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1. 发明授权

US07095502B2 Optical structures for metal-enhanced sensing 有权
标题翻译：用于金属增强感测的光学结构
公开(公告)号：US07095502B2
公开(公告)日：2006-08-22
申请号：US10633715
申请日：2003-08-05
申请人： Joseph Lakowicz , Zygmunt Gryczynski , Chris Geddes
发明人： Joseph Lakowicz , Zygmunt Gryczynski , Chris Geddes
IPC分类号： G01N21/55 , G01N1/10
CPC分类号： G01N21/648
摘要： The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.
摘要翻译：本发明涉及使用金属增强荧光检测分子的荧光的光学结构和方法。特别地，本发明描述了使用表面等离激元激发来激发金属表面附近的荧光团，并且通过耦合到等离子体共振并引导到检测器来有效地收集发射。更具体地，本发明利用等离子体激发的荧光信号的独特方向性。本发明涉及使用金属增强荧光的光学结构，包括：具有导电外涂层的光纤; 具有在前端表面上具有弯曲侧面的圆锥形凹陷的发光二极管（LED），所述弯曲侧在所述外表面上具有相对于所述LED的导电涂层。

2. 发明授权

US07400397B2 Optical structures for metal-enhanced sensing 有权
标题翻译：用于金属增强感测的光学结构
公开(公告)号：US07400397B2
公开(公告)日：2008-07-15
申请号：US11488001
申请日：2006-07-18
申请人： Joseph Lakowicz , Zygmunt Gryczynski , Chris Geddes
发明人： Joseph Lakowicz , Zygmunt Gryczynski , Chris Geddes
IPC分类号： G01J3/30
CPC分类号： G01N21/648
摘要： The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.
摘要翻译：本发明涉及使用金属增强荧光检测分子的荧光的光学结构和方法。特别地，本发明描述了使用表面等离激元激发来激发金属表面附近的荧光团，并且通过耦合到等离子体共振并引导到检测器来有效地收集发射。更具体地，本发明利用等离子体激发的荧光信号的独特方向性。本发明涉及使用金属增强荧光的光学结构，包括：具有导电外涂层的光纤; 具有在前端表面上具有弯曲侧面的圆锥形凹陷的发光二极管（LED），所述弯曲侧在所述外表面上具有相对于所述LED的导电涂层。

3. 发明申请

US20050053974A1 Apparatus and methods for surface plasmon-coupled directional emission 审中-公开
标题翻译：表面等离子体耦合定向发射的装置和方法
公开(公告)号：US20050053974A1
公开(公告)日：2005-03-10
申请号：US10849465
申请日：2004-05-20
申请人： Joseph Lakowicz , Joanna Malicka , Ignacy Gryczynski , Zygmunt Gryczynski
发明人： Joseph Lakowicz , Joanna Malicka , Ignacy Gryczynski , Zygmunt Gryczynski
IPC分类号： C12Q1/68 , G01N21/55 , G01N21/64 , G01N21/76
CPC分类号： G01N21/6428 , G01N21/4788 , G01N21/6452 , G01N21/648 , G01N21/76 , G01N33/54373 , G01N2021/6432
摘要： Methods and apparatus for fluorescence detection which can increase sensitivity by as much as 20 to 1000-fold are described. This method can preferably also decrease the contribution of sample autofluorescence to the detected signal. The method uses coupling of excited fluorophores with the surface plasmon resonance present in thin conductive films, for example silver, gold, aluminum, copper, or the like. The phenomenon of surface plasmon-coupled emission (SPCE) occurs for fluorophores in a volume adjacent to the conductive layer. This interaction is independent of the mode of excitation, that is, does not require evanescent wave or surface-plasmon excitation. However, such modes of excitation can be advantageous. SPCE can occur over a narrow angular distribution, converting normally isotropic emission into easily collected directional emission. In preferred embodiments, up to 50% of the emission from unoriented samples can be collected, usually much more than typical fluorescence collection efficiencies, which can be 1% or less. Examples are presented showing how simple optical configurations can be used in diagnostics, sensing, or biotechnology applications. Surface plasmon-coupled emission is likely to find widespread applications throughout the biosciences.
摘要翻译：描述了将灵敏度提高多达20到1000倍的用于荧光检测的方法和装置。该方法也可以优选地降低样品自发荧光对检测信号的贡献。该方法使用激发的荧光团与存在于薄导电膜（例如银，金，铝，铜等）中的表面等离子体共振的耦合。在与导电层相邻的体积中的荧光团发生表面等离激元耦合发射（SPCE）的现象。这种相互作用与激发模式无关，也就是说，不需要ev逝波或表面等离子体激发。然而，这种激发模式可能是有利的。 SPCE可以发生在窄角分布上，将正常的各向同性发射转换成易于收集的定向发射。在优选的实施方案中，可以收集来自无取向样品的高达50％的发射，通常比典型的荧光收集效率高得多，其可以为1％或更少。现在举例说明如何在诊断，感测或生物技术应用中使用简单的光学配置。表面等离子体耦合发射很可能在整个生物科学领域得到广泛的应用。

