专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20090215118A1 D-AMINOACYLASE 审中-公开
标题翻译： D-氨基酸
公开(公告)号：US20090215118A1
公开(公告)日：2009-08-27
申请号：US12397879
申请日：2009-03-04
申请人： Kimiyasu ISOBE , Seiki Yamaguchi , Masayuki Kobayashi , Shinya Kumagai , Takami Sarashina
发明人： Kimiyasu ISOBE , Seiki Yamaguchi , Masayuki Kobayashi , Shinya Kumagai , Takami Sarashina
IPC分类号： C12P21/00 , C12N9/48 , C07H21/00 , C12N15/74 , C12N1/21 , C12N1/20
CPC分类号： C12N9/80
摘要： A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost.A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.
摘要翻译：提供了具有高底物特异性的D-氨基酸酰化酶。该D-氨基酸酶可以以低成本方便且有效地从N-乙酰基-D，L-氨基酸产生D-氨基酸。由去纤维杆菌属的微生物产生的D-氨基酸酰化酶; 其作用于N-乙酰基-D-氨基酸; 其具有约55,000道尔顿的分子量（通过电泳测定）和5.3的等电点（通过用于变性体系的二维电泳测定）; 其作用于N-乙酰基-D-缬氨酸，N-乙酰基-D-亮氨酸等，但不适用于N-乙酰基-L-缬氨酸，N-乙酰基-L-亮氨酸等; 其最适温度为37℃（pH8），最适pH值为8〜8.5，在37℃下。 Mn2 +，Co2 +，Ni2 +和Zn2 +各自以1 mmol / L的速度抑制其活性，二硫苏糖醇，2-巯基乙醇，邻菲咯啉和L-半胱氨酸各自以5 mmol / L的速度进行抑制。

IPRDB

热门服务

关于我们

友情链接

联系方式