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1. 发明申请

WO2009128644A3 GENOME-SCALE BUTANOL-PRODUCING MICROORGANISM METABOLIC NETWORK MODEL AND METHODS USING THE SAME FOR ANALYSIS OF METABOLIC CHARACTERISTICS OF BUTANOL-PRODUCING MICROORGANISMS AND FOR SCREENING DELETION TARGETS 审中-公开
标题翻译：基因组规模的丁醇生产微生物代谢网络模型及其方法用于分析丁醇生产微生物的代谢特征和筛选减少目标
公开(公告)号：WO2009128644A3
公开(公告)日：2010-01-21
申请号：PCT/KR2009001923
申请日：2009-04-14
申请人： KOREA ADVANCED INST SCI & TECH , BIOFUELCHEM CO , GS CALTEX CORP , LEE SANG YUP , LEE JOUNG MIN , YUN HONG SEOK , JUNG KWANG SEOP
发明人： LEE SANG YUP , LEE JOUNG MIN , YUN HONG SEOK , JUNG KWANG SEOP
IPC分类号： C12Q1/32
CPC分类号： G01N33/569 , C12Q1/32 , C12Q1/527
摘要： The present invention relates to a metabolic network model for analysis of metabolic characteristics of butanol-producing microorganisms and methods using the same for analysis of metabolic characteristics of butanol-producing microorganisms and for screening deletion targets; and, more particularly, to establishing a metabolic network model of butanol-producing microorganisms using gene-protein-biochemical reaction associations and methods for analysis of metabolic characteristics such as a metabolic flow, etc., using the established model and for screening deletion targets with a simulation based on the metabolic flow analysis to improve the productivity of a specific metabolic product during the deletion. A metabolic network model according to the invention is useful for analysis of metabolic characteristics such as a metabolic flow, etc. of butanol-producing microorganisms and for screening a deletion target enzyme or a gene therefor to increase the production of a specific metabolic product. Moreover, unlike conventional methods, a deletion target screening method according to the invention makes it possible to predict deletion targets in an efficient manner, to save time and costs and to provide mutant microorganisms capable of producing a specific metabolic product at a high efficiency.
摘要翻译：本发明涉及用于分析丁醇生产微生物的代谢特征的代谢网络模型和使用其的丁醇生产微生物的代谢特征分析和筛选缺失目标的方法; 并且更具体地，涉及使用基因 - 蛋白质 - 生物化学反应关联建立丁醇生产微生物的代谢网络模型以及使用所建立的模型分析代谢流动等代谢特征的方法，并且用基于代谢流分析的模拟以提高缺失期间特定代谢产物的生产力。根据本发明的代谢网络模型可用于分析丁醇生产微生物的代谢特征如代谢流等，并用于筛选缺失目标酶或其基因以增加特定代谢产物的产生。此外，与常规方法不同，根据本发明的缺失靶标筛选方法使得有可能以有效的方式预测缺失靶标，节省时间和成本并且提供能够高效生产特定代谢产物的突变微生物。

