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1. 发明申请

US20110124066A1 PRODUCTION OF ITACONIC ACID 有权
标题翻译：生产酸性酸
公开(公告)号：US20110124066A1
公开(公告)日：2011-05-26
申请号：US12918314
申请日：2009-02-16
申请人： Johannes Petrus Maria Jore , Peter Jan Punt , Maria Johanna Van Der Werf
发明人： Johannes Petrus Maria Jore , Peter Jan Punt , Maria Johanna Van Der Werf
IPC分类号： C12P7/46 , C07H21/04 , C07K14/38
CPC分类号： C12N15/80 , C07K14/38 , C12P7/44
摘要： The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbours other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.
摘要翻译：本发明涉及编码可用于微生物中衣康酸生产的曲霉线粒体三羧酸转运蛋白的核酸序列。所述转运蛋白优选是由位于A.rereus的染色体片段上的核酸编码的蛋白，其还含有参与衣康酸生物合成和洛伐他汀生物合成的其它基因。而且，载体，宿主和转化的微生物是本发明的一部分。

2. 发明授权

US08440436B2 Production of itaconic acid 有权
标题翻译：衣康酸的生产
公开(公告)号：US08440436B2
公开(公告)日：2013-05-14
申请号：US12669955
申请日：2008-07-21
申请人： Maria Johanna Van Der Werf , Martinus Petrus Maria Caspers , Nicole Van Luijk , Peter Jan Punt
发明人： Maria Johanna Van Der Werf , Martinus Petrus Maria Caspers , Nicole Van Luijk , Peter Jan Punt
IPC分类号： C12P7/46 , C12N9/00 , C12N9/88 , C12N15/00 , C07H21/04
CPC分类号： C12N9/88 , C12P7/46
摘要： The invention relates to the production of itaconic acid in micro-organisms by introducting into a suitable host cell a gene coding for the enzyme cis-aconitic acid decarboxylase, preferably derived from A. terreus. Further part of the invention are host cells, preferably from A. niger, provided with the above mentioned genes.
摘要翻译：本发明涉及通过向合适的宿主细胞中引入编码优选来源于土曲霉的顺式乌头酸脱羧酶的基因而在微生物中生产衣康酸。本发明的另一部分是具有上述基因的宿主细胞，优选来自黑曲霉。

3. 发明授权

US08642298B2 Production of itaconic acid 有权
标题翻译：衣康酸的生产
公开(公告)号：US08642298B2
公开(公告)日：2014-02-04
申请号：US12920461
申请日：2009-03-04
申请人： Maria Johanna Van Der Werf , Peter Jan Punt
发明人： Maria Johanna Van Der Werf , Peter Jan Punt
IPC分类号： C12P7/44 , C12N15/31
CPC分类号： C12N15/80 , C07K14/38 , C12P7/44
摘要： The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of Aspergillus terreus or homologues thereof. Overexpression of the protein enhances the production of itaconic acid in microorganisms.
摘要翻译：本发明涉及编码衣康酸输送主要促进剂超家族转运蛋白（MFST）基因序列的核酸序列和由此编码的蛋白质。优选地，所述序列是包含Aspergillus terreus的ATEG_09972.1或其同系物的序列的核酸。蛋白质的过表达增强了微生物中衣康酸的生成。

4. 发明申请

US20100311132A1 PRODUCTION OF ITACONIC ACID 有权
标题翻译：生产酸性酸
公开(公告)号：US20100311132A1
公开(公告)日：2010-12-09
申请号：US12669955
申请日：2008-07-21
申请人： Maria Johanna Van Der Werf , Martinus Petrus Maria Caspers , Nicole Van Luijk , Peter Jan Punt
发明人： Maria Johanna Van Der Werf , Martinus Petrus Maria Caspers , Nicole Van Luijk , Peter Jan Punt
IPC分类号： C12P7/46 , C12N9/88 , C12N1/00 , C12N15/63 , C07H21/04
CPC分类号： C12N9/88 , C12P7/46
摘要： The invention relates to the production of itaconic acid in micro-organisms by introducting into a suitable host cell a gene coding for the enzyme cis-aconitic acid decarboxylase, preferably derived from A. terreus. Further part of the invention are host cells, preferably from A. niger, provided with the above mentioned genes.
摘要翻译：本发明涉及通过向合适的宿主细胞中引入编码优选来源于土曲霉的顺式乌头酸脱羧酶的基因而在微生物中生产衣康酸。本发明的另一部分是具有上述基因的宿主细胞，优选来自黑曲霉。

