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1. 发明授权

US5994629A Positive selection 失效
标题翻译：积极选择
公开(公告)号：US5994629A
公开(公告)日：1999-11-30
申请号：US527474
申请日：1995-09-13
申请人： Kirsten Bojsen , Iain Donaldson , Anna Haldrup , Morten Joersboe , Jette D. Kreiberg , John Nielsen , Finn T. Okkels , Steen G. Petersen , Robert J. Whenham
发明人： Kirsten Bojsen , Iain Donaldson , Anna Haldrup , Morten Joersboe , Jette D. Kreiberg , John Nielsen , Finn T. Okkels , Steen G. Petersen , Robert J. Whenham
IPC分类号： A01H5/12 , C07H15/203 , C07H15/26 , C07H17/02 , C07H19/16 , C07H19/20 , C12N5/00 , C12N9/24 , C12N9/90 , C12N9/92 , C12N15/65 , C12N15/82 , C12N15/00
CPC分类号： C07H15/203 , A01H5/12 , C07H15/26 , C07H17/02 , C07H19/16 , C07H19/20 , C12N15/65 , C12N15/8209 , C12N15/8245 , C12N5/0025 , C12N9/2402 , C12N9/90 , C12N9/92 , C12Y302/01031
摘要： A method of selecting genetically transformed cells from a population of cells comprising introducing a desired nucleotide sequence and a co-introduced nucleotide sequence into the genome of a cell whereby the desired nucleotide sequence or the co-introduced nucletoide sequence induces a positive effect by giving the transformed cells a competitive advantage when the population of cells are supplied with an inactive compound thereby allowing the transformed cells to be identified and selected from the non-transformed cells by means defined as positive selection; as well as cells transformed according to the method and plants derived therefrom. The invention further relates to novel glucuronide compounds including cytokinin glucuronide compounds for use in the method.
摘要翻译：从细胞群中选择遗传转化的细胞的方法，包括将期望的核苷酸序列和共同引入的核苷酸序列引入细胞的基因组，由此所需的核苷酸序列或共同引入的核苷序列通过给予当细胞群体被供应无活性化合物从而允许通过定义为阳性选择的方式从非转化细胞中鉴定和选择转化的细胞时，转化细胞具有竞争优势; 以及根据方法转化的细胞和从其衍生的植物。本发明还涉及包含用于该方法的细胞分裂素葡糖苷酸化合物的新型葡糖苷酸化合物。

2. 发明授权

US5767378A Mannose or xylose based positive selection 失效
标题翻译：甘露糖或木糖为阳性选择
公开(公告)号：US5767378A
公开(公告)日：1998-06-16
申请号：US505302
申请日：1995-10-03
申请人： Kirsten Bojsen , Iain Donaldson , Anna Haldrup , Morten Joersboe , Jette Dina Kreiberg , John Nielsen , Finn Thyge Okkels , Steen Guldager Petersen
发明人： Kirsten Bojsen , Iain Donaldson , Anna Haldrup , Morten Joersboe , Jette Dina Kreiberg , John Nielsen , Finn Thyge Okkels , Steen Guldager Petersen
IPC分类号： A01H5/00 , C07H15/203 , C07H15/26 , C07H17/02 , C07H19/16 , C07H19/20 , C12N5/00 , C12N5/10 , C12N9/24 , C12N9/90 , C12N9/92 , C12N15/09 , C12N15/29 , C12N15/65 , C12N15/82 , C12Q1/02 , C12N15/31
CPC分类号： C07H15/203 , C07H15/26 , C07H17/02 , C07H19/16 , C07H19/20 , C12N15/65 , C12N15/8209 , C12N15/8245 , C12N5/0025 , C12N9/90 , C12N9/92 , C12Y503/01008
摘要： The present invention provides a method for identifying or selecting from a population of eukaryotic cells cultivated on or in a medium containing at least one compound, cells which have a metabolic advantage as a result of having being transformed, wherein: i) the cells are transformed with a nucleotide sequence or a co-introduced nucleotide sequence one of which comprises a region which: (a) encodes a protein which is involved in the metabolism of the compound, and/or (b) regulates the activity of the protein; and ii) the compound is mannose or xylose or a derivative or a precursor of these, or a substrate of the protein, or is capable of being metabolized by the transformed cells into such a substrate, with the proviso that the compound is not mannose when the protein is mannose 6 phosphate isomerase.The invention also includes a method according to the preceding paragraph wherein the compounds are not so limited with the proviso that an agent which reduces the toxicity to the cells of the compound is added to the medium. It is preferred that where a toxicity-reducing agent has been added to the culture medium, the compound is mannose and the nucleotide or co-introduced nucleotide sequence encodes mannose-6-phosphate isomerase.
摘要翻译： PCT No.PCT / EP94 / 00575 Sec。 371 1995年10月3日第 102（e）日期1995年10月3日PCT 1994年2月28日PCT PCT。公开号WO94 / 20627 1994年9月15日本发明提供了鉴定或选择在含有至少一种化合物的培养基上或培养基中培养的真核细胞群体的方法，所述培养基具有作为转化结果具有代谢优势的细胞，其中：i ），用核苷酸序列或共同引入的核苷酸序列转化细胞，其中之一包括以下区域：（a）编码参与化合物代谢的蛋白质，和/或（b）调节活性蛋白质; 和ii）化合物是甘露糖或木糖，或其衍生物或前体，或蛋白质的底物，或能够被转化的细胞代谢成这样的底物，条件是该化合物不是甘露糖，当蛋白质是甘露糖6磷酸异构酶。本发明还包括根据前述段落的方法，其中化合物不限于此，条件是向培养基中加入降低化合物细胞毒性的试剂。优选的是，当向培养基中加入毒性降低剂时，化合物是甘露糖，核苷酸或共同引入的核苷酸序列编码甘露糖-6-磷酸异构酶。