2. 发明申请

WO2009128644A2 GENOME-SCALE BUTANOL-PRODUCING MICROORGANISM METABOLIC NETWORK MODEL AND METHODS USING THE SAME FOR ANALYSIS OF METABOLIC CHARACTERISTICS OF BUTANOL-PRODUCING MICROORGANISMS AND FOR SCREENING DELETION TARGETS 审中-公开
标题翻译：基因组大肠杆菌生产微生物代谢网络模型及其使用方法用于分析生产微生物的代谢特征和筛选目标的代谢特征
公开(公告)号：WO2009128644A2
公开(公告)日：2009-10-22
申请号：PCT/KR2009001923
申请日：2009-04-14
申请人： KOREA ADVANCED INST SCI & TECH , BIOFUELCHEM CO , GS CALTEX CORP , LEE SANG YUP , LEE JOUNG MIN , YUN HONG SEOK , JUNG KWANG SEOP
发明人： LEE SANG YUP , LEE JOUNG MIN , YUN HONG SEOK , JUNG KWANG SEOP
IPC分类号： C12Q1/32
CPC分类号： G01N33/569 , C12Q1/32 , C12Q1/527
摘要： The present invention relates to a metabolic network model for analysis of metabolic characteristics of butanol-producing microorganisms and methods using the same for analysis of metabolic characteristics of butanol-producing microorganisms and for screening deletion targets; and, more particularly, to establishing a metabolic network model of butanol-producing microorganisms using gene-protein-biochemical reaction associations and methods for analysis of metabolic characteristics such as a metabolic flow, etc., using the established model and for screening deletion targets with a simulation based on the metabolic flow analysis to improve the productivity of a specific metabolic product during the deletion. A metabolic network model according to the invention is useful for analysis of metabolic characteristics such as a metabolic flow, etc. of butanol-producing microorganisms and for screening a deletion target enzyme or a gene therefor to increase the production of a specific metabolic product. Moreover, unlike conventional methods, a deletion target screening method according to the invention makes it possible to predict deletion targets in an efficient manner, to save time and costs and to provide mutant microorganisms capable of producing a specific metabolic product at a high efficiency.
摘要翻译：本发明涉及用于分析产生丁醇的微生物的代谢特征的代谢网络模型，以及使用该代谢特征分析产生丁醇的微生物的代谢特征和筛选缺失目标的方法。更具体地，使用基因 - 蛋白质 - 生物化学反应关联建立丁醇生产微生物的代谢网络模型，以及使用建立的模型分析代谢流动等的代谢特征的方法，并用于筛选具有基于代谢流分析的模拟，以提高缺失期间特定代谢产物的生产力。根据本发明的代谢网络模型可用于分析产生丁醇的微生物的代谢流等的代谢特征，并且用于筛选缺失目标酶或其基因以增加特定代谢产物的产生。此外，与常规方法不同，根据本发明的缺失目标筛选方法使得有可能以有效的方式预测缺失目标，以节省时间和成本，并提供能够以高效率产生特定代谢产物的突变体微生物。

3. 发明申请

WO2011037415A3 RECOMBINANT MICROORGANISM WITH INCREASED BUTANOL PRODUCTION ABILITY, AND PREPARATION METHOD OF BUTANOL USING SAME 审中-公开
标题翻译：重组微生物提高丁醇生产能力，以及使用相同的丁醇制备方法
公开(公告)号：WO2011037415A3
公开(公告)日：2011-11-03
申请号：PCT/KR2010006511
申请日：2010-09-21
申请人： KOREA ADVANCED INST SCI & TECH , BIOFUELCHEM CO , GS CALTEX CORP , LEE SANG YUP , JANG YU-SIN , LEE JIN YOUNG , LEE JOUNG MIN , PARK JIN HWAN
发明人： LEE SANG YUP , JANG YU-SIN , LEE JIN YOUNG , LEE JOUNG MIN , PARK JIN HWAN
IPC分类号： C12N1/21 , C12N15/52 , C12P7/16
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to a recombinant microorganism with an increased butanol production ability, and a preparation method of butanol using the same, and more specifically, to a recombinant microorganism of which the butanol production ability is increased by the manipulation of metabolic pathways, and a preparation method of butanol using the same. The recombinant microorganism with an increased butanol production ability according to the present invention is characterized in that a gene encoding an enzyme which converts acetyl CoA into acetate is deleted in a host microorganism having a gene encoding enzymes involved in the biosynthetic pathway into acetyl CoA and butyryl CoA. Since the recombinant microorganism according to the present invention obtained by the manipulation of the metabolic flow of a microorganism can selectively produce butanol with a high efficiency, it is useful as a microorganism producing an industrial solvent and a transportation fuel.
摘要翻译：本发明涉及丁醇生产能力提高的重组微生物，以及使用其的丁醇的制备方法，更具体地说，涉及通过代谢途径的操作增加丁醇生产能力的重组微生物，以及丁醇的制备方法。根据本发明的具有增加的丁醇生产能力的重组微生物的特征在于编码将乙酰CoA转化为乙酸的酶的基因在具有编码参与生物合成途径的酶的基因的宿主微生物中缺失为乙酰辅酶A和丁酰基辅酶。由于通过操作微生物的代谢流而获得的根据本发明的重组微生物可以选择性地高效地生产丁醇，因此它可用作生产工业溶剂和运输燃料的微生物。

