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1. 发明申请

US20100300563A1 Modular device and method for moving fluids to and from a sample delivery element 审中-公开
标题翻译：用于将流体移动到和从样品传送元件移动的模块化装置和方法
公开(公告)号：US20100300563A1
公开(公告)日：2010-12-02
申请号：US12802063
申请日：2010-05-27
申请人： John Ramunas , Juan G. Santiago , Helen M. Blau , Keith T. Wong , Viktor Shkolnikov , Karl Stahl , Khaesha Hall , Kevin Nam Truong , Jason L. Chua
发明人： John Ramunas , Juan G. Santiago , Helen M. Blau , Keith T. Wong , Viktor Shkolnikov , Karl Stahl , Khaesha Hall , Kevin Nam Truong , Jason L. Chua
IPC分类号： F16K31/12 , F16K31/48
CPC分类号： G01N35/00 , Y10T137/85978 , Y10T137/86445
摘要： Provided is a sequenced fluid control device that includes a pneumatic driver module, a fluid cartridge having at least one fluid chamber and at least one waste chamber, and at least one sample delivery element disposed to sealably contact the fluid cartridge forming a main chamber containing a sample, where the fluid cartridge is also disposed to sealably contact the pneumatic driver module. The pneumatic driver module injects gas into or withdraws gas from the fluid cartridge, where the gas directly contacts at least one fluid causing at least one fluid to flow through channels disposed to connect at least one fluid chamber to the sample delivery element and disposed to connect the sample delivery element to the at least one waste chamber or waste channel, where a sample on the sample delivery element is exposed to the at least one fluid.
摘要翻译：提供了一种排序的流体控制装置，其包括气动驱动器模块，具有至少一个流体室和至少一个废物室的流体盒，以及设置成密封地接触形成包含样品，其中流体盒也设置成可密封地接触气动驱动器模块。气动驱动器模块将气体注入或从气体中抽出气体，其中气体直接接触至少一种流体，导致至少一种流体流过设置成将至少一个流体室连接到样品传送元件的通道并被设置成连接样品递送元件到达至少一个废物室或废物通道，其中样品递送元件上的样品暴露于至少一种流体。

2. 发明授权

US08382460B2 Peristaltic pump with constrictions at fixed locations 失效
标题翻译：蠕动泵在固定位置收缩
公开(公告)号：US08382460B2
公开(公告)日：2013-02-26
申请号：US12290650
申请日：2008-10-31
申请人： John Ramunas , Juan G. Santiago , David P Fenning , Viktor Shkolnikov
发明人： John Ramunas , Juan G. Santiago , David P Fenning , Viktor Shkolnikov
IPC分类号： F04B43/08
CPC分类号： F04B43/082 , F04B43/123
摘要： In a pump, a flexible tube passes through the pump and is held “normally closed” at an output constriction. The pump includes a pump body and a pump member that can perform two functions by their relative motion. The first function is to open and close an input constriction of the flexible tube. The second function is to compress the section of flexible tube between the input constriction and the output constriction. This section of tube acts as the pump chamber. When closed, the input constriction provides a greater impediment to fluid flow than the output constriction. Therefore, when the input constriction is closed and the pump chamber is compressed, fluid flows out of the pump past the output constriction. When the input constriction is open and compression of the pump chamber is removed, the output constriction closes and fluid can enter the pump by flowing past the location of the input constriction.
摘要翻译：在泵中，柔性管通过泵并且在输出收缩处保持常闭。泵包括泵体和泵构件，其可以通过它们的相对运动来执行两个功能。第一个功能是打开和关闭柔性管的输入收缩。第二个功能是在输入收缩和输出收缩之间压缩柔性管的部分。管部分作为泵室。当闭合时，输入收缩提供了比输出收缩更大的阻碍流体流动的障碍。因此，当输入收缩闭合并且泵室被压缩时，流体从泵流出超过输出收缩部。当输入收缩开启并且泵室的压缩被去除时，输出收缩闭合，并且流体可以通过流过输入收缩部的位置而进入泵。

