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    • 5. 发明授权
    • Method for chemical analysis of biological material
    • 生物材料化学分析方法
    • US06790669B1
    • 2004-09-14
    • US10018629
    • 2001-12-14
    • Curtis D. PfeifferNile N. FrawleyThomas L. PetersPhilip J. SavickasDavid R. AlbersSteven J. GluckLawrence W. NicholsonJose B. Esquivel H.
    • Curtis D. PfeifferNile N. FrawleyThomas L. PetersPhilip J. SavickasDavid R. AlbersSteven J. GluckLawrence W. NicholsonJose B. Esquivel H.
    • G01N3014
    • G01N30/88G01N30/78G01N2030/625G01N2030/8813Y10T436/143333Y10T436/25Y10T436/255
    • A chemical analysis method for determining chemically related differences between subject biological material such as genetically modified plant material and control biological material such as genetically unmodified plant material, which method includes at least the following six steps. The first step is to contact the subject biological material with a fluid extractant, such as a mixture of water, isopropanol and potassium hydroxide, to produce a fluid extract of the subject biological material. The second step is to contact the control biological material with the fluid extractant to produce a fluid extract of the control biological material. The third step is to chromatograph the fluid extract of the subject biological material, for example, gas or fluid chromatography, to produce a chromatogram of the fluid extract of the subject biological material. The fourth step is to chromatograph the fluid extract of the control biological material to produce a chromatogram of the fluid extract of the control biological material. The fifth step is to determine the differences between the chromatograms, for example, by using the method of U.S. Pat. No. 5,592,402, to identify at least one outlier peak. The sixth step is to determine the chemical identity of the outlier peak, for example, using gas chromatography/mass spectroscopy analysis of the outlier peak.
    • 一种化学分析方法,用于确定受试生物材料如转基因植物材料和对照生物材料如基因未修饰植物材料之间的化学相关差异,该方法至少包括以下六个步骤。 第一步是用流体萃取剂如水,异丙醇和氢氧化钾的混合物接触主题生物材料,以产生受试者生物材料的流体提取物。 第二步是将控制生物材料与流体萃取剂接触以产生对照生物材料的流体萃取物。 第三步是对目标生物材料的流体提取物进行色谱分离,例如气相色谱或流体色谱法,以产生该物质生物材料的液体提取物的色谱图。 第四步是对对照生物材料的流体提取物进行色谱分析以产生对照生物材料的液体提取物的色谱图。 第五步是确定色谱图之间的差异,例如,使用美国专利No. 确定至少一个异常值峰值的第5,592,402号。 第六步是确定离子峰的化学特性,例如使用离子峰的气相色谱/质谱分析。