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1. 发明授权

US06406898B1 D-pantolactone hydrolase and gene encoding the same 失效
标题翻译： D-泛酸内酯水解酶和编码基因的基因
公开(公告)号：US06406898B1
公开(公告)日：2002-06-18
申请号：US08836047
申请日：1997-09-19
申请人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu , Michihiko Kobayashi
发明人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu , Michihiko Kobayashi
IPC分类号： C12N914
CPC分类号： C12N9/18 , C12P17/04 , C12P41/001 , C12P41/005
摘要： A novel enzyme which is useful in the optical resolution of D,L-pantolactone via D-selective asymmetric hydrolysis and a gene encoding the the same are provided. The invention discloses the gene coding for a natural D-pantolactone hydrolase (for example, one originating in Fusarium oxysporum) or proteins having an activity substantially equivalent thereto; host cells transformed with DNA containing a nucleotide sequence coding for said protein, processes for producing said protein via using said host cells and uses of said proteins and host cells.
摘要翻译：提供了可用于通过D-选择性不对称水解的D，L-泛酸内酯的光学拆分和编码其的基因的新型酶。本发明公开了编码天然D-泛酸内酯水解酶（例如源于尖孢镰刀菌的一种）的基因或具有与其基本相当的活性的蛋白质; 用含有编码所述蛋白质的核苷酸序列的DNA转化的宿主细胞，通过使用所述宿主细胞产生所述蛋白质的过程和所述蛋白质和宿主细胞的用途。

2. 发明授权

US06794171B2 D-pantolactone hydrolase and gene encoding the same 失效
标题翻译： D-泛酸内酯水解酶和编码基因的基因
公开(公告)号：US06794171B2
公开(公告)日：2004-09-21
申请号：US10133372
申请日：2002-04-29
申请人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu , Michihiko Kobayashi
发明人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu , Michihiko Kobayashi
IPC分类号： C12N914
CPC分类号： C12N9/18 , C12P17/04 , C12P41/001 , C12P41/005
摘要： A novel enzyme which is useful in the optical resolution of D,L-pantolactone via D-selective asymmetric hydrolysis and a gene encoding the the same are provided. The invention discloses the gene coding for a natural D-pantolactone hydrolase (for example, one originating in Fusarium oxysporum) or proteins having an activity substantially equivalent thereto; host cells transformed with DNA containing a nucleotide sequence coding for said protein, processes for producing said protein via using said host cells and uses of said proteins and host cells.
摘要翻译：提供了可用于通过D-选择性不对称水解的D，L-泛酸内酯的光学拆分和编码其的基因的新型酶。本发明公开了编码天然D-泛酸内酯水解酶（例如源于尖孢镰刀菌的一种）的基因或具有与其基本相当的活性的蛋白质; 用含有编码所述蛋白质的核苷酸序列的DNA转化的宿主细胞，通过使用所述宿主细胞产生所述蛋白质的过程和所述蛋白质和宿主细胞的用途。

3. 发明授权

US5275949A Process for the preparation of D-pantolactone 失效
标题翻译：制备D-泛酸内酯的方法
公开(公告)号：US5275949A
公开(公告)日：1994-01-04
申请号：US671799
申请日：1991-04-01
申请人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu
发明人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu
IPC分类号： C12P7/42 , C12P41/00 , C12R1/645 , C12R1/665 , C12R1/77 , C12R1/82 , C12R1/845 , C12P7/40
CPC分类号： C12P7/42 , C12P41/003
摘要： A process for the preparation of D-pantolactone, comprising employing specific microorganisms to convert D,L-pantolactone as the starting material into D-pantoic acid by selective asymmetric hydrolysis of the D-form only in the D,L-pantolactone, and then separating D-pantoic acid and converting it into D-pantolactone.
摘要翻译： PCT No.PCT / JP90 / 00960 Sec。 371日期1991年4月1日 102（e）1991年4月1日PCT PCT 1990年7月27日PCT公布。出版物WO91 / 02081 1991年2月21日。一种制备D-泛酸内酯的方法，包括使用特异性微生物将D，L-泛酸内酯作为原料转化成D-泛酸，通过仅在D中的D型的选择性不对称水解，L-泛酸内酯，然后分离D-泛解酸并将其转化为D-泛酸内酯。

