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1. 发明申请

WO2005030976A3 METHOD FOR PRODUCING A CAROTINOID-CONTAINING BIOMASS 审中-公开
标题翻译：生产含类奥生物的生物质的方法
公开(公告)号：WO2005030976A3
公开(公告)日：2005-09-29
申请号：PCT/DE2004002162
申请日：2004-09-26
申请人： KNOELL HANS FORSCHUNG EV , BIOSYNERGY GMBH , CHRISTNER ARNULF , MENZEL KLAUS-DIETER , CYRULIES MICHAEL , MENSCHEL CLAUDIA , KRETSCHMER ANDREAS , PAETZ REINHARD
发明人： CHRISTNER ARNULF , MENZEL KLAUS-DIETER , CYRULIES MICHAEL , MENSCHEL CLAUDIA , KRETSCHMER ANDREAS , PAETZ REINHARD
IPC分类号： C12P23/00 , C12R1/645
CPC分类号： C12P23/00
摘要： The invention relates to the production of a carotinoid-containing biomass. The aim of the invention is to provide a method for producing carotinoid-containing biomass that can be realized with a low level of complexity on a large scale and without antibiotics. To this end, the invention provides that non-genetically modified heterothallic fungi with an increased synthesis and storage capacity of ss-carotene are cultured in submerged cultures, whereby the biomass is propagated separately according to (+) and (-) pair type in multiple stages exclusively from spores. Biomass of the (+) pair type or culture liquid of the (+) pair type or homogenized biomass of the (+) pair type or, instead of the (+) pair type, gamone-type stimulators or lactones or trisporic acid or trisporic acid derivatives is/are added to the biomass of the (-) pair type after completion of the last biomass propagation phase before the production fermentation, and this submerged mixture is transferred into the nutrient solution of the product fermentation after a maturing time lasting form hours. The nutrient solution is composed in such a manner that at time tw, the growth phase of the biomass is terminated by phosphorous limitation, the synthesis of ss-carotene is additionally triggered by creating a short-term stress situation, and the solvent-free extraction of the ss-carotene from the resulting dry biomass is made possible by a phosphorous limitation during the growth phase.
摘要翻译：本发明涉及含类胡萝卜素的生物质的生产。指定的本发明的目的是提供用于制备类胡萝卜素的生物量的该aufwandtgering上的一大技术范围由不含抗生素来实现，通过与合成增加和β-胡萝卜素的存储容量的非遗传修饰的异宗金属蘑菇实现的过程生长在浸没培养物，其中所述生物质仅通过孢子（+）被分离的 - 和（ - ） - 交配型是在多个阶段增加，则生物量（ - ） - 交配型 - 的（+）的配对形式或培养液 - 配对类型的（+）的产物发酵的生物质之前的最后的生物量生长阶段完成之后或（+）的均质化的生物质 - 的配对形式或代替（+） - 交配型gamonartige刺激剂或内酯或trisporic或Trisporsäurederivate加入，并且每个这种浸没混合物被转移到在发酵产物的营养液几个小时老化期内，Nährl所述溶解组成为使得此时的生物质总重量由磷限制完成的生长期，β-胡萝卜素的合成附加地通过产生的短期应力状况，并通过在生长期磷限制触发，从β-胡萝卜素的无溶剂萃取允许产生的干生物质。

2. 发明专利

DE112004002328D2 未知
公开(公告)号：DE112004002328D2
公开(公告)日：2006-08-10
申请号：DE112004002328
申请日：2004-09-26
申请人： KNOELL HANS FORSCHUNG EV , BIOSYNERGY GMBH
发明人： MENSCHEL CLAUDIA , KRETSCHMER ANDREAS , PAETZ REINHARD , CHRISTNER ARNULF , MENZEL KLAUS-DIETER , CYRULIES MICHAEL
IPC分类号： C12P23/00

3. 发明专利

DE19845580A1 Preparation of madura hydroxylactone comprises cultivating Nonomuria rubra or derivatives and isolating product 未知
公开(公告)号：DE19845580A1
公开(公告)日：2000-04-06
申请号：DE19845580
申请日：1998-10-02
申请人： KNOELL HANS FORSCHUNG EV
发明人： ROTH MARTIN , PERLET KARIN , ROEMER ERNST , HILLIGER MATTHIAS , HEINISCH LOTHAR , MENZEL KLAUS-DIETER
IPC分类号： C12P7/66 , C12N1/20
摘要： Preparation of madura hydroxylactone (I) comprises cultivating Nonomuria rubra or its derivatives and isolating (I). An Independent claim is also included for Nonomuria rubra DSM 12263 and its derivatives.

4. 发明专利

DE19642105A1 未知
公开(公告)号：DE19642105A1
公开(公告)日：1998-04-16
申请号：DE19642105
申请日：1996-10-12
申请人： BUNA SOW LEUNA OLEFINVERB GMBH , KNOELL HANS FORSCHUNG EV
发明人： BERNDT CHRISTIANE , LEHMANN OLAF , MENZEL KLAUS-DIETER , DRIESCH DOMINIK , WULFES CHRISTIAN , GLIEM TORALF , CHRISTNER ARNULF DR , HILLIGER MATTHIAS DR , MIERSCH KARL DR
IPC分类号： C12M1/36 , C12N1/00 , C12N1/32 , C12N1/20
摘要： Methylotrophic micro-organisms are cultivated in known manner on the carbon substrate, methanol, using continuous measuring and secondary feeding processes in order to restrict the methanol concentration to very low values. According to the invention, a cultivation method comprising a plurality of cultivation stages is carried out in agitated and aerated bioreactors, the average methanol concentration decreasing from one stage to the next owing to suitable feeding methods. To that end, the methanol fed in during the cultivation stage (k-2) is proportional to the alkaline solution added in metered amounts during the regulation of the pH, in the cultivation stage (k-1), as the specific dosage, and in the final cultivation stage (k) first as the specific and then as the pO2-regulated dosage. This method prevents an accumulation of toxic intermediates and avoids the need to measure and regulate the methanol concentration during the entire cultivation period, such that costs are considerably reduced, in particular when the method is applied on an industrial scale.

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