4. 发明申请

WO2009082148A3 ENHANCED ETHANOL AND BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING ETHANOL AND BUTANOL USING THE SAME 审中-公开
标题翻译：增强的乙醇和生成微生物的丁醇及使用该生物制备乙醇和丁醇的方法
公开(公告)号：WO2009082148A3
公开(公告)日：2009-09-24
申请号：PCT/KR2008007577
申请日：2008-12-22
申请人： KOREA ADVANCED INST SCI & TECH , BIOFUELCHEM CO , GS CALTEX CORP , LEE SANG YUP , JANG YU-SIN , LEE JIN YOUNG , JUNG KWANG SEOP , KIM JAE HYUN
发明人： LEE SANG YUP , JANG YU-SIN , LEE JIN YOUNG , JUNG KWANG SEOP , KIM JAE HYUN
IPC分类号： C12N15/09
CPC分类号： C12P7/065 , C12N9/0006 , C12N9/13 , C12P7/16 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to a recombinant microorganism having an enhanced ability to produce ethanol and butanol and a method for preparing ethanol and butanol using the same, and more particularly to a recombinant microorganism having an enhanced ability to produce ethanol and butanol, into which a gene encoding CoA transferase and a gene encoding alcohol/aldehyde dehydrogenase are introduced, and to a method for preparing ethanol and butanol using the same. The recombinant microorganism according to the present invention, obtained by manipulating metabolic pathways of microorganisms, is capable of producing butanol and ethanol exclusively without producing any byproduct, and thus is useful as a microorganism producing industrial solvents and transportation fuel.
摘要翻译：本发明涉及具有增强的乙醇和丁醇生产能力的重组微生物以及使用该微生物制备乙醇和丁醇的方法，并且更具体地涉及具有增强的乙醇和丁醇生产能力的重组微生物，其中基因编码CoA转移酶和编码醇/醛脱氢酶的基因的方法，以及使用该方法制备乙醇和丁醇的方法。通过操作微生物的代谢途径获得的根据本发明的重组微生物能够仅产生丁醇和乙醇而不产生任何副产物，因此可用作生产工业溶剂和运输燃料的微生物。

5. 发明申请

WO2011037414A3 RECOMBINANT MUTANT MICROORGANISM WITH INCREASED ALCOHOL PRODUCTION ABILITY, AND PREPARATION METHOD OF ALCOHOL USING SAME 审中-公开
标题翻译：具有提高的酒精生产能力的重组突变微生物和使用其的醇的制备方法
公开(公告)号：WO2011037414A3
公开(公告)日：2011-10-27
申请号：PCT/KR2010006510
申请日：2010-09-21
申请人： KOREA ADVANCED INST SCI & TECH , BIOFUELCHEM CO , GS CALTEX CORP , LEE SANG YUP , JANG YU-SIN , LEE JOUNG MIN , IM JUNG AE , SONG HYOHAK
发明人： LEE SANG YUP , JANG YU-SIN , LEE JOUNG MIN , IM JUNG AE , SONG HYOHAK
IPC分类号： C12N1/21 , C12N15/52 , C12P7/06 , C12P7/16 , C12R1/145
CPC分类号： C12N9/13 , C12N9/0006 , C12N9/88 , C12N15/74 , C12P3/00 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/26 , C12P7/28 , C12P7/52 , C12P7/54 , C12P7/56 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to a recombinant mutant microorganism with an increased alcohol production ability, and a preparation method of alcohol using the same, and more specifically, to a recombinant mutant microorganism which hardly produces acetone as a by-product and has an increased production ability of butanol, ethanol, isopropanol or an alcohol mixture capable of being used as fuel, and a preparation method of butanol, ethanol, isopropanol or an alcohol mixture using the same. The recombinant mutant microorganism with an increased production ability of butanol or an alcohol mixture and an acetone removing ability according to the present invention is characterized in that a gene encoding an enzyme involved in the production of butanol from butyryl-CoA or butylaldehyde and involved in the production of isopropanol from acetone is amplified or introduced into a host microorganism. Since the recombinant mutant microorganism according to the present invention hardly produces by-products such as acetone and the like by the manipulation of metabolic flow and can improve alcohol productivity, it is useful for the industrial production of butanol or an alcohol mixture comprising butanol and isopropanol.
摘要翻译：本发明涉及具有增加的醇生产能力的重组突变体微生物和使用其的醇的制备方法，更具体地，涉及难以产生丙酮作为副产物并且具有增加的生产能力的重组突变微生物丁醇，乙醇，异丙醇或可用作燃料的醇混合物，以及使用其的丁醇，乙醇，异丙醇或醇混合物的制备方法。根据本发明的丁醇或醇混合物的生产能力增加的重组突变微生物的特征在于编码参与从丁酰辅酶A或丁醛生产丁醇并涉及从丙酮生产异丙醇被扩增或引入宿主微生物。由于根据本发明的重组突变体微生物通过操作代谢流量几乎不产生诸如丙酮等副产物并且可以提高酒精生产率，因此可用于工业生产丁醇或包含丁醇和异丙醇的醇混合物。

