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1. 发明授权

US07186891B1 Plant cells and plants expressing chimeric isoprenoid synthases 有权
标题翻译：表达嵌合型类异戊二烯合酶的植物细胞和植物
公开(公告)号：US07186891B1
公开(公告)日：2007-03-06
申请号：US09514513
申请日：2000-02-28
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N9/88 , C12N9/00 , C12N15/8243
摘要： The invention features plant cells and plants that include a nucleic acid molecule encoding a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned homologous domain. The chimeric isoprenoid synthases of the invention catalyze the production of isoprenoid reaction products that are not produced when the asymmetrically positioned homologous domain is positioned at its naturally-occurring site in an isoprenoid synthase polypeptide.
摘要翻译：本发明的特征在于植物细胞和植物，其包括编码嵌合型类异戊二烯合酶多肽的核酸分子，其包括不对称定位的同源结构域。本发明的嵌合类异戊二烯合酶催化产生类异戊二烯反应产物，当不对称定位的同源结构域位于其在异戊二烯合酶多肽中的天然存在位点时不产生。

2. 发明授权

US5824774A Chimeric isoprenoid synthases and uses thereof 失效
标题翻译：嵌合类异戊二烯合酶及其用途
公开(公告)号：US5824774A
公开(公告)日：1998-10-20
申请号：US631341
申请日：1996-04-12
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： A61K38/00 , C12N15/09 , A01N63/00 , A61K31/00 , A61K31/01 , A61K31/015 , A61P31/00 , A61P31/04 , A61P31/10 , A61P35/00 , C07H21/00 , C07K1/00 , C07K2/00 , C07K4/00 , C07K5/00 , C07K7/00 , C07K14/00 , C07K16/00 , C07K17/00 , C12N1/21 , C12N9/00 , C12N9/88 , C12N15/60 , C12N15/82 , C12R1/19
CPC分类号： C12N9/00 , C12N15/8243 , C12N9/88 , C07K2319/00
摘要： Disclosed is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous isoprenoid synthase. Also disclosed is a chimeric isoprenoid synthase polypeptide including an assymetrically positioned homologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.
摘要翻译：本发明公开了一种嵌合型类异戊二烯合酶，其包括第一种类型的类异戊二烯合酶的第一结构域，其与第二异源类异戊二烯合酶连接到第二结构域，由此嵌合型类异戊二烯合酶能够催化不产生的类异戊二烯反应产物的产生不存在第二种异源类异戊二烯合酶的第二结构域。还公开了嵌合型类异戊二烯合酶多肽，其包括非均衡定位的同源结构域，其中嵌合型类异戊二烯合酶能够催化当该结构域位于其类固醇合成酶多肽的天然存在位点时不产生的类异戊二烯反应产物的产生。

3. 发明授权

US08354504B2 Chimeric isoprenoid synthases and uses thereof 有权
标题翻译：嵌合类异戊二烯合酶及其用途
公开(公告)号：US08354504B2
公开(公告)日：2013-01-15
申请号：US12927805
申请日：2010-11-23
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： C07K14/415
CPC分类号： C12P5/007 , C07K2319/00 , C12N9/00 , C12N9/1003 , C12N9/88 , C12N15/8243
摘要： Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.
摘要翻译：本发明提供了一种嵌合型类异戊二烯合酶，其包含与第二种异戊二烯合成酶连接到第二结构域的第一种类型的类异戊二烯合酶的第一结构域，其中嵌合型类异戊二烯合酶能够催化不产生的类异戊二烯反应产物不存在第二种异源的类异戊二烯合酶的第二结构域。还提供了嵌合型类异戊二烯合酶多肽，其包括不对称定位的异源结构域，由此嵌合型类异戊二烯合酶能够催化当该结构域位于类固醇合成酶多肽的天然存在位点时不产生的类异戊二烯反应产物的产生。

4. 发明授权

US08106260B2 Chimeric isoprenoid synthases and uses thereof 有权
标题翻译：嵌合类异戊二烯合酶及其用途
公开(公告)号：US08106260B2
公开(公告)日：2012-01-31
申请号：US10717500
申请日：2003-11-21
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： C12N15/29 , C12N15/52 , C12N15/82 , A01H5/00
CPC分类号： C12P5/007 , C07K2319/00 , C12N9/00 , C12N9/1003 , C12N9/88 , C12N15/8243
摘要： Disclosed is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous isoprenoid synthase. Also disclosed is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned homologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.
摘要翻译：本发明公开了一种嵌合型类异戊二烯合酶，其包括第一种类型的类异戊二烯合酶的第一结构域，其与第二异源类异戊二烯合酶连接到第二结构域，由此嵌合型类异戊二烯合酶能够催化不产生的类异戊二烯反应产物的产生不存在第二种异源类异戊二烯合酶的第二结构域。还公开了嵌合型类异戊二烯合酶多肽，其包括不对称定位的同源结构域，其中嵌合型类异戊二烯合酶能够催化产生类异戊二烯反应产物，当该结构域位于其在类异戊二烯合酶多肽的天然存在位点时不产生。

5. 发明申请

US20130071877A1 Chimeric isoprenoid synthases and uses thereof 失效
公开(公告)号：US20130071877A1
公开(公告)日：2013-03-21
申请号：US13694098
申请日：2012-10-26
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： C12N9/88
CPC分类号： C12P5/007 , C07K2319/00 , C12N9/00 , C12N9/1003 , C12N9/88 , C12N15/8243
摘要： Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.

