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    • 7. 发明申请
    • Methods for DNA recovery
    • DNA回收方法
    • US20050260641A1
    • 2005-11-24
    • US11090844
    • 2005-03-25
    • Katsuhisa Suzuki
    • Katsuhisa Suzuki
    • C12N15/09C12Q1/68C12P19/34
    • C12Q1/6855C12Q2525/191
    • It is intended to provide a method for DNA recovery capable of more reliably recovering a cDNA fragment of interest from a group of cDNA fragments in which various kinds of cDNA fragments are present. The method for DNA recovery comprises a first PCR step for carrying out a PCR reaction on the group of cDNA fragments each having a first adaptor sequence on one end and a second adaptor sequence on the other end, using a first primer having a sequence complementary to the first adaptor sequence and also having a labeling substance and a second primer having a sequence complementary to the second adaptor sequence. Also, an electrophoretic step for carrying out a gel electrophoresis on the group of cDNA fragments amplified in the first PCR step and, on the basis of a result of the electrophoretic step, a recovery step for cutting out a gel portion containing a cDNA fragment of interest from a gel and then recovering the cDNA fragment from the gel are comprised.
    • 旨在提供一种能够更可靠地从存在各种cDNA片段的cDNA片段组中更可靠地回收目的cDNA片段的DNA回收方法。 用于DNA回收的方法包括第一PCR步骤,用于对具有一端的第一衔接子序列的cDNA片段组和另一端的第二衔接子序列进行PCR反应,使用具有与 第一衔接子序列并且还具有标记物质和具有与第二衔接子序列互补的序列的第二引物。 另外,在第一PCR步骤中扩增的cDNA片段组上进行凝胶电泳的电泳步骤,并且基于电泳步骤的结果,进行用于切出含有cDNA片段的凝胶部分的回收步骤 包含凝胶的兴趣,然后从凝胶中回收cDNA片段。