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1. 发明公开

EP3106513A1 METHOD FOR DECOMPOSING POLYHYDROXYALKANOIC ACID, AND MICROORGANISM PREPARATION 审中-公开
标题翻译： VERFAHREN ZUR ZERSETZUNG VONPOLYHYDROXYALKANSÄURE，HERSTELLUNG UND MIKROORGANISMUSZUBEREITUNG
公开(公告)号：EP3106513A1
公开(公告)日：2016-12-21
申请号：EP15749199.4
申请日：2015-02-12
申请人： Kaneka Corporation , Niigata University Of Pharmacy And Applied Life Sciences
发明人： SHIGEMATSU, Toru , IGUCHI, Akinori , SUGAYA, Takahiko , MATSUMOTO, Keiji
IPC分类号： C12N1/00 , C02F3/34 , C08J11/10 , C12N1/20
CPC分类号： C02F3/34 , C12N1/20 , C12R1/01
摘要： Provided are methods for decomposing a polyhydroxyalkanoic acid using an anaerobic microbial community for decomposing a polyhydroxyalkanoic acid efficiently to convert the polyhydroxyalkanoic acid to a biogas including methane as a main component; and a microbial preparation usable as seed sludge for a methane fermentation process. The polyhydroxyalkanoic acid is subjected to decomposing treatment with a microbial community including a methane-producing archaeon in the following (A), and a bacterium in the following (B): (A) a methane-producing archaeon belonging to the genus Methanosarcina, or a single-rod-shaped hydrogen-utilizing methane-producing archaeon; and (B) a bacterium which is classified into any one of the phylum Acidobacteria, the phylum Bacteroidetes, the phylum Chloroflexi, the phylum Firmicutes, the phylum Nitrospirae, the phylum Proteobacteria, the phylum Synergistetes, the phylum Thermotogae and an unclassified phylum, and is capable of decomposing the polyhydroxyalkanoic acid.
摘要翻译：提供了使用厌氧微生物群落分解聚羟基链烷酸的方法，用于有效地分解聚羟基链烷酸以将聚羟基链烷酸转化为包括甲烷作为主要成分的生物气体; 以及可用作甲烷发酵过程的种子污泥的微生物制剂。将聚羟基链烷酸在包含以下（A）中的产生甲烷的考古的微生物群落和下列（B）的细菌中进行分解处理：（A）甲烷属的甲烷生产古细菌，或单杆式氢利用甲烷生产考古; 和（B）细菌，其分为任何一种门酸杆菌，致病杆菌门，Chloroflexi门，灵芝菌，硝腺菌门，变形菌门，门协同疗法，门藻类和未分类的门，以及能够分解聚羟基链烷酸。

2. 发明公开

EP3492581A1 TRANSFORMANT THAT PRODUCES PHA COPOLYMER CONTAINING 3HH UNIT, AND METHOD FOR PRODUCING SAID PHA 审中-公开
公开(公告)号：EP3492581A1
公开(公告)日：2019-06-05
申请号：EP17834054.3
申请日：2017-07-13
申请人： Kaneka Corporation
发明人： ARIKAWA, Hisashi , MATSUMOTO, Keiji
IPC分类号： C12N1/21 , C12P7/62 , C12N15/09
摘要： Provided are a transformant that produces a copolymerized PHA containing 3HH units in a higher composition proportion; and a method for producing a copolymerized PHA, using this transfonnant. The transformant is a transformant that produces a copolymerized PHA containing 3HH units, in which a gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is introduced into a prokaryotic microorganism having a PHA synthetase gene capable of synthesizing the copolymerized PHA containing the 3HH units. The method is a method for producing a copolymerized PHA containing 3HH units, which includes a step of culturing this transformant.

3. 发明公开

EP3101129A1 MICROORGANISM HAVING ADJUSTED EXPRESSION OF R-SPECIFIC ENOYL-COA HYDRATASE GENE, AND METHOD FOR MANUFACTURING POLYHYDROXYALKANOATE COPOLYMER USING SAME 有权
标题翻译：微生物有适于特定R-烯酰-CoA HYDRATASEGEN表达和工艺SO产生聚羟基烷酸酯共聚物
公开(公告)号：EP3101129A1
公开(公告)日：2016-12-07
申请号：EP15742617.2
申请日：2015-01-30
申请人： Kaneka Corporation
发明人： ARIKAWA, Hisashi , SATO, Shunsuke , MATSUMOTO, Keiji
IPC分类号： C12N15/09 , C12N1/21 , C12P7/62
摘要： This invention relates to a microorganism that produces a polyhydroxyalkanoate (PHA) copolymer with a regulated monomer composition ratio and comprises a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification consisting of a substitution(s), a deletion(s), an insertion(s), and/or an addition(s) of one or a plurality of nucleotides so that the expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and to a method for producing a PHA copolymer using the microorganism.
摘要翻译：本发明涉及的微生物没有产生一个与调节单体组成比的聚羟基烷酸酯（PHA）共聚物，并包括在基因组DNA中的（R） - 特异性烯酰辅酶A水合酶基因worin的核苷酸序列的（R）的上游-Specific 烯酰基-CoA水合酶基因包含修饰由置换（一个或多个），缺失（多个），（插入（多个）和/或在一个另外的（一个或多个）或核苷酸的多元性所以没有的表达 R）-Specific烯酰辅酶A水合酶基因被调节，以及一种用于制造使用该微生物的PHA共聚物的方法。

