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1. 发明授权

US07332341B2 Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells 有权
标题翻译：在单端孢霉烯缺乏丝状真菌突变体细胞中产生异源多肽的方法
公开(公告)号：US07332341B2
公开(公告)日：2008-02-19
申请号：US11095491
申请日：2005-03-31
申请人： John C. Royer , Lynne M. Christanson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
发明人： John C. Royer , Lynne M. Christanson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
IPC分类号： C12N15/74 , C12N1/00 , C07H21/04
CPC分类号： C12N9/0036 , C12N9/0044 , C12N9/2482 , C12N9/88 , C12N15/80 , C12Y302/01008 , C12Y402/03006
摘要： The present invention relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
摘要翻译：本发明涉及包含基因的无标记物修饰的突变体细胞，以及获得和使用这种突变细胞的方法。

2. 发明授权

US06903193B1 Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells 失效
标题翻译：在单端孢霉烯缺乏丝状真菌突变体细胞中产生异源多肽的方法
公开(公告)号：US06903193B1
公开(公告)日：2005-06-07
申请号：US09710760
申请日：2000-11-10
申请人： John C. Royer , Lynne M. Christianson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
发明人： John C. Royer , Lynne M. Christianson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
IPC分类号： C12N9/02 , C12N9/06 , C12N9/24 , C12N9/88 , C12N15/80 , A23J35/78 , C07K1/00 , C12N1/14 , C12N9/00 , C12N15/00
CPC分类号： C12N9/0036 , C12N9/0044 , C12N9/2482 , C12N9/88 , C12N15/80 , C12Y302/01008 , C12Y402/03006
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding the trichodiene synthases.
摘要翻译：本发明涉及产生多肽的方法，包括：（a）在有利于产生多肽的条件下培养亲本丝状真菌细胞的突变体，其中（i）突变细胞包含编码多肽的第一核酸序列多肽和第二核酸序列，其包含参与单端孢霉烯生产的至少一个基因的修饰，和（ii）当在相同条件下培养时，所述突变体产生比亲本丝状真菌细胞少的单端孢霉素; 和（b）从培养基中分离多肽。本发明还涉及丝状真菌细胞的突变体以及获得突变细胞，分离的二恶烯合酶和编码二氢合酶的核酸序列的方法。

3. 发明授权

US06180366B2 Methods for producing heterologous polypeptides in trichothecene-deficient 有权
标题翻译：在单头孢霉烯缺乏症中产生异源多肽的方法
公开(公告)号：US06180366B2
公开(公告)日：2001-01-30
申请号：US09316080
申请日：1999-05-20
申请人： John C. Royer , Lynne M. Christianson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
发明人： John C. Royer , Lynne M. Christianson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
IPC分类号： C12P2106
CPC分类号： C12N9/0036 , C12N9/0044 , C12N9/2482 , C12N9/88 , C12N15/80 , C12Y302/01008 , C12Y402/03006
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
摘要翻译：本发明涉及产生多肽的方法，包括：（a）在有利于产生多肽的条件下培养亲本丝状真菌细胞的突变体，其中（i）突变细胞包含编码多肽的第一核酸序列多肽和第二核酸序列，其包含参与单端孢霉烯生产的至少一个基因的修饰;和（ii）当在相同条件下培养时，所述突变体产生比亲本丝状真菌细胞少的单端孢霉烯; 和（b）从培养基中分离多肽。本发明还涉及丝状真菌细胞的突变体和获得突变细胞的方法。本发明还涉及分离的二恶烯合成酶和分离的编码二萜烯合酶的核酸序列。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生二氢合酶合酶的方法。本发明还涉及包含基因的无标记物修饰的突变体细胞，以及获得和使用这种突变细胞的方法。

4. 发明申请

US20080213901A1 Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells 审中-公开
标题翻译：在单端孢霉烯缺乏丝状真菌突变体细胞中产生异源多肽的方法
公开(公告)号：US20080213901A1
公开(公告)日：2008-09-04
申请号：US12023417
申请日：2008-01-31
申请人： John C. Royer , Lynne M. Christianson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
发明人： John C. Royer , Lynne M. Christianson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
IPC分类号： C12N15/80
CPC分类号： C12N9/0036 , C12N9/0044 , C12N9/2482 , C12N9/88 , C12N15/80 , C12Y302/01008 , C12Y402/03006
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
摘要翻译：本发明涉及生产多肽的方法，其包括：（a）在有利于产生多肽的条件下培养亲本丝状真菌细胞的突变体，其中（i）突变细胞包含编码多肽的第一核酸序列多肽和第二核酸序列，其包含参与单端孢霉烯生产的至少一个基因的修饰;和（ii）当在相同条件下培养时，所述突变体产生比亲本丝状真菌细胞少的单端孢霉烯; 和（b）从培养基中分离多肽。本发明还涉及丝状真菌细胞的突变体和获得突变细胞的方法。本发明还涉及分离的二恶烯合成酶和分离的编码二萜烯合酶的核酸序列。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生二氢合酶合酶的方法。本发明还涉及包含基因的无标记物修饰的突变体细胞，以及获得和使用这种突变细胞的方法。

5. 发明授权

US06893839B1 Methods for producing polypeptides in cyclohexadepsipeptide-deficient cells 有权
标题翻译：在环己缩酚肽缺陷型细胞中产生多肽的方法
公开(公告)号：US06893839B1
公开(公告)日：2005-05-17
申请号：US09482788
申请日：2000-01-13
申请人： Randy M. Berka , Michael W. Rey , Wendy T. Yoder
发明人： Randy M. Berka , Michael W. Rey , Wendy T. Yoder
IPC分类号： C12N15/09 , C07K11/02 , C12N1/15 , C12N9/00 , C12N9/58 , C12N9/88 , C12N15/60 , C12N15/80 , C12P13/04 , C12P21/02 , C12R1/77 , C12N1/16 , C07H21/04 , C07K17/00 , C12N15/00 , C12P21/06
CPC分类号： C12R1/77 , C12N9/88 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a heterologous polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the heterologous polypeptide, wherein (i) the mutant cell comprises a nucleic acid sequence encoding the heterologous polypeptide and (ii) the mutant produces less of the cyclohexadepsipeptide than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the heterologous polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated cyclohexadepsipeptide synthetases and isolated nucleic acid sequences encoding the cyclohexadepsipeptide synthetases. The present invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the cyclohexadepsipeptide synthetases. The present invention further relates to cyclohexadepsipeptides produced by the cyclohexadepsipeptide synthetases.
摘要翻译：本发明涉及用于产生异源多肽的方法，其包括：（a）在有利于产生异源多肽的条件下培养亲本丝状真菌细胞的突变体，其中（i）突变细胞包含编码当在相同条件下培养时，异源多肽和（ii）突变体比母体丝状真菌细胞产生较少的环己缩酚酸肽; 和（b）从培养基中分离异源多肽。本发明还涉及丝状真菌细胞的突变体和获得突变细胞的方法。本发明还涉及分离的环己缩酚肽合成酶和编码环己缩酚酸肽合成酶的分离的核酸序列。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生环己缩酚肽合成酶的方法。本发明还涉及由环己缩酚肽合成酶产生的环己缩肽。

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