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1. 发明授权

US5585271A Yeast agglutination genes and yeast containing them 失效
标题翻译：酵母凝集基因和酵母含有它们
公开(公告)号：US5585271A
公开(公告)日：1996-12-17
申请号：US325267
申请日：1994-11-18
申请人： Junji Watari , Yoshihiro Takata , Masahiro Ogawa , Merja Penttila , Maija-Leena Onnela , Sirkka Keranen
发明人： Junji Watari , Yoshihiro Takata , Masahiro Ogawa , Merja Penttila , Maija-Leena Onnela , Sirkka Keranen
IPC分类号： C12N15/09 , C07K14/395 , C12C11/00 , C12G1/022 , C12N1/19 , C12N5/10 , C12N15/31 , C12N15/81 , C12R1/865
CPC分类号： C12C12/004 , C07K14/395 , C12C12/006 , C12G1/0203 , C12N15/81
摘要： An agglutination gene of 4.7.+-.0.2 kb in yeast which codes for a polypeptide which exhibits agglutinative activity.
摘要翻译： PCT No.PCT / JP94 / 00290 Sec。 371日期：1994年11月18日 102（e）1994年11月18日PCT PCT 1994年2月24日PCT公布。出版物WO94 / 19475 日期1994年9月1日在编码显示凝集活性的多肽的酵母中的4.7 +/- 0.2kb的凝集基因。

2. 发明授权

US5866382A Xylose utilization by recombinant yeasts 失效
标题翻译：重组酵母的木糖利用率
公开(公告)号：US5866382A
公开(公告)日：1999-02-02
申请号：US336198
申请日：1994-11-03
申请人： Johan Hallborn , Merja Penttila , Heikki Ojamo , Mats Walfridsson , Ulla Airaksinen , Sirkka Keranen , Barbel Hahn-Hagerdal
发明人： Johan Hallborn , Merja Penttila , Heikki Ojamo , Mats Walfridsson , Ulla Airaksinen , Sirkka Keranen , Barbel Hahn-Hagerdal
IPC分类号： C12N1/15 , C12N1/19 , C12N9/02 , C12N9/04 , C12N15/52 , C12N15/81 , C12N15/90 , C12P7/06 , C12P7/18 , C12P7/40 , C12P19/02 , C12P7/02
CPC分类号： C12P19/02 , C12N15/52 , C12N15/81 , C12N15/905 , C12N9/0004 , C12N9/0006 , C12P7/06 , C12P7/18 , C12P7/40 , Y02E50/17 , Y02P20/582
摘要： This invention relates to recombinant-DNA-technology. Specifically, this invention relates to new recombinant yeast strains transformed with xylose reductase and/or xylitol dehydrogenase enzyme genes. A yeast strain transformed with the xylose reductase gene is capable of reducing xylose to xylitol and consequently of producing xylitol in vivo. If both of these genes are transformed into a yeast strain, the resultant strain is capable of producing ethanol on xylose containing medium during fermentation. Further, the said new yeast strains are capable of expressing the said two enzymes. Xylose reductase produced by these strains can be used in an enzymatic process for the production of xylitol in vitro.
摘要翻译：本发明涉及重组DNA技术。具体地，本发明涉及用木糖还原酶和/或木糖醇脱氢酶基因转化的新的重组酵母菌株。用木糖还原酶基因转化的酵母菌株能够将木糖还原成木糖醇，从而在体内产生木糖醇。如果将这两个基因转化成酵母菌株，则所得菌株能够在发酵期间在含有木糖的培养基上产生乙醇。此外，所述新酵母菌株能够表达所述两种酶。由这些菌株产生的木糖还原酶可用于体外生产木糖醇的酶法。

