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1. 发明公开

EP2147973A1 GENE-DISRUPTED STRAIN, RECOMBINANT PLASMID, TRANSFORMANT AND METHOD OF PRODUCING 3-CARBOXYMUCONOLACTONE 审中-公开
标题翻译：具有精确的电路，重组质粒转化及其制造方法3 CARBOXYMUCONOLACTON STEM
公开(公告)号：EP2147973A1
公开(公告)日：2010-01-27
申请号：EP08752842.8
申请日：2008-05-09
申请人： KABUSHIKI KAISHA TOYOTA JIDOSHOKKI , National University Corporation Tokyo University of Agriculture and Technology , Nagaoka University of Technology , FORESTRY AND FOREST PRODUCTS RESEARCH INSTITUTE
发明人： SHIMO, Toshihisa , MASE, Kohei , KATAYAMA, Yoshihiro , MASAI, Eiji , FUKUDA, Masao , SHIGEHARA, Kiyotaka , OHARA, Seiji , NAKAMURA, Masaya , OTSUKA, Yuichiro
IPC分类号： C12N15/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12P7/44 , C12P17/04
CPC分类号： C12P7/48 , C12N9/0069 , C12P17/04 , C12Y113/11003
摘要： Industrial-scale fermentative production of 3-carboxy-cis,cis-muconic acid from terephthalic acid.
Also, a protocatechuate 4,5-ring-cleaving enzyme gene-disrupted strain in which the gene coding for (a) the amino acid sequence set forth in SEQ ID NO: 1 or 3, or (b) the amino acid sequence set forth in SEQ ID NO: 1 or 3 which has a deletion, substitution, addition and/or insertion of one or more amino acids and exhibits protocatechuate 4,5-ring cleavage activity, present in the chromosomal DNA of microbial cells, has been disrupted; recombinant plasmids comprising the Tph gene and protocatechuate 3,4-dioxygenase gene; transformants obtained by introducing the recombinant plasmids into the disrupted strain; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of terephthalic acid.
摘要翻译：工业规模发酵生产3-羧基 - 顺，顺 - 粘康来自对苯二甲酸的酸。因此，一个原儿茶酸酯4,5-环 - 切割酶基因被破坏的菌株，其中阐述了（a）所述的氨基酸序列的基因编码在SEQ ID NO：1或3，或（b）的氨基酸序列阐述在SEQ ID NO：1或3，其具有缺失，取代，添加和/或一个或多个氨基酸和展品插入儿茶酸酯4,5-环切割活性，存在于微生物细胞的染色体DNA中，已被破坏; 重组质粒包含了phr基因和原儿茶酸3,4-双加氧酶基因; 通过将重组质粒导入的被破坏的菌株获得的转化体; 和用于生产3-羧基 - 顺，顺 - 黏康酸和/或3-羧基通过在对苯二甲酸存在下培养转化体为特征的方法。

2. 发明公开

EP2048231A1 PLASMID, TRANSFORMANT, AND METHOD FOR PRODUCTION OF 3-CARBOXYMUCONOLACTONE 失效转让
标题翻译： PLASMID，TRANSFORMANTE UND VERFAHREN ZUR HERSTELLUNG VON 3-CARBOXYMUCONOLACTON
公开(公告)号：EP2048231A1
公开(公告)日：2009-04-15
申请号：EP07792616.0
申请日：2007-08-10
申请人： KABUSHIKI KAISHA TOYOTA JIDOSHOKKI , National University Corporation Tokyo University of Agriculture and Technology , Nagaoka University of Technology , FORESTRY AND FOREST PRODUCTS RESEARCH INSTITUTE
发明人： MASE, Kohei , SHIMO, Toshihisa , OHARA, Naoki , KATAYAMA, Yoshihiro , SHIGEHARA, Kiyotaka , MASAI, Eiji , FUKUDA, Masao , OHARA, Seiji , NAKAMURA, Masaya , OTSUKA, Yuichiro
IPC分类号： C12N15/00 , C12N1/21 , C12N15/09 , C12P7/26 , C12P7/40
CPC分类号： C12N9/1007 , C12N9/0008 , C12N9/0069 , C12P17/04
摘要： There is provided a process for industrial production of simple 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone from low molecular mixtures derived from plant components such as vanillin, vanillic acid and protocatechuic acid, via a multistage enzyme reaction.
A recombinant plasmid containing a vanillate demethylase gene (vanAB genes), benzaldehyde dehydrogenase gene (ligV gene) and protocatechuate 3,4-dioxygenase gene (pcaHG genes); transformants incorporating the plasmid; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of vanillin, vanillic acid, protocatechuic acid or a mixture of two or more thereof.
摘要翻译：提供了一种通过多级酶反应从由植物成分如香草醛，香草酸和原儿茶酸衍生的低分子混合物工业生产简单的3-羧基 - 顺式，顺式 - 粘酸和/或3-羧基粘土内酯的方法。含有香草脱甲基酶基因（vanab基因），苯甲醛脱氢酶基因（ligV基因）和原儿茶素3,4-双加氧酶基因（pcaHG基因）的重组质粒; 掺入质粒的转化体; 以及生产3-羧基 - 顺式，顺式 - 粘酸和/或3-羧基粘土内酯的方法，其特征在于在香草醛，香草酸，原儿茶酸或其两种或更多种的混合物存在下培养转化体。