4. 发明公开

EP1550723A1 METHOD OF PURIFYING 3-HYDROXYALKANOIC ACID COPOLYMER 有权
标题翻译： VERFAHREN ZUR REINIGUNG VON 3-羟基甘露醇共聚物
公开(公告)号：EP1550723A1
公开(公告)日：2005-07-06
申请号：EP03798554.6
申请日：2003-09-30
申请人： KANEKA CORPORATION
发明人： OGAWA, Noriko , MIYAMOTO, K., 101, Nikkanhaimu Dai 8 , OSAKADA, Fumio , MATSUMOTO, Keiji
IPC分类号： C12P7/62 , C08G63/90
CPC分类号： C08G63/90 , C08G63/06
摘要： The object of this invention is to provide a method for purifying PHA separated from PHA-containing microbial cells in high purity without incurring any serious decrease in molecular weight.
The present invention provides a method for purifying a 3-hydroxyalkanoic acid copolymer produced by a microorganism,
which comprises treating an aqueous suspension containing the 3-hydroxyalkanoic acid copolymer separated from a microorganism with a hydrogen peroxide while controlling the pH of said aqueous suspension by adding an alkali either continuously or intermittently.
摘要翻译：本发明的目的是提供一种从高纯度纯化含PHA微生物细胞分离PHA的方法，而不会导致分子量的严重下降。本发明提供了一种纯化由微生物制备的3-羟基链烷酸共聚物的方法，该方法包括用含有过氧化氢的微生物分离的含有3-羟基链烷酸共聚物的含水悬浮液进行处理，同时控制所述水悬浮液，通过连续或间歇地加入碱。

5. 发明公开

EP1352958A1 GENE DISRUPTED YEAST 审中-公开
标题翻译： HEFE MIT UNTERBROCHENEM GEN
公开(公告)号：EP1352958A1
公开(公告)日：2003-10-15
申请号：EP02716318.7
申请日：2002-01-18
申请人： KANEKA CORPORATION
发明人： FUKUCHI, Ken , YOKOMIZO, Satoru , OSAKADA, Fumio , MATSUMOTO, Keiji , TAKAGI, Masamichi , OTA, Akinori
IPC分类号： C12N15/09 , C12N1/19 , C12P7/62
CPC分类号： C12N9/93 , C12P7/625 , C12Y603/02006
摘要： The invention provides a yeast in which a specific gene alone has been disrupted and to which auxotrophy is impared to construct a system for producing an industrially useful substance through gene recombination by utilizing characteristics of a yeast.
In the practice of the invention, a yeast strain in which ADE1 gene has been disrupted is constructed by homologous recombination with a chromosomal DNA by using an ADE1 gene fragment encoding phosphoribosylaminoimidazole succinocarboxamide synthase (EC6.3.2.6) of Candida maltosa. In the practice of the invention, then, this disrupted strain is transformed by a gene expression cassette containing a biodegradable polyester synthase gene and the obtained transformant is cultured to thereby produce biodegradable polyester.
摘要翻译：本发明提供了一种酵母，其中单独的特定基因已经被破坏并且通过利用酵母的特性通过基因重组构建了用于产生工业上有用物质的制剂的营养缺陷型。在本发明的实践中，通过使用编码拟南芥假丝酵母的磷酸核糖氨基咪唑琥珀酰胺合成酶（EC6.3.2.6）的ADE1基因片段，通过与染色体DNA的同源重组构建其中ADE1基因被破坏的酵母菌株。在本发明的实践中，通过含有可生物降解的聚酯合酶基因的基因表达盒转化该破坏的菌株，培养得到的转化体，从而生成可生物降解的聚酯。