6. 发明申请

US20110081703A1 Chimeric isoprenoid synthases and uses thereof 有权
标题翻译：嵌合类异戊二烯合酶及其用途
公开(公告)号：US20110081703A1
公开(公告)日：2011-04-07
申请号：US12927805
申请日：2010-11-23
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： C12N9/10 , C07H21/04 , C12N15/63 , C12N1/21 , C12N1/19 , C12N1/15 , C12N5/10 , A01H5/00
CPC分类号： C12P5/007 , C07K2319/00 , C12N9/00 , C12N9/1003 , C12N9/88 , C12N15/8243
摘要： Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.
摘要翻译：本发明提供了一种嵌合型类异戊二烯合酶，其包含与第二种异戊二烯合成酶连接到第二结构域的第一种类型的类异戊二烯合酶的第一结构域，其中嵌合型类异戊二烯合酶能够催化不产生的类异戊二烯反应产物不存在第二种异源的类异戊二烯合酶的第二结构域。还提供了嵌合型类异戊二烯合酶多肽，其包括不对称定位的异源结构域，由此嵌合型类异戊二烯合酶能够催化当该结构域位于类固醇合成酶多肽的天然存在位点时不产生的类异戊二烯反应产物的产生。

7. 发明申请

US20140242660A1 Chimeric isoprenoid synthases and uses thereof 审中-公开
标题翻译：嵌合类异戊二烯合酶及其用途
公开(公告)号：US20140242660A1
公开(公告)日：2014-08-28
申请号：US14120177
申请日：2014-05-02
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： C12P5/00 , C12N9/10
CPC分类号： C12P5/007 , C07K2319/00 , C12N9/00 , C12N9/1003 , C12N9/88 , C12N15/8243
摘要： Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.
摘要翻译：本发明提供了一种嵌合型类异戊二烯合酶，其包含与第二种异戊二烯合成酶连接到第二结构域的第一种类型的类异戊二烯合酶的第一结构域，其中嵌合型类异戊二烯合酶能够催化不产生的类异戊二烯反应产物不存在第二种异源的类异戊二烯合酶的第二结构域。还提供了嵌合型类异戊二烯合酶多肽，其包括不对称定位的异源结构域，由此嵌合型类异戊二烯合酶能够催化当该结构域位于类固醇合成酶多肽的天然存在位点时不产生的类异戊二烯反应产物的产生。

8. 发明授权

US08741651B2 Chimeric isoprenoid synthases and uses thereof 失效
公开(公告)号：US08741651B2
公开(公告)日：2014-06-03
申请号：US13694098
申请日：2012-10-26
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： C12N15/29 , C12N15/52 , C12N15/82
CPC分类号： C12P5/007 , C07K2319/00 , C12N9/00 , C12N9/1003 , C12N9/88 , C12N15/8243
摘要： Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.

9. 发明授权

US06072045A Chimeric isoprenoid synthases and uses thereof 有权
标题翻译：嵌合类异戊二烯合酶及其用途
公开(公告)号：US06072045A
公开(公告)日：2000-06-06
申请号：US134699
申请日：1998-08-14
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
IPC分类号： A61K38/00 , C12N15/09 , A01N63/00 , A61K31/00 , A61K31/01 , A61K31/015 , A61P31/00 , A61P31/04 , A61P31/10 , A61P35/00 , C07H21/00 , C07K1/00 , C07K2/00 , C07K4/00 , C07K5/00 , C07K7/00 , C07K14/00 , C07K16/00 , C07K17/00 , C12N1/21 , C12N9/00 , C12N9/88 , C12N15/60 , C12N15/82 , C12R1/19 , C07H21/02
CPC分类号： C12N9/00 , C12N15/8243 , C12N9/88 , C07K2319/00
摘要： Disclosed is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous isoprenoid synthase. Also disclosed is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned homologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.
摘要翻译：本发明公开了一种嵌合型类异戊二烯合酶，其包括第一种类型的类异戊二烯合酶的第一结构域，其与第二异源类异戊二烯合酶连接到第二结构域，由此嵌合型类异戊二烯合酶能够催化不产生的类异戊二烯反应产物的产生不存在第二种异源类异戊二烯合酶的第二结构域。还公开了嵌合型类异戊二烯合酶多肽，其包括不对称定位的同源结构域，其中嵌合型类异戊二烯合酶能够催化产生类异戊二烯反应产物，当该结构域位于其在类异戊二烯合酶多肽的天然存在位点时不产生。

10. 发明申请

US20140356926A1 Novel sesquiterpene synthase gene and protein 有权
标题翻译：新型倍半萜合酶基因和蛋白质
公开(公告)号：US20140356926A1
公开(公告)日：2014-12-04
申请号：US14121245
申请日：2014-08-13
申请人： Joseph Chappell , Bryan Greenhagen
发明人： Joseph Chappell , Bryan Greenhagen
IPC分类号： C12P5/00 , C12N9/88
CPC分类号： C12P5/007 , C12N9/88 , C12Y402/03047 , C12Y402/03073
摘要： The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
摘要翻译：本发明涉及倍半萜合酶及其生产和使用的方法。特别地，本发明提供了包含编码至少一个CVS的柑橘类戊烯合酶（CVS）的核苷酸序列的核酸。本发明还提供了包含编码形成CVS的1级和2级结构域的氨基酸残基的核苷酸序列的核酸。本发明还提供制备和使用本发明的核酸和氨基酸的方法。

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