3. 发明授权

US06632643B2 Use of &agr;-1,4-glucan lyase for preparation of 1,5-D-hydrofructose 失效
标题翻译：使用α-1,4-葡聚糖裂解酶制备1,5-脱氢果糖
公开(公告)号：US06632643B2
公开(公告)日：2003-10-14
申请号：US09280197
申请日：1999-03-29
申请人： Shukun Yu , Kirsten Bojsen , Karsten Kragh , Maja Bojko , John Nielsen , Jan Marcussen , Tove Christensen
发明人： Shukun Yu , Kirsten Bojsen , Karsten Kragh , Maja Bojko , John Nielsen , Jan Marcussen , Tove Christensen
IPC分类号： C12P902
CPC分类号： C12P19/02 , C12N9/00 , C12N9/88
摘要： A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an &agr;-1,4-glucan with an &agr;-1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the &agr;-1,4-links, the &agr;-1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.
摘要翻译：描述了制备糖1,5-脱水果糖的方法。该方法包括用α-1,4-葡聚糖裂解酶处理α-1,4-葡聚糖，其中酶以基本上纯的形式使用。在优选的实施方案中，如果葡聚糖含有除了α-1,4-链节以外的链接，则将α-1,4-葡聚糖裂合酶与可以破坏其它链接的合适的试剂结合使用。 / PTEXT>

4. 发明授权

US6013504A .alpha.-1,4-glucan lyase from a fungus infected algae, its purification, gene cloning and expression in microorganisms 失效
标题翻译：来自真菌感染藻类的α-1,4-葡聚糖裂解酶，其纯化，基因克隆和在微生物中的表达
公开(公告)号：US6013504A
公开(公告)日：2000-01-11
申请号：US633768
申请日：1996-07-02
申请人： Shukun Yu , Kirsten Bojsen , Karsten Kragh , Maja Bojko , John Nielsen , Jan Marcussen
发明人： Shukun Yu , Kirsten Bojsen , Karsten Kragh , Maja Bojko , John Nielsen , Jan Marcussen
IPC分类号： C12N15/09 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/88 , C12N15/60 , C12P19/02 , C12R1/19 , C12R1/685 , C12R1/84 , C12R1/89 , C12R1/90
CPC分类号： C12P19/02 , C12N9/00 , C12N9/88
摘要： A method of preparing .alpha.-1,4-glucan lyase enzymes is described. The method comprises isolating the enzymes from a fungally infected algae. The amino acid sequences of the enzymes have been determined. The nucleic acid sequences coding for the enzymes have also been determined.
摘要翻译： PCT No.PCT / EP94 / 03399 Sec。 371日期：1996年7月2日 102（e）日期1996年7月2日PCT 1994年10月15日PCT PCT。公开号WO95 / 10618 日期1996年4月20日描述了制备α-1,4-葡聚糖裂解酶的方法。该方法包括从真菌感染的藻类中分离酶。已经确定了酶的氨基酸序列。也已经确定了编码酶的核酸序列。