4. 发明授权

US5372940A D-pantolactone hydrolase and process for the preparation thereof 失效
标题翻译： D-泛酸内酯水解酶及其制备方法
公开(公告)号：US5372940A
公开(公告)日：1994-12-13
申请号：US859439
申请日：1992-06-02
申请人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu
发明人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu
IPC分类号： C12N9/18 , C12P7/42 , C12P17/04 , C12P41/00 , C12R1/645 , C12R1/665 , C12R1/77 , C12R1/82 , C12R1/845 , C12P17/02 , C12N9/14
CPC分类号： C12P7/42 , C12N9/18 , C12P17/04 , C12P41/005
摘要： The invention is drawn to a new enzyme that selectively hydrolyzes D-pantolactone in D,L-pantolactone and has the following characteristics:(a) action: acts on pantolactone to produce the corresponding acid;(b) specificity for substrate: acts specifically on D-pantolactone but not on L-pantolactone;(c) pH stability: stable at pH5-9;(d) optimal pH: 7.0-7.5;(e) optimal temperature: ca. 50.degree. C.; and(f) effect of various metal ions or inhibitors: inhibited by Cd.sup.2+, Hg.sup.2+ or Cu.sup.2+ EDTA, as well as a process for the preparation thereof. The enzyme is preferably produced by cultivating the microorganisms of the genera Fusarium, Cylindrocarpon, and Gibberella, and most preferably the microorganism Fusarium oxysporum IFO 5942.
摘要翻译： PCT No.PCT / JP91 / 01351 Sec。 371日期：1992年6月2日 102（e）日期1992年6月2日PCT提交1991年10月4日PCT公布。公开号WO92 / 06182 日期：1992年4月16日。本发明涉及一种在D，L-泛酸内酯中选择性水解D-泛酸内酯的新酶，具有以下特征：（a）作用：作用于泛酸内酯以产生相应的酸; （b）底物的特异性：特异性地对D-泛酸内酯作用，但不对L-泛酸内酯作用; （c）pH稳定性：pH5-9时稳定; （d）最佳pH：7.0-7.5; （e）最佳温度：约 50℃。和（f）各种金属离子或抑制剂的作用：被Cd2 +，Hg2 +或Cu2 + EDTA抑制，以及其制备方法。该酶优选通过栽培镰刀菌属，圆锥菌属和赤霉属的微生物，最优选微生物尖孢镰刀菌IFO 5942来产生。

5. 发明申请

US20070134775A1 Process for producing lactonase and utilization thereof 审中-公开
标题翻译：生产内酯酶的方法及其利用
公开(公告)号：US20070134775A1
公开(公告)日：2007-06-14
申请号：US10547424
申请日：2004-03-03
申请人： Sakayu Shimizu , Michihiko Kataoka , Keiji Sakamoto
发明人： Sakayu Shimizu , Michihiko Kataoka , Keiji Sakamoto
IPC分类号： C12P17/06 , C12N9/16 , C12N1/18
CPC分类号： C12P41/003 , C12N9/18 , C12P17/04
摘要： It has been required to economically produce a large amount of optically active γ-lactone derivatives (for example, pantolactone), which are useful as intermediates in synthesizing useful substances such as pharmaceutical drugs. To achieve this object, it is advantageous to employ an enzymatic technique of asymmetric hydrolysis with a hydrolyzing enzyme lactonase. However, it remains troublesome to prepare the enzyme or utilize a microorganism capable of producing the enzyme. Also, it is difficult to obtain a sufficient and stable enzymatic activity in the case of using recombination techniques. In producing lactonase, which asymmetrically hydrolyzes a γ-lactone derivative such as racemic pantolactone selectively, by a recombination technique, both mature lactonase DNA and signal peptide region DNA are transferred into a host. Thus, a stable lactonase activity comparable to naturally-occurring one can be achieved and a transformant sustaining a high enzyme activity in a stable manner can be acquired, thereby allowing the efficient and industrially advantageous asymmetric synthesis of γ-lactone derivatives.
摘要翻译：需要经济地生产大量的用作合成有用物质如药物的中间体的光学活性γ-内酯衍生物（例如泛酸内酯）。为了达到这个目的，使用水解酶内酯酶的不对称水解酶技术是有利的。然而，制备酶或利用能够产生酶的微生物仍然是麻烦的。此外，在使用重组技术的情况下，难以获得足够稳定的酶活性。在制备内含子选择性地水解γ-内酯衍生物如外消旋泛酸内酯的内切酶中，通过重组技术，将成熟内含酶DNA和信号肽区DNA两者转移到宿主中。因此，可以获得与天然存在的活性相当的稳定的内含酶活性，并且可以获得以稳定的方式维持高酶活性的转化体，从而允许有效和工业上有利的γ-内酯衍生物的不对称合成。

