基胞嘧啶保持不变，而未甲基化的胞嘧啶转化成尿嘧啶或其他碱，其可以与碱基配对行为中的胞嘧啶区分开。

3. 发明授权

US09695478B2 Methods and nucleic acids for the analyses of cellular proliferative disorders 有权
公开(公告)号：US09695478B2
公开(公告)日：2017-07-04
申请号：US12779856
申请日：2010-05-13
申请人： Catherine E. Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
发明人： Catherine E. Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

4. 发明申请

US20060286576A1 Methods and nucleic acids for the analyses of cellular proliferative disorders 有权
标题翻译：用于分析细胞增殖性疾病的方法和核酸
公开(公告)号：US20060286576A1
公开(公告)日：2006-12-21
申请号：US11405322
申请日：2006-04-17
申请人： Cathy Lofton-Day , Andrew Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
发明人： Cathy Lofton-Day , Andrew Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测或检测和鉴别肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之间的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

5. 发明授权

US08623599B2 Method for methylation analysis 有权
标题翻译：甲基化分析方法
公开(公告)号：US08623599B2
公开(公告)日：2014-01-07
申请号：US12451943
申请日：2008-06-06
申请人： Theo Devos , Cathy Lofton-Day , Andrew Sledziewski , Fabian Model , Michael Krouse , Jesse Ho , Matthias Schuster , Juergen Distler , Reimo Tetzner
发明人： Theo Devos , Cathy Lofton-Day , Andrew Sledziewski , Fabian Model , Michael Krouse , Jesse Ho , Matthias Schuster , Juergen Distler , Reimo Tetzner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q1/6806 , C12Q1/6827 , C12Q1/6844 , C12Q2523/125
摘要： Aspects of the invention relate to composition and methods for the providing of DNA for methylation analysis that is in particular suitable to be applied in reference laboratories. Further 5 aspects of the invention relate to composition and methods for the highly specific and sensitive methylation analysis of the Septin 9 gene also in particular suitable to be applied in reference laboratories.
摘要翻译：本发明的方面涉及提供用于甲基化分析的DNA的组合物和方法，其特别适用于参考实验室。本发明的另外5个方面涉及用于Septin 9基因的高度特异性和敏感的甲基化分析的组合物和方法，特别适用于参考实验室。

6. 发明授权

US07749702B2 Methods and nucleic acids for the analyses of cellular proliferative disorders 有权
标题翻译：用于分析细胞增殖性疾病的方法和核酸
公开(公告)号：US07749702B2
公开(公告)日：2010-07-06
申请号：US11405322
申请日：2006-04-17
申请人： Catherine E. Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
发明人： Catherine E. Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
IPC分类号： C12Q1/68 , C12Q1/44 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测或检测和鉴别肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之间的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

7. 发明申请

US20110171637A1 METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS 审中-公开
标题翻译：用于细胞增殖性疾病分析的方法和核酸
公开(公告)号：US20110171637A1
公开(公告)日：2011-07-14
申请号：US12374650
申请日：2007-07-23
申请人： Reimo Tetzner , Andrew Z. Sledziewski , Catherine Lofton-Day , Juergen Distler , Fabian Model
发明人： Reimo Tetzner , Andrew Z. Sledziewski , Catherine Lofton-Day , Juergen Distler , Fabian Model
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/04 , G01N30/00
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among prostate cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测或检测和鉴别前列腺细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之中的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

8. 发明申请

US20110009277A1 METHOD FOR METHYLATION ANALYSIS 有权
标题翻译：甲基化分析方法
公开(公告)号：US20110009277A1
公开(公告)日：2011-01-13
申请号：US12451943
申请日：2008-06-06
申请人： Theo Devos , Cathy Lofton-Day , Andrew Sledziewski , Fabian Model , Michael Krouse , Jesse Ho , Matthias Schuster , Juergen Distler , Reimo Tetzner
发明人： Theo Devos , Cathy Lofton-Day , Andrew Sledziewski , Fabian Model , Michael Krouse , Jesse Ho , Matthias Schuster , Juergen Distler , Reimo Tetzner
IPC分类号： C40B30/00 , G01N33/53 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6813 , C12Q1/6806 , C12Q1/6827 , C12Q1/6844 , C12Q2523/125
摘要： Aspects of the invention relate to composition and methods for the providing of DNA for methylation analysis that is in particular suitable to be applied in reference laboratories. Further 5 aspects of the invention relate to composition and methods for the highly specific and sensitive methylation analysis of the Septin 9 gene also in particular suitable to be applied in reference laboratories.
摘要翻译：本发明的方面涉及提供用于甲基化分析的DNA的组合物和方法，其特别适用于参考实验室。本发明的另外5个方面涉及用于Septin 9基因的高度特异性和敏感的甲基化分析的组合物和方法，特别适用于参考实验室。

9. 发明申请

US20100184027A1 METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS 审中-公开
标题翻译：用于细胞增殖性疾病分析的方法和核酸
公开(公告)号：US20100184027A1
公开(公告)日：2010-07-22
申请号：US12373709
申请日：2007-07-13
申请人： Catherine Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
发明人： Catherine Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/04
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/154
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测或检测和区分增殖性疾病之间或之中的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

10. 发明申请

US20100143902A1 METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS 审中-公开
标题翻译：用于细胞增殖性疾病分析的方法和核酸
公开(公告)号：US20100143902A1
公开(公告)日：2010-06-10
申请号：US12374654
申请日：2007-07-23
申请人： Catherine E Lofton-Day , Andrew Z. Sledziewski , Fabian Model , Susan Cottrell , Juergen Distler , Reimo Tetzner , Dimo Dietrich
发明人： Catherine E Lofton-Day , Andrew Z. Sledziewski , Fabian Model , Susan Cottrell , Juergen Distler , Reimo Tetzner , Dimo Dietrich
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6886 , C12Q2600/154
摘要： The invention provides methods, nucleic acids and kits for detecting colorectal cell proliferative disorders based on underexpression or methylation of a least one gene selected from RASSF2, TFAP2E, SND1, PCDHGC3, EDNRB, STOM, GLI3, RXFP3, LimK1, GPR73L1, PCDH1O, DOCKIO and MRPS21, and optionally Septin-9. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测基于选自RASSF2，TFAP2E，SND1，PCDHGC3，EDNRB，STOM，GLI3，RXFP3，LimK1，GPR73L1，PCDH1O，DOCKIO中的至少一种基因的低表达或甲基化的结肠直肠细胞增殖性疾病的方法，核酸和试剂盒和MRPS21，以及任选的Septin-9。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测，从而能够改善患者的诊断和治疗。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式