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    • 5. 发明申请
    • Dot-Tag Visibility Network Architecture
    • 点标签可见性网络架构
    • US20100322168A1
    • 2010-12-23
    • US12705115
    • 2010-02-12
    • John K. StevensDavid CramerTom Packert
    • John K. StevensDavid CramerTom Packert
    • H04W4/00
    • G06Q10/087
    • The system employs full transceivers, each having peer-to-peer, client/server, and IP networking capabilities, and covering open-area ranges of up to 100 feet. The system uses Low Frequency for data communications so it can achieve both low cost (less costly than many RF-ID tags) and long battery life (10-15 years). Additionally, since these tags have batteries, static RAM maybe be added at very low cost, as well as sensors, LED's displays etc. The system also employs a sidewinder that communicates to said tag regularly, said sidewinder keeps the IP address of said tag. The system also employs an embedded VPN. The VPN includes a suite of diagnostic tools that run on a laptop. The diagnostic tools talk direct to the sidewinder, the tools read and program said tag providing the tools have correct IP address of the tag. The system also includes a visibility data server that communicates to the sidewinder by the VPN. The data server includes a virtual tag data base, said virtual tag data base updated by the sidewinder. The web enabled reports and control are managed by two “plugs”. The SQL plug supports data requests, and the Air Traffic Control (ATC) plug provides control (LED's, read rates). The system also employs a user web based ERP to create a real-time visibility reports and a user event export tools to create reports that fit a specific event.
    • 该系统采用完整的收发器,每个收发器具有点对点,客户端/服务器和IP网络功能,并覆盖高达100英尺的开放范围。 该系统使用低频进行数据通信,因此可以实现低成本(比许多RF-ID标签更便宜)和更长的电池寿命(10-15年)。 此外,由于这些标签具有电池,因此可以以非常低的成本添加静态RAM,以及传感器,LED显示器等。该系统还采用定期与所述标签通信的侧卷器,所述侧卷纸保持所述标签的IP地址。 该系统还采用嵌入式VPN。 VPN包括一套在膝上型计算机上运行的诊断工具。 诊断工具直接与副卷轴通信,读取和编程所述标签的工具提供工具具有正确的标签IP地址。 该系统还包括一个可视数据服务器,它通过VPN与主持人进行通信。 数据服务器包括虚拟标签数据库,所述虚拟标签数据库由所述侧挂方更新。 网络启用报告和控制由两个“插头”管理。 SQL插件支持数据请求,空中交通管制(ATC)插头提供控制(LED,读取速率)。 该系统还使用基于用户网络的ERP来创建实时可见性报告和用户事件导出工具来创建适合特定事件的报告。
    • 6. 发明授权
    • Dot-tag visibility network architecture
    • 点标签可见性网络架构
    • US08115628B2
    • 2012-02-14
    • US12705115
    • 2010-02-12
    • John K. StevensDavid CramerTom Packert
    • John K. StevensDavid CramerTom Packert
    • G08B13/14G06Q10/00G06F7/00
    • G06Q10/087
    • The system employs full transceivers, each having peer-to-peer, client/server, and IP networking capabilities, and covering open-area ranges of up to 100 feet. The system uses Low Frequency for data communications so it can achieve both low cost (less costly than many RF-ID tags) and long battery life (10-15 years). Additionally, since these tags have batteries, static RAM maybe be added at very low cost, as well as sensors, LED's displays etc. The system also employs a sidewinder that communicates to said tag regularly, said sidewinder keeps the IP address of said tag. The system also employs an embedded VPN. The VPN includes a suite of diagnostic tools that run on a laptop. The system also includes a visibility data server that communicates to the sidewinder by the VPN. The data server includes a virtual tag data base, said virtual tag data base updated by the sidewinder.
    • 该系统采用完整的收发器,每个收发器具有点对点,客户端/服务器和IP网络功能,并覆盖高达100英尺的开放范围。 该系统使用低频进行数据通信,因此可以实现低成本(比许多RF-ID标签更便宜)和更长的电池寿命(10-15年)。 此外,由于这些标签具有电池,因此可以以非常低的成本添加静态RAM,以及传感器,LED显示器等。该系统还采用定期与所述标签通信的侧卷器,所述侧卷纸保持所述标签的IP地址。 该系统还采用嵌入式VPN。 VPN包括一套在膝上型计算机上运行的诊断工具。 该系统还包括一个可视数据服务器,它通过VPN与主持人进行通信。 数据服务器包括虚拟标签数据库,所述虚拟标签数据库由所述侧挂方更新。
    • 9. 发明授权
    • Method and system for DNA sequence determination and mutation detection
    • 用于DNA序列测定和突变检测的方法和系统
    • US06303303B1
    • 2001-10-16
    • US09190756
    • 1998-11-12
    • Ronald J. GreenVrijmoed ChiRodney D. GilchristGregory DeeJohn K. Stevens
    • Ronald J. GreenVrijmoed ChiRodney D. GilchristGregory DeeJohn K. Stevens
    • C12Q168
    • G01N27/44721G01N27/44717G06F19/18G06F19/22Y10T436/143333
    • Normalization of experimental fragment patterns for nucleic acid polymers having putatively known sequences starts with obtaining at least one raw fragment pattern for the experimental sample. The raw fragment pattern represents the positions of a selected nucleic acid base within the polymer as a function of migration time or distance. This raw fragment pattern is conditioned using conventional baseline correction and noise reduction technique to yield a clean fragment pattern. The clean fragment pattern is then evaluated to determine one or more “normalization coefficients.” These normalization coefficients reflect the displacement, stretching or shrinking, and rate of stretching or shrinking of the clean fragment, or segments thereof, which are necessary to obtain a suitably high degree of correlation between the clean fragment pattern and a standard fragment pattern which represents the positions of the selected nucleic acid base within a standard polymer actually having the known sequence as a function of migration time or distance. The normalization coefficients are then applied to the clean fragment pattern to produce a normalized fragment pattern which is used for base-calling in a conventional manner. This method may be implemented in an apparatus comprising a computer processor programmed to determine normalization coefficients for an experimental fragment pattern. This computer may be separate from the electrophoresis apparatus, or part of an integrated unit.
