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1. 发明授权

US5288613A Enzyme-based biosensor system for monitoring the freshness of fish 失效
标题翻译：基于酶的生物传感器系统，用于监测鱼的新鲜度
公开(公告)号：US5288613A
公开(公告)日：1994-02-22
申请号：US802698
申请日：1991-12-05
申请人： John H. T. Luong , Keith B. Male , An L. Nguyen
发明人： John H. T. Luong , Keith B. Male , An L. Nguyen
IPC分类号： C12Q1/00 , C12Q1/26 , C12M1/40 , C12Q1/64 , G01N27/26
CPC分类号： C12Q1/005 , Y10S435/817
摘要： The present invention relates to a method for determining the degree of freshness of raw, frozen and processed edible fish by monitoring the degradation of adenine triphosphate to inosine monophosphate, inosine and hypoxanthine. This method comprises simultaneously determining, by use of a suitable amperometric electrode such as platinum vs. silver/silver chloride polarized at 0.7 V, the amount of uric acid and hydrogen peroxide resulting from the degradation of hypoxanthine by xanthine oxidase, the degradation of inosine by the combined action of nucleoside phosphorylase and xanthine oxidase and the degradation of inosine monophosphate by the combined action of nucleotidase, nucleoside phosphorylase and xanthine oxidase. Also within the scope of this invention is a method for the immobilization of nucleotidase on the walls of a polymeric tube such as polystyrene tube and the co-immobilization of nucleoside phosphorylase and xanthine oxidase on a porous polymeric membrane such as a nylon membrane.
摘要翻译：本发明涉及通过监测三磷酸腺苷对肌苷酸，肌苷和次黄嘌呤的降解来确定生的，冷冻和加工的食用鱼的新鲜度的方法。该方法包括通过使用合适的安培数电极，例如铂与银/氯化镍在0.7V极化的同时测定由黄嘌呤氧化酶降解次黄嘌呤而产生的尿酸和过氧化氢的量，肌苷的降解由核苷磷酸化酶和黄嘌呤氧化酶的联合作用以及通过核苷酸酶，核苷磷酸化酶和黄嘌呤氧化酶的联合作用降低单磷酸肌苷。在本发明的范围内也是一种将核苷酸酶固定在诸如聚苯乙烯管的聚合物管的壁上并将核苷磷酸化酶和黄嘌呤氧化酶共同固定在多孔聚合物膜如尼龙膜上的方法。

2. 发明授权

US5411866A Method and system for determining bioactive substances 失效
标题翻译：用于测定生物活性物质的方法和系统
公开(公告)号：US5411866A
公开(公告)日：1995-05-02
申请号：US39998
申请日：1993-03-30
申请人： John H. T. Luong , Keith B. Male , Maurice V. Cattaneo
发明人： John H. T. Luong , Keith B. Male , Maurice V. Cattaneo
IPC分类号： C12M1/40 , C12Q1/00 , C12Q1/26 , C12Q1/34 , G01N33/68 , C12Q1/54
CPC分类号： C12Q1/34 , C12M21/18 , C12M41/32 , C12Q1/005 , C12Q1/26 , G01N33/6812
摘要： An apparatus and method for measuring glutamine and glucose in certain cell cultures and body fluids in the presence of interfering endogenous compounds e.g. ascorbic acid, uric acid or glutamic acid. Prior to enzymatic degradation and amperometric detection of the products or elements resulting from the degradation, the interfering substances are retained by an anion exchanger disposed upstream of the enzymatic degradation site. The conditions of the method are controlled to utilize the difference of isoelectric points of the measured biosubstance and of the interfering compounds respectively.
摘要翻译：一种在干扰性内源性化合物的存在下测定某些细胞培养物和体液中的谷氨酰胺和葡萄糖的装置和方法，抗坏血酸，尿酸或谷氨酸。在由降解产生的产物或元件的酶降解和电流检测之前，干扰物质被设置在酶降解位点上游的阴离子交换剂保留。控制该方法的条件以分别利用所测量的生物物质和干扰化合物的等电点的差异。

