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1. 发明授权

US6096530A Pseudomonas cepacia strain isolated from termite intestines that degrades trichlorethylene and furan compounds 失效
标题翻译：从白蚁肠分离的洋葱假单胞菌菌株，其分解三氯乙烯和呋喃化合物
公开(公告)号：US6096530A
公开(公告)日：2000-08-01
申请号：US810366
申请日：1997-03-03
申请人： Kinya Kato , Shinya Kozaki , Takeshi Imamura , Masanori Sakuranaga
发明人： Kinya Kato , Shinya Kozaki , Takeshi Imamura , Masanori Sakuranaga
IPC分类号： A62D3/00 , A62D3/02 , B09C1/10 , C02F3/12 , C02F3/34 , C12P1/04 , C12P17/04 , C12N1/20
CPC分类号： C12R1/38 , A62D3/02 , B09C1/10 , C02F3/1231 , C02F3/34 , C12P1/04 , C12P17/04 , A62D2101/28 , Y02W10/15
摘要： A biologically pure culture of Pseudomonas cepacia strain KK01 (FERM BP-4235) is capable of degrading trichloroethylene. A method for obtaining microorganisms having a trichloroethylene degrading ability comprises the steps of culturing microorganisms separated from the bodies of termites in a culture medium. A method for remediating a soil contaminated with trichloroethylene comprises the steps of providing a soil contaminated with trichloroethylene and bringing microorganisms having a trichloroethylene degrading ability derived from intestine of termites into contact with the soil, and biodegrading trichloroethylene in the soil. A method for biodegrading trichloroethylene, comprises the steps of culturing Pseudomonas cepacia KK01 (FERM BP-4235) under existence of an inducer and inducing Pseudomonas cepacia KK01 (FERM BP-4235) to have ability for degrading trichloroethylene and bringing Pseudomonas cepacia KK01 having trichloroethylene degrading ability to contact with trichloroethylene and biodegrading trichloroethylene.
摘要翻译：洋葱假单胞菌菌株KK01（FERM BP-4235）的生物学纯培养物能够降解三氯乙烯。获得具有三氯乙烯降解能力的微生物的方法包括在培养基中培养从白蚁体分离的微生物的步骤。用于补救被三氯乙烯污染的土壤的方法包括以下步骤：提供被三氯乙烯污染的土壤，并使来自白蚁肠的三氯乙烯降解能力的微生物与土壤接触，并在土壤中生物降解三氯乙烯。一种生物降解三氯乙烯的方法，包括在诱导物存在下培养洋葱假单胞菌KK01（FERM BP-4235）并诱导洋葱假单胞菌KK01（FERM BP-4235）具有降解三氯乙烯的能力，并使洋葱假单胞菌KK01具有三氯乙烯降解的步骤与三氯乙烯和生物降解三氯乙烯接触的能力。

2. 发明授权

US5658795A Method for biodegradation of polluting substance 失效
标题翻译：污染物质生物降解方法
公开(公告)号：US5658795A
公开(公告)日：1997-08-19
申请号：US571739
申请日：1995-12-13
申请人： Kinya Kato , Kazumi Tanaka , Masanori Sakuranaga , Shinya Kozaki
发明人： Kinya Kato , Kazumi Tanaka , Masanori Sakuranaga , Shinya Kozaki
IPC分类号： A62D3/02 , A62D3/00 , B09B3/00 , B09C1/10 , C02F3/00 , C02F3/10 , C02F3/34 , C12N1/00 , C12N1/20 , C07C61/00
CPC分类号： C02F3/34 , B09C1/10 , C02F3/108 , Y02W10/15
摘要： A method of biodegradation of a polluting substance by a microorganism is disclosed, wherein the microorganism is an auxotrophic microorganism and the action of the auxotrophic microorganism is controlled by the amount of a required nutrient for the auxotrophic microorganism. A carrier for supporting an auxotrophic microorganism for use for biodegradation contains a required nutrient for the auxotrophic microorganism.
摘要翻译：公开了一种微生物对污染物质进行生物降解的方法，其中微生物是营养缺陷型微生物，营养缺陷型微生物的作用是由营养缺陷型微生物所需营养素的量控制的。用于支持用于生物降解的营养缺陷型微生物的载体包含营养缺陷型微生物所需的营养物质。

