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1. 发明授权

US09448241B2 Mass spectrometry method for a polypeptide including a cysteine residue 有权
标题翻译：包含半胱氨酸残基的多肽的质谱法
公开(公告)号：US09448241B2
公开(公告)日：2016-09-20
申请号：US13576124
申请日：2011-03-28
申请人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
发明人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
IPC分类号： G01N33/68 , H01J49/00
CPC分类号： G01N33/6851 , H01J49/00 , Y10T436/182
摘要： The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.
摘要翻译：本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。提供了包括半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后使用带电粒子对基质处理的多肽进行解吸，用于质谱法。

2. 发明申请

US20120301968A1 MASS SPECTROMETRY METHOD 有权
标题翻译：质谱法
公开(公告)号：US20120301968A1
公开(公告)日：2012-11-29
申请号：US13576124
申请日：2011-03-28
申请人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
发明人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
IPC分类号： G01N27/62
CPC分类号： G01N33/6851 , H01J49/00 , Y10T436/182
摘要： The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.
摘要翻译：本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。提供了包括半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后使用带电粒子对基质处理的多肽进行解吸，用于质谱法。

3. 发明授权

US08415116B2 Method for pre-treating specimen and method for analyzing specimen 失效
公开(公告)号：US08415116B2
公开(公告)日：2013-04-09
申请号：US12113795
申请日：2008-05-01
申请人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu , Kazuhiro Ban
发明人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu , Kazuhiro Ban
IPC分类号： C12Q1/02 , G01N1/30 , G01N1/00
CPC分类号： G01N1/30 , G01N1/31 , G01N2001/282 , G01N2001/317
摘要： A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method.

4. 发明授权

US08084214B2 Treatment solution, method for pretreatment, method for acquiring information and method for detection 有权
标题翻译：治疗方案，预处理方法，获取信息的方法和检测方法
公开(公告)号：US08084214B2
公开(公告)日：2011-12-27
申请号：US12098822
申请日：2008-04-07
申请人： Hiroyuki Hashimoto , Manabu Komatsu , Satoru Hatakeyama
发明人： Hiroyuki Hashimoto , Manabu Komatsu , Satoru Hatakeyama
IPC分类号： G01N33/53
CPC分类号： G01N33/6848 , B82Y15/00 , G01N33/54313
摘要： The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens μm and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis.
摘要翻译：本发明旨在提供用于鉴定直径为10至几十μm的每个电池中所含的蛋白质的类型的高灵敏度方法以及所需的样品处理溶液。上述通过以下三个步骤实现：（1）用含有金颗粒和消化酶的水溶液处理患病组织的切片，并限制性地消化目的蛋白质，（2）测量片段肽的2维分布通过TOF-SIMS，以及（3）使用蛋白质组分析的结果并通过数值分析来显现病变组织切片中靶蛋白的二维分布。

5. 发明申请

US20110248156A1 INFORMATION ACQUIRING APPARATUS AND INFORMATION ACQUIRING METHOD FOR ACQUIRING MASS-RELATED INFORMATION 失效
标题翻译：获取信息的信息和获取质量相关信息的获取方法
公开(公告)号：US20110248156A1
公开(公告)日：2011-10-13
申请号：US13083510
申请日：2011-04-08
申请人： Manabu Komatsu , Hiroyuki Hashimoto
发明人： Manabu Komatsu , Hiroyuki Hashimoto
IPC分类号： H01J49/14 , H01J49/10
CPC分类号： H01J49/06 , H01J49/0463
摘要： Target molecules in a sample can be detected at an improved sensitivity by means of a mass spectrometer. A sample with or without a matrix is placed on a substrate and irradiated with a converged and pulsed primary beam selected from an ion beam, a neutral particle beam or a laser beam. Secondary ions and neutral molecules are emitted along with protons from the irradiated point of the sample as an electric field is applied between the substrate and an extraction electrode disposed above the substrate. A proton-control electrode is arranged in axial symmetry with the trajectory of the primary beam. A voltage is applied thereto so that the generated electric field decelerates the flying protons to raise their adhering efficiency to the flying neutral molecules.
摘要翻译：可以通过质谱仪以更高的灵敏度检测样品中的靶分子。将具有或不具有矩阵的样品放置在基底上并用从离子束，中性粒子束或激光束中选择的会聚且脉冲的一次束照射。随着电场施加在基板和设置在基板之上的引出电极之间，二次离子和中性分子与质子的辐射点一起被发射出来。质子控制电极与主光束的轨迹轴对称。施加电压使得所产生的电场使飞行质子减速，以提高它们对飞行中性分子的附着效率。