3. 发明授权

US08900706B2 Cellulose nanocrystals from renewable biomass 有权
标题翻译：来自可再生生物质的纤维素纳米晶体
公开(公告)号：US08900706B2
公开(公告)日：2014-12-02
申请号：US13513334
申请日：2010-03-15
申请人： Chi Woon Leung , John H. T. Luong , Sabahudin Hrapovic , Edmond Lam , Yali Liu , Keith B. Male , Khaled Mahmoud , Denis Rho
发明人： Chi Woon Leung , John H. T. Luong , Sabahudin Hrapovic , Edmond Lam , Yali Liu , Keith B. Male , Khaled Mahmoud , Denis Rho
IPC分类号： B32B5/16
CPC分类号： C08B15/08 , C08B15/04 , Y10T428/2982
摘要： A process for producing cellulose nanocrystals (CNCs) involves providing a cellulosic material, contacting the cellulosic material with an inorganic persulfate at an elevated temperature to produce CNCs, and recovering the CNCs. The process permits one-step production of CNCs from vegetative biomasses such as flax and hemp. Cellulose nanocrystals produced by the process with carboxylic groups are more uniform and have higher aspect ratios than CNCs produced by prior art processes.
摘要翻译：生产纤维素纳米晶体（CNCs）的方法涉及提供纤维素材料，在高温下使纤维素材料与无机过硫酸盐接触以产生CNCs，并回收CNCs。该过程允许从植物生物量如亚麻和大麻一步生产CNCs。通过具有羧基的方法制备的纤维素纳米晶体比通过现有技术方法产生的CNCs更均匀并且具有更高的纵横比。

4. 发明授权

US5432274A Redox dye and method of preparation thereof using 2-hydroxypropyl-.beta.-cyclodextrin and 1,1'-dimethylferrocene 失效
标题翻译：氧化还原染料及其制备方法，使用2-羟丙基-β-环糊精和1,1'-二甲基二茂铁
公开(公告)号：US5432274A
公开(公告)日：1995-07-11
申请号：US97901
申请日：1993-07-28
申请人： John H. T. Luong , Keith B. Male , Shishan Zhao , R. Stephen Brown
发明人： John H. T. Luong , Keith B. Male , Shishan Zhao , R. Stephen Brown
IPC分类号： C08B37/00 , C09B57/10 , C12P5/00 , C12Q1/26 , C08B37/16 , C07F17/02 , C12N11/14 , C12P19/04
CPC分类号： C12Q1/26 , C08B37/0015 , C09B57/10
摘要： Ferricinium or ferricinium-based compounds are useful as blue redox dyes suitable for monitoring various enzymatic oxidation processes. A process for preparing such dyes is proposed which involves complexing ferrocene or its derivative with a cyclodextrin. The resulting yellow inclusion complex, an intermediate of the redox dye, is then oxidized electrochemically or by a reaction with bilirubin oxidase to form blue redox dyes which exhibit absorption peaks at 620 or 650 nm. Both the reduced form and the oxidized form of the dye are water-soluble.
摘要翻译：铁或铁基化合物可用作适于监测各种酶氧化过程的蓝色氧化还原染料。提出了制备这种染料的方法，其涉及将二茂铁或其衍生物与环糊精络合。所得到的黄色包合络合物，氧化还原染料的中间体然后电化学氧化或通过与胆红素氧化酶的反应形成蓝色氧化还原染料，其在620或650nm处表现出吸收峰。染料的还原形式和氧化形式都是水溶性的。

5. 发明授权

US07232474B2 Process for producing gold nanoparticles 失效
标题翻译：生产金纳米颗粒的方法
公开(公告)号：US07232474B2
公开(公告)日：2007-06-19
申请号：US10886627
申请日：2004-07-09
申请人： Pierre Bouvrette , Yali Liu , John H. T. Luong , Keith B. Male
发明人： Pierre Bouvrette , Yali Liu , John H. T. Luong , Keith B. Male
IPC分类号： B22F9/24
CPC分类号： B01J35/0013 , B22F1/0018 , B22F9/24 , B82Y30/00 , Y10S977/777
摘要： Gold nanoparticles having a particle size of 4-15 nM are synthesized by the reduction of hydrogen tetrachloroaurate (III) trihydrate using a suitable reducing agent such as sodium citrate in the presence of unmodified α, β or γ-cyclodextrin. The particle size is dependent upon the type and concentration of cyclodextrin and the reducing agent concentration. Gold nanoparticles having a particle size of 2-4 nM are produced by the reduction of hydrogen tetrachloroaurate (III) trihydrate using sodium borohydride in the presence of cyclodextrins.
摘要翻译：通过在未改性的α，β或γ-环糊精的存在下，使用合适的还原剂如柠檬酸钠还原四氯牛磺酸（III）三水合物来合成粒径为4-15nM的金纳米颗粒。粒度取决于环糊精的类型和浓度以及还原剂浓度。通过在环糊精存在下使用硼氢化钠还原四氯牛磺酸（III）三水合物来生产粒径为2-4nM的金纳米颗粒。

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