6. 发明授权

US06835559B2 Process for the production of optically active &bgr;-amino alcohols 失效
标题翻译：光学活性β-氨基醇的制备方法
公开(公告)号：US06835559B2
公开(公告)日：2004-12-28
申请号：US10240056
申请日：2003-01-22
申请人： Keiji Sakamoto , Shinji Kita , Kazuya Tsuzaki , Tadanori Morikawa , Sakayu Shimizu , Michihiko Kataoka
发明人： Keiji Sakamoto , Shinji Kita , Kazuya Tsuzaki , Tadanori Morikawa , Sakayu Shimizu , Michihiko Kataoka
IPC分类号： C12P1300
CPC分类号： C12P13/001 , C12P13/008 , C12P41/002 , Y10S435/822 , Y10S435/865 , Y10S435/877 , Y10S435/911 , Y10S435/914 , Y10S435/917 , Y10S435/918
摘要： A process for producing an optical active &bgr;-amino alcohol, the method comprising the step of allowing at least one microorganism selected from the group consisting of microorganisms belonging to the genus Morganella and others, to act on an enantiomeric mixture of an &agr;-aminoketone or a salt thereof having the general formula (I): to produce an optical active &bgr;-amino alcohol with the desired optical activity having the general formula (II) described below in a high yield as well as in a highly selective manner:
摘要翻译：一种制备光学活性β-氨基醇的方法，所述方法包括允许至少一种选自属于摩根氏菌属和其他微生物的微生物的微生物作用于α-氨基酮或其对映异构体混合物其具有通式（I）的盐：以高产率和高度选择性的方式制备具有下述通式（II）的具有所需光学活性的光学活性β-氨基醇：

7. 发明授权

US07166452B2 Aminoketone asymmetric reductase and nucleic acid thereof 有权
标题翻译：氨基酮不对称还原酶及其核酸
公开(公告)号：US07166452B2
公开(公告)日：2007-01-23
申请号：US10469682
申请日：2002-03-01
申请人： Keiji Sakamoto , Shinji Kita , Kazuya Tsuzaki , Tadanori Morikawa , Sakayu Shimizu , Michihiko Kataoka
发明人： Keiji Sakamoto , Shinji Kita , Kazuya Tsuzaki , Tadanori Morikawa , Sakayu Shimizu , Michihiko Kataoka
IPC分类号： C12N9/02 , C12P13/00 , C12P1/06 , C12P1/04
CPC分类号： C12N9/0006 , C12P13/001 , C12P13/008
摘要： A protein, which is an aminoketone asymmetric reductase, having an effect of producing d-pseudoephedrine by acting on 1-2-methylaminopropiophenone, and having the following physiochemical properties: substrate: 1-2-methylaminopropiophenone optimum pH: pH 8.1 optimum temperature: 55° C. coenzyme: NADP molecular weight: about 28500 Da homotetramer and a nucleic acid coding the protein.
摘要翻译：作为氨基酮不对称还原酶的蛋白质，具有通过作用于1-2-甲基氨基苯丙酮而产生d-伪麻黄碱的作用，具有以下物理化学性质：底物：1-2-甲基氨基苯丙酮最适pH：pH 8.1最适温度：55 ℃。辅酶：NADP分子量：约28500Da同型四聚体和编码该蛋白质的核酸。

8. 发明申请

US20090137012A1 Process for producing lactonase and utilitzation thereof 审中-公开
标题翻译：生产内酯酶的方法及其功效
公开(公告)号：US20090137012A1
公开(公告)日：2009-05-28
申请号：US12232870
申请日：2008-09-25
申请人： Sakayu Shimizu , Michihiko Kataoka , Keiji Sakamoto
发明人： Sakayu Shimizu , Michihiko Kataoka , Keiji Sakamoto
IPC分类号： C12P17/04
CPC分类号： C12P41/003 , C12N9/18 , C12P17/04
摘要： It has been required to economically produce a large amount of optically active γ-lactone derivatives (for example, pantolactone), which are useful as intermediates in synthesizing useful substances such as pharmaceutical drugs. To achieve this object, it is advantageous to employ an enzymatic technique of asymmetric hydrolysis with a hydrolyzing enzyme lactonase. However, it remains troublesome to prepare the enzyme or utilize a microorganism capable of producing the enzyme. Also, it is difficult to obtain a sufficient and stable enzymatic activity in the case of using recombination techniques. In producing lactonase, which asymmetrically hydrolyzes a γ-lactone derivative such as racemic pantolactone selectively, by a recombination technique, both mature lactonase DNA and signal peptide region DNA are transferred into a host. Thus, a stable lactonase activity comparable to naturally-occurring one can be achieved and a transformant sustaining a high enzyme activity in a stable manner can be acquired, thereby allowing the efficient and industrially advantageous asymmetric synthesis of γ-lactone derivatives.
摘要翻译：需要经济地生产大量的用作合成有用物质如药物的中间体的光学活性γ-内酯衍生物（例如泛酸内酯）。为了达到这个目的，使用水解酶内酯酶的不对称水解酶技术是有利的。然而，制备酶或利用能够产生酶的微生物仍然是麻烦的。此外，在使用重组技术的情况下，难以获得足够稳定的酶活性。在制备内含子选择性地水解γ-内酯衍生物如外消旋泛酸内酯的内切酶中，通过重组技术，将成熟内含酶DNA和信号肽区DNA两者转移到宿主中。因此，可以获得与天然存在的活性相当的稳定的内含酶活性，并且可以获得以稳定的方式维持高酶活性的转化体，从而允许有效和工业上有利的γ-内酯衍生物的不对称合成。

