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1. 发明公开

EP1181313A2 PEPTIDE ZUR VAKZINIERUNG GEGEN DAS HUMANE CMV 有权
标题翻译：肽疫苗接种反对人类巨细胞病毒
公开(公告)号：EP1181313A2
公开(公告)日：2002-02-27
申请号：EP00945603.9
申请日：2000-06-02
申请人： Kern, Florian
发明人： KERN, Florian , VOLK, Hans-Dieter , REINKE,Petra , FAULHABER, Nicole , SUREL, Ingolf-Pascal , KHATAMZAS, Elham
IPC分类号： C07K14/045 , A61K39/25 , C12N15/38 , C12N15/63 , G01N33/68
CPC分类号： C07K14/005 , A61K39/00 , C12N2710/16122 , C12N2710/16134 , Y02P20/55
摘要： The invention relates to peptides which are optionally fragments of the IE-1 or pp65 protein selected from the following group having sequence RN - Gln Thr Met Leu Arg Lys Glu Val Asn Ser Gln Leu Ser Leu Gly - RC; RN - Cys Asn Glu Asn Pro Glu Lys Asp Val Leu Ala Glu Leu Val Lys - RC; RN - Leu Val Lys Gln Ile Lys Val Arg Val Asp Met Val Arg His Arg - RC; RN - Ala Ala Asn Lys Leu Gly Gly Ala Leu Gln Ala Lys Ala Arg Ala - RC; RN - Ala Arg Ala Lys Lys Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Glu Leu Arg Arg Lys Met Met Tyr Met Cys Tyr Arg Asn Ile Glu - RC; RN - Val Thr Ser Asp Ala Cys Met Met Thr Met Tyr Gly Gly Ile Ser - RC; RN - Glu Phe Cys Arg Val Leu Cys Cys Tyr Val Leu Glu Glu Thr Ser - RC; RN - Met Ser Ile Tyr Val Tyr Ala Leu Pro Leu Lys Met Leu Asn Ile - RC; RN - Val Tyr Ala Leu Pro Leu Lys Met Leu Asn Ile Pro Ser Ile Asn - RC; RN - Ala Leu Pro Leu Lys Met Leu Asn Ile - RC; RN - His Ile Met Leu Asp Val Ala Phe Thr Ser His Glu His Phe Gly RC; RN - Asp Val Ala Phe Thr Ser His Glu His Phe Gly Leu Leu Cys Pro - RC; RN - Val Ala Phe Thr Ser His Glu His Phe - RC; RN - Ala Phe Thr Ser His Glu His Phe Gly - RC; RN - Ala Asn Asp Ile Tyr Arg Ile Phe Ala Glu Leu Glu Gly Val Trp - RC; RN - Val Cys Ser Met Glu Asn Thr Arg Ala Thr Lys Met Gln Val Ile - RC; RN - Glu Asn Thr Arg Ala Thr Lys Met Gln Val Ile Gly Asp Gln Tyr - RC; RN - Asn Thr Arg Ala Thr Lys Met Gln Val - RC; RN - Thr Arg Ala Thr Lys Met Gln Val Ile - RC; RN - Gln Pro Phe Met Arg Pro His Glu Arg Asn Gly Phe Thr Val Leu - RC; RN - Pro Leu Lys Met Leu Asn Ile Pro Ser Ile Asn Val His His Tyr - RC; RN - Leu Asn Ile Pro Ser Ile Asn Val His His Tyr Pro Ser Ala Ala - RC; RN Glu Asp Val Pro Ser Glu Lys Leu Phe Met His Val Thr Leu Gly - RC; RN - Cys Arg Val Leu Cys Cys Tyr Val Leu - RC; RN - Arg Val Leu Cys Cys Tyr Val Leu Glu - RC; RN - Val Leu Cys Cys Tyr Val Leu Glu Glu - RC; RN - Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Leu Arg Arg Lys Met Met Tyr - RC; RN Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Glu Glu Ala Ile Val Ala Tyr Tyr Leu Ala Thr Ala Gly - RC or RN - Glu Asn Ser Asp Gln Glu Ser Glu Gln Ser Asp Glu Glu Glu - RC, whereby RN represents -H or an amino protecting group; RC represents -OH or a carboxyl protecting group. The inventive peptides are used in the preparation of a medicaments for vaccination against HVMV infections or a diagnostic reagent for identifying an immune response against HCMV.