5. 发明授权

US08509790B2 Multi-speed mesh networks 有权
标题翻译：多速网状网络
公开(公告)号：US08509790B2
公开(公告)日：2013-08-13
申请号：US11425917
申请日：2006-06-22
申请人： Tommas Jess Christensen , Jacob Midtgaard , Didier Clement , John Nielsen , Morten Vested Olesen , Olfert Paul Paulsen , Rasmus Glarborg Jensen
发明人： Tommas Jess Christensen , Jacob Midtgaard , Didier Clement , John Nielsen , Morten Vested Olesen , Olfert Paul Paulsen , Rasmus Glarborg Jensen
IPC分类号： H04W72/00
CPC分类号： H04L29/06027 , H04L1/189 , H04L63/0853 , H04L65/607 , H04L2001/0093 , H04W28/22 , H04W92/02
摘要： In embodiments of the present invention improved capabilities are described for a communications facility that is adapted to communicate with at least one other mesh network node at a communication speed selected from a plurality of communication speeds.
摘要翻译：在本发明的实施例中，针对适于以从多个通信速度选择的通信速度与至少一个其他网状网络节点进行通信的通信设施来描述改进的能力。

6. 发明授权

US08340588B2 Handheld synthetic antenna array 失效
标题翻译：手持式合成天线阵列
公开(公告)号：US08340588B2
公开(公告)日：2012-12-25
申请号：US12585605
申请日：2009-09-18
申请人： John Nielsen , Gerard Lachapelle , Ali Broumandan
发明人： John Nielsen , Gerard Lachapelle , Ali Broumandan
IPC分类号： H04B17/00
CPC分类号： H04B7/0891 , H04B7/028 , H04B7/086
摘要： A system for estimating parameters of an incoming signal is provided. At least one antenna is coupled to at least one suitable receiver. The antenna(s) are spatially translated in an arbitrary trajectory. As the antenna(s) is being spatially translated, a data processing means samples the incoming signal at set intervals based on a clock signal provided by a system clock. By sampling the incoming signal at different times at different spatial locations on the arbitrary trajectory, the system acts as a synthetic antenna array. The different samplings of the incoming signal at different times and positions provide signal diversity gain as well as different readings which can be used to estimate and/or calculate various parameters of the incoming signal. The different samplings can be used to detect the incoming signal, estimate its angle of arrival, estimate its time of arrival, as well as other parameters.
摘要翻译：提供了一种用于估计输入信号的参数的系统。至少一个天线耦合到至少一个合适的接收机。天线在任意轨迹上空间转换。当天线被空间转换时，数据处理装置基于由系统时钟提供的时钟信号以设定的时间间隔对输入信号进行采样。通过在任意轨迹上的不同空间位置的不同时间采样输入信号，该系统充当合成天线阵列。在不同时间和位置处的输入信号的不同采样提供信号分集增益以及可用于估计和/或计算输入信号的各种参数的不同读数。不同的采样可用于检测输入信号，估计其到达角度，估计其到达时间以及其他参数。

7. 发明申请

US20110070857A1 SIGNAL DETECTION IN FADING ENVIRONMENTS 失效
标题翻译：衰落环境中的信号检测
公开(公告)号：US20110070857A1
公开(公告)日：2011-03-24
申请号：US12884360
申请日：2010-09-17
申请人： John Nielsen , Gerard Lachapelle , Ali Broumandan
发明人： John Nielsen , Gerard Lachapelle , Ali Broumandan
IPC分类号： H04B1/10
CPC分类号： H04L25/0204 , H04B7/084 , H04L25/03012
摘要： Systems and methods related to the detection of incoming wireless signals. An antenna array is synthesized by having a single antenna, coupled to a receiver, spatially translated along an arbitrary trajectory. As the antenna is being spatially translated, a data processing means samples the incoming signal based on a clock signal provided by a system clock. By sampling the incoming signal at different times at different spatial locations on the arbitrary trajectory, the system acts as a synthetic antenna array. The different samplings of the incoming signal at different times and positions provide signal diversity gain as well as different readings which can be used to detect an incoming signal. The invention is applicable to detecting a wireless communications signal or a GNSS signal under various conditions. The system may include at least one sensor which can provide data for use in calculating data related to the arbitrary trajectory.
摘要翻译：与传入无线信号的检测相关的系统和方法。通过具有耦合到接收器的单个天线，沿着任意轨迹在空间上转换来合成天线阵列。当天线被空间转换时，数据处理装置根据由系统时钟提供的时钟信号对输入信号进行采样。通过在任意轨迹上的不同空间位置的不同时间采样输入信号，该系统充当合成天线阵列。在不同时间和位置的输入信号的不同采样提供信号分集增益以及可用于检测输入信号的不同读数。本发明适用于在各种条件下检测无线通信信号或GNSS信号。该系统可以包括至少一个传感器，其可以提供用于计算与任意轨迹有关的数据的数据。