3. 发明授权

US5610034A Immunoglobulin production by trichoderma 失效
标题翻译：免疫球蛋白生产由木霉
公开(公告)号：US5610034A
公开(公告)日：1997-03-11
申请号：US756251
申请日：1991-08-30
申请人： Eini Nyyssonen , Sirkka Keranen , Merja Penttila , Kristiina Takkinen , Jonathan K.C. Knowles
发明人： Eini Nyyssonen , Sirkka Keranen , Merja Penttila , Kristiina Takkinen , Jonathan K.C. Knowles
IPC分类号： C07K14/37 , C07K16/00 , C12N9/64 , C12N15/80 , C12N15/13
CPC分类号： C12N9/6481 , C07K14/37 , C07K16/00 , C12N15/80
摘要： Methods for the production of recombinant immunoglobulins in a Trichoderma host are described.
摘要翻译：描述了在木霉属宿主中生产重组免疫球蛋白的方法。

4. 发明授权

US09238826B2 Method for producing terpenes 有权
标题翻译：生产萜烯的方法
公开(公告)号：US09238826B2
公开(公告)日：2016-01-19
申请号：US13883995
申请日：2011-11-14
申请人： Kirsi Bromann , Tina Nakari-Setala , Laura Ruohonen , Mervi Toivari , Merja Penttila
发明人： Kirsi Bromann , Tina Nakari-Setala , Laura Ruohonen , Mervi Toivari , Merja Penttila
IPC分类号： C07K14/37 , C12P5/00 , C07K14/38 , C12N15/52 , C12N15/80 , C12P15/00
CPC分类号： C12P5/007 , C07K14/37 , C07K14/38 , C12N15/52 , C12N15/80 , C12P15/00
摘要： The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene duster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use of Aspergillus nidulans FGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.
摘要翻译：本发明涉及一种在真菌中生产萜烯的方法，其中具有萜烯生物合成基因的萜烯生物合成基因簇和与所述基因可操作地连接的调节区被激活。本发明还涉及萜烯生物合成基因除尘器和这种萜烯生物合成基因簇的调节区域，可用于生产萜烯，使用调节剂来调节萜烯生产和使用构巢曲霉FGSC A4来生产萜烯。本发明的方法提供了较高收率的富含萜烯产物，而没有必需量的副产物。

5. 发明申请

US20070092956A1 Methods and materials for the production of organic products in cells of Candida species 审中-公开
标题翻译：在假丝酵母菌种细胞中生产有机产物的方法和材料
公开(公告)号：US20070092956A1
公开(公告)日：2007-04-26
申请号：US11507289
申请日：2006-08-21
申请人： Vineet Rajgarhia , Merja Penttila , Laura Ruohonen , Marja Ilmen , Kari Koivuranta , Pirkko Suominen
发明人： Vineet Rajgarhia , Merja Penttila , Laura Ruohonen , Marja Ilmen , Kari Koivuranta , Pirkko Suominen
IPC分类号： C12P7/56 , C12N1/18 , C12N15/74
CPC分类号： C12N15/81 , C12N9/0006 , C12N15/815 , C12P7/56
摘要： The present invention relates to biocatalysts that are cells, optimally of the Crabtree-negative phenotype, comprising expression vectors encoding genes heterologous to the cell that enable increased production of organic products. More specifically, the invention relates to genetically modified Candida cells, methods for making the Candida cells, and their use in production of organic products, particularly lactic acid.
摘要翻译：本发明涉及作为Crabtree阴性表型的细胞的生物催化剂，其包含编码与细胞异源的基因的表达载体，其能够增加有机产物的产生。更具体地，本发明涉及遗传修饰的念珠菌细胞，制备念珠菌细胞的方法，以及它们在生产有机产物，特别是乳酸中的用途。