6. 发明公开

EP2222845A4 ENHANCED ETHANOL AND BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING ETHANOL AND BUTANOL USING THE SAME 审中-公开
标题翻译：为了增加乙醇和丁醇的生产和方法微生物生产乙醇和丁醇SO
公开(公告)号：EP2222845A4
公开(公告)日：2012-03-07
申请号：EP08865113
申请日：2008-12-22
申请人： KOREA ADVANCED INST SCI & TECH , BIOFUELCHEM CO , GS CALTEX CORP
发明人： LEE SANG YUP , JANG YU-SIN , LEE JIN YOUNG , JUNG KWANG SEOP , KIM JAE HYUN
IPC分类号： C12N9/04 , C12N9/10 , C12P7/06 , C12P7/16
CPC分类号： C12P7/065 , C12N9/0006 , C12N9/13 , C12P7/16 , Y02E50/10 , Y02E50/17

7. 发明专利

AU2008341277A1 Enhanced ethanol and butanol producing microorganisms and method for preparing ethanol and butanol using the same 未知
公开(公告)号：AU2008341277A1
公开(公告)日：2009-07-02
申请号：AU2008341277
申请日：2008-12-22
申请人： BIOFUELCHEM CO , GS CALTEX CORP , KOREA ADVANCED INST SCI & TECH
发明人： JANG YU-SIN , LEE SANG YUP , KIM JAE HYUN , JUNG KWANG SEOP , LEE JIN YOUNG
IPC分类号： C12N15/09
摘要： The present invention relates to a recombinant microorganism having an enhanced ability to produce ethanol and butanol and a method for preparing ethanol and butanol using the same, and more particularly to a recombinant microorganism having an enhanced ability to produce ethanol and butanol, into which a gene encoding CoA transferase and a gene encoding alcohol/aldehyde dehydrogenase are introduced, and to a method for preparing ethanol and butanol using the same. The recombinant microorganism according to the present invention, obtained by manipulating metabolic pathways of microorganisms, is capable of producing butanol and ethanol exclusively without producing any byproduct, and thus is useful as a microorganism producing industrial solvents and transportation fuel.

8. 发明公开

EP2481793A4 RECOMBINANT MICROORGANISM WITH INCREASED BUTANOL PRODUCTION ABILITY, AND PREPARATION METHOD OF BUTANOL USING SAME 有权转让
标题翻译：随着对丁醇INCREASEDBUTANOLHERSTELLUNGSFÄHIGKEIT和方法重组微生物SO
公开(公告)号：EP2481793A4
公开(公告)日：2013-07-10
申请号：EP10819056
申请日：2010-09-21
申请人： KOREA ADVANCED INST SCI & TECH , BIOFUELCHEM CO , GS CALTEX CORP
发明人： LEE SANG YUP , JANG YU-SIN , LEE JIN YOUNG , LEE JOUNG MIN , PARK JIN HWAN
IPC分类号： C12N1/21 , C12N15/52 , C12P7/16
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及具有增加的生产丁醇的能力，并使用相同的生产丁醇的方法的重组微生物。更具体地，本发明涉及重组微生物谁产生丁醇能力通过他们的代谢网络的操作什么增加，并使用相同的生产丁醇的方法。具有增加的生产丁醇能力的重组微生物包含一个缺失的基因，酶的编码的并将乙酰辅酶A在宿主微生物于乙酸盐具有基因编码的酶在乙酰CoA并参与和丁酰辅酶A生物合成途径。通过操纵微生物的代谢通量得到的重组微生物能够选择性地产生丁醇具有高效率，并且因此作为用于生产工业溶剂和运输燃料的微生物有用。