4. 发明申请

US20050031536A1 Methods and compositions comprising monitoring devices 审中-公开
标题翻译：包括监测装置的方法和组合物
公开(公告)号：US20050031536A1
公开(公告)日：2005-02-10
申请号：US10886388
申请日：2004-07-07
申请人： Zygmunt Gryczynski , Candace McCombs , Joseph Lakowicz , Omoefe Abugo
发明人： Zygmunt Gryczynski , Candace McCombs , Joseph Lakowicz , Omoefe Abugo
IPC分类号： A61K49/00 , G06F19/00
CPC分类号： G06F19/3456 , A61B5/0071 , A61B5/4833 , A61B5/681 , A61K49/0034 , A61K49/0041 , A61K49/0063 , A61K49/0082 , Y02A90/26
摘要： Methods and compositions for the detection and monitoring of drug therapy are provided. In particular, efficient and sensitive methods for the detection of drug ingestion are provided for determining whether individuals are complying with prescribed therapeutic regimens, and for providing a mechanism for identifying drug-resistant strains of infectious agents. The claimed methods and compositions involve the application of transdermal devices containing detection mechanisms for receiving and recording signals generated by the ingestion of a labeled drug. Such devices are attached to the skin for the duration of drug therapy and compliance is determined either by direct reading, or by remote monitoring whereby signals are transmitted from the device and received at an external site such as a healthcare facility.
摘要翻译：提供了用于检测和监测药物治疗的方法和组合物。特别地，提供用于检测药物摄入的有效和敏感的方法用于确定个体是否符合规定的治疗方案，以及提供用于鉴定感染性药物的耐药菌株的机制。所要求保护的方法和组合物涉及应用含有检测机制的透皮装置，用于接收和记录由摄取标记药物产生的信号。这样的装置在药物治疗期间附着到皮肤，并且通过直接读取或通过远程监测来确定顺应性，其中信号从装置传输并在诸如医疗保健设施的外部部位接收。

5. 发明申请

US20070020182A1 Quaternary nitrogen heterocyclic compounds for detecting aqueous monosaccharides in physiological fluids 有权
标题翻译：用于检测生理液体中单糖水溶液的四元氮杂环化合物
公开(公告)号：US20070020182A1
公开(公告)日：2007-01-25
申请号：US11318663
申请日：2005-12-27
申请人： Chris Geddes , Ramachandram Badugu , Joseph Lakowicz
发明人： Chris Geddes , Ramachandram Badugu , Joseph Lakowicz
IPC分类号： A61K49/00 , C07F5/02 , G01N33/00
CPC分类号： C07F5/025 , A61K49/0021 , Y10T436/143333 , Y10T436/144444 , Y10T436/193333
摘要： Quaternary nitrogen heterocyclic boronic acid-containing compounds are described, which are sensitive to glucose and fructose, as well as a variety of other physiologically important analytes, such as aqueous chloride and iodide, and a method of using the compounds. Also disclosed is a contact lens doped with the quaternary nitrogen heterocyclic boronic acid-containing compound, and a method of using the doped contact lens to measure the concentration of analyte in tears under physiological conditions.
摘要翻译：描述了对葡萄糖和果糖敏感的第四季氮杂环硼酸化合物，以及各种其它生理重要的分析物，例如氯化物和碘化物，以及使用该化合物的方法。还公开了掺杂有季氮杂环硼酸的化合物的隐形眼镜，以及使用掺杂隐形眼镜在生理条件下测量泪液浓度的方法。

6. 发明申请

US20060256331A1 Optical structures for metal-enhanced sensing 有权
公开(公告)号：US20060256331A1
公开(公告)日：2006-11-16
申请号：US11488001
申请日：2006-07-18
申请人： Joseph Lakowicz , Zygmuni Gryczynski , Chris Geddes
发明人： Joseph Lakowicz , Zygmuni Gryczynski , Chris Geddes
IPC分类号： G01J3/30 , G01N21/64
CPC分类号： G01N21/648
摘要： The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.

