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1. 发明申请

US20080299636A1 SYSTEM AND METHOD FOR INACTIVATING MICROORGANISMS WITH A FEMTOSECOND LASER 审中-公开
标题翻译：用FEMTOSECOND激光灭活微生物的系统和方法
公开(公告)号：US20080299636A1
公开(公告)日：2008-12-04
申请号：US12131710
申请日：2008-06-02
申请人： Kong-Thon Tsen , Shaw-Wei D. Tsen , Juliann G. Kiang
发明人： Kong-Thon Tsen , Shaw-Wei D. Tsen , Juliann G. Kiang
IPC分类号： C12N13/00 , H01S3/10
CPC分类号： C12N13/00 , C12M35/02 , C12N1/36
摘要： The invention relates to a method of inactivating or diminishing the activity of microorganisms and an apparatus implementing that method. Microorganism activity is diminished through exciting the vibrational state of a microorganism with pulses of radiation femtoseconds in width. The wavelength of the pulses is in a range of the electromagnetic spectrum to which water is substantially transparent such as visible light. The pulses cause the microorganisms to vibrate such that their activities become diminished. A laser produces the pulses. A harmonic generator then acts upon the pulses to produce a scattering effect that is used to irradiate the microorganisms. One such laser is a titanium sapphire laser. One example of a harmonic generator is a nonlinear crystal such as a BBO crystal. The apparatus may also have a focusing lens such as a microscope objective that focuses the beam on a microorganism. The invention is effective for the inactivation of viruses or bacteria. The present invention may be utilized in order for extracting nucleic acid from microorganisms. The invention may be used in the manufacturing of vaccines. The invention enables the selective inactivation of target viruses and bacteria without causing cytotoxicity in mammalian cells.
摘要翻译：本发明涉及灭活或减少微生物活性的方法以及实施该方法的装置。通过激发具有宽度的辐射飞秒脉冲的微生物的振动状态，微生物活性减弱。脉冲的波长在水基本上透明的电磁光谱的范围内，例如可见光。脉冲导致微生物振动，使得它们的活动减弱。激光产生脉冲。然后，谐波发生器作用于脉冲以产生用于照射微生物的散射效应。一种这样的激光器是钛蓝宝石激光器。谐波发生器的一个例子是非线性晶体，例如BBO晶体。该装置还可以具有聚焦透镜，例如将光束聚焦在微生物上的显微镜物镜。本发明对于灭活病毒或细菌是有效的。为了从微生物中提取核酸，可以使用本发明。本发明可用于制造疫苗。本发明使得靶细胞和细菌的选择性失活而不会在哺乳动物细胞中引起细胞毒性。

2. 发明申请

US20100136646A1 Selective Inactivation Of Microorganisms With A Femtosecond Laser 审中-公开
标题翻译：微生物与飞秒激光的选择性失活
公开(公告)号：US20100136646A1
公开(公告)日：2010-06-03
申请号：US12646961
申请日：2009-12-23
申请人： Kong-Thon Tsen , Shaw-Wei D. Tsen , Juliann G. Kiang
发明人： Kong-Thon Tsen , Shaw-Wei D. Tsen , Juliann G. Kiang
IPC分类号： C12N13/00
CPC分类号： C12N7/00 , C12N13/00 , C12N2795/00061
摘要： A method is provided for selectively inactivating microorganisms with femtosecond pulsed lasers. Under proper laser conditions, irradiation of the femtosecond pulsed laser causes inactivation of pathogenic microorganisms, for example, viruses, bacteria and protozoa, without causing cytotoxicity in mammalian cells. Pathogenic microorganism activity is diminished through an impulsive stimulated Raman scattering process, that is, through the excitation of the low-energy vibrational state on the outer structure of a microorganism with femtosecond pulsed lasers. The wavelength of the laser pulses is in a range of the electromagnetic spectrum, for example, visible and near-infrared where water is substantially transparent. The method is utilized for cleansing blood components, disinfecting drinking water, treating viral and bacterial diseases, extracting nucleic acid from microorganisms, and for manufacturing vaccines.
摘要翻译：提供了用飞秒脉冲激光选择性灭活微生物的方法。在适当的激光条件下，飞秒脉冲激光的照射导致致病微生物，例如病毒，细菌和原生动物的灭活，而不会在哺乳动物细胞中引起细胞毒性。通过脉冲激发的拉曼散射过程，即通过用飞秒脉冲激光激发微生物的外部结构上的低能量振动状态，致病微生物活性降低。激光脉冲的波长在电磁光谱的范围内，例如可见光和近红外光，其中水基本上是透明的。该方法用于清洗血液成分，消毒饮用水，治疗病毒和细菌疾病，从微生物中提取核酸和制造疫苗。

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