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1. 发明授权

US06368817B1 Identification of salmonella 有权
标题翻译：鉴定沙门氏菌
公开(公告)号：US06368817B1
公开(公告)日：2002-04-09
申请号：US09355066
申请日：1999-07-08
申请人： John David Perry , Michael Ford
发明人： John David Perry , Michael Ford
IPC分类号： C12Q102
CPC分类号： C12Q1/045 , C12Q1/04 , C12Q2334/20 , C12Q2334/52 , G01N2333/255 , G01N2333/924
摘要： A new culture medium for identifying the presence of Salmonella inenterobacteria samples, especially faeces, contains two chromogenic enzyme substrates, one of which is a substrate for &agr;-D-galactosidase, for which Salmonella is positive. The other substrate is one for which Salmonella is negative such as &bgr;-D-galactosidase. The substrates are incorporated into an agar medium. Positive and negative results are found to be readily observable where one of the substrates is an esculetin, preferably a cyclohexenoesculetin compound in the presence of ferric ions, which produces a black color, and the other substrate is an indoxyl compound, for instance, a 5-bromo-4-chloro-3-indolyl compound which produces a green colored enzymic reaction product.
摘要翻译：用于鉴定肠杆菌样品，特别是粪便中沙门氏菌存在的新培养基含有两种显色酶底物，其中一种是用于α-D-半乳糖苷酶的底物，其中沙门氏菌是阳性的。其他底物是沙门氏菌为阴性的底物，如β-D-半乳糖苷酶。将底物并入琼脂培养基中。发现阳性和阴性结果可以容易地观察到，其中一种底物是七叶素，优选在三价铁离子存在下的环己二烯类锡化合物，其产生黑色，另一种底物是吲哚基化合物，例如5 - 溴-4-氯-3-吲哚基化合物，其产生绿色的酶反应产物。

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