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1. 发明授权

US4902622A Novel .alpha.-1,6-glucosidase and process for producing the same 失效
标题翻译：新型α-1,6-葡糖苷酶及其制备方法
公开(公告)号：US4902622A
公开(公告)日：1990-02-20
申请号：US100730
申请日：1987-09-25
申请人： Kuniharu Nakai , Masayoshi Shiozaki , Hiroji Tsuji , Shigeharu Mori , Susumu Hirose , Nobumasa Yokoi , Takaichi Ohya
发明人： Kuniharu Nakai , Masayoshi Shiozaki , Hiroji Tsuji , Shigeharu Mori , Susumu Hirose , Nobumasa Yokoi , Takaichi Ohya
IPC分类号： C12N9/44
CPC分类号： C12R1/07 , C12N9/2451 , Y10S435/832
摘要： A novel .alpha.-1,6-glucosidase having the following properties:(1) Action: The enzyme acts on an .alpha.-1,6-glucoside bond to produce linear amylose;(2) Substrate specificity: The enzyme has the following relative activity to polysaccharides:______________________________________ Polysaccharide Relative activity (%) ______________________________________ Pullulan 100 Maize soluble amylopectin 13.5 Soluble starch 6.7 Oyster glycogen 1.7 Hare glycogen 2.6 Maize amylopectin 3.8 Potato amylopectin 4.0 ______________________________________ (3) Km value and Vmax to pullulan: the enzyme has Km value of 0.14 mg/ml and Vmax of 70.0 .mu.mol/min/mg protein;(4) Optimum pH: 5.0 to 5.5;(5) pH stability: The enzyme is stable in the pH range of 4.5 to 6.5 for 30 minutes at 40.degree. C. and in the pH range of 5.0 to 6.0 for 30 minutes at 50.degree. C.;(6) Optimum temperature: Approximately 55.degree. C.;(7) Thermal stability: Treatment at pH 4.5 for 30 minutes at different temperatures indicates that the enzyme retained 80% of the initial activity at 40.degree. C. and 10% thereof at 60.degree. C.;(8) Influence of inhibitor: The enzyme is inhibited by at least 70% by p-chloro-mercuribenzoic acid or sodium dodecylsulfate, but is not inhibited by o-phenanthroline or potassium ferricyanide;(9) Influence of metal salts: The enzyme is negligibly affected by Ni.sup.2+, Ba.sup.2+,Zn.sup.2+, Cd.sup.2+ or Mn.sup.2+ ; is inhibited by Fe.sup.3+ ; and loses activity in the presence of Hg.sup.2+ or Ag.sup.2+ ; and(10) Molecular Weight: About 95,500 (measured by SDS electrophoresis).
摘要翻译：具有以下性质的新型α-1,6-葡糖苷酶：（1）作用：酶作用于α-1,6葡萄糖苷键以产生线性直链淀粉; （2）底物特异性：该酶对多糖具有以下相对活性： - 多糖相对活性（％） - 支链淀粉100-可溶性支链淀粉13.5-可溶淀粉6.7-牡蛎糖原1.7-高糖糖原2.6-支链淀粉3.8-支链淀粉 4.0 - （3）Km值和Vmax至支链淀粉：酶的Km值为0.14mg / ml，Vmax为70.0μmol/ min / mg蛋白质; （4）最适pH：5.0〜5.5; （5）pH稳定性：该酶在40℃，pH5.0〜6.0的pH范围为4.5〜6.5，在50℃，pH为5.0〜6.0，稳定30分钟。（6）最佳温度：约55℃。（7）热稳定性：在不同温度下在pH 4.5下处理30分钟表明酶在40℃下保留80％的初始活性，在60℃下保留10％的初始活性。（8）抑制剂的影响：该酶被对氯 - 汞二苯甲酸或十二烷基硫酸钠抑制至少70％，但不被邻菲咯啉或铁氰化钾抑制; （9）金属盐的影响：酶被Ni2 +，Ba2 +，Zn2 +，Cd2 +或Mn2 +忽略不计; 被Fe3 +抑制; 并在Hg2 +或Ag2 +存在下失去活性; 和（10）分子量：约95,500（通过SDS电泳测量）。

2. 发明授权

US3983002A Process for preparation of cellulase 失效
标题翻译：纤维素酶的制备方法
公开(公告)号：US3983002A
公开(公告)日：1976-09-28
申请号：US522262
申请日：1974-11-08
申请人： Takaichi Ohya , Nobumasa Yokoi , Tamio Mase
发明人： Takaichi Ohya , Nobumasa Yokoi , Tamio Mase
IPC分类号： A23F5/16 , C12N9/42 , C12D13/10 , C07G7/02
CPC分类号： C12N9/2437 , A23F5/163 , C12N9/2477 , Y10S435/822
摘要： Aeromonas sp. No. 212 is cultured to allow it to produce a novel cellulase 212 and/or novel hemicellulase in a culture medium, and the so produced cellulase 212 and/or hemicellulase 333 is separated from the culture medium.The so separated novel cellulase 212 and/or novel hemicellulase 333 is used as a cellulosic material-decomposing agent in purifying tanks, plants for treatment of industrial waste waters, excrement treatment plants, drainage disposal treatment plants and the like.
摘要翻译：气单胞菌属培养编号212以使其在培养基中产生新的纤维素酶212和/或新型半纤维素酶，并且将如此制备的纤维素酶212和/或半纤维素酶333与培养基分离。

