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1. 发明申请

WO2005052135A1 NOVEL RUMEN BACTERIA VARIANTS AND PROCESS FOR PREPARING SUCCINIC ACID EMPLOYING THE SAME 审中-公开
标题翻译：新型葡萄酒细菌变异体及其制备使用同系酸的方法
公开(公告)号：WO2005052135A1
公开(公告)日：2005-06-09
申请号：PCT/KR2004/001210
申请日：2004-05-20
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Sang Jun
发明人： LEE, Sang Yup , LEE, Sang Jun
IPC分类号： C12N1/21
CPC分类号： C12P7/46 , C12N1/20 , C12R1/01
摘要： The present invention relates to novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene ( ldhA ) and a pyruvate formate-lyase gene ( pf l) (which are involved in the production of lactic acid, formic acid and acetic acid) from rumen bacteria; a novel bacterial mutant ( Mannheimia sp. LPK7) having disruptions of a lactate dehydrogenase gene ( ldhA ), a pyruvate formate-lyase gene ( plf ), a phosphotransacetylase gene ( pta ), and a acetate kinase gene ( ackA ); a novel bacterial mutant ( Mannheimia sp. LPK4) having disruptions of a lactate dehydrogenase gene ( ldhA ), a pyruvate formate-lyase gene ( pfl ) and a phosphoenolpyruvate carboxylase gene ( ppc ) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, which is characterized by the culture of the above mutants in anaerobic conditions. The inventive bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the inventive bacterial mutants are useful as strains for the industrial production of succinic acid.
摘要翻译：本发明涉及由瘤胃中的乳酸脱氢酶基因（ldhA）和丙酮酸甲酸裂解酶基因（pfl）（其涉及乳酸，甲酸和乙酸的生产）的破坏而产生的新型瘤胃细菌突变体菌; 具有乳酸脱氢酶基因（ldhA），丙酮酸甲酸裂合酶基因（plf），磷酸转乙酰酶基因（pta）和乙酸激酶基因（ackA）的破坏的新型细菌突变体（曼氏血清杆菌LPK7）; 具有破坏乳酸脱氢酶基因（ldhA），丙酮酸甲酸裂合酶基因（pfl）和磷酸烯醇丙酮酸羧化酶基因（ppc）的新型细菌突变体（曼氏血吸虫属LPK4），其涉及将CO 2固定在生产代谢途径中琥珀酸; 以及生产琥珀酸的方法，其特征在于在厌氧条件下培养上述突变体。与生产各种有机酸的现有野生型菌株相比，本发明的细菌突变体具有以高浓度生产琥珀酸的同时产生很少或不含有机酸的性质。因此，本发明的细菌突变体可用作工业生产琥珀酸的菌株。

2. 发明申请

WO2006107127A1 METHOD FOR IMPROVING A STRAIN BASED ON IN-SILICO ANALYSIS 审中-公开
标题翻译：用于改进基于硅分析的应变的方法
公开(公告)号：WO2006107127A1
公开(公告)日：2006-10-12
申请号：PCT/KR2005/001501
申请日：2005-05-23
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , KIM, Tae Yong , LEE, Dong Yup , LEE, Sang Jun
发明人： LEE, Sang Yup , KIM, Tae Yong , LEE, Dong Yup , LEE, Sang Jun
IPC分类号： C12N1/00
CPC分类号： C12P7/46 , C12N9/1205 , C12N15/1089
摘要： The present invention is related to a method for improving a strain on the basis of in silico analysis, in which it compares the genomic information of a target strain for producing a useful substance to the genomic information of a strain overproducing the useful substance so as to primarily screen genes unnecessary for the overproduction of the useful substance, and then to secondarily screen genes to be deleted through performing simulation with metabolic flux analysis. According to the present invention, an improved strain can be effectively constructed by the metabolic and genetic engineering approach comprising comparatively analyzing the genomic information of a target strain for producing a useful substance and the genomic information of a strain producing a large amount of the useful substance to screen candidate genes and performing in silico simulation on the screened candidate genes to select a combination of genes to be deleted, which shows an improvement in the production of the useful substance. Accordingly, the time, effort and cost required for an actual wet test can be significantly reduced.
摘要翻译：本发明涉及一种基于在计算机分析中改进菌株的方法，其中将用于产生有用物质的靶菌株的基因组信息与过量产生有用物质的菌株的基因组信息进行比较，以便主要是用于过量生产有用物质的筛选基因，然后通过用代谢通量分析进行模拟来次要筛选待缺失的基因。根据本发明，可以通过代谢和基因工程方法有效地构建改进的菌株，其包括比较分析用于产生有用物质的靶菌株的基因组信息和产生大量有用物质的菌株的基因组信息筛选候选基因并在筛选的候选基因的计算机模拟中进行选择要缺失的基因的组合，其显示有用物质的生产的改善。因此，可以显着降低实际湿测试所需的时间，精力和成本。

3. 发明公开

EP1692271A1 NOVEL RUMEN BACTERIA VARIANTS AND PROCESS FOR PREPARING SUCCINIC ACID EMPLOYING THE SAME 有权转让
标题翻译： NEW几乎阿根细菌变异体和工艺琥珀酸及其制备使用
公开(公告)号：EP1692271A1
公开(公告)日：2006-08-23
申请号：EP04734158.1
申请日：2004-05-20
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , LEE, Sang Jun
IPC分类号： C12N1/21
CPC分类号： C12P7/46 , C12N1/20 , C12R1/01
摘要： The present invention relates to novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene (ldhA) and a pyruvate formate-lyase gene (pfl) (which are involved in the production of lactic acid, formic acid and acetic acid) from rumen bacteria; a novel bacterial mutant (Mannheimia sp. LPK7) having disruptions of a lactate dehydrogenase gene (ldhA), a pyruvate formate-lyase gene (plf), a phosphotransacetylase gene (pta), and a acetate kinase gene (ackA); a novel bacterial mutant (Mannheimia sp. LPK4) having disruptions of a lactate dehydrogenase gene (ldhA), a pyruvate formate-lyase gene (pfl) and a phosphoenolpyruvate carboxylase gene (ppc) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, which is characterized by the culture of the above mutants in anaerobic conditions. The inventive bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the inventive bacterial mutants are useful as strains for the industrial production of succinic acid.

4. 发明授权

EP1692271B1 NOVEL RUMEN BACTERIA VARIANTS AND PROCESS FOR PREPARING SUCCINIC ACID EMPLOYING THE SAME 有权转让
标题翻译： NEW几乎阿根细菌变异体和工艺琥珀酸及其制备使用
公开(公告)号：EP1692271B1
公开(公告)日：2009-08-12
申请号：EP04734158.1
申请日：2004-05-20
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , LEE, Sang Jun
IPC分类号： C12N1/21 , C12P7/46 , C12N15/63 , C12N15/74 , C12R1/00
CPC分类号： C12P7/46 , C12N1/20 , C12R1/01

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