6. 发明申请

US20050014204A1 Method for determining a gushing factor for a beverage 失效
标题翻译：用于确定饮料的涌流因子的方法
公开(公告)号：US20050014204A1
公开(公告)日：2005-01-20
申请号：US10920832
申请日：2004-08-18
申请人： Auli Haikara , Tuija Sarlin , Tiina Nakari-Setala , Merja Penttila
发明人： Auli Haikara , Tuija Sarlin , Tiina Nakari-Setala , Merja Penttila
IPC分类号： G01N33/569 , G01N33/53
CPC分类号： G01N33/56961 , G01N2333/37 , Y10S435/803 , Y10S435/812 , Y10S436/815
摘要： The invention relates to a method for determining a gushing factor for a beverage, in which method the quantity of hydrophobin is determined from the raw material of the beverage and/or from the beverage. The hydrophobin determination is performed immunologically using an immunological reaction between a hydrophobin antigen and an antibody.
摘要翻译：本发明涉及一种用于确定饮料的涌出因子的方法，其中疏水蛋白的量由饮料的原料和/或饮料确定。使用疏水蛋白抗原和抗体之间的免疫反应免疫进行疏水蛋白测定。

7. 发明申请

US20140045238A1 Method for producing terpenes 有权
标题翻译：生产萜烯的方法
公开(公告)号：US20140045238A1
公开(公告)日：2014-02-13
申请号：US13883995
申请日：2011-11-14
申请人： Kirsi Bromann , Tiina Nakari-Setala , Laura Ruohonen , Mervi Toivari , Merja Penttila
发明人： Kirsi Bromann , Tiina Nakari-Setala , Laura Ruohonen , Mervi Toivari , Merja Penttila
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C07K14/37 , C07K14/38 , C12N15/52 , C12N15/80 , C12P15/00
摘要： The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene cluster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use of Aspergillus nidulans FGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.
摘要翻译：本发明涉及一种在真菌中生产萜烯的方法，其中具有萜烯生物合成基因的萜烯生物合成基因簇和与所述基因可操作地连接的调节区被激活。本发明还涉及萜烯生物合成基因簇和这种萜烯生物合成基因簇的调节区域，可用于生产萜烯，使用调节剂调节萜烯生产和使用构巢曲霉FGSC A4来生产萜烯。本发明的方法提供了较高收率的富含萜烯产物，而没有必需量的副产物。

8. 发明申请

US20090226989A1 Genetically modified yeast of the species issatchenkia orientalis and closely relates species, and fermentation processes using same 有权
标题翻译：物种的转基因酵母和密切相关的物种，以及使用它们的发酵过程
公开(公告)号：US20090226989A1
公开(公告)日：2009-09-10
申请号：US11921161
申请日：2006-05-30
申请人： Pirkko Suominen , Aristos Aristidou , Merja Penttila , Marja Ilmen , Laura Ruohonen , Kari Koivuranta , Kevin Roberg-Perez
发明人： Pirkko Suominen , Aristos Aristidou , Merja Penttila , Marja Ilmen , Laura Ruohonen , Kari Koivuranta , Kevin Roberg-Perez
IPC分类号： C12P7/56 , C12N1/19 , C12N15/63
CPC分类号： C12P7/56 , C12N9/0006
摘要： Cells of the species Issatchenkia orientalis and closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.
摘要翻译：用载体转化东方蓟马和密切相关酵母菌种的细胞，引入外源性乳酸脱氢酶基因。细胞有效地产生乳酸并且在低pH，高乳酸盐滴度条件下具有抗性。