9. 发明申请

WO2010093150A3 RECOMBINANT MICROORGANISM WITH ENHANCED ABILITY FOR GLYCEROL METABOLISM AND SUCCINIC ACID PRODUCTION, AND METHOD FOR PRODUCING SUCCINIC ACID USING SAME 审中-公开
标题翻译：具有增加甘油代谢和产酸生产能力的重组微生物，以及使用其制备磺酸的方法
公开(公告)号：WO2010093150A3
公开(公告)日：2010-12-09
申请号：PCT/KR2010000755
申请日：2010-02-08
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , JEON YONG JAE , SONG HYOHAK
发明人： LEE SANG YUP , JEON YONG JAE , SONG HYOHAK
IPC分类号： C12N15/31 , C12N1/21
CPC分类号： C12P7/46
摘要： The present invention relates to a recombinant microorganism with enhanced ability for glycerol metabolism and succinic acid production, and to a method for producing succinic acid using same. More particularly, the present invention relates to a recombinant microorganism which is enhanced to produce a high concentration succinic acid by effectively using inexpensive glycerol as a carbon source for cells. The recombinant microorganism according to the present invention enables succinic acid, which is a valuable substance produced by conventional chemical processes, to be produced through production-efficient and environmentally-friendly biological processes, thus reducing the costs of producing the target metabolite. Further, the recombinant microorganism according to the present invention is advantageous in that 100% pure succinic acid can be produced without forming byproducts, as opposed to conventional techniques in which only a slight reduction of byproducts such as acetic acid, lactic acid, formic acid, pyruvic acid, etc., is achieved.
摘要翻译：本发明涉及增加甘油代谢和琥珀酸生产能力的重组微生物，以及使用其生产琥珀酸的方法。更具体地，本发明涉及通过有效地使用廉价的甘油作为细胞的碳源来增强生产高浓度琥珀酸的重组微生物。根据本发明的重组微生物使得通过常规化学方法生产的有价值物质的琥珀酸通过生产有效和环境友好的生物过程产生，从而降低生产目标代谢物的成本。此外，根据本发明的重组微生物的优点在于，与常规技术相反，可以生产100％纯的琥珀酸而不形成副产物，其中只有轻微降低副产物如乙酸，乳酸，甲酸，丙酮酸等。

10. 发明申请

WO2009157702A3 METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF 审中-公开
标题翻译：使用具有增强聚氨酯的突变微生物制备聚乙烯和共聚物的方法，以及其生产能力的共聚物
公开(公告)号：WO2009157702A3
公开(公告)日：2010-04-22
申请号：PCT/KR2009003389
申请日：2009-06-24
申请人： LG CHEMICAL LTD , KOREA ADVANCED INST SCI & TECH , YANG TAEK HO , PARK SI JAE , KIM TAE WAN , LEE SANG YUP , JUNG YU KYUNG
发明人： YANG TAEK HO , PARK SI JAE , KIM TAE WAN , LEE SANG YUP , JUNG YU KYUNG
IPC分类号： C08G63/91
CPC分类号： C08G63/06 , C12P7/625 , C12P21/02
摘要： The present invention relates to a method for preparing a polylactate and a copolymer thereof using a mutant microorganism in which a gene involving a coenzyme A donor and lactate producing pathway is genetically manipulated to increase the productivity of the coenzyme A donor and lactate. According to the present invention, the amount of coenzyme A donor and the amount of lactate are simultaneously increased in a microbial metabolic pathway, to thereby enable an efficient biosynthesis of a polylactate and a hydroxyalkanoate-lactate copolymer containing a high content of lactate, thus making the method of the present invention industrially useful.
摘要翻译：本发明涉及一种使用突变微生物制备聚乳酸及其共聚物的方法，其中遗传操作涉及辅酶A供体和乳酸生产途径的基因以提高辅酶A供体和乳酸盐的生产率。根据本发明，在微生物代谢途径中同时增加辅酶A供体的量和乳酸的量，从而能够有效地生物合成含有高含量乳酸的聚乳酸和羟基链烷酸酯 - 乳酸共聚物，从而使本发明的方法在工业上有用。

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