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1. 发明授权

US06242259B1 Compositions and methods for packing of alphavirus vectors 有权
标题翻译：甲病毒载体的组合和方法
公开(公告)号：US06242259B1
公开(公告)日：2001-06-05
申请号：US09476299
申请日：1999-12-30
申请人： John Polo , Barbara Beli , Thomas W. Dubensky, Jr. , Stephen F. Hardy , Silvia Perri
发明人： John Polo , Barbara Beli , Thomas W. Dubensky, Jr. , Stephen F. Hardy , Silvia Perri
IPC分类号： C12N510
CPC分类号： C12N7/00 , A61K48/00 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/86 , C12N2770/36122 , C12N2770/36143 , C12N2770/36152 , C12N2830/00 , C12N2830/60
摘要： Nucleic acid molecule are provided comprising a nucleic acid sequence which encodes, in order, an alphavirus capsid, a signal peptide, and an alphavirus E1 or E2 glycoprotein. Also provided are vectors encoding such nucleic acid molecules, and use of such vectors or expression cassettes to generate recombinant alphavirus particles and alphavirus packaging cell lines. In addition, modified alphavirus vector constructs are provided that permit reduced transgene expression during vector packaging, as well as methods of using such vector constructs for the production of alphavirus vector particles.
摘要翻译：提供核酸分子，其包含按顺序编码甲病毒衣壳，信号肽和甲病毒属E1或E2糖蛋白的核酸序列。还提供了编码这种核酸分子的载体，以及使用这样的载体或表达盒产生重组甲病毒颗粒和甲病毒包装细胞系。此外，提供了修饰的甲病毒载体构建体，其允许在载体包装期间降低的转基因表达，以及使用这种载体构建体用于产生甲病毒载体颗粒的方法。

2. 发明授权

US06329201B1 Compositions and methods for packaging of alphavirus vectors 有权
标题翻译：包装甲病毒载体的组合物和方法
公开(公告)号：US06329201B1
公开(公告)日：2001-12-11
申请号：US09609154
申请日：2000-06-30
申请人： John M. Polo , Thomas W. Dubensky, Jr. , Stephen F. Hardy , Silvia Perri
发明人： John M. Polo , Thomas W. Dubensky, Jr. , Stephen F. Hardy , Silvia Perri
IPC分类号： C12N1586
CPC分类号： C07K14/005 , A61K48/00 , C07K2319/00 , C07K2319/02 , C12N7/00 , C12N15/86 , C12N2770/36122 , C12N2770/36143 , C12N2770/36152 , C12N2830/00 , C12N2830/60
摘要： Nucleic acid molecule are provided comprising a nucleic acid sequence which encodes, in order, an alphavirus capsid, a signal peptide, and an alphavirus E1 or E2 glycoprotein. Also provided are vectors encoding such nucleic acid molecules, and use of such vectors or expression cassettes to generate recombinant alphavirus particles and alphavirus packaging cell lines. In addition, modified alphavirus vector constructs are provided that permit reduced transgene expression during vector packaging, as well as methods of using such vector constructs for the production of alphavirus vector particles.
摘要翻译：提供核酸分子，其包含按顺序编码甲病毒衣壳，信号肽和甲病毒属E1或E2糖蛋白的核酸序列。还提供了编码这种核酸分子的载体，以及使用这样的载体或表达盒产生重组甲病毒颗粒和甲病毒包装细胞系。此外，提供了修饰的甲病毒载体构建体，其允许在载体包装期间减少的转基因表达，以及使用这种载体构建体用于产生甲病毒载体颗粒的方法。