9. 发明授权

US07482144B2 Method for the production of xylitol 失效
标题翻译：生产木糖醇的方法
公开(公告)号：US07482144B2
公开(公告)日：2009-01-27
申请号：US10341220
申请日：2003-01-13
申请人： Heikki Ojamo , Merja Penttila , Heikki Heikkila , Jaana Uusitalo , Marja Ilmen , Marja-Leena Sarkki , Maija-Leena Vehkomaki
发明人： Heikki Ojamo , Merja Penttila , Heikki Heikkila , Jaana Uusitalo , Marja Ilmen , Marja-Leena Sarkki , Maija-Leena Vehkomaki
IPC分类号： C12P7/18 , C12N7/16 , C12N1/18
CPC分类号： C12P7/18
摘要： The invention relates to a method for the production of xylitol, the method comprising (a1) providing (i) a microorganism having xylanolytic activity, and (ii) a microorganism capable of converting a pentose sugar to xylitol; or (a2) providing a microorganism having xylanolytic activity and being capable of converting a pentose sugar to xylitol, (b) culturing the microorganism of step (a1) (i) or the microorganism of step (a2) in a medium comprising polymer or oligomer materials containing pentose sugars in conditions sufficient for enabling hydrolysis of said polymers or oligomers by the microorganism; (c) producing xylitol in the microorganism of step (a1) (ii) or in the microorganism of step (a2) by bioconversion of the hydrolysis products obtained in step (b), and (d) recovering said xylitol produced. The invention also relates to a microorganism, which has xylanolytic activity and has been genetically modified (i) to enhance its xylanolytic activity, and (ii) to reduce its xylitol metabolism.
摘要翻译：本发明涉及一种生产木糖醇的方法，所述方法包括（a1）提供（i）具有木聚糖分解活性的微生物和（ii）能够将戊糖转化成木糖醇的微生物; 或（a2）提供具有木聚糖分解活性并能够将戊糖转化为木糖醇的微生物，（b）在包含聚合物或低聚物的培养基中培养步骤（a1）（i）的微生物或步骤（a2）的微生物）在足以使微生物水解所述聚合物或低聚物的条件下含有戊糖的材料; （c）通过步骤（b）中获得的水解产物的生物转化和（d）回收所生产的木糖醇，在步骤（a1）（ⅱ）的微生物中或在步骤（a2）的微生物中生产木糖醇。本发明还涉及一种具有木聚糖分解活性且经遗传修饰的微生物（i）增强其木聚糖分解活性，和（ii）降低其木糖醇代谢。

10. 发明申请

US20050106734A1 Fungal micro-organism having an increased ability to carry out biotechnological process(es) 审中-公开
标题翻译：具有提高生物技术过程能力的真菌微生物
公开(公告)号：US20050106734A1
公开(公告)日：2005-05-19
申请号：US10494030
申请日：2002-10-29
申请人： Peter Richard , John Londesborough , Merja Penttila , Ritva Verho
发明人： Peter Richard , John Londesborough , Merja Penttila , Ritva Verho
IPC分类号： C12N15/09 , C12N1/14 , C12N1/19 , C12N9/02 , C12P7/06 , C12P7/62 , C12N1/18 , C12N15/74
CPC分类号： C12N9/0008 , Y02E50/17
摘要： The present invention relates to fungal microorganism having an increased ability to carry out biotechnological process(es). In particular, the invention relates to improving the regeneration of redox cofactors in biotechnological processes where useful products are produced from biomass containing pentoses. According to the invention, the microorganism is transformed with a DNA sequence encoding an NADP linked glyceraldehyde 3-phosphate dehydrogenase. The invention can be used to provide useful products for mankind from biological materials, including e.g. agricultural and forestry products, municipal waste. Examples of such useful products are ethanol, lactic acid, polyhydroxyalkanoates, amino acids, fats, vitamins, nucleotides and a wide variety of enzymes and pharmaceuticals.
摘要翻译：本发明涉及具有提高生物技术过程能力的真菌微生物。特别地，本发明涉及在生物技术过程中改进氧化还原辅因子的再生，其中有用产物由含有戊糖的生物质产生。根据本发明，用编码NADP连接的甘油醛-3-磷酸脱氢酶的DNA序列转化微生物。本发明可用于从生物材料为人类提供有用的产品，包括例如，农林产品，城市垃圾。这些有用产品的实例是乙醇，乳酸，聚羟基链烷酸酯，氨基酸，脂肪，维生素，核苷酸和多种酶和药物。

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