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1. 发明授权

US07283306B2 Confocal optical scanner 失效
标题翻译：共焦光学扫描仪
公开(公告)号：US07283306B2
公开(公告)日：2007-10-16
申请号：US10844421
申请日：2004-05-13
申请人： Kenta Mikuriya , Takeo Tanaami , Naoki Seki , Takeharu Nagai , Atsushi Miyawaki
发明人： Kenta Mikuriya , Takeo Tanaami , Naoki Seki , Takeharu Nagai , Atsushi Miyawaki
IPC分类号： G02B27/14 , G02B21/00 , G02B26/02
CPC分类号： G02B21/0024 , G02B21/0044 , G02B21/0076
摘要： A confocal optical scanner of Nipkow disk type for measuring a sample, in which each component of two or more color pigments, has a high reflection mirror for separating an excited light to be radiated onto the sample and a fluorescence emitted from the sample, wherein a reflectance of the high reflection mirror is from 80% to 100% in a measured wavelength region including at least an excited light wavelength region and a fluorescence wavelength region. Thereby, the confocal optical scanner capable of measuring a polychromatic fluorescent image at the same time is realized with the enhanced light receiving efficiency of fluorescence, using one high reflection mirror without using a plurality of dichroic mirrors by exchange.
摘要翻译：用于测量样品的Nipkow盘类型的共焦光学扫描仪，其中两种或更多种颜色颜料的每种组分具有用于分离待辐射到样品上的激发光和从样品发射的荧光的高反射镜，其中a 在一个测量的波长区域中，至少包括激发光波长区域和荧光波长区域，高反射镜的反射率为80％至100％。由此，能够通过使用一个高反射镜而不使用多个二向色镜通过交换来实现能够同时测量多色荧光图像的共焦光学扫描仪，其具有增强的荧光的光接收效率。

2. 发明授权

US07553624B2 Method of analyzing physiological function of target substance 失效
标题翻译：分析目标物质生理功能的方法
公开(公告)号：US07553624B2
公开(公告)日：2009-06-30
申请号：US10557906
申请日：2004-05-20
申请人： Takeharu Nagai , Atsushi Miyawaki
发明人： Takeharu Nagai , Atsushi Miyawaki
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/53 , C07H19/04 , C07H21/02
CPC分类号： G01N33/533
摘要： The present invention provides a method of analyzing the physiological function of a target substance by inactivating the physiological function of the target substance, comprising the steps of: (a) binding to the target substance a photoactive compound represented by formula (I): (in the formula, Q is a group for binding this compound with the target substance) to form a composite comprising the target substance and the photoactive compound; and (b) irradiating the obtained composite with light to inactivate the function of the target substance to which the photoactive compound has been bound, or to inactivate the function of the target substance at the site where the photoactive compound has been bound.
摘要翻译：本发明提供了通过使目标物质的生理功能失活来分析目标物质的生理功能的方法，包括以下步骤：（a）与目标物质结合由式（I）表示的光活性化合物：（在式中，Q为与化合物与目标物质结合的基团），形成包含目标物质和光活性化合物的复合体; 和（b）用光照射得到的复合物，使光活性化合物结合的目标物质的功能失活，或者使光活性化合物结合的位置失活目标物质的功能。

3. 发明申请

US20060275822A1 Fluorescent indicator using fret 审中-公开
标题翻译：荧光灯指示灯使用
公开(公告)号：US20060275822A1
公开(公告)日：2006-12-07
申请号：US10538772
申请日：2003-12-10
申请人： Atsushi Miyawaki , Takeharu Nagai
发明人： Atsushi Miyawaki , Takeharu Nagai
IPC分类号： G01N33/53 , C12Q1/37 , C07H21/04 , C12P21/06 , C07K14/435
CPC分类号： G01N33/542 , C07K14/43595 , C07K2319/00 , C07K2319/05 , C07K2319/06 , C07K2319/07 , C07K2319/09
摘要： It is an object of the present invention to provide a fluorescent indicator that is based on homo FRET (fluorescence resonance energy transfer) using fluorescent molecules with a small stokes' shift, thereby developing a visualization system for intermolecular interaction. The present invention provides a fluorescent indicator formed by fusing fluorescent molecular components having substantially identical fluorescent properties to the N- and C-terminal sides of a target sequence, to which an analytical substance binds or reacts, so as to change the three-dimensional structure of the indicator.
摘要翻译：本发明的一个目的是提供一种基于使用具有小斯托克斯位移的荧光分子的同质FRET（荧光共振能量转移）的荧光指示剂，从而开发用于分子间相互作用的可视化系统。本发明提供了一种荧光指示剂，其通过将具有基本相同荧光性质的荧光分子组分与目标序列的N-末端和末端侧融合而形成，分析物质结合或反应，从而改变三维结构的指标。