3. 发明公开

EP2325227A1 POLYESTER, POLYURETHANE AND METHODS FOR PRODUCING SAME 审中-公开
标题翻译： POLYESTER，POLYURETHAN UND HERSTELLUNGSVERFAHRENDAFÜR
公开(公告)号：EP2325227A1
公开(公告)日：2011-05-25
申请号：EP09813171.7
申请日：2009-09-09
申请人： Kabushiki Kaisha Toyota Jidoshokki , National University Corporation Tokyo University of Agriculture and Technology , Nagaoka University of Technology , Forestry And Forest Products Research Institute
发明人： TAKASHIMA, Hiroaki , YAMAMOTO, Yusuke , MASE, Kohei , SHIMO, Toshihisa , ABE, Tomokuni , SHIGEHARA, Kiyotaka , KATAYAMA, Yoshihiro , MASAI, Eiji , NAKAMURA, Masaya , OTSUKA, Yuichiro , OHARA, Seiji
IPC分类号： C08G63/16 , C07D307/58 , C08G18/42 , C08L101/16
CPC分类号： C07D307/58 , C08G18/4269 , C08G18/73 , C08G63/553
摘要： The invention provides biodegradable polyesters, polyurethanes, comprising 3-carboxymuconolactone as a structural unit, 3-carboxymuconolactone esters and a process for their production.
Polyesters comprising a repeating unit represented by the following formula (I):

[wherein R represents a hydrocarbon-based divalent residue which, in the structure, optionally contains a heteroatom without an active hydrogen], polyurethanes comprising the polyester structure; 3-carboxymuconolactone esters; and a process for their production.
摘要翻译：本发明提供了可生物降解的聚酯，包含3-羧基粘土内酯作为结构单元的聚氨酯，3-羧基果糖内酯酯及其生产方法。包含由下式（I）表示的重复单元的聚酯：[其中R表示在结构中任选地含有不含活性氢的杂原子的烃基二价残基]，包含聚酯结构的聚氨酯; 3-羧基果胶内酯酯; 和一个生产过程。

4. 发明授权

EP2048231B1 PLASMID, TRANSFORMANT, AND METHOD FOR PRODUCTION OF 3-CARBOXYMUCONOLACTONE 失效转让
标题翻译：质粒变换ANTE及其制造方法3 CARBOXYMUCONOLACTON
公开(公告)号：EP2048231B1
公开(公告)日：2013-11-06
申请号：EP07792616.0
申请日：2007-08-10
申请人： Forestry And Forest Products Research Institute
发明人： MASE, Kohei , SHIMO, Toshihisa , OHARA, Naoki , KATAYAMA, Yoshihiro , SHIGEHARA, Kiyotaka , MASAI, Eiji , FUKUDA, Masao , OHARA, Seiji , NAKAMURA, Masaya , OTSUKA, Yuichiro
IPC分类号： C12N15/00 , C12N1/21 , C12N15/09 , C12P7/26 , C12P7/40
CPC分类号： C12N9/1007 , C12N9/0008 , C12N9/0069 , C12P17/04

5. 发明公开

EP4119669A1 METHOD FOR PRODUCING 2-PYRONE-4,6-DICARBOXYLIC ACID 审中-实审
公开(公告)号：EP4119669A1
公开(公告)日：2023-01-18
申请号：EP21768052.9
申请日：2021-03-12
申请人： Forest Research and Management Organization , Kantechs Co. Ltd , National University Corporation Nagaoka University of Technology
发明人： NAKAMURA, Masaya , OTSUKA, Yuichiro , KAMEYAMA, Toshiji , KAMEYAMA, Koki , MASAI, Eiji , KAMIMURA, Naofumi , KATAYAMA, Yoshihiro
IPC分类号： C12P7/44 , C12N1/21
摘要： Provided is a method for producing 2-pyrone-4,6-dicarboxylic acid (PDC) by culturing a microorganism that produces PDC. The present invention provides a method of producing PDC by culturing a microorganism that produces 2-pyrone-4,6-dicarboxylic acid (PDC), wherein the method comprises: dissolving the starting substance for production of PDC in a buffer solution that contains no alkali metals, and adjusting the pH of a culture solution with a buffer solution that contains no alkali metals.

6. 发明公开

EP2769821A1 INJECTION APPARATUS 审中-公开
标题翻译：注射装置
公开(公告)号：EP2769821A1
公开(公告)日：2014-08-27
申请号：EP12842293.8
申请日：2012-10-02
申请人： Kabushiki Kaisha Toyota Jidoshokki
发明人： YAMAGUCHI, Kazuyuki , NAKAMURA, Masaya , OMIYA, Kenji
IPC分类号： B29C45/53 , B22D17/32 , B29C45/76
CPC分类号： B29C45/77 , B22D17/10 , B22D17/32 , B29C45/531 , B29C45/76 , B29C2945/76578 , B29C2945/76605 , B29C2945/76658 , B29C2945/76692
摘要： An injection apparatus includes a pressure accumulating portion connected to an operation chamber of an injection cylinder. A control unit controls the speed at which the injection cylinder moves and is connected to a rod of the injection cylinder. The control unit includes a rotary shaft that follows the movement of the rod and is capable of moving in the same direction as the rod, a conversion mechanism that converts the linear motion of the rod into the rotation of the rotary shaft, and a rotation member coupled to the rotary shaft. The control unit further includes a resistance generating member that generates a frictional resistance by coming into sliding contact with the rotation member, an actuating member that is moved by an electric drive source, and a movement mechanism that follows the movement of the actuating member and moves the resistance generating member relative to the rotation member.
摘要翻译：注射装置包括连接到注射缸的操作室的蓄压部分。控制单元控制注射缸移动并连接注射缸的杆的速度。控制单元包括：旋转轴，其跟随杆的移动并且能够沿着与杆相同的方向移动;转换机构，其将杆的线性运动转换为旋转轴的旋转;以及旋转构件连接到旋转轴。控制单元还包括：电阻产生构件，其通过与旋转构件滑动接触而产生摩擦阻力;致动构件，其由电驱动源移动;以及移动机构，其跟随致动构件的移动并移动阻力产生部件相对于旋转部件。

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