5. 发明授权

US08679801B2 Production of itaconic acid 有权
标题翻译：衣康酸的生产
公开(公告)号：US08679801B2
公开(公告)日：2014-03-25
申请号：US12918314
申请日：2009-02-16
申请人： Peter Jan Punt , Maria Johanna Van Der Werf
发明人： Peter Jan Punt , Maria Johanna Van Der Werf
IPC分类号： C12P7/44 , C12P7/46 , C12N1/20 , C07K14/00 , C07H21/04
CPC分类号： C12N15/80 , C07K14/38 , C12P7/44
摘要： The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbors other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.
摘要翻译：本发明涉及编码可用于微生物中衣康酸生产的曲霉线粒体三羧酸转运蛋白的核酸序列。所述转运蛋白优选是由位于A.rereus的染色体片段上的核酸编码的蛋白，其还含有参与衣康酸生物合成和洛伐他汀生物合成的其它基因。而且，载体，宿主和转化的微生物是本发明的一部分。

6. 发明申请

US20110070616A1 PRODUCTION OF ITACONIC ACID 有权
标题翻译：生产酸性酸
公开(公告)号：US20110070616A1
公开(公告)日：2011-03-24
申请号：US12920461
申请日：2009-03-04
申请人： Maria Johanna Van Der Werf , Peter Jan Punt
发明人： Maria Johanna Van Der Werf , Peter Jan Punt
IPC分类号： C12P7/44 , C07H21/00 , C07K14/38 , C12N1/15
CPC分类号： C12N15/80 , C07K14/38 , C12P7/44
摘要： The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of Aspergillus terreus or homologues thereof. Overexpression of the protein enhances the production of itaconic acid in micro-organisms.
摘要翻译：本发明涉及编码衣康酸输送主要促进剂超家族转运蛋白（MFST）基因序列的核酸序列和由此编码的蛋白质。优选地，所述序列是包含Aspergillus terreus的ATEG_09972.1或其同系物的序列的核酸。蛋白质的过表达增强了微生物中衣康酸的生成。

7. 发明申请

US20130143271A1 NOVEL EXPRESSION-REGULATING SEQUENCES AND EXPRESSION PRODUCTS IN THE FIELD OF FILAMENTOUS FUNGI 审中-公开
标题翻译： FILAMENTOUS FUNGI领域的新型表达调控序列和表达产物
公开(公告)号：US20130143271A1
公开(公告)日：2013-06-06
申请号：US13046772
申请日：2011-03-14
申请人： Mark Aaron Emalfarb , Peter Jan Punt , Cornelia Maria Johanna Van Zeijl
发明人： Mark Aaron Emalfarb , Peter Jan Punt , Cornelia Maria Johanna Van Zeijl
IPC分类号： C12N9/24 , C12N9/00
CPC分类号： C12N9/248 , C12N9/0008 , C12N9/24 , C12N9/2437 , C12Y302/01004 , C12Y302/01008 , C12Y302/01091
摘要： The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.
摘要翻译：本发明涉及对应于家族7和10的Chrysosporium糖基水解酶的新型蛋白质，其分别与SEQ ID No.2和4的氨基酸序列具有70和75％的最小氨基酸同一性，并且与对应于丙三酸甘油醛磷酸脱氢酶与SEQ ID No.6的部分氨基酸序列呈现至少86％的氨基酸同一性。本发明还涉及编码这些蛋白质的核酸序列，特别是涉及调控相应基因表达的启动子序列。用于表达这些基因的优选宿主是真菌，特别是Chrysosporium菌株。

8. 发明申请

US20120088273A1 NOVEL CELLULOSE AND LIGNO-CELLULOSE ACTIVE PROTEINS 有权
公开(公告)号：US20120088273A1
公开(公告)日：2012-04-12
申请号：US13257255
申请日：2010-03-17
申请人： Peter Jan Punt
发明人： Peter Jan Punt
IPC分类号： C12P19/00 , C12N15/56 , C12N15/63 , C12N5/07 , C12N1/19 , C12N1/15 , C12N5/04 , C12N9/42 , C12N1/21
CPC分类号： C12P19/14 , C12P19/02 , C12P2201/00
摘要： The invention relates to a lignocellulose active protein comprising a DUF1996 domain and proteins that additionally comprise a CBM1 domain. Also included in the invention are proteins with a new domain, characterized