9. 发明授权

US09140329B2 Transmission belt 有权
标题翻译：传动皮带
公开(公告)号：US09140329B2
公开(公告)日：2015-09-22
申请号：US14359565
申请日：2012-02-01
申请人： Mitsuhiro Yamada , Keiji Sakamoto , Shinji Uchigashima
发明人： Mitsuhiro Yamada , Keiji Sakamoto , Shinji Uchigashima
IPC分类号： C08F8/00 , C08F283/00 , C08F12/28 , F16G1/10 , F16G1/12 , F16G1/28
CPC分类号： F16G1/10 , F16G1/12 , F16G1/28
摘要： The durability of a belt is enhanced by improving the strength of rubber itself while enhancing its peeling strength with respect to a cord, a fabric or a short fiber. A toothed belt (10) comprises a belt body (13) that is integrally formed by a tooth rubber (11) provided on one surface side thereof, a backing rubber (12) provided on the other surface side, and an embedded cord (14) spirally wound in a boundary portion between the tooth rubber (11) and the backing rubber (12) that extends in the longitudinal direction of the belt. A facing fabric (20) covering the tooth rubber (11) is adhered to a surface of the tooth rubber (11), or one surface of the belt body (13). The tooth rubber (11) is obtained by vulcanization-molding a rubber composition including rubber such as HNBR, resorcinol, a melamine compound, silica and the like.
摘要翻译：通过提高橡胶本身的强度并提高其对帘线，织物或短纤维的剥离强度，可以提高带的耐久性。齿形带（10）包括带状体（13），其由设置在其一个表面侧上的齿状橡胶（11），设置在另一个表面侧的背衬橡胶（12）和嵌入的帘线）螺旋缠绕在沿着带的纵向方向延伸的齿橡胶（11）和背衬橡胶（12）之间的边界部分中。覆盖齿橡胶（11）的面料（20）粘附到齿橡胶（11）的表面或带体（13）的一个表面。通过硫化成型包含橡胶如HNBR，间苯二酚，三聚氰胺化合物，二氧化硅等的橡胶组合物来获得齿橡胶（11）。

10. 发明授权

US08587716B2 Lens barrel, image pickup device, and lens barrel manufacturing method 有权
公开(公告)号：US08587716B2
公开(公告)日：2013-11-19
申请号：US13270584
申请日：2011-10-11
申请人： Eiichi Nagaoka , Yuji Adachi , Keiji Sakamoto
发明人： Eiichi Nagaoka , Yuji Adachi , Keiji Sakamoto
IPC分类号： H04N5/225
CPC分类号： G02B7/021 , G02B5/04 , G02B7/025 , G02B7/10 , G02B7/1805 , G02B15/173 , H04N5/2254 , H04N2101/00 , Y10T29/49885
摘要： A lens barrel includes a fourth lens, a prism, and a sixth lens. The fourth lens receives a light flux incident along a first optical axis. The prism includes a reflecting surface reflecting the light flux passing through the fourth lens to a direction along a second optical axis intersecting with the first optical axis. The sixth lens receives the light flux reflected by the prism. A second group frame includes an opening portion, a prism retaining frame that is arranged in a more inner position than the opening portion and in which the prism is contained, and a plurality of adhesive pockets arranged on an area around the prism retaining frame and being open to the side of the opening portion. Adhesive agent is filled in the adhesive pockets.

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