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    • 4. 发明申请
    • Grain wet milling process for producing ethanol
    • 谷物湿磨生产乙醇的方法
    • US20060251762A1
    • 2006-11-09
    • US11121295
    • 2005-05-03
    • Robert JansenJohn KerrEdward FarleyGordon WalkerSebastien Camborieux
    • Robert JansenJohn KerrEdward FarleyGordon WalkerSebastien Camborieux
    • A23L1/10
    • C12P7/06Y02E50/16Y02E50/17
    • Whole grain, such as wheat, barley, rye, and/or rice, can be processed by (a) steeping the grain in an aqueous liquid to produce softened grain, (b) milling the softened grain to produce milled grain, (c) liquefying the milled grain by contacting it with amylase and heating it to a temperature of at least about 50° C., producing a liquefied material, (d) at least partially saccharifying the liquefied material by contacting it with amyloglucosidase at a temperature of at least about 50° C., producing a first saccharified material, and (e) separating fiber and germ from the first saccharified material, producing a screened material that is substantially free of fiber and germ. The process also includes the steps of (f) further saccharifying and fermenting the screened material with a microorganism that produces ethanol, thereby producing a broth that comprises ethanol, soluble protein, and insoluble protein, and (g) separating ethanol from the broth. A protein-rich product can be recovered from the broth that comprises both gluten from the grain and microorganism from the fermenting step.
    • (a)在水溶液中浸渍谷物以产生软化谷粒,(b)研磨软化谷物以产生碾磨的谷物,(c)可以加工全谷物如小麦,大麦,黑麦和/或米,(c) 通过使其与淀粉酶接触并将其加热到至少约50℃的温度来液化碾磨的颗粒,产生液化材料,(d)至少部分地使液化物料与淀粉葡糖苷酶接触至少部分糖化, 约50℃,产生第一糖化物质,和(e)从第一糖化物质分离纤维和胚芽,产生基本上不含纤维和细菌的筛选材料。 该方法还包括以下步骤:(f)用生产乙醇的微生物进一步对筛选的材料进行糖化和发酵,从而产生包含乙醇,可溶性蛋白质和不溶性蛋白质的肉汤,和(g)从肉汤中分离乙醇。 富含蛋白质的产物可以从含有谷物的谷蛋白和发酵步骤的微生物的肉汤中回收。
    • 5. 发明申请
    • Grain wet milling process for producing dextrose
    • 用于生产葡萄糖的谷物湿磨工艺
    • US20060251761A1
    • 2006-11-09
    • US11121170
    • 2005-05-03
    • Robert JansenJohn KerrEdward FarleyGordon WalkerSebastien Camborieux
    • Robert JansenJohn KerrEdward FarleyGordon WalkerSebastien Camborieux
    • A23L1/10
    • C12Y302/00A23J1/125A23J3/18A23K10/30A23K20/179A23K50/80A23L7/104A23L29/35C13B20/142C13K1/08Y02A40/818
    • Whole grain, such as wheat, barley, rye, and/or rice, can be processed by (a) steeping the grain or at least partially dehulled grain in an aqueous liquid to produce softened grain, (b) milling the softened grain to produce milled grain, (c) liquefying the milled grain by contacting it with amylase and heating it to a temperature of at least about 50° C., producing a liquefied material, (d) at least partially saccharifying the liquefied material by contacting it with amyloglucosidase at a temperature of at least about 50° C., producing a first saccharified material, and (e) separating fiber and germ from the first saccharified material, producing a screened material that is substantially free of fiber and wheat germ. The process also includes the steps of (f) further saccharifying the screened material by contacting it with amyloglucosidase at a temperature of at least about 50° C., producing a second saccharified material, (g) membrane filtering the second saccharified material, producing a permeate that comprises primarily dextrose and other soluble components and a retentate that comprises insoluble protein, and (h) purifying the permeate by chromatographic separation, producing a purified dextrose stream. The chromatographic separation can also produce a raffinate, and the process can further include the steps of (i) combining the retentate from the membrane filtration and the raffinate from the chromatographic separation to form a fermentation medium, (j) fermenting the fermentation medium aerobically with a microorganism, (k) separating a protein product that comprises insoluble protein and microorganism from the medium, and (l) drying the protein product.
    • 可以通过(a)在水性液体中浸渍颗粒或至少部分去壳的颗粒以产生软化的颗粒,(b)研磨软化的颗粒以产生(例如,麦粒,大麦,黑麦和/或米) (c)通过使其与淀粉酶接触并将其加热到至少约50℃的温度来液化所研磨的颗粒,产生液化材料,(d)通过使液化物质与淀粉葡糖苷酶接触而至少部分地糖化 在至少约50℃的温度下,产生第一糖化材料,和(e)从第一糖化物质分离纤维和胚芽,产生基本上不含纤维和小麦胚芽的筛选材料。 该方法还包括以下步骤:(f)通过使筛选的材料与淀粉葡糖苷酶在至少约50℃的温度下接触进一步糖化,产生第二糖化物质,(g)膜过滤第二糖化物质,产生 主要包含葡萄糖和其他可溶性组分的渗透物和包含不溶性蛋白质的渗余物,和(h)通过色谱分离纯化渗透物,产生纯化的葡萄糖流。 色谱分离还可以产生萃余液,并且该方法还可以包括以下步骤:(i)将来自膜过滤的残留物和来自色谱分离的萃余液组合以形成发酵培养基,(j)有氧地发酵发酵培养基, 微生物,(k)从培养基中分离含有不溶性蛋白质和微生物的蛋白质产物,和(l)干燥蛋白质产物。
    • 6. 发明申请
    • Sucralose production method
    • 三氯蔗糖生产方法
    • US20080234472A1
    • 2008-09-25
    • US12009455
    • 2008-01-18
    • Duane LeinhosJohn KerrBob JansenSebastien CamborieuxPeter Lloyd-JonesAnthony Baiada
    • Duane LeinhosJohn KerrBob JansenSebastien CamborieuxPeter Lloyd-JonesAnthony Baiada
    • C07H1/00
    • C07H5/02
    • There is provided a method for the production of sucralose from a feed stream resulting from the chlorination of a sucrose-6-acylate in a reaction vehicle, said feed stream comprising a sucralose-6-acylate, the reaction vehicle, and by-products including high molecular weight coloured material, said method comprising: deacylation of the sucralose-6-acylate by treatment with a base to afford sucralose, and, before or after said deacylation, removal of the reaction vehicle and isolation of the sucralose characterised in that immediately before the removal of the reaction vehicle, the reaction stream is subjected to a precipitation step comprising treatment with a metal or ammonium hydroxide and carbon dioxide to form a precipitate of the corresponding metal or ammonium carbonate in which at least a portion of said high molecular weight coloured material is trapped, followed by separation of said precipitate.
    • 提供了从反应载体中的蔗糖-6-酰化物氯化得到的进料流中生产三氯蔗糖的方法,所述进料流包含三氯半乳蔗糖-6-酰化物,反应载体和副产物,包括 所述方法包括:用碱处理三氯半乳蔗糖-6-酰化物脱酰基,得到三氯半乳蔗糖,并且在所述脱酰化之前或之后,除去反应载体和分离三氯半乳蔗糖,其特征在于,紧接在之前 去除反应载体,将反应物流进行沉淀步骤,包括用金属或氢氧化铵和二氧化碳处理以形成相应的金属或碳酸铵的沉淀物,其中至少部分所述高分子量着色 材料被捕获,然后分离所述沉淀物。