3. 发明授权

US5863789A Microorganism-holding carrier and method for remediation of soil employing the carrier 失效
标题翻译：微生物保持载体和使用载体的土壤修复方法
公开(公告)号：US5863789A
公开(公告)日：1999-01-26
申请号：US899707
申请日：1997-07-24
申请人： Toshiyuki Komatsu , Masanori Sakuranaga , Tsuyoshi Nomoto , Shinya Kozaki , Takeshi Imamura
发明人： Toshiyuki Komatsu , Masanori Sakuranaga , Tsuyoshi Nomoto , Shinya Kozaki , Takeshi Imamura
IPC分类号： B09C1/10 , C12N11/00 , C12N11/04 , C12S9/00 , C12S13/00
CPC分类号： B09C1/10 , C12N11/00 , C12N11/04
摘要： A carrier for holding a microorganism in soil releases, from a constituting material thereof, an inducer for production of an enzyme of the microorganism for soil remediation. The carrier for holding a microorganism may comprise a combination of a microorganism holding carrier composed of a hydrophilic polymer for holding the microorganism with an inducer-holder composed of another polymer for holding inducer manifesting a biological action to the microorganism adjacent to each other. A method for remediation of soil comprises application of the microorganism-holding carrier into the soil. A soil-remedying agent comprises the microorganism-holding carrier and a microorganism held thereon which exhibits enzyme activity for decomposition of a polluting substance in the soil under action an inducer released by the carrier.
摘要翻译：用于将微生物保持在土壤中的载体从其构成材料中释放用于产生用于土壤修复的微生物的酶的诱导剂。用于保持微生物的载体可以包含由亲水性聚合物构成的微生物保持载体与微生物的组合，所述微生物保持载体由另一种聚合物构成的诱导剂保持体，用于保持对微生物相邻的表现生物作用的诱导物。土壤修复方法包括将微生物保持载体施加到土壤中。土壤补救剂包括微生物保持载体和其上持有的微生物，其表现出在载体中释放的诱导剂作用下在土壤中分解污染物质的酶活性。

4. 发明授权

US5807736A Method for biodegradation of aromatic and chlorinated compounds using microorganism strain FERM BP-5102 失效
标题翻译：使用微生物菌株FERM BP-5102生物降解芳香族和氯化化合物的方法
公开(公告)号：US5807736A
公开(公告)日：1998-09-15
申请号：US454515
申请日：1995-05-30
申请人： Shinya Kozaki , Kinya Kato , Tetsuya Yano , Takeshi Imamura
发明人： Shinya Kozaki , Kinya Kato , Tetsuya Yano , Takeshi Imamura
IPC分类号： A62D3/00 , A62D3/02 , B09C1/10 , C02F3/34 , C02F11/02 , C12N1/20 , C12R1/15 , C02F3/00 , C12N1/00 , D06M16/00
CPC分类号： C12R1/15 , C02F3/34 , Y10S435/821 , Y10S435/822 , Y10S435/843
摘要： A bacterial strain J1 (FERM BP-5102) which can effectively degrade aromatic compounds and/or chlorinated organic compounds such as trichloroethylene (TCE) is disclosed. Also the degradation occurs at a lower temperature such as 15.degree.. Further, a method for purifying waste water, soil or a gas polluted with the above chemical compounds utilizing the bacterium is disclosed.
摘要翻译：公开了能够有效降解芳族化合物和/或氯化有机化合物如三氯乙烯（TCE）的细菌菌株J1（FERM BP-5102）。此外，降解发生在较低温度如15℃。此外，公开了利用细菌净化上述化合物污染的废水，土壤或气体的方法。

5. 发明授权

US5993658A Method for biodegrading trichloroethylene and method for biodegrading chlorinated organic compound by microorganisms 失效
标题翻译：生物降解三氯乙烯的方法及微生物生物降解氯化有机化合物的方法
公开(公告)号：US5993658A
公开(公告)日：1999-11-30
申请号：US707497
申请日：1996-09-24
申请人： Kinya Kato , Shinya Kozaki , Takeshi Imamura , Toshiyuki Komatsu
发明人： Kinya Kato , Shinya Kozaki , Takeshi Imamura , Toshiyuki Komatsu
IPC分类号： A62D3/00 , A62D3/02 , B09C1/10 , C02F3/12 , C02F3/34 , C12N1/00 , C12N1/20 , C12P1/04 , C12R1/38 , C12S99/00 , C99Z99/00
CPC分类号： C02F3/1231 , B09C1/10 , C02F3/34 , C12P1/04 , C12R1/38 , Y02W10/15 , Y10S210/908 , Y10S210/909 , Y10S435/874
摘要： There are here provided a method for biodegrading trichloroethylene which comprises the step of bringing an aqueous medium containing trichloroethylene into contact with microorganisms having a trichloroethylene degrading ability derived from intestines of termites to degrade trichloroethylene; a method for obtaining the microorganisms; a method for remediating a soil by the use of the microorganisms; and a method for biodegrading an chlorinated organic compound with the microorganisms.
摘要翻译：这里提供了一种生物降解三氯乙烯的方法，其包括使含三氯乙烯的水性介质与来自白蚁肠的三氯乙烯降解能力的微生物接触以降解三氯乙烯的步骤; 获得微生物的方法; 一种通过使用微生物来补救土壤的方法; 以及用微生物生物降解氯化有机化合物的方法。

