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1. 发明授权

US4683195A Process for amplifying, detecting, and/or-cloning nucleic acid sequences 失效
标题翻译：用于扩增，检测和/或克隆核酸序列的方法
公开(公告)号：US4683195A
公开(公告)日：1987-07-28
申请号：US828144
申请日：1986-02-07
申请人： Kary B. Mullis , Henry A. Erlich , Norman Arnheim , Glenn T. Horn , Randall K. Saiki , Stephen J. Scharf
发明人： Kary B. Mullis , Henry A. Erlich , Norman Arnheim , Glenn T. Horn , Randall K. Saiki , Stephen J. Scharf
IPC分类号： C12Q1/68 , C12P19/34 , C12N1/00 , C12N15/00 , G01N33/48 , G01N33/00 , G01N33/566 , G01N33/564 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6881 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q2600/156 , Y10T436/143333
摘要： The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.
摘要翻译：本发明涉及扩增和检测包含在核酸或其混合物中的任何靶核酸序列的方法。该方法包括用摩尔过量的两种寡核苷酸引物处理分离的核酸互补链，延伸引物以形成用作合成所需核酸序列的模板的互补引物延伸产物，以及检测如此扩增的序列。反应的步骤可以逐步或同时进行，并且可以根据需要经常重复。此外，通过使用引物扩增其非互补末端含有限制位点的序列，可以将特异性核酸序列克隆到载体中，并且可以使用扩增过程从现有的较短片段制备核酸片段。

2. 发明授权

US4800159A Process for amplifying, detecting, and/or cloning nucleic acid sequences 失效
标题翻译：用于扩增，检测和/或克隆核酸序列的方法
公开(公告)号：US4800159A
公开(公告)日：1989-01-24
申请号：US943948
申请日：1986-12-17
申请人： Kary B. Mullis , Henry A. Erlich , Norman Arnheim , Glenn T. Horn , Randall K. Saiki , Stephen J. Scharf
发明人： Kary B. Mullis , Henry A. Erlich , Norman Arnheim , Glenn T. Horn , Randall K. Saiki , Stephen J. Scharf
IPC分类号： C12Q1/68 , C12N15/00 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6858
摘要： The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.
摘要翻译：本发明涉及扩增和检测包含在核酸或其混合物中的任何靶核酸序列的方法。该方法包括用摩尔过量的两种寡核苷酸引物处理分离的核酸互补链，延伸引物以形成用作合成所需核酸序列的模板的互补引物延伸产物，以及检测如此扩增的序列。反应的步骤可以逐步或同时进行，并且可以根据需要经常重复。此外，通过使用引物扩增其非互补末端含有限制性位点的序列，可以将特异性核酸序列克隆到载体中，并且可以使用扩增过程从现有的较短片段制备核酸片段。

3. 发明授权

US5541065A Method for HLA DP typing 失效
标题翻译： HLA DP分型方法
公开(公告)号：US5541065A
公开(公告)日：1996-07-30
申请号：US195615
申请日：1994-02-14
申请人： Henry A. Erlich , Glenn T. Horn , Teodorica Bugawan , Ann B. Begovich
发明人： Henry A. Erlich , Glenn T. Horn , Teodorica Bugawan , Ann B. Begovich
IPC分类号： C12N15/09 , A61K39/00 , A61K49/00 , C07K14/74 , C12Q1/04 , C12Q1/68 , G01N33/53 , G01N33/566 , C07H21/04
CPC分类号： C12Q1/6881 , C07K14/70539 , C12Q1/6811 , C12Q2600/156
摘要： A process for determining the genotype of an individual with respect to the alleles at the HLA DP locus involves obtaining a sample of nucleic acid from the individual, and hybridizing the nucleic acids with a panel of probes specific for variant segments of DPalpha and DPbeta genes. Because the variation between DPbeta alleles is highly dispersed throughout the second exon of the DPbeta gene, the discovery of many different DPbeta alleles makes the process far more discriminating and informative than cellular, RFLP, or serological methods. The process can also be carried out on amplified nucleic acid produced by the polymerase chain reaction using primers specific for the second exon of the DPalpha and DPbeta genes. HLA DP DNA typing methods are useful in the prevention of graft rejection and host versus graft disease, in determining susceptibility to autoimmune diseases, in providing evidence concerning the derivation from an individual of forensic samples, and in paternity testing.
摘要翻译：用于确定个体相对于HLA DP位点处的等位基因的基因型的方法包括从个体获得核酸样品，并将核酸与DPalpha和DPbeta基因的变体片段特异性的一组探针杂交。因为DPbeta等位基因之间的差异在DPbeta基因的第二个外显子中高度分散，许多不同的DPbeta等位基因的发现使得该过程比细胞，RFLP或血清学方法更具歧视性和信息性。该方法还可以通过聚合酶链式反应产生的扩增核酸进行，其使用对DPalpha和DPbeta基因的第二外显子特异的引物。 HLA DP DNA分型方法可用于预防移植排斥反应和宿主与移植物疾病，确定对自身免疫性疾病的易感性，提供有关法医样本个体的推导和亲子鉴定的证据。