7. 发明申请

US20140256593A1 PLASMONIC SUBSTRATES FOR METAL-ENHANCED FLUORESCENCE BASED SENSING, IMAGING AND ASSAYS 审中-公开
标题翻译：用于基于金属增强荧光的感光，成像和测量的等离子体基底
公开(公告)号：US20140256593A1
公开(公告)日：2014-09-11
申请号：US14359343
申请日：2012-11-23
申请人： Henryk Szmacinski , Joseph Lakowicz
发明人： Henryk Szmacinski , Joseph Lakowicz
IPC分类号： G01N33/543 , G01N33/68 , G01N33/569 , G02B5/00
CPC分类号： G01N33/54373 , B82Y15/00 , B82Y20/00 , C12Q1/02 , G01N21/554 , G01N33/54346 , G01N33/56972 , G01N33/6863 , G02B5/008 , Y10S977/773
摘要： Techniques for metal enhanced fluorescence include determining a calibration curve that relates concentration of a particular analyte to at least one of intensity or lifetime of fluorescent emissions at a functionalized substrate in response to incident light, for a plurality of known concentrations of the particular analyte mixed with a reagent. The functionalized substrate comprises a plasmonic substrate and a bioactive target molecule that has an affinity for the particular analyte. The reagent comprises a detection molecule. A concentration of the particular analyte in a sample is determined directly from the calibration curve and measurements, in response to the incident light, of at least one of intensity or lifetime of fluorescent emissions at the functionalized substrate in contact with the sample and reagent.
摘要翻译：用于金属增强荧光的技术包括：确定与特定分析物的浓度相关的功能化底物的荧光发射的强度或寿命中的至少一个响应于入射光，将多个已知浓度的特定分析物与试剂。官能化底物包含等离激元底物和对特定分析物具有亲和力的生物活性靶分子。试剂包括检测分子。直接从校准曲线确定样品中特定分析物的浓度，并响应于入射光，测量在与样品和试剂接触的官能化底物处的荧光发射的强度或寿命中的至少一个。

8. 发明申请

US20050202464A1 Radiative decay engineering 有权
标题翻译：辐射衰变工程
公开(公告)号：US20050202464A1
公开(公告)日：2005-09-15
申请号：US10990549
申请日：2004-11-18
申请人： Joseph Lakowicz
发明人： Joseph Lakowicz
IPC分类号： G01N33/53 , C12M1/00 , C12Q1/68 , G01N21/78 , G01N33/542 , G01N33/553 , G01N33/58 , G01N37/00
CPC分类号： C12Q1/6818 , C12Q1/682 , Y10S436/805 , Y10T436/143333 , C12Q2563/143 , C12Q2563/149 , C12Q2563/107
摘要： Compositions and methods for increasing the fluorescence intensity of molecules are provided. In particular, compositions and methods directed to increasing the intrinsic fluorescence of biomolecules and low quantum yield fluorophores are described. The intrinsic fluorescence of biomolecules is increased by positioning a metal particle and a biomolecule at a distance apart sufficient to increase the radiative decay rate of the biomolecule. Methods for the identification of nucleic acids are also provided. The compositions and methods can also be used to increase the emission of any fluorophore, such as the extrinsic probes used to label biomolecules.
摘要翻译：提供了增加分子荧光强度的组合物和方法。特别地，描述了用于增加生物分子和低量子产率荧光团的固有荧光的组合物和方法。通过将金属颗粒和生物分子定位在足以增加生物分子的辐射衰减速率的距离处，生物分子的固有荧光增加。还提供了用于鉴定核酸的方法。组合物和方法也可用于增加任何荧光团的发射，例如用于标记生物分子的外在探针。

9. 发明申请

US20050158245A1 Fluorescent probes for saccharrides 失效
标题翻译：糖类荧光探针
公开(公告)号：US20050158245A1
公开(公告)日：2005-07-21
申请号：US11075817
申请日：2005-03-10
申请人： Joseph Lakowicz , Nicolas Dicesare
发明人： Joseph Lakowicz , Nicolas Dicesare
IPC分类号： A61B5/00 , C07F5/02 , G01N31/22 , A61K49/00 , G01N33/00
CPC分类号： G01N31/22 , A61B5/14532 , C07F5/025 , Y10T436/143333 , Y10T436/144444 , Y10T436/203332
摘要： The spectroscopic and photophysical properties of fluorescent probes comprising donor-acceptor derivatives comprising the boric acid group or a derivative of boric acid, B(OH)3 (or borate ion, BO(OH)2−1), arsenious acid, H3 AsO3 (or arsenite ion, H2AsO3−1), telluric acid, H6TeO6 (or tellurate ion, H5 TeO6−1) or germanic acid, Ge(OH)6 (or germanate ion, GeO(OH)3−1) are described. Method of using said probes are also provided.
摘要翻译：包含硼酸基或硼酸衍生物B（OH）3（或硼酸根离子，BO（OH）2）的供体 - 受体衍生物的荧光探针的光谱和光物理性质亚砷酸，H 3 AsO 3（或亚砷酸根离子，H 2 O 3）碲酸，H 6 TeO 6（或碲酸根离子，H 2 O 3， Ge（OH）6（或锗酸根离子，GeO（OH）3），或锗酸根离子，GeO（OH） SUB> 3 ^{-1 ）。还提供了使用所述探针的方法。}

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