3. 发明授权

US5707833A Process of making cyclodextrin glucanotransferase with brevibacterium 失效
标题翻译：用短杆菌制备环糊精葡聚糖转移酶的方法
公开(公告)号：US5707833A
公开(公告)日：1998-01-13
申请号：US471235
申请日：1995-06-06
申请人： Shigeharu Mori , Tamio Mase , Takaichi Ohya
发明人： Shigeharu Mori , Tamio Mase , Takaichi Ohya
IPC分类号： C12N9/10 , C12P19/18 , C12P21/04
CPC分类号： C12N9/1074 , C12P19/18 , Y10S435/84
摘要： This invention relates to a .gamma.-cyclodextrin glucanotransferase having novel properties, to a process for the production of the .gamma.-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the .gamma.-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of .gamma.-cyclodextrin as a main product, and to a method for increasing .gamma.-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which .gamma.-cyclodextrin and .beta.-cyclodextrin are formed from starch by the action of .gamma.-cyclodextrin glucanotransferase, thereby effecting repressed formation of .beta.-cyclodextrin and enhanced formation of .gamma.-cyclodextrin. Development of the process of this invention has rendered possible low cost production of .gamma.-cyclodextrin and therefore expands practical use of .gamma.-cyclodextrin to the field of food and the like.
摘要翻译：本发明涉及一种具有新特性的γ-环糊精葡聚糖转移酶，涉及γ-环糊精葡聚糖转移酶的制备方法，该方法包括培养能产生环糊精葡聚糖转移酶的短杆菌属属的菌株，从而使菌株产生γ- 环糊精葡聚糖转移酶，然后将酶收集到生产环糊精的方法中，该方法包括使环糊精葡聚糖转移酶与溶解在溶液中的底物反应，从而形成γ-环糊精作为主要产物，并且一种增加γ-环糊精产率而不伴随产生总环糊精的增加的方法，该方法包括向γ-淀粉酶通过γ-环糊精葡聚糖转移酶的作用从淀粉形成γ-环糊精和β-环糊精的反应溶液中加入乙醇，形成β-环糊精并增强γ-环糊精的形成。本发明方法的开发使得γ-环糊精的低成本生产成为可能，从而将γ-环糊精的实际应用扩展到食品领域等。

4. 发明授权

US5124262A Mannose isomerase and process for mannose production using it 失效
标题翻译：甘露糖异构酶和使用它的甘露糖生产方法
公开(公告)号：US5124262A
公开(公告)日：1992-06-23
申请号：US661139
申请日：1991-02-27
申请人： Yoshiyuki Takasaki , Takaichi Ohya
发明人： Yoshiyuki Takasaki , Takaichi Ohya
IPC分类号： C12N9/90
CPC分类号： C12R1/38 , C12N9/90
摘要： A mannose isomerase having excellent properties for industrial use, such as high thermal stability and resistance to high substrate concentrations, can be produced by culturing a strain of Pseudomonas (sp. AM-9582), and extracting it from the cells of AM-9582. Mannose can be effectively produced from fructose of high concentrations using the enzyme.
摘要翻译：可以通过培养假单胞菌（SP.AM-9582）菌株并从AM-9582的细胞中提取具有优异工业性质的甘露糖异构酶，例如高热稳定性和高底物浓度。使用酶可以从高浓度的果糖有效地制备甘露糖。

5. 发明授权

US5352602A Isoamylase and process for producing the same 失效
标题翻译：异淀粉酶及其制备方法
公开(公告)号：US5352602A
公开(公告)日：1994-10-04
申请号：US24072
申请日：1993-03-01
申请人： Yuzo Yamada , Toshihiro Sato , Takaichi Ohya
发明人： Yuzo Yamada , Toshihiro Sato , Takaichi Ohya
IPC分类号： C12N9/44 , C12R1/64 , C12P19/16
CPC分类号： C12N9/246 , C12Y302/01068 , Y10S435/91
摘要： An isoamylase, useful in the field of starch saccharification, having a molecular weight of about 105,000, an isoelectric point of 6.4, an optimum pH of 3.0 to 5.0, an optimum temperature of about 50.degree. C. and exhibiting temperature stability at 45.degree. C..times.10 minutes and pH stability at pH 3.5 to 6.0, and a process for producing the same comprising cultivating an isoamylase-producing strain belonging to the genus Xanthomonas and recovering the enzyme produced.
摘要翻译：可用于淀粉糖化领域的异淀粉酶，其分子量约为105,000，等电点为6.4，最适pH为3.0至5.0，最适温度为约50℃，温度稳定在45℃ pH为3.5〜6.0的pH稳定性及其制造方法，包括培养属于黄单胞菌属的异淀粉酶生产菌株，回收生成的酶。

6. 发明授权

US5240717A Process for mannose and mannose isomerase production using mannose isomerase-producing pseudomonas 失效
标题翻译：使用产甘露糖异构酶的假单胞菌生产甘露糖和甘露糖异构酶的方法
公开(公告)号：US5240717A
公开(公告)日：1993-08-31
申请号：US851665
申请日：1992-03-16
申请人： Yoshiyuki Takasaki , Takaichi Ohya
发明人： Yoshiyuki Takasaki , Takaichi Ohya
IPC分类号： C12N9/90
CPC分类号： C12R1/38 , C12N9/90 , Y10S435/874
摘要： A mannose isomerase having excellent properties for industrial use, such as high thermal stability and resistance to high substrate concentrations, can be produced by culturing a strain of Pseudomonas (sp. AM-9582), and extracting it from the cells of AM-9582. Mannose can be effectively produced from fructose of high concentrations using the enzyme.
摘要翻译：可以通过培养假单胞菌（SP.AM-9582）菌株并从AM-9582的细胞中提取具有优异工业性质的甘露糖异构酶，例如高热稳定性和高底物浓度。使用酶可以从高浓度的果糖有效地制备甘露糖。

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