4. 发明授权

US5665548A Characterization and detection of sequences associated with autoimmune diseases 失效
标题翻译：表征和检测与自身免疫疾病相关的序列
公开(公告)号：US5665548A
公开(公告)日：1997-09-09
申请号：US448021
申请日：1995-05-23
申请人： Henry A. Erlich , Glenn T. Horn
发明人： Henry A. Erlich , Glenn T. Horn
IPC分类号： G01N33/53 , A61K38/00 , A61K39/395 , A61P37/00 , C07K14/74 , C07K16/28 , C12N15/02 , C12N15/09 , C12Q1/68 , G01N33/564 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6883 , C07K14/70539 , C07K16/2833 , C12Q1/686 , C12Q1/6881 , A61K38/00 , C12Q2600/156 , C12Q2600/172
摘要： DNA sequences and corresponding amino acid sequences from the HLA class II beta region of the human genome that are associated with insulin-dependent diabetes mellitus (IDDM) and Pemphigus vulgaris (PV) have been identified. Specifically, marker DNA sequences which detect either directly or indirectly the identity of the codon encoding for the amino acid at position 57 of the DQ.beta. protein sequence are disclosed as well as sequences from the DR.beta. region. These sequences may be used to generate DNA hybridization probes and antibodies for assays to detect a person's susceptibility to autoimmune diseases, such as IDDM and PV. Such antibodies and peptides encoded by said DNA sequences can be used therapeutically or prophylactically.
摘要翻译：已经鉴定了与胰岛素依赖性糖尿病（IDDM）和寻常型天疱疮（PV）相关的人类基因组的HLA II类β区的DNA序列和相应的氨基酸序列。具体地，公开了直接或间接检测编码DQβ蛋白序列的位置57的氨基酸的密码子的身份的标记DNA序列，以及来自DRβ区的序列。这些序列可用于产生DNA杂交探针和用于检测个体对自身免疫性疾病如IDDM和PV的易感性的测定的抗体。由所述DNA序列编码的这样的抗体和肽可以用于治疗或预防。

5. 发明授权

US06194561B1 Characterization and detection of sequences associated with autoimmune diseases 失效
标题翻译：表征和检测与自身免疫疾病相关的序列
公开(公告)号：US06194561B1
公开(公告)日：2001-02-27
申请号：US07121519
申请日：1987-11-17
申请人： Henry A. Erlich , Glenn T. Horn
发明人： Henry A. Erlich , Glenn T. Horn
IPC分类号： C07H2104
CPC分类号： C12Q1/6883 , A61K38/00 , C07K14/70539 , C07K16/2833 , C12Q1/686 , C12Q1/6881 , C12Q2600/156 , C12Q2600/172
摘要： DNA sequences and corresponding amino acid sequences from the HLA class II beta region of the human genome that are associated with insulin-dependent diabetes mellitus (IDDM) and Pemphigus vulgaris (PV) have been identified. Specifically, marker DNA sequences which detect either directly or indirectly the identity of the codon encoding for the amino acid at position 57 of the DQ&bgr; protein sequence are disclosed as well as sequences from the DR&bgr; region. These sequences may be used to generate DNA hybridization probes and antibodies for assays to detect a person's susceptibility to autoimmune diseases, such as IDDM and PV. Such antibodies and peptides encoded by said DNA sequences can be used therapeutically or prophylactically.
摘要翻译：已经鉴定了与胰岛素依赖性糖尿病（IDDM）和寻常型天疱疮（PV）相关的人类基因组的HLA II类β区的DNA序列和相应的氨基酸序列。具体地，公开了直接或间接检测编码DQbeta蛋白序列第57位的氨基酸的密码子的同一性的标记DNA序列以及来自DRbeta区的序列。这些序列可用于产生DNA杂交探针和用于检测个体对自身免疫性疾病如IDDM和PV的易感性的测定的抗体。由所述DNA序列编码的这样的抗体和肽可以用于治疗或预防。