4. 发明授权

US07981658B2 Nucleic acid molecules, vectors, and host cells encoding fluorescent protein 有权
标题翻译：核酸分子，载体和编码荧光蛋白的宿主细胞
公开(公告)号：US07981658B2
公开(公告)日：2011-07-19
申请号：US12569464
申请日：2009-09-29
申请人： Atsushi Miyawaki , Takeharu Nagai
发明人： Atsushi Miyawaki , Takeharu Nagai
IPC分类号： C12N1/20 , C12N15/00 , C07K1/00 , C07H21/04
CPC分类号： C07K14/43595 , C07K2319/00
摘要： The present invention provides a fluorescent protein having an amino acid sequence of the green fluorescent protein, the yellow fluorescent protein or mutants thereof wherein the 46th phenylalanine residue is substituted with a leucine residue. According to the present invention, there are provided novel GFP or YEP mutants having an excellent maturation efficacy and having a decreased sensitivity to both of H+ and CI31.
摘要翻译：本发明提供了具有绿色荧光蛋白的氨基酸序列，黄色荧光蛋白或其突变体的荧光蛋白，其中第46位苯丙氨酸残基被亮氨酸残基取代。根据本发明，提供了具有优异成熟功效并且对H +和CI31都具有降低的敏感性的新型GFP或YEP突变体。

5. 发明授权

US08013119B2 Fluorescent protein 失效
标题翻译：荧光蛋白
公开(公告)号：US08013119B2
公开(公告)日：2011-09-06
申请号：US11719166
申请日：2005-11-14
申请人： Takeharu Nagai , Atsushi Miyawaki
发明人： Takeharu Nagai , Atsushi Miyawaki
IPC分类号： C07K14/00
CPC分类号： G01N33/5035 , C07K14/43595 , G01N33/542
摘要： It is an object of the present invention to provide a fluorescent protein, which allows an acceptor for fluorescence resonance energy transfer (FRET) to appear in a stimulating light-dependent manner, thereby enabling the marking of any given cell organelle, cells, or tissues, with multiple colors. The present invention provides a fluorescent protein which consists of a fused protein of a donor fluorescent protein and an acceptor fluorescent protein, wherein before irradiation with stimulating light, the donor protein is able to emit fluorescence as a result of irradiation of the donor protein with excitation light; and after irradiation with stimulating light, intramolecular FRET occurs between the donor fluorescent protein and the acceptor fluorescent protein as a result of irradiation of the donor protein with excitation light, and the acceptor protein is able to emit fluorescence, and wherein the fluorescence of the donor protein and the fluorescence of the acceptor protein have wavelengths that are different from each other.
摘要翻译：本发明的目的是提供一种荧光蛋白，其允许荧光共振能量转移受体（FRET）以刺激光依赖的方式出现，从而能够标记任何给定的细胞器，细胞或组织，具有多种颜色。本发明提供了由供体荧光蛋白的融合蛋白和受体荧光蛋白组成的荧光蛋白，其中在用刺激光照射之前，供体蛋白能够通过激发照射供体蛋白而发出荧光光; 并且在用刺激光照射后，作为供体蛋白质与激发光照射的结果，在供体荧光蛋白和受体荧光蛋白之间发生分子内FRET，并且受体蛋白能够发射荧光，并且其中供体的荧光蛋白质，受体蛋白质的荧光具有彼此不同的波长。