6. 发明申请

US20080179512A1 METHOD FOR OBTAINING INFORMATION AND DEVICE THEREFOR 有权
标题翻译：获取信息的方法及其设备
公开(公告)号：US20080179512A1
公开(公告)日：2008-07-31
申请号：US12017584
申请日：2008-01-22
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
IPC分类号： B01D59/44
CPC分类号： H01J49/142
摘要： A method for obtaining information on a mass of an object by time-of-flight mass spectrometry. This method includes placing colloidal metal particles for promoting ionization of the object inside the object at a depth ranging from 0.1 nm to 100 nm in opposition to a primary beam for the ionization; irradiating the object with the primary beam selected from the group of ions, neutral particles, and electrons, which can be focused, pulsed, and are capable of scanning, and laser beams, which can be focused, pulsed, and are capable of scanning to ionize a constituent of the object and to allow the ionized constituent to fly out of the object; and obtaining information on the mass of the flying constituent of the object by time-of-flight mass spectrometry.
摘要翻译：一种通过飞行时间质谱法获得物体质量信息的方法。该方法包括将胶体金属颗粒放置在与离子化的主光束相反的0.1nm至100nm范围内的物体内部物体的离子化; 用可以被聚焦，脉冲和能够扫描的离子，中性粒子和电子组中选择的主要束照射物体，以及可以被聚焦，脉冲且能够扫描的激光束电离物体的成分并使离子化成分从物体飞出; 并通过飞行时间质谱法获得关于物体飞行成分质量的信息。

7. 发明授权

US09086404B2 Method for treating living samples and analyzing the same 有权
标题翻译：处理活体样本并进行分析的方法
公开(公告)号：US09086404B2
公开(公告)日：2015-07-21
申请号：US11867276
申请日：2007-10-04
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Kazuhiro Ban , Yohei Murayama
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Kazuhiro Ban , Yohei Murayama
IPC分类号： G01N33/50 , G01N35/10
CPC分类号： G01N33/5082 , G01N35/1002 , G01N2035/1041
摘要： A method is provided by which a reagent is provided to a living sample, including contacting a thin-film containing a porous body with a living sample containing cells or tissues, and discharging a solution containing at least one or more reagents to a non-contact side of the thin-film by ink-jet to provide the solution to the specific area of the living sample through pores on the thin-film, wherein the smaller diameter of the pores on the porous body relative to the diameter of cells in the living sample enables the reagent to be provided to each individual cell separately. According to this method, the reagent and the reaction product can be fixed while maintaining positioning information of the target substance in the living sample, as a pretreatment for analyzing with high positioning accuracy the target substance in each individual cell in the living sample.
摘要翻译：提供了一种将试剂提供给活体样品的方法，包括使含有多孔体的薄膜与含有细胞或组织的活体样品接触，并将含有至少一种或多种试剂的溶液排出至非接触通过喷墨使薄膜的一侧通过薄膜上的孔提供到活体样品的特定区域的溶液，其中多孔体上的孔的直径相对于活体中细胞的直径较小样品使得可以分别向每个单独的细胞提供试剂。根据该方法，可以在维持活体样品中的目标物质的定位信息的同时固定试剂和反应产物，作为用于以高定位精度分析活体样品中各个细胞中的目标物质的预处理。