6. 发明授权

US5310893A Method for HLA DP typing 失效
标题翻译： HLA DP分型方法
公开(公告)号：US5310893A
公开(公告)日：1994-05-10
申请号：US347506
申请日：1989-05-04
申请人： Henry A. Erlich , Glenn T. Horn , Teodorica Bugawan , Ann B. Begovich
发明人： Henry A. Erlich , Glenn T. Horn , Teodorica Bugawan , Ann B. Begovich
IPC分类号： C12N15/09 , A61K39/00 , A61K49/00 , C07K14/74 , C12Q1/04 , C12Q1/68 , G01N33/53 , G01N33/566 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/6881 , C07K14/70539 , C12Q1/6811 , C12Q2600/156
摘要： A process for determining the genotype of an individual with respect to the alleles at the HLA DP locus involves obtaining a sample of nucleic acid from the individual, and hybridizing the nucleic acids with a panel of probes specific for variant segments of DPalpha and DPbeta genes. Because the variation between DPbeta alleles is highly dispersed throughout the second exon of the DPbeta gene, the discovery of many different DPbeta alleles makes the process far more discriminating and informative than cellular, RFLP, or serological methods. The process can also be carried out on amplified nucleic acid produced by the polymerase chain reaction using primers specific for the second exon of the DPalpha and DPbeta genes. HLA DP DNA typing methods are useful in the prevention of graft rejection and host versus graft disease, in determining susceptibility to autoimmune diseases, in providing evidence concerning the derivation from an individual of forensic samples, and in paternity testing.
摘要翻译：用于确定个体相对于HLA DP位点处的等位基因的基因型的方法包括从个体获得核酸样品，并将核酸与DPalpha和DPbeta基因的变体片段特异性的一组探针杂交。因为DPbeta等位基因之间的差异在DPbeta基因的第二个外显子中高度分散，许多不同的DPbeta等位基因的发现使得该过程比细胞，RFLP或血清学方法更具歧视性和信息性。该方法还可以通过聚合酶链式反应产生的扩增核酸进行，其使用对DPalpha和DPbeta基因的第二外显子特异的引物。 HLA DP DNA分型方法可用于预防移植排斥反应和宿主与移植物疾病，确定对自身免疫性疾病的易感性，提供有关法医样本个体的推导和亲子鉴定的证据。

7. 发明授权

US5538868A Recombinant ricin toxin fragments 失效
标题翻译：重组蓖麻毒素片段
公开(公告)号：US5538868A
公开(公告)日：1996-07-23
申请号：US376286
申请日：1995-01-23
申请人： Glenn T. Horn , Michael Piatak, Jr.
发明人： Glenn T. Horn , Michael Piatak, Jr.
IPC分类号： C07K14/415 , C12N15/10 , C12N15/69 , C12N15/70 , C12P19/34 , C12N15/09 , C12N15/63
CPC分类号： C12N15/69 , C07K14/415 , C12N15/10 , C12N15/70
摘要： Recombinant vectors and methods for constructing ricin B are disclosed. The coding sequence for ricin B was cloned, disposed in suitable expression vectors and produced free of components normally accompanying this peptide. In addition, a novel means of reconstructing missing portions of coding sequence, and certain improvements in messenger RNA purification are disclosed.
摘要翻译：公开了重组载体和构建蓖麻毒蛋白B的方法。将蓖麻蛋白B的编码序列克隆，置于合适的表达载体中，并且不含通常伴随该肽的成分产生。另外，公开了一种重建编码序列缺失部分的新方法，并揭示了信使RNA纯化中的某些改进。

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