2. 发明公开

EP1257290A2 VERFAHREN ZUR ANTIGEN-SPEZIFISCHEN STIMULATION VON T-LYMPHOZYTEN MIT SYNTHETISCHEN PEPTIDBIBLIOTHEKEN 有权
标题翻译：方法用合成肽库的T淋巴细胞抗原特异性刺激
公开(公告)号：EP1257290A2
公开(公告)日：2002-11-20
申请号：EP01919295.4
申请日：2001-02-17
申请人： Kern, Florian
发明人： KERN, Florian , VOLK, Hans-Dieter , REINKE, Petra , FAULHABER, Nicole , SUREL, Ingolf-Pascal , KHATAMZAS, Elham
IPC分类号： A61K39/00 , A61K39/245 , A61K38/08 , A61K38/10 , A61K38/16 , C12N5/06 , C12N5/08 , G01N33/68 , G01N33/53
CPC分类号： G01N33/505
摘要： The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with synthetic peptide libraries, comprising the following steps: a) dividing the whole amino acid sequence of the antigen into protein fragments with partial amino acid sequences; b) synthesising a peptide library containing these protein fragments; c) incubating a suspension containing CD8- and/or CD4-T-lymphocytes with all of the protein fragments of the peptide library in a single culture batch. The method can be used for immunostimulation of T-lymphocytes of mammals, especially humans, as well as for diagnosis purposes, in order to determine whether a mammal, especially a human being, has previously responded to a specific protein with its/his/her immune system, and the strength of this response.

3. 发明公开

EP1051619A2 VERFAHREN ZUM IDENTIFIZIEREN VON T-ZELL-STIMULIERENDEN PROTEINFRAGMENTEN 无效
标题翻译：一种鉴定T细胞刺激蛋白片段
公开(公告)号：EP1051619A2
公开(公告)日：2000-11-15
申请号：EP99930888.5
申请日：1999-01-15
申请人： Kern, Florian
发明人： VOLK, Hans-Dieter , WALDEN, Peter , SCHEFFOLD, Alexander , BLASCZYK, Rainer , KERN, Florian
IPC分类号： G01N33/50 , G01N33/68 , C12P21/00 , A61K38/17
CPC分类号： G01N33/6863
摘要： The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen; b) subdividing the found amino acid sequence of the antigen into protein fragments; c) synthesizing at least one protein fragment; d) incubating a suspension containing t-cells with the protein fragments; e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and; f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.

4. 发明授权

EP1257290B2 VERFAHREN ZUR ANTIGEN-SPEZIFISCHEN STIMULATION VON T-LYMPHOZYTEN MIT SYNTHETISCHEN PEPTIDBIBLIOTHEKEN 有权
标题翻译：方法用合成肽库的T淋巴细胞抗原特异性刺激
公开(公告)号：EP1257290B2
公开(公告)日：2013-03-27
申请号：EP01919295.4
申请日：2001-02-17
申请人： Kern, Florian
发明人： KERN, Florian , VOLK, Hans-Dieter , REINKE, Petra , FAULHABER, Nicole , SUREL, Ingolf-Pascal , KHATAMZAS, Elham
IPC分类号： A61K39/00 , A61K39/245 , A61K38/08 , A61K38/10 , A61K38/16 , G01N33/68 , G01N33/53
CPC分类号： G01N33/505