6. 发明公开

EP0987332A1 PROCESS FOR PRODUCING OPTICALLY ACTIVE AMINO COMPOUNDS 失效
标题翻译： Deoxynukleinsäure编码具有立体选择性转氨酶活性，和含有该Deoxynukleinsäure转化的多肽
公开(公告)号：EP0987332A1
公开(公告)日：2000-03-22
申请号：EP98914111.4
申请日：1998-04-20
申请人： Kaneka Corporation
发明人： YAMADA, Yukio , IWASAKI, Akira , KIZAKI, Noriyuki , MATSUMOTO, Keiji , IKENAKA, Yasuhiro , OGURA, Masahiro , HASEGAWA, Junzo
IPC分类号： C12P13/04 , C12N15/54
CPC分类号： C12P13/001 , C12N9/1096
摘要： A process for efficiently producing optically active amino compounds, mainly (R)-amino compounds, at a low cost by means of a microbial enzyme; a polypeptide suitable for use in the production process and having a stereoselective transaminase activity; and a DNA coding for the polypeptide. The process is characterized by comprising causing a transaminase to act on a ketone compound as an amino acceptor in the presence of a primary amine as an amino donor to stereoselectively transfer the amino group to thus obtain an optically active amino compound. The DNA contains a base sequence coding for a polypeptide having a stereoselective transaminase activity. The polypeptide can be obtained by incubating a microorganism belonging to the genus Arthrobacter .
摘要翻译：一种用于有效地制造光学活性氨基化合物，主要（R）的氨基化合物，在通过微生物酶的手段低成本过程; 适合于在生产过程中使用，并且具有立体选择性转氨酶活性的多肽; 和DNA编码的多肽。该过程由包括使转氨酶在伯胺的存在下在酮化合物作为氨基受体的作为氨基供体的立体选择性地转移的氨基，得到因而光学活性氨基化合物为特征的。的DNA含有的碱基序列编码具有立体选择性转氨酶活性的多肽。所述多肽可通过温育属于节杆菌属的微生物来获得。

7. 发明公开

EP2910641A1 HIGH MOLECULAR WEIGHT PHA-PRODUCING MICROBE AND METHOD OF PRODUCING HIGH MOLECULAR WEIGHT PHA USING SAME 有权
标题翻译：生殖FOR PHA高分子量和方法的高分子量与PHA的生产制作
公开(公告)号：EP2910641A1
公开(公告)日：2015-08-26
申请号：EP13848540.4
申请日：2013-10-22
申请人： Kaneka Corporation
发明人： ARIKAWA, Hisashi , SATO, Shunsuke , MATSUMOTO, Keiji
IPC分类号： C12P7/62 , C12N15/09
CPC分类号： C12P7/625 , C12N9/18 , C12N15/74
摘要： An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.
摘要翻译：本发明的一个目的是提供一种微生物菌株做累加的高分子量PHA和使用该微生物的PHA生产方法。本发明提供的方法用于制造PHA共聚物，其包括培养微生物，worin至少下列基因（a）和任的部分（b）的微生物已经被改变通过取代，缺失，插入，和 /或添加，以减少或消除由该基因编码的PHA降解酶的活性：（a）编码的SEQ ID NO的氨基酸序列的PHA降解酶基因：序列表中2; 和（b）编码的基因具有至少85％序列同一性的SEQ ID NO的氨基酸序列的多肽：所述序列中列出2和具有PHA降解酶活性。

8. 发明公开

EP1726637A1 NOVEL TRANSFORMANT AND PROCESS FOR PRODUCING POLYESTER BY USING THE SAME 审中-公开
标题翻译： VERFAHRENZUR HERSTELLUNG VON POLYESTER UNTER VERWENDUNG DAVON
公开(公告)号：EP1726637A1
公开(公告)日：2006-11-29
申请号：EP05719893.9
申请日：2005-03-03
申请人： Kaneka Corporation
发明人： OKUBO, Yuji , MATSUMOTO, Keiji , TAKAGI, Masamichi , OHTA, Akinori
IPC分类号： C12N1/19 , C12N15/09 , C12P7/62
CPC分类号： C12N9/1025 , C12N9/0006 , C12N9/1096 , C12N9/88 , C12N9/93 , C12P7/625
摘要： The present invention provides a process for producing yeast excellent in cell productivity and gene manipulation of which is easy, being added with nutritional requirement by disrupting only a specific gene, and a transformant thereof. Moreover, the present invention also provides a process for producing a gene expression product, particularly a polyhydroxyalkanoic acid.
In the present invention, yeast in which a plurality of genes is disrupted is produced using the homologous recombination. Moreover, a transformant is obtained by introducing a plurality of enzyme genes involved with polyhydroxyalkanoic acid synthesis such as a polyhydroxyalkanoic acid synthase gene and an acetoacetyl CoA reductase gene into said gene-disrupted yeast. Furthermore, said transformant is cultured, copolyesters comprising a polyhydroxyalkanoic acid are efficiently accumulated within the cells, and a polymer is harvested from the cultured product.
摘要翻译：本发明提供一种生产酵母的方法，其中容易的细胞生产力和基因操作优良，通过仅破坏特定基因而加入营养需求及其转化体。此外，本发明还提供了生产基因表达产物，特别是聚羟基链烷酸的方法。在本发明中，使用同源重组产生多个基因被破坏的酵母。此外，通过将涉及聚羟基链烷酸合成的多种酶基因如聚羟基链烷酸合酶基因和乙酰乙酰辅酶A还原酶基因引入所述基因破坏的酵母中来获得转化体。此外，培养所述转化体，包含聚羟基链烷酸的共聚酯有效地积聚在细胞内，并从培养产物中收获聚合物。