6. 发明授权

US07595375B2 Fluorescent protein 有权
标题翻译：荧光蛋白
公开(公告)号：US07595375B2
公开(公告)日：2009-09-29
申请号：US10162593
申请日：2002-06-06
申请人： Atsushi Miyawaki , Takeharu Nagai
发明人： Atsushi Miyawaki , Takeharu Nagai
IPC分类号： C07K14/00 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C07K14/43595 , C07K2319/00
摘要： The present invention provides a fluorescent protein having an amino acid sequence of the green fluorescent protein, the yellow fluorescent protein or mutants thereof wherein the 46th phenylalanine residue is substituted with a leucine residue. According to the present invention, there are provided novel GFP or YEP mutants having an excellent maturation efficacy and having a decreased sensitivity to both of H+ and Cl−.
摘要翻译：本发明提供了具有绿色荧光蛋白的氨基酸序列，黄色荧光蛋白或其突变体的荧光蛋白，其中第46位苯丙氨酸残基被亮氨酸残基取代。根据本发明，提供了具有优异成熟功效并且对H +和Cl-两者具有降低的敏感性的新型GFP或YEP突变体。

7. 发明申请

US20090176211A1 FLUORESCENT PROTEIN 失效
标题翻译：荧光蛋白
公开(公告)号：US20090176211A1
公开(公告)日：2009-07-09
申请号：US11719166
申请日：2005-11-14
申请人： Takeharu Nagai , Atsushi Miyawaki
发明人： Takeharu Nagai , Atsushi Miyawaki
IPC分类号： C12Q1/68 , C07K14/00 , C12N15/11 , C12N15/00 , C12N5/06
CPC分类号： G01N33/5035 , C07K14/43595 , G01N33/542
摘要： It is an object of the present invention to provide a fluorescent protein, which allows an acceptor for fluorescence resonance energy transfer (FRET) to appear in a stimulating light-dependent manner, thereby enabling the marking of any given cell organelle, cells, or tissues, with multiple colors. The present invention provides a fluorescent protein which consists of a fused protein of a donor fluorescent protein and an acceptor fluorescent protein, wherein before irradiation with stimulating light, the donor protein is able to emit fluorescence as a result of irradiation of the donor protein with excitation light; and after irradiation with stimulating light, intramolecular FRET occurs between the donor fluorescent protein and the acceptor fluorescent protein as a result of irradiation of the donor protein with excitation light, and the acceptor protein is able to emit fluorescence, and wherein the fluorescence of the donor protein and the fluorescence of the acceptor protein have wavelengths that are different from each other.
摘要翻译：本发明的目的是提供一种荧光蛋白，其允许荧光共振能量转移受体（FRET）以刺激光依赖的方式出现，从而能够标记任何给定的细胞器，细胞或组织，具有多种颜色。本发明提供了由供体荧光蛋白的融合蛋白和受体荧光蛋白组成的荧光蛋白，其中在用刺激光照射之前，供体蛋白能够通过激发照射供体蛋白而发出荧光光; 并且在用刺激光照射后，作为供体蛋白质与激发光照射的结果，在供体荧光蛋白和受体荧光蛋白之间发生分子内FRET，并且受体蛋白能够发射荧光，并且其中供体的荧光蛋白质，受体蛋白质的荧光具有彼此不同的波长。

8. 发明申请

US20090017516A1 TARGET PHYSIOLOGICAL FUNCTION INACTIVATOR USING PHOTOSENSITIZER-LABELED FLUORESCENT PROTEIN 审中-公开
标题翻译：使用光敏剂标记荧光蛋白的目标生理功能失活剂
公开(公告)号：US20090017516A1
公开(公告)日：2009-01-15
申请号：US11722283
申请日：2005-12-21
申请人： Takeharu Nagai , Atsushi Miyawaki
发明人： Takeharu Nagai , Atsushi Miyawaki
IPC分类号： C12N13/00 , C07K14/00 , C07F19/00 , C07K1/00
CPC分类号： C07K14/43595 , A61K41/0057 , A61K41/0071
摘要： An object of the present invention is to provide a method of generating reactive oxygen species in a light irradiation-dependent manner, so as to inactivate any target physiological function. The present invention provides a target physiological function inactivator which consists of a photosensitizer-labeled fluorescent protein, wherein fluorescence resonance energy transfer (FRET) from the fluorescent protein to the photosensitizer occurs as a result of light irradiation, so that the photosensitizer can be excited to generate reactive oxygen species.
摘要翻译：本发明的目的是提供一种依照光依赖的方式产生活性氧的方法，从而使任何目标生理功能失活。本发明提供了由光敏剂标记的荧光蛋白组成的目标生理功能失活剂，其中由于光照射而发生从荧光蛋白到光敏剂的荧光共振能量转移（FRET），使得光敏剂可被激发产生活性氧。