3. 发明申请

US20100111733A1 Peristaltic pump with constrictions at fixed locations 失效
标题翻译：蠕动泵在固定位置收缩
公开(公告)号：US20100111733A1
公开(公告)日：2010-05-06
申请号：US12290650
申请日：2008-10-31
申请人： John Ramunas , Juan G. Santiago , David P. Fenning , Viktor Shkolnikov
发明人： John Ramunas , Juan G. Santiago , David P. Fenning , Viktor Shkolnikov
IPC分类号： F04B43/08
CPC分类号： F04B43/082 , F04B43/123
摘要： In a pump, a flexible tube passes through the pump and is held “normally closed” at an output constriction. The pump includes a pump body and a pump member that can perform two functions by their relative motion. The first function is to open and close an input constriction of the flexible tube. The second function is to compress the section of flexible tube between the input constriction and the output constriction. This section of tube acts as the pump chamber. When closed, the input constriction provides a greater impediment to fluid flow than the output constriction. Therefore, when the input constriction is closed and the pump chamber is compressed, fluid flows out of the pump past the output constriction. When the input constriction is open and compression of the pump chamber is removed, the output constriction closes and fluid can enter the pump by flowing past the location of the input constriction.
摘要翻译：在泵中，柔性管通过泵并且在输出收缩处保持“常闭”。泵包括泵体和泵构件，其可以通过它们的相对运动来执行两个功能。第一个功能是打开和关闭柔性管的输入收缩。第二个功能是在输入收缩和输出收缩之间压缩柔性管的部分。管部分作为泵室。当闭合时，输入收缩提供了比输出收缩更大的阻碍流体流动的障碍。因此，当输入收缩闭合并且泵室被压缩时，流体从泵流出超过输出收缩部。当输入收缩开启并且泵室的压缩被去除时，输出收缩闭合，并且流体可以通过流过输入收缩部的位置而进入泵。

4. 发明申请

US20070161106A1 Cultured cell and method and apparatus for cell culture 失效
标题翻译：培养细胞和细胞培养的方法和装置
公开(公告)号：US20070161106A1
公开(公告)日：2007-07-12
申请号：US10582975
申请日：2004-12-17
申请人： Eric Jervis , John Ramunas
发明人： Eric Jervis , John Ramunas
IPC分类号： C12N5/06
CPC分类号： C12M23/16 , B01L3/5027 , C12M25/06 , C12M29/04
摘要： One or more cells can be cultured when confined in space by barriers. The distance between barriers can be comparable to the size of a cell to be cultured. The space between barriers can also be sufficiently small to allow control of cell properties or monitoring of the cell(s) cultured therein. The cell(s) may be confined completely or may be mobile between two opposing barrier surfaces. The gap between two opposing barriers may be sufficiently narrow to allow only a monolayer of cells to be cultured. A barrier can be transparent. The surfaces of the barriers may have one or more pre-selected characteristics that mimic the characteristics of a biological niche of the cells(s). The number of cells in a cell culture may be limited to permit control of properties of individual cells. The cultured cell(s) may be monitored, such as imaged, over a long period of time, using standard bright field or fluorescent imaging techniques.
摘要翻译：当通过屏障限制在空间中时，可以培养一个或多个细胞。障碍物之间的距离可以与要培养的细胞的大小相当。障碍之间的空间也可以足够小以允许控制细胞特性或监测其中培养的细胞。细胞可以被完全限制或可以在两个相对的阻挡表面之间移动。两个相对的屏障之间的间隙可能足够窄以允许仅培养单层的细胞。屏障可以是透明的。障碍物的表面可以具有模拟细胞的生物学位置的特征的一个或多个预先选择的特征。可以限制细胞培养物中的细胞数量以允许控制各细胞的性质。使用标准亮场或荧光成像技术可以长时间监测培养的细胞，例如成像的。

5. 发明授权

US07919319B2 Cultured cell and method and apparatus for cell culture 失效
标题翻译：培养细胞和细胞培养的方法和装置
公开(公告)号：US07919319B2
公开(公告)日：2011-04-05
申请号：US10582975
申请日：2004-12-17
申请人： Eric Jervis , John Ramunas
发明人： Eric Jervis , John Ramunas
IPC分类号： C12N5/02
CPC分类号： C12M23/16 , B01L3/5027 , C12M25/06 , C12M29/04
摘要： One or more cells can be cultured when confined in space by barriers. The distance between barriers can be comparable to the size of a cell to be cultured. The space between barriers can also be sufficiently small to allow control of cell properties or monitoring of the cell(s) cultured therein. The cell(s) may be confined completely or may be mobile between two opposing barrier surfaces. The gap between two opposing barriers may be sufficiently narrow to allow only a monolayer of cells to be cultured. A barrier can be transparent. The surfaces of the barriers may have one or more pre-selected characteristics that mimic the characteristics of a biological niche of the cells(s). The number of cells in a cell culture may be limited to permit control of properties of individual cells. The cultured cell(s) may be monitored, such as imaged, over a long period of time, using standard bright field or fluorescent imaging techniques.
摘要翻译：当通过屏障限制在空间中时，可以培养一个或多个细胞。障碍物之间的距离可以与要培养的细胞的大小相当。障碍之间的空间也可以足够小以允许控制细胞特性或监测其中培养的细胞。细胞可以被完全限制或可以在两个相对的阻挡表面之间移动。两个相对的屏障之间的间隙可能足够窄以允许仅培养单层的细胞。屏障可以是透明的。障碍物的表面可以具有模拟细胞的生物学位置的特征的一个或多个预先选择的特征。可以限制细胞培养物中的细胞数量以允许控制各细胞的性质。使用标准亮场或荧光成像技术可以长时间监测培养的细胞，例如成像的。

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