6. 发明授权

US07354995B2 Magnetic substance-biosubstance complex structure, peptide fragment capable of linking to magnetic substance and gene therefor, and process for producing the complex structure 失效
标题翻译：磁性物质 - 生物体复合结构，能够与磁性物质连接的肽片段及其基因，以及复合结构体的制造方法
公开(公告)号：US07354995B2
公开(公告)日：2008-04-08
申请号：US10546404
申请日：2004-04-30
申请人： Takeshi Imamura , Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Tsutomu Honma , Akiko Tsuchitani
发明人： Takeshi Imamura , Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Tsutomu Honma , Akiko Tsuchitani
IPC分类号： C07K7/08 , C07K17/14
CPC分类号： C12N9/1025 , C07K2319/00 , C12N11/06 , C12N11/14 , C12N15/1013 , G01N33/54326 , G01N33/54353 , Y10S530/811
摘要： A magnetic substance-biosubstance complex structure comprises a magnetic substance-containing carrier and a bio-substance immobilized on the carrier, the biosubstance being immobilized through a spacer comprising an amino acid sequence on a surface of the carrier.
摘要翻译：磁性物质 - 生物物质复合结构包括含磁性物质的载体和固定在载体上的生物物质，生物质通过载体表面上的氨基酸序列的间隔物固定化。

7. 发明申请

US20070054315A1 Magnetic substance-biosubstance complex structure, peptide fragment capable of linking to magnetic substance and gene therefor, and process for producing the complex structure 失效
标题翻译：磁性物质 - 生物体复合结构，能够与磁性物质连接的肽片段及其基因，以及复合结构体的制造方法
公开(公告)号：US20070054315A1
公开(公告)日：2007-03-08
申请号：US10546404
申请日：2004-04-30
申请人： Takeshi Imamura , Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Tsutomu Honma , Akiko Tsuchitani
发明人： Takeshi Imamura , Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Tsutomu Honma , Akiko Tsuchitani
IPC分类号： C40B40/10 , C40B40/04 , C07H21/04 , C12P21/06 , C12P7/62 , C12N9/18 , C12N15/74
CPC分类号： C12N9/1025 , C07K2319/00 , C12N11/06 , C12N11/14 , C12N15/1013 , G01N33/54326 , G01N33/54353 , Y10S530/811
摘要： A magnetic substance-biosubstance complex structure comprises a magnetic substance-containing carrier and a bio-substance immobilized on the carrier, the biosubstance being immobilized through a spacer comprising an amino acid sequence on a surface of the carrier.
摘要翻译：磁性物质 - 生物物质复合结构包括含磁性物质的载体和固定在载体上的生物物质，生物质通过载体表面上的氨基酸序列的间隔物固定化。

8. 发明授权

US07135540B2 Polyhydroxyalkanoate copolymer including unit having bromo group in side chain and method for producing the same 失效
标题翻译：聚羟基链烷酸酯共聚物，包括在侧链中具有溴基的单元及其制备方法
公开(公告)号：US07135540B2
公开(公告)日：2006-11-14
申请号：US10359600
申请日：2003-02-07
申请人： Tsutomu Honma , Shinya Kozaki , Takeshi Imamura , Takashi Kenmoku , Tatsuki Fukui , Etsuko Sugawa , Tetsuya Yano
发明人： Tsutomu Honma , Shinya Kozaki , Takeshi Imamura , Takashi Kenmoku , Tatsuki Fukui , Etsuko Sugawa , Tetsuya Yano
IPC分类号： C08G63/02
CPC分类号： C12P7/625 , C08G63/6822 , C08G63/6882 , C12P11/00
摘要： The invention provides a polyhydroxyalkanoate having a bromo group in a unit and being thermally stable and capable of arbitrarily controlling physical properties, and a producing method thereof. According to the invention, there are provided a polyhydroxyalkanoate copolymer including a 3-hydroxy-ω-bromoalkanoic acid unit represented by a formula—[OCH((CH2)nBr)CH2C(O)]— (n=1 to 8) (1) and a unit represented by a formula —[OCH((CH2)mR)CH2C(O)]— (2) within a same molecule, and a method for producing a polyhydroxyalkanoate copolymer by microorganisms, utilizing a ω-bromoalkanoic acid represented by a formula Br(CH2)pCH2CH2COOH (p=1 to 8) (20) and a compound represented by a formula R(CH2)qCH2CH2COOH (21) as raw materials.
摘要翻译：本发明提供一种具有溴基团并且是热稳定的并能够任意控制物理性能的聚羟基链烷酸酯及其制备方法。根据本发明，提供了一种包含由式 - [OCH（（CH 2）n）n Br表示的3-羟基-ω-溴代链烷酸单元的聚羟基链烷酸酯共聚物）CH 2 C（O）] - （n = 1-8）（1）和由式 - [OCH（（CH 2）2）（O）] - （2），以及通过微生物制备聚羟基链烷酸酯共聚物的方法，使用由以下化合物表示的ω-溴烷酸：式（Br 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 COOH（p = 1〜8）（20 ）和由式R（CH 2 CH 2）q CH 2 CH 2 COOH（21）表示的化合物作为原料。