9. 发明公开

EP1626087A1 IMPROVED TRANSFORMANT AND PROCESS FOR PRODUCING POLYESTER USING THE SAME 审中-公开
标题翻译： VERBESSERTER变压器VERFAHREN ZUR HERSTELLUNG VON POLYESTER DAMIT
公开(公告)号：EP1626087A1
公开(公告)日：2006-02-15
申请号：EP04733092.3
申请日：2004-05-14
申请人： KANEKA CORPORATION
发明人： OKUBO, Yuji , NAGAOKA, Tetsuya , YOKOMIZO, Satoru , MATSUMOTO, Keiji , TAKAGI, Masamichi , OHTA, Akinori
IPC分类号： C12N15/52 , C12N1/19 , C12P7/62 , C12R1/72
CPC分类号： C12N9/1025 , C12P7/625
摘要： This invention relates to provide a gene expression cassette, which comprises a gene coding for an Aeromonas caviae-derived polyhydroxyalkanoic acid (PHA) synthase wherein a mutation has been introduced, and a promoter and a terminator both capable of functioning in yeast; a transformant wherein said gene expression cassette has been introduced in yeast, and a method for producing polyester by using said transformant.
This invention enabled a production of copolyesters resulting from copolymerization of a 3-hydroxyalkanoic acid(s) and having biodegradability and good physical properties in yeast efficiently.
摘要翻译：本发明涉及提供一种基因表达盒，其包含编码引入突变的气单胞菌来源的聚羟基链烷酸（PHA）合酶的基因，以及能够在酵母中起作用的启动子和终止子; 其中所述基因表达盒已经引入酵母中的转化体，以及通过使用所述转化体生产聚酯的方法。本发明能够生产由3-羟基烷酸共聚得到的共聚酯，并且在酵母中具有良好的生物降解性和良好的物理性能。

10. 发明公开

EP1441031A1 ENZYME GENE PARTICIPATING IN THE SYNTHESIS OF POLYESTER AND PROCESS FOR PRODUCING POLYESTER USING THE SAME 审中-公开
标题翻译：酶基因，AT聚酯的合成有关，和方法;聚酯使用该基因
公开(公告)号：EP1441031A1
公开(公告)日：2004-07-28
申请号：EP02801508.9
申请日：2002-10-09
申请人： KANEKA CORPORATION
发明人： YOKOMIZO, Satoru , FUKUCHI, Takeshi , OSAKADA, Fumio , MATSUMOTO, Keiji , TAKAGI, Masamichi , OHTA, Akinori
IPC分类号： C12N15/52 , C12N15/81 , C12N1/19 , C12P7/62
CPC分类号： C07K14/40 , C07K2319/01 , C12N9/88 , C12N15/52 , C12P7/625
摘要： This invention relates to a method of producing polyesters resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) and having biodegradability and good physical properties using yeasts as hosts.
By constructing at least one enzyme gene involved in polyester synthesis by adding a DNA coding a peroxisome-targeting signal, introducing a enzyme gene expression cassette containing that gene into yeast, and cultivating the thus-obtained transformant, it becomes possible to cause accumulation of a polyester resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) in yeast cells and recover the polyester from the culture.
摘要翻译：本发明涉及生产聚酯从均聚或3-羟基链烷酸的共聚得到的（S）和具有生物降解性，并使用酵母作为宿主良好的物理性能的方法。通过添加一个DNA编码过氧化物酶体靶向信号，引入酶基因表达盒做市含基因导入酵母，培养由此获得的转化体构建在聚酯合成有关的至少一种酶的基因，也能够聚酯从在酵母细胞中的3-羟基烷酸（S）的均聚或共聚得到的的原因的积累和恢复从培养物中的聚酯。

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