8. 外观设计

USD558466S1 Portable cot 有权
标题翻译：便携式婴儿床
公开(公告)号：USD558466S1
公开(公告)日：2008-01-01
申请号：US29255950
申请日：2006-03-14
申请人： Jon Seddon , John Nielsen
设计人： Jon Seddon , John Nielsen

9. 发明申请

US20050080372A1 Wound dressing 有权
标题翻译：伤口敷料
公开(公告)号：US20050080372A1
公开(公告)日：2005-04-14
申请号：US10495433
申请日：2002-11-18
申请人： John Nielsen , Mette Kolte , Borge Gundersen
发明人： John Nielsen , Mette Kolte , Borge Gundersen
IPC分类号： A61F13/00 , A61F13/02
CPC分类号： A61F13/0203
摘要： A wound dressing comprising a backing layer, an adhesive layer and an absorbent layer between the backing layer and the adhesive layer, wherein the adhesive layer secures the absorbent layer to the backing layer and that the adhesive layer is interrupted in at least one zone enabling a direct contact between the absorbent layer and the backing layer and exposing at least a part of the skin contacting surface of the absorbent layer, characterised in that at least the skin-facing surface of the adhesive layer is provided with a non-detachable, non-adhesive cover layer.
摘要翻译：一种创伤敷料，其包括背衬层，粘合剂层和在背衬层和粘合剂层之间的吸收层，其中粘合剂层将吸收层固定到背衬层，并且粘合剂层在至少一个区域中断，使得能够在吸收层和背衬层之间直接接触并暴露吸收层的皮肤接触表面的至少一部分，其特征在于至少粘合剂层的面向皮肤的表面设置有不可拆卸的，粘合剂覆盖层。

10. 发明申请

US20110070840A1 Handheld synthetic antenna array 失效
标题翻译：手持式合成天线阵列
公开(公告)号：US20110070840A1
公开(公告)日：2011-03-24
申请号：US12585605
申请日：2009-09-18
申请人： John Nielsen , Gerard Lachapelle , Ali Broumandan
发明人： John Nielsen , Gerard Lachapelle , Ali Broumandan
IPC分类号： H04B17/00
CPC分类号： H04B7/0891 , H04B7/028 , H04B7/086
摘要： A system for estimating parameters of an incoming signal is provided. At least one antenna is coupled to at least one suitable receiver. The antenna(s) are spatially translated in an arbitrary trajectory. As the antenna(s) is being spatially translated, a data processing means samples the incoming signal at set intervals based on a clock signal provided by a system clock. By sampling the incoming signal at different times at different spatial locations on the arbitrary trajectory, the system acts as a synthetic antenna array. The different samplings of the incoming signal at different times and positions provide signal diversity gain as well as different readings which can be used to estimate and/or calculate various parameters of the incoming signal. The different samplings can be used to detect the incoming signal, estimate its angle of arrival, estimate its time of arrival, as well as other parameters.
摘要翻译：提供了一种用于估计输入信号的参数的系统。至少一个天线耦合到至少一个合适的接收机。天线在任意轨迹上空间转换。当天线被空间转换时，数据处理装置基于由系统时钟提供的时钟信号以设定的时间间隔对输入信号进行采样。通过在任意轨迹上的不同空间位置的不同时间采样输入信号，该系统充当合成天线阵列。在不同时间和位置处的输入信号的不同采样提供信号分集增益以及可用于估计和/或计算输入信号的各种参数的不同读数。不同的采样可用于检测输入信号，估计其到达角度，估计其到达时间以及其他参数。

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