9. 发明申请

US20070161636A1 Method of analyzing physiological function of target substance 失效
标题翻译：分析目标物质生理功能的方法
公开(公告)号：US20070161636A1
公开(公告)日：2007-07-12
申请号：US10557906
申请日：2004-05-20
申请人： Takeharu Nagai , Atsushi Miyawaki
发明人： Takeharu Nagai , Atsushi Miyawaki
IPC分类号： A61K31/53 , A61K31/353 , C12Q1/68 , G01N33/53 , C07D311/82 , C07D311/88 , C07D405/02
CPC分类号： G01N33/533
摘要： The present invention provides a method of analyzing the physiological function of a target substance by inactivating the physiological function of the target substance, comprising the steps of: (a) binding to the target substance a photoactive compound represented by formula (I): (in the formula, Q is a group for binding this compound with the target substance) to form a composite comprising the target substance and the photoactive compound; and (b) irradiating the obtained composite with light to inactivate the function of the target substance to which the photoactive compound has been bound, or to inactivate the function of the target substance at the site where the photoactive compound has been bound.
摘要翻译：本发明提供了通过使目标物质的生理功能失活来分析目标物质的生理功能的方法，包括以下步骤：（a）与目标物质结合式（I）表示的光活性化合物：（在式中，Q为与化合物与目标物质结合的基团），形成包含目标物质和光活性化合物的复合体; 和（b）用光照射得到的复合物，使光活性化合物结合的目标物质的功能失活，或者使光活性化合物结合的位置失活目标物质的功能。

10. 发明申请

US20050208624A1 Fluorescent protein 审中-公开
标题翻译：荧光蛋白
公开(公告)号：US20050208624A1
公开(公告)日：2005-09-22
申请号：US11042158
申请日：2005-01-26
申请人： Atsushi Miyawaki , Takeharu Nagai
发明人： Atsushi Miyawaki , Takeharu Nagai
IPC分类号： G01N33/52 , C07K14/00 , C07K14/435 , C07K14/47 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12Q1/02 , G01N21/78 , G01N33/533 , G01N33/58 , G01N33/84 , G01N33/20 , C07H21/04 , C12Q1/68
CPC分类号： G01N33/533 , C07K14/43595 , C07K14/4728 , C07K2319/00 , G01N33/582 , G01N33/84
摘要： It is an object of the present invention to provide a novel fluorescent protein having sensitivity to a calcium ion, in particular a fluorescent protein which can be used as an indicator for quantifying calcium ion concentration. The present invention provides a fluorescent protein having the following amino acid sequences (1) to (3) in order in the direction from the N-terminus to the C-terminus, wherein a fused fluorescent protein obtained by fusion of the fluorescent protein with a calcium binding protein and its target peptide can emit fluorescence which is dependent on Ca2+ ion level; (1) an amino acid sequence from the nth amino acid from the N-terminus to the C-terminus of a fluorescent protein selected from the group consisting of a green fluorescent protein or its mutant, a yellow fluorescent protein or its mutant, a cyan fluorescent protein or its mutant, a red fluorescent protein or its mutant, and a blue fluorescent protein or its mutant, provided that n represents an integer of 140 to 150; (2) a linker sequence of a sequence of 2 to 20 amino acids; and (3) an amino acid sequence from the 1st amino acid to the (n-1)th amino acid from the N-terminus of the fluorescent protein described in (1) above.
摘要翻译：本发明的目的是提供一种对钙离子具有敏感性的新型荧光蛋白，特别是荧光蛋白，其可以用作定量钙离子浓度的指标。本发明提供了从N末端到C末端的顺序依次具有以下氨基酸序列（1）〜（3）的荧光蛋白质，其中通过荧光蛋白质与钙结合蛋白及其靶肽可以发射依赖于Ca 2+离子水平的荧光; （1）选自绿色荧光蛋白或其突变体，黄色的荧光蛋白的N末端至C末端的第n个氨基酸的氨基酸序列荧光蛋白或其突变体，青色荧光蛋白或其突变体，红色荧光蛋白或其突变体，以及蓝色荧光蛋白或其突变体，条件是n表示140至150的整数; （2）2至20个氨基酸序列的接头序列; 和（3）从第（1）个氨基酸至（n-1）第（N）个氨基酸的氨基酸序列，从（ 1）以上。

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