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1. 发明授权

US5837494A E. coli mutant with suppressed organic acid production 失效
标题翻译：具有抑制有机酸生产的大肠杆菌突变体
公开(公告)号：US5837494A
公开(公告)日：1998-11-17
申请号：US860529
申请日：1997-06-30
申请人： Jong Ho Lee , Han Choi , Il Lae Jung , Doe Sun Na , Young Min Park
发明人： Jong Ho Lee , Han Choi , Il Lae Jung , Doe Sun Na , Young Min Park
IPC分类号： C12N15/00 , C07K14/47 , C12N1/20 , C12N1/21 , C12N15/12 , C12N15/70 , C12P21/02 , C12R1/19 , C12P21/06
CPC分类号： C07K14/4721 , C12N15/70 , C12R1/19 , Y10S435/849
摘要： Disclosed herein is a new E. coli mutant, which is not capable of growing under anaerobic cultivation conditions, said mutant being capable of utilizing glucose as a carbon source but having a suppressed organic acid production under aerobic cultivation conditions. The mutant can advantageously be employed as an expression host system to produce recombinant proteins.
摘要翻译： PCT No.PCT / KR96 / 00186 Sec。 371日期：1997年6月30日 102（e）日期1996年6月30日PCT提交1996年10月29日PCT公布。公开号WO97 / 16530 日期1997年5月9日这里公开的是一种新的大肠杆菌突变体，其不能在厌氧培养条件下生长，所述突变体能够利用葡萄糖作为碳源，但在需氧培养条件下具有抑制的有机酸产生。该突变体可有利地用作表达宿主系统以产生重组蛋白。

2. 发明申请

US20110111400A1 METHOD FOR ENHANCING CHEMICAL SENSITIVITY OR RADIOSENSITIVITY OF CANCER CELLS BY INHIBITING EXPRESSION OF TSPYL5 有权
标题翻译：通过抑制TSPYL5的表达来增强癌细胞的化学敏感性或放射敏感性的方法
公开(公告)号：US20110111400A1
公开(公告)日：2011-05-12
申请号：US12823035
申请日：2010-06-24
申请人： In Gyu KIM , Tae Lim Kim , Kug Chan Kim , Eun Jin Kim , So Yong Lee , Il Lae Jung
发明人： In Gyu KIM , Tae Lim Kim , Kug Chan Kim , Eun Jin Kim , So Yong Lee , Il Lae Jung
IPC分类号： C12Q1/68 , C12N5/02 , G01N33/53
CPC分类号： G01N33/5011 , G01N33/574
摘要： Disclosed herein is a method for enhancing sensitivity of cancer cells to compounds or radiation by inhibiting the expression of testis-specific protein, Y-encoded like 5 (TSPYL5). More specifically, because methylation of TSPYL5 protein expressed in lung cancer cell line was inhibited to increase the expression level of the gene, resistance to stress such as radiation or anticancer agents was increased. Because the sensitivity of cancer cells to stress such as radiation or anticancer agents was increased by inhibiting the expression of the TSPYL5 gene to promote the apoptosis of the cells, an anticancer supplement agent containing an inhibitor of the expression or activity of the TSPYL5 gene of the present invention inhibits the growth of cancer cells and enhances the sensitivity to various stresses to maximize the apoptosis. Thus, when used in combination with radiotherapy or chemotherapy, the anticancer supplement agent may be used very usefully for anticancer treatment.
摘要翻译：本文公开了通过抑制Y编码的5（TSPYL5）的睾丸特异性蛋白质的表达来增强癌细胞对化合物或辐射的敏感性的方法。更具体地说，由于在肺癌细胞系中表达的TSPYL5蛋白的甲基化被抑制以增加基因的表达水平，所以对抗应激性（如放射线或抗癌剂）的抗性增加。因为通过抑制TSPYL5基因的表达以促进细胞凋亡来增加癌细胞对诸如放射线或抗癌剂之类的应激的敏感性，所以可以使用含有TSPYL5基因的表达或活性抑制剂的抗癌补充剂本发明抑制癌细胞的生长并且增强对各种应激的敏感性以使细胞凋亡最大化。因此，当与放射治疗或化疗组合使用时，抗癌补充剂可以非常有用地用于抗癌治疗。