8. 发明申请

US20140087408A1 INFORMATION ACQUISITION METHOD 审中-公开
标题翻译：信息获取方法
公开(公告)号：US20140087408A1
公开(公告)日：2014-03-27
申请号：US13995645
申请日：2011-11-28
申请人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu
发明人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu
IPC分类号： G01N33/574
CPC分类号： G01N33/57415 , C07K14/4756 , G01N33/6848 , G01N2333/4756
摘要： The present invention relates to an information acquisition method of acquiring information of distribution of a protein or peptide in a sample based on mass information obtained by mass spectrometry of the protein or peptide. The method includes mass spectrometry of a definite region of the sample after limited proteolysis of the protein or peptide and acquisition of information relating to distribution using an ion peak that has a two-dimensional intensity distribution having a Pearson product-moment correlation coefficient of 0.5 to more and 1.0 or less in the definite region against the two-dimensional intensity distribution of the parent ion of the protein or the peptide subjected to the limited proteolysis and has a peak intensity ratio of larger than 1.0 against the peak intensity of the integrated spectrum of the parent ion in the definite region, wherein the m/z of the ion peak is greater than 500.
摘要翻译：本发明涉及基于通过蛋白质或肽的质谱法获得的质量信息获取样品中蛋白质或肽的分布信息的信息获取方法。该方法包括在蛋白质或肽的有限蛋白水解之后样品的确定区域的质谱，并且使用具有Pearson积矩相关系数为0.5的二维强度分布的离子峰获取与分布有关的信息相对于蛋白质或经受有限蛋白水解的肽的母离子的二维强度分布，确定区域中的1.0和更小，并且具有大于1.0的峰强度比与峰积分的峰强度比该定子区域中的母离子，其中离子峰的m / z大于500。

9. 发明授权

US08623656B2 Screening method for test specimen 失效
标题翻译：试样筛选方法
公开(公告)号：US08623656B2
公开(公告)日：2014-01-07
申请号：US13052272
申请日：2011-03-21
申请人： Manabu Komatsu , Hiroyuki Hashimoto
发明人： Manabu Komatsu , Hiroyuki Hashimoto
IPC分类号： G01N35/00 , G01N1/00 , G01N15/06 , G01N33/48
CPC分类号： H01J49/10 , B01L3/5085 , H01J49/40 , Y10T436/11 , Y10T436/112499 , Y10T436/25 , Y10T436/2575
摘要： A test specimen that has one or more chemical substances fixed to prescribed plural independent positions on a substrate, and the quantities of the chemical substances fixed at the respective prescribed positions are the total of integer multiples of existence quantity units defined for the respective chemical substances in the range from 1 amol to 1 nmol (excluding the case in which the total quantity is zero).
摘要翻译：将具有固定在基板上规定的多个独立位置的一种以上的化学物质的试样和固定在各规定位置的化学物质的量作为对各化学物质定义的存在量单位的整数倍的总和范围从1 amol到1 nmol（不包括总量为零的情况）。

10. 发明授权

US08436535B2 Information acquisition method, information acquisition apparatus and disease diagnosis method 有权
标题翻译：信息采集方法，信息获取装置及疾病诊断方法
公开(公告)号：US08436535B2
公开(公告)日：2013-05-07
申请号：US12712131
申请日：2010-02-24
申请人： Hiroyuki Hashimoto , Katsuaki Kuge , Manabu Komatsu , Kumi Nakamura , Kazuhiro Ban , Takeshi Imamura , Shin Kobayashi , Tadashi Okamoto
发明人： Hiroyuki Hashimoto , Katsuaki Kuge , Manabu Komatsu , Kumi Nakamura , Kazuhiro Ban , Takeshi Imamura , Shin Kobayashi , Tadashi Okamoto
IPC分类号： H01J17/26
CPC分类号： G01N33/6848 , G01N33/4833
摘要： An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry.
摘要翻译：一种用于获取关于目标对象的信息的信息获取方法，其包括使用促进所述目标对象的电离的物质促进所述目标对象的电离以使所述目标对象发射的步骤以及获取所述质量信息的步骤的飞行目标物体使用飞行时间二次离子质谱法。

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