9. 发明授权

US06660516B1 Method for culturing a microorganism and promoting microbial growth and metabolism 失效
标题翻译：培养微生物并促进微生物生长和代谢的方法
公开(公告)号：US06660516B1
公开(公告)日：2003-12-09
申请号：US09025424
申请日：1998-02-18
申请人： Takeshi Imamura , Yasutsugu Yamada , Tetsuya Yano , Akira Kuriyama , Shinya Kozaki
发明人： Takeshi Imamura , Yasutsugu Yamada , Tetsuya Yano , Akira Kuriyama , Shinya Kozaki
IPC分类号： B09B300
CPC分类号： C12N1/38 , C02F1/4618 , C02F3/34 , C02F2209/04 , C02F2209/06 , C12R1/19 , Y10S435/822 , Y10S435/849
摘要： A method is provided for culturing a microorganism in a culture medium containing a carbon source and an electrolyzed water containing not more than 0.4 ppm chlorine produced during electrolyzing water containing an electrolyte in an electrolytic cell. The culture medium may also contain a carbon source and an acidic water having a pH value of 1-4 and a redox potential from 800 mV to 1500 mV. The acidic water is obtained by electrolysis of water in an electrolytic cell. Further, the culture medium may contain a carbon source and an alkaline water having a pH value of 10-13 and a redox potential from −1000 mV to 800 mV. The alkaline water is obtained by electrolysis of water in an electrolytic cell. The redox potential for the alkaline water is determined by the use of a platinum electrode as a working electrode and a silver-silver chloride electrode as a reference electrode. The microorganism may be selected from Escherichia coli, strain J1, strain JM2N, or an artificial recombinant. The microorganism can decompose a pollutant, such as phenol, toluene, cresol, trichlorethylene and dichloroethylene.
摘要翻译：提供了一种在含有碳源的培养基中培养微生物的方法，以及在电解槽中含有电解质中含有电解质的水中产生的含有不超过0.4ppm氯的电解水。培养基也可以含有碳源和pH值为1-4，氧化还原电位为800mV〜1500mV的酸性水。酸性水通过在电解池中电解水而获得。此外，培养基可以含有碳源和pH值为10-13，氧化还原电位为-1000mV至800mV的碱性水。碱性水通过在电解槽中电解电解而获得。碱性水的氧化还原电位通过使用铂电极作为工作电极和银 - 氯化银电极作为参比电极来确定。微生物可以选自大肠杆菌，菌株J1，菌株JM2N，或人工重组体。微生物可以分解苯酚，甲苯，甲酚，三氯乙烯和二氯乙烯等污染物。

10. 发明授权

US07527809B2 Polyhydroxyalkanoate-containing magnetic structure, and manufacturing method and use thereof 失效
标题翻译：含聚羟基链烷酸酯的磁性结构体及其制造方法和用途
公开(公告)号：US07527809B2
公开(公告)日：2009-05-05
申请号：US10553221
申请日：2004-04-30
申请人： Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Takeshi Imamura , Tsutomu Honma
发明人： Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Takeshi Imamura , Tsutomu Honma
IPC分类号： A61K9/14 , A61K9/50
CPC分类号： A61K9/5094 , A61K49/226 , Y10T428/12014
摘要： A structure containing polyhydroxyalkanoate and a magnetic substance includes an external phase part containing polyhydroxyalkanoate and an internal phase part contained in the external phase part, at least one of the external phase part and the internal phase part containing a magnetic substance. Accordingly, provided is a microcapsule, which can be suitably used for formation of an active ingredient of a sustained release pharmaceutical preparation or an ultrasonic contrast agent.
摘要翻译：包含聚羟基链烷酸酯和磁性物质的结构包括含有聚羟基链烷酸酯的外相部分和包含在外部相部分中的内相部分，外相部分和内相部分中的至少一个含有磁性物质。因此，提供了一种微胶囊，其可适用于形成持续释放药物制剂或超声造影剂的活性成分。

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