3. 发明授权

US08343940B2 Method for enhancing chemical sensitivity or radiosensitivity of cancer cells by inhibiting expression of TSPYL5 有权
标题翻译：通过抑制TSPYL5的表达来提高癌细胞的化学敏感性或放射敏感性的方法
公开(公告)号：US08343940B2
公开(公告)日：2013-01-01
申请号：US12823035
申请日：2010-06-24
申请人： In Gyu Kim , Tae Lim Kim , Kug Chan Kim , Eun Jin Kim , So Yong Lee , Il Lae Jung
发明人： In Gyu Kim , Tae Lim Kim , Kug Chan Kim , Eun Jin Kim , So Yong Lee , Il Lae Jung
IPC分类号： C12N15/11 , C07H21/02 , C07H21/04
CPC分类号： G01N33/5011 , G01N33/574
摘要： Disclosed herein is a method for enhancing sensitivity of cancer cells to compounds or radiation by inhibiting the expression of testis-specific protein, Y-encoded like 5 (TSPYL5). More specifically, because methylation of TSPYL5 protein expressed in lung cancer cell line was inhibited to increase the expression level of the gene, resistance to stress such as radiation or anticancer agents was increased. Because the sensitivity of cancer cells to stress such as radiation or anticancer agents was increased by inhibiting the expression of the TSPYL5 gene to promote the apoptosis of the cells, an anticancer supplement agent containing an inhibitor of the expression or activity of the TSPYL5 gene of the present invention inhibits the growth of cancer cells and enhances the sensitivity to various stresses to maximize the apoptosis. Thus, when used in combination with radiotherapy or chemotherapy, the anticancer supplement agent may be used very usefully for anticancer treatment.
摘要翻译：本文公开了通过抑制Y编码的5（TSPYL5）的睾丸特异性蛋白质的表达来增强癌细胞对化合物或辐射的敏感性的方法。更具体地说，由于在肺癌细胞系中表达的TSPYL5蛋白的甲基化被抑制以增加基因的表达水平，所以对抗应激性（如放射线或抗癌剂）的抗性增加。因为通过抑制TSPYL5基因的表达以促进细胞凋亡来增加癌细胞对诸如放射线或抗癌剂之类的应激的敏感性，所以可以使用含有TSPYL5基因的表达或活性抑制剂的抗癌补充剂本发明抑制癌细胞的生长并且增强对各种应激的敏感性以使细胞凋亡最大化。因此，当与放射治疗或化疗组合使用时，抗癌补充剂可以非常有用地用于抗癌治疗。

4. 发明授权

US07771964B2 Method of overproduction of polyhydroxybutyrate 有权
标题翻译：聚羟基丁酸酯过量生产的方法
公开(公告)号：US07771964B2
公开(公告)日：2010-08-10
申请号：US10998473
申请日：2004-11-29
申请人： In Gyu Kim , Il Lae Jung , Hyo Kook Park , Kug Chan Kim , Byung Ho Choi
发明人： In Gyu Kim , Il Lae Jung , Hyo Kook Park , Kug Chan Kim , Byung Ho Choi
IPC分类号： C12P1/00 , C12P7/00 , C12P7/02 , C12P7/18
CPC分类号： C12P7/62
摘要： The present invention relates to a recombinant Escherichia coli having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate using the same, more precisely, a recombinant Escherichia coli ‘MG1655/pTZ18U-PHB’ or ‘JIL938/pTZ18U-PHB’ having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate comprising the steps of preparing transformed Escherichia coli having phbCAB originated from Alcaligenes eutrophus, inoculating and culturing the cells (growth phase), inducing the production of polyhydroxyalkanoate in the recomibnant Escherichia coli (stationary phase and producing phase), and inducing the extracellular secretion of the polyhydroxyalkanoate from the recombinant Escherichia coli. The method of the present invention facilitates not only overproduction of polyhydroxyalkanoate such as polyhydroxybutyrate by a simple batch culture but also industrial use of biodegradable polyhydroxyalkanoate replacing conventional non-biodegradable plastics by its simple and easy fermentation, separation and purification process.
摘要翻译：本发明涉及具有源自嗜碱性杆菌的phbCAB的重组大肠杆菌，以及使用该重组大肠杆菌的具有phbCAB的重组大肠杆菌MG1655 / pTZ18U-PHB'或'JIL938 / pTZ18U-PHB'的多羟基链烷酸酯的过量产生方法来自产碱杆菌属的富含嗜碱性细菌的产生方法和多羟基链烷酸酯的过量产生方法，包括以下步骤：制备具有源自产碱杆菌属细菌的phbCAB的转化大肠杆菌，接种和培养细胞（生长期），诱导在大肠杆菌（稳定期和生产阶段）中产生聚羟基链烷酸酯），并诱导来自重组大肠杆菌的聚羟基链烷酸酯的细胞外分泌。本发明的方法不仅通过简单的分批培养而不仅过度生产聚羟基链烷酸酯如聚羟基丁酸酯，而且还通过其简单且容易的发酵，分离和纯化方法替代常规的不可生物降解的塑料来生产可降解的聚羟基链烷酸酯的工业应用。

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