3. 发明申请

US20100086565A1 Vectors for expression of hml-2 polypeptides 失效
标题翻译：用于表达hml-2多肽的载体
公开(公告)号：US20100086565A1
公开(公告)日：2010-04-08
申请号：US10587032
申请日：2003-06-13
申请人： Stephen F. Hardy , John Donnelly , Jan zur Megede
发明人： Stephen F. Hardy , John Donnelly , Jan zur Megede
IPC分类号： A61K39/12 , C12N15/74 , A61K31/7088 , C12N7/00 , C12Q1/70 , G01N33/53 , A61P37/04 , A61P35/00
CPC分类号： A61K39/21 , A61K39/12 , A61K2039/5258 , A61K2039/53 , A61K2039/54 , A61K2039/545 , C07K14/005 , C12N2740/10022 , C12N2740/10034
摘要： A nucleic acid vector comprising: (i) a promoter; (ii) a sequence encoding a HML-2 polypeptide operably linked to said promoter; and (iii) a selectable marker. Preferred vectors comprise: (I) a eukaryotic promoter; (ii) a sequence encoding a HML-2 polypeptide downstream of and operably linked to said promoter, (iii) a prokaryotic selectable marker; (iv) a prokaryotic origin of replication; and (v) a eukaryotic transcription terminator downstream of and operably linked to said sequence encoding a HML-2 polypeptide. Vectors of the invention are particularly useful for expression of HML-2 polypeptides either in vitro (e.g. for later purification). Or in vivo (e.g. for nucleic acid immunization). They are well suited to nucleic acid immunization against prostrate tumors. A preferred HML-2 is PCAV, which is located in chromosome 22 at 20.428 megabases (22q11.2).
摘要翻译：一种核酸载体，其包含：（i）启动子; （ii）编码与所述启动子可操作地连接的HML-2多肽的序列; 和（iii）可选标记。优选的载体包括：（I）真核启动子; （ii）编码在所述启动子下游并可操作地连接的HML-2多肽的序列，（iii）原核选择标记; （iv）原核复制起点; 和（v）在编码HML-2多肽的所述序列的下游并可操作地连接真核转录终止子。本发明的载体在体外（例如用于后续纯化）中特别可用于表达HML-2多肽。或体内（例如用于核酸免疫）。它们非常适合于针对前列腺肿瘤的核酸免疫。优选的HML-2是PCAV，其位于染色体22上，为20.428兆比特（22q11.2）。

4. 发明授权

US06228646B1 Helper-free, totally defective adenovirus for gene therapy 失效
标题翻译：无辅助，完全缺陷的腺病毒用于基因治疗
公开(公告)号：US06228646B1
公开(公告)日：2001-05-08
申请号：US08812618
申请日：1997-03-07
申请人： Stephen F. Hardy
发明人： Stephen F. Hardy
IPC分类号： C12N1500
CPC分类号： C12N15/86 , C12N7/00 , C12N2710/10343 , C12N2710/10362 , C12N2800/30 , C12N2830/38
摘要： A method for producing in vivo packaged recombinant adenovirus vectors is provided. The recombinant Ad vectors do not contain any Adenovirus genes and are therefore useful for gene therapy. The recombinant Adenovirus vectors are packaged in vivo using a helper virus which is itself very inefficiently packaged, providing a recombinant viral preparation with very little or no contamination with helper virus. In particular, the method makes use of a helper virus in which the packaging site can be easily excised in vivo by recombination mediated by a recombinase. The helper virus is also useful for the in vivo construction of new recombinant adenovirus vectors containing substitutions in the E1 or other adenoviral region.
摘要翻译：提供了一种生产体内包装的重组腺病毒载体的方法。重组Ad载体不含有任何腺病毒基因，因此可用于基因治疗。重组腺病毒载体使用本身非常低效包装的辅助病毒在体内包装，提供与辅助病毒很少或没有污染的重组病毒制剂。特别地，该方法利用辅助病毒，其中可以通过由重组酶介导的重组在体内容易地切除包装位点。辅助病毒也可用于体内构建在E1或其他腺病毒区域中含有取代的新的重组腺病毒载体。

5. 发明申请

US20090214506A1 Use of TFPI to Treat Severe Bacterial Infections 审中-公开
标题翻译：使用TFPI治疗严重的细菌感染
公开(公告)号：US20090214506A1
公开(公告)日：2009-08-27
申请号：US11918662
申请日：2006-05-08
申请人： Stephen F. Hardy , Yumin Dai
发明人： Stephen F. Hardy , Yumin Dai
IPC分类号： A61K38/44 , A61K38/16
CPC分类号： A61K38/57
摘要： Methods for prophylactically or therapeutically treating a patient at risk of developing or diagnosed as having a severe bacterial infection involving administration of tissue factor pathway inhibitor (TFPI) or a TFPI analog to patients suffering from or at risk of developing this condition. The methods involve the use of continuous intravenous infusion of TFPI or a TFPI analog at low doses to avoid adverse side effects.
摘要翻译：用于预防或治疗患有发展或诊断为具有严重细菌感染的患者的患者的方法，所述严重细菌感染涉及组织因子途径抑制剂（TFPI）或TFPI类似物给患有或有发展该病症风险的患者。该方法包括以低剂量连续静脉输注TFPI或TFPI类似物以避免不良副作用。

6. 发明授权

US06429001B1 Recombinant AAV packaging systems 失效
公开(公告)号：US06429001B1
公开(公告)日：2002-08-06
申请号：US09770315
申请日：2001-01-26
申请人： Stephen F. Hardy
发明人： Stephen F. Hardy
IPC分类号： C12N700
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2740/13043 , C12N2750/14143 , C12N2750/14152 , C12N2800/30
摘要： Methods and compositions are provided for producing recombinant AAV vector particles; comprising the general steps of (a) introducing into a host cell (i) pfloxAAV, (ii) a recombinant viral vector encoding plasmid, and (iii) a plasmid encoding herpesvirus, cytomegalovirus, or adenoviral functions, or a herpesvirus, cytomegalovirus, or, adenovirus itself, in order to produce flox AAV particles and recombinant AAV particles; and (b) introducing into a second host cell (i) the recombinant AAV particles and flox AAV particles of (a), (ii) a vector which directs the expression of Cre, and (iii) a vector which directs the expression of herpesvirus, CMV, or adenovirus helper functions, such that said recombinant AAV vector particles are produced.

