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2. 发明授权

US07517647B2 Genetic screen for bioactive peptides 有权
标题翻译：生物活性肽的遗传筛选
公开(公告)号：US07517647B2
公开(公告)日：2009-04-14
申请号：US10953658
申请日：2004-09-29
申请人： Daniel B. Rifkin , John S. Munger , Justin P. Annes
发明人： Daniel B. Rifkin , John S. Munger , Justin P. Annes
IPC分类号： C12Q1/68 , C12Q1/66 , C12P21/02 , C12N5/10
CPC分类号： C12N15/1086 , C12N2502/99 , C12N2510/00 , C12N2799/027
摘要： A method of screening for a protein involved in the extracellular regulation of latent TGFβ activation by transducing a cell line with a retroviral cDNA library to create a reporter cell line that produces green fluorescent protein (GFP) in response to TGF-β signaling; growing individual clones created by the reporter cell line; co-culturing each individual clone with a second TGF-β reporter cell line that produces luciferase in response to TGF-β, wherein the luciferase production identifies positive clones; and identifying a mechanism of latent TGF-β activation that is employed by the positive clones. A TGFβ reporter cell line including a cell line; and a retroviral cDNA library, wherein the reporter cell line produces GFP in response to TGF-β signaling. A method of screening for gene products involved in a biological process.
摘要翻译：通过用逆转录病毒cDNA文库转导细胞系以产生响应于TGF-β信号产生绿色荧光蛋白（GFP）的报道细胞系，筛选涉及潜在TGFbeta活化的细胞外调节的蛋白质的方法; 记录细胞生成个体克隆; 每个单个克隆与用于响应于TGF-β产生荧光素酶的第二TGF-β报道细胞系共培养，其中荧光素酶产生鉴定阳性克隆; 并鉴定由阳性克隆使用的潜在TGF-β活化的机制。包含细胞系的TGFbeta报道细胞系; 和逆转录病毒cDNA文库，其中报道细胞系响应于TGF-β信号产生GFP。筛选参与生物过程的基因产物的方法。

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