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    • 4. 发明专利
    • PLANT WORKSHOP
    • JP2002058367A
    • 2002-02-26
    • JP2000247404
    • 2000-08-17
    • KANAI HIROAKIMIYATAKE KAZUTAKA
    • TAKIGAWA HISAYUKIMIYATAKE KAZUTAKA
    • A01G31/00
    • PROBLEM TO BE SOLVED: To reduce a waste liquid to zero by using a culture solution utilized for hydroponics for vegetables as a proliferation solution by combining cultivation of vegetables with proliferation of edible algae, to double an amount of production, to improve a rate of profit, to consume and to reduce carbon dioxide increased by facilities/a light source. SOLUTION: A cultivation tank for hydroponics of vegetables and ornamental flowering plants is installed in a complete control type cultivation chamber A by an artificial light source at a first floor and a cultivation water tank for hydroponics of edible algae is arranged in a sunshine utilizing cultivation chamber B at a second floor, respectively. Both the cultivation tanks of the cultivation chambers A and B are connected by a pipeline so as to send a culture solution in the culture tank to the proliferation water tank and a part of the pipeline is provided with a solution sending means equipped with a nutrient solution sterilization/analysis/adjustment function.
    • 6. 发明专利
    • PRODUCTION OF TREHALOSE
    • JPH0984593A
    • 1997-03-31
    • JP24428295
    • 1995-09-22
    • NAKANO OSAHISAIWASE COSFA KK
    • NAKANO OSAHISAMIYATAKE KAZUTAKATAKENAKA SHIGEOKONDO TOMOHIROKAWAURA SEIJIMATSUDA NORIO
    • C12P19/12C12R1/90
    • PROBLEM TO BE SOLVED: To produce a large amount of trehalose useful as a humectant for cosmetics at a low cost by culturing a protozoan belonging to the genus Euglena, placing the cultured microbial cell under salt stress, osmotic pressure stress, etc., and then extracting the trehalose from the microbial cell. SOLUTION: A protozoan belonging to the genus Euglena (e.g. Euglena gracilis Z strain) is cultured in a Koren-Hutner culture medium at 26 deg.C under irradiation of light, at 2000 luxes for 4days by shaking culture and the resultant protozoans of the genus Euglena are then recovered and suspended in a 15mM phosphoric acid buffer solution (pH6.8). Sodium chloride as a salt, stress loading agent is added and sorbitol and sucrose as osmotic pressure loading agent are respectively added so as to provide 50mM sorbitol and 50mM sucrose. Thereby, the protozoans are placed under the salt stress and/or the osmotic pressure stress to extract, the produced trehalose from the microbial cell in an 80% aqueous solution of ethanol. The prepared extract, solution is then evaporated to dryness. Thereby, a large amount, of the objective trehalose useful as a humectant, etc., of cosmetics is obtained at a low cost.