5. 发明授权

EP1181313B1 PEPTIDE ZUR VAKZINIERUNG GEGEN DAS HUMANE CMV 有权
标题翻译：肽疫苗接种反对人类巨细胞病毒
公开(公告)号：EP1181313B1
公开(公告)日：2011-02-09
申请号：EP00945603.9
申请日：2000-06-02
申请人： Kern, Florian
发明人： KERN, Florian , VOLK, Hans-Dieter , REINKE,Petra , FAULHABER, Nicole , SUREL, Ingolf-Pascal , KHATAMZAS, Elham
IPC分类号： C07K14/045 , A61K39/25 , C12N15/38 , C12N15/63 , G01N33/68
CPC分类号： C07K14/005 , A61K39/00 , C12N2710/16122 , C12N2710/16134 , Y02P20/55
摘要： The invention relates to peptides which are optionally fragments of the IE-1 or pp65 protein selected from the following group having sequence RN - Gln Thr Met Leu Arg Lys Glu Val Asn Ser Gln Leu Ser Leu Gly - RC; RN - Cys Asn Glu Asn Pro Glu Lys Asp Val Leu Ala Glu Leu Val Lys - RC; RN - Leu Val Lys Gln Ile Lys Val Arg Val Asp Met Val Arg His Arg - RC; RN - Ala Ala Asn Lys Leu Gly Gly Ala Leu Gln Ala Lys Ala Arg Ala - RC; RN - Ala Arg Ala Lys Lys Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Glu Leu Arg Arg Lys Met Met Tyr Met Cys Tyr Arg Asn Ile Glu - RC; RN - Val Thr Ser Asp Ala Cys Met Met Thr Met Tyr Gly Gly Ile Ser - RC; RN - Glu Phe Cys Arg Val Leu Cys Cys Tyr Val Leu Glu Glu Thr Ser - RC; RN - Met Ser Ile Tyr Val Tyr Ala Leu Pro Leu Lys Met Leu Asn Ile - RC; RN - Val Tyr Ala Leu Pro Leu Lys Met Leu Asn Ile Pro Ser Ile Asn - RC; RN - Ala Leu Pro Leu Lys Met Leu Asn Ile - RC; RN - His Ile Met Leu Asp Val Ala Phe Thr Ser His Glu His Phe Gly RC; RN - Asp Val Ala Phe Thr Ser His Glu His Phe Gly Leu Leu Cys Pro - RC; RN - Val Ala Phe Thr Ser His Glu His Phe - RC; RN - Ala Phe Thr Ser His Glu His Phe Gly - RC; RN - Ala Asn Asp Ile Tyr Arg Ile Phe Ala Glu Leu Glu Gly Val Trp - RC; RN - Val Cys Ser Met Glu Asn Thr Arg Ala Thr Lys Met Gln Val Ile - RC; RN - Glu Asn Thr Arg Ala Thr Lys Met Gln Val Ile Gly Asp Gln Tyr - RC; RN - Asn Thr Arg Ala Thr Lys Met Gln Val - RC; RN - Thr Arg Ala Thr Lys Met Gln Val Ile - RC; RN - Gln Pro Phe Met Arg Pro His Glu Arg Asn Gly Phe Thr Val Leu - RC; RN - Pro Leu Lys Met Leu Asn Ile Pro Ser Ile Asn Val His His Tyr - RC; RN - Leu Asn Ile Pro Ser Ile Asn Val His His Tyr Pro Ser Ala Ala - RC; RN Glu Asp Val Pro Ser Glu Lys Leu Phe Met His Val Thr Leu Gly - RC; RN - Cys Arg Val Leu Cys Cys Tyr Val Leu - RC; RN - Arg Val Leu Cys Cys Tyr Val Leu Glu - RC; RN - Val Leu Cys Cys Tyr Val Leu Glu Glu - RC; RN - Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Leu Arg Arg Lys Met Met Tyr - RC; RN Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Glu Glu Ala Ile Val Ala Tyr Tyr Leu Ala Thr Ala Gly - RC or RN - Glu Asn Ser Asp Gln Glu Ser Glu Gln Ser Asp Glu Glu Glu - RC, whereby RN represents -H or an amino protecting group; RC represents -OH or a carboxyl protecting group. The inventive peptides are used in the preparation of a medicaments for vaccination against HVMV infections or a diagnostic reagent for identifying an immune response against HCMV.

6. 发明授权

EP1257290B1 VERFAHREN ZUR ANTIGEN-SPEZIFISCHEN STIMULATION VON T-LYMPHOZYTEN MIT SYNTHETISCHEN PEPTIDBIBLIOTHEKEN 有权
标题翻译：方法用合成肽库的T淋巴细胞抗原特异性刺激
公开(公告)号：EP1257290B1
公开(公告)日：2009-01-07
申请号：EP01919295.4
申请日：2001-02-17
申请人： Kern, Florian
发明人： KERN, Florian , VOLK, Hans-Dieter , REINKE, Petra , FAULHABER, Nicole , SUREL, Ingolf-Pascal , KHATAMZAS, Elham
IPC分类号： A61K39/00 , A61K39/245 , A61K38/08 , A61K38/10 , A61K38/16 , C12N5/06 , C12N5/08 , G01N33/68 , G01N33/53
CPC分类号： G01N33/505
摘要： The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with synthetic peptide libraries, comprising the following steps: a) dividing the whole amino acid sequence of the antigen into protein fragments with partial amino acid sequences; b) synthesising a peptide library containing these protein fragments; c) incubating a suspension containing CD8- and/or CD4-T-lymphocytes with all of the protein fragments of the peptide library in a single culture batch. The method can be used for immunostimulation of T-lymphocytes of mammals, especially humans, as well as for diagnosis purposes, in order to determine whether a mammal, especially a human being, has previously responded to a specific protein with its/his/her immune system, and the strength of this response.

7. 发明授权

EP1051619B1 VERFAHREN ZUM IDENTIFIZIEREN VON T-ZELL-STIMULIERENDEN PROTEINFRAGMENTEN 无效
标题翻译：一种鉴定T细胞刺激蛋白片段
公开(公告)号：EP1051619B1
公开(公告)日：2006-08-09
申请号：EP99930888.5
申请日：1999-01-15
申请人： Kern, Florian
发明人： VOLK, Hans-Dieter , WALDEN, Peter , SCHEFFOLD, Alexander , BLASCZYK, Rainer , KERN, Florian
IPC分类号： G01N33/50 , G01N33/68 , C12P21/00 , A61K38/17
CPC分类号： G01N33/6863
摘要： The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen; b) subdividing the found amino acid sequence of the antigen into protein fragments; c) synthesizing at least one protein fragment; d) incubating a suspension containing t-cells with the protein fragments; e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and; f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.

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