    • 具有推定已知序列的核酸聚合物的实验片段模式的归一化从获得实验样品的至少一个原始片段图案开始。 原始片段图案表示聚合物内所选择的核酸碱基作为迁移时间或距离的函数的位置。 使用常规的基线校正和降噪技术调节该原始片段模式以产生干净的片段模式。 然后评估干净的片段模式以确定一个或多个“归一化系数”。 这些归一化系数反映了清洁片段或其片段的位移,拉伸或缩小以及拉伸或收缩速率,这些片段或片段是清洁片段图案与标示片段图案之间适当高程度的相关性所必需的, 实际上具有已知序列的标准聚合物内所选核酸碱基的位置作为迁移时间或距离的函数。 然后将归一化系数应用于干净的片段模式以产生用于以常规方式的基本呼叫的归一化的片段模式。 该方法可以在包括被编程为确定实验片段模式的归一化系数的计算机处理器的装置中实现。 该计算机可以与电泳装置或集成单元的一部分分开。
    • 10. 发明授权
    • Method and system for DNA sequence determination and mutation detection
with reference to a standard
    • 参考标准的DNA序列测定和突变检测的方法和系统
    • US5853979A
    • 1998-12-29
    • US497202
    • 1995-06-30
    • Ronald J. GreenVrijmoed ChiRodney D. GilchristGregory DeeJohn K. Stevens
    • Ronald J. GreenVrijmoed ChiRodney D. GilchristGregory DeeJohn K. Stevens
    • C12Q1/68G01N27/447C12Q1/70G01N33/48G06K9/00
    • G01N27/44721G01N27/44717G06F19/18G06F19/22Y10T436/143333
    • Normalization of experimental fragment patterns for nucleic acid polymers having putatively known sequences starts with obtaining at least one raw fragment pattern for the experimental sample. The raw fragment pattern represents the positions of a selected nucleic acid base within the polymer as a function of migration time or distance. This raw fragment pattern is conditioned using conventional baseline correction and noise reduction technique to yield a clean fragment pattern. The clean fragment pattern is then evaluated to determine one or more "normalization coefficients." These normalization coefficients reflect the displacement, stretching or shrinking, and rate of stretching or shrinking of the clean fragment, or segments thereof, which are necessary to obtain a suitably high degree of correlation between the clean fragment pattern and a standard fragment pattern which represents the positions of the selected nucleic acid base within a standard polymer actually having the known sequence as a function of migration time or distance. The normalization coefficients are then applied to the clean fragment pattern to produce a normalized fragment pattern which is used for base-calling in a conventional manner. This method may be implemented in an apparatus comprising a computer processor programmed to determine normalization coefficients for an experimental fragment pattern. This computer may be separate from the electrophoresis apparatus, or part of an integrated unit.
    • 具有推定已知序列的核酸聚合物的实验片段模式的归一化从获得实验样品的至少一个原始片段图案开始。 原始片段图案表示聚合物内所选择的核酸碱基作为迁移时间或距离的函数的位置。 使用常规的基线校正和降噪技术调节该原始片段模式以产生干净的片段模式。 然后评估干净的片段模式以确定一个或多个“归一化系数”。 这些归一化系数反映了清洁片段或其片段的位移,拉伸或缩小以及拉伸或收缩速率,这些片段或片段是清洁片段图案与标示片段图案之间适当高程度的相关性所必需的, 实际上具有已知序列的标准聚合物内所选核酸碱基的位置作为迁移时间或距离的函数。 然后将归一化系数应用于干净的片段模式以产生用于以常规方式的基本呼叫的归一化的片段模式。 该方法可以在包括被编程为确定实验片段模式的归一化系数的计算机处理器的装置中实现。 该计算机可以与电泳装置或集成单元的一部分分开。