7. 发明授权

US07776523B2 Endogenous retroviruses up-regulated in prostate cancer 有权
标题翻译：内源性逆转录病毒在前列腺癌中上调
公开(公告)号：US07776523B2
公开(公告)日：2010-08-17
申请号：US10016604
申请日：2001-12-07
申请人： Pablo D. Garcia , Stephen F. Hardy , Jaime Escobedo , Lewis T. Williams
发明人： Pablo D. Garcia , Stephen F. Hardy , Jaime Escobedo , Lewis T. Williams
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/505 , A61K2039/53 , C12N7/00 , C12N2740/10021 , C12N2740/10022 , C12Q1/6886 , C12Q1/702 , C12Q2600/158 , G01N33/57434 , G01N2333/15
摘要： Human endogenous retroviruses of the HML-2 family show up-regulated expression in prostate tumors. This finding can be used in prostate cancer screening, diagnosis and therapy.
摘要翻译： HML-2家族的人内源性逆转录病毒在前列腺肿瘤中显示出上调的表达。这一发现可用于前列腺癌筛查，诊断和治疗。

8. 发明申请

US20100136522A1 ENDOGENOUS RETROVIRUSES UP-REGULATED IN PROSTATE CANCER 有权
标题翻译：在前列腺癌中上调的内源性逆转录酶
公开(公告)号：US20100136522A1
公开(公告)日：2010-06-03
申请号：US10016604
申请日：2001-12-07
申请人： Pablo D. Garcia , Stephen F. Hardy , Jaime Escobedo , Lewis T. Williams
发明人： Pablo D. Garcia , Stephen F. Hardy , Jaime Escobedo , Lewis T. Williams
IPC分类号： C12Q1/68
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/505 , A61K2039/53 , C12N7/00 , C12N2740/10021 , C12N2740/10022 , C12Q1/6886 , C12Q1/702 , C12Q2600/158 , G01N33/57434 , G01N2333/15
摘要： Human endogenous retroviruses of the HML-2 family show up-regulated expression in prostate tumors. This finding can be used in prostate cancer screening, diagnosis and therapy.
摘要翻译： HML-2家族的人内源性逆转录病毒在前列腺肿瘤中显示出上调的表达。这一发现可用于前列腺癌筛查，诊断和治疗。

9. 发明授权

US06586208B2 Use of suppressor tRNA's to regulate cytotoxicity during the production of recombinant gene products 失效
公开(公告)号：US06586208B2
公开(公告)日：2003-07-01
申请号：US09784130
申请日：2001-02-16
申请人： Brian A. Donahue , Stephen F. Hardy , Richard O. Snyder
发明人： Brian A. Donahue , Stephen F. Hardy , Richard O. Snyder
IPC分类号： C12N701
CPC分类号： C12N7/00 , C12N15/63 , C12N15/86 , C12N2710/10343 , C12N2710/10344 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2840/002
摘要： Suppressor tRNA's are used to regulate expression of transgenes that are toxic, or the expression thereof requires a factor that is toxic, to the host cell.

10. 发明授权

US06423544B1 Compositions and methods for producing recombinant virions 失效
标题翻译：用于产生重组病毒粒子的组合物和方法
公开(公告)号：US06423544B1
公开(公告)日：2002-07-23
申请号：US09608730
申请日：2000-06-30
申请人： Stephen F. Hardy
发明人： Stephen F. Hardy
IPC分类号： C12N1586
CPC分类号： C07K14/005 , A61K48/00 , C07K2319/00 , C07K2319/02 , C12N7/00 , C12N9/00 , C12N15/63 , C12N15/86 , C12N2750/14152 , C12N2770/36122 , C12N2770/36152 , C12N2830/00 , C12N2830/60
摘要： The invention provides host cells comprising a translation operator sequence (TOP) and packaging elements. Also provided are viral vectors comprising a TOP operably linked to a transgene. Also provided are methods of using these host cells and viral vectors to produce recombinant virions.
摘要翻译：本发明提供包含翻译操作员序列（TOP）和包装元件的宿主细胞。还提供了包含可操作地连接到转基因的TOP的病毒载体。还提供了使用这些宿主细胞和病毒载体产生重组病毒体的方法。

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