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    • 1. 发明授权
    • Live-cell biosensor polypeptides and methods of use
    • 活细胞生物传感器多肽和使用方法
    • US07655434B2
    • 2010-02-02
    • US11052001
    • 2005-02-03
    • Joshua T. JonesAngela Teresa HahnTobias Meyer
    • Joshua T. JonesAngela Teresa HahnTobias Meyer
    • C12Q1/02C12N5/06
    • G01N33/502G01N33/5008G01N33/582
    • Methods and compositions for determining the duration of a cell cycle phase of a mammalian cell, as well as identification of the cell cycle stage of fixed mammalian cells, are provided. In practicing the subject methods, at least one biosensor polypeptide that monitors a cell-cycle phase in a mammalian cell, such as mitosis, G1, S, or G2 phase, is used to determine the duration of a cell-cycle phase of a mammalian cell. Also provided are methods for identifying an agent (e.g., a gene product or small molecule compound) that modulates the duration of a cell-cycle phase of a mammalian cell, as well as kits and systems for practicing the subject methods.
    • 提供了用于确定哺乳动物细胞的细胞周期阶段的持续时间的方法和组合物,以及固定哺乳动物细胞的细胞周期阶段的鉴定。 在实施本发明方法中,使用至少一种监测哺乳动物细胞(例如有丝分裂,G1,S或G2期)中的细胞周期相的生物传感器多肽来确定哺乳动物的细胞周期阶段的持续时间 细胞。 还提供了用于鉴定调节哺乳动物细胞的细胞周期阶段的持续时间的试剂(例如,基因产物或小分子化合物)以及用于实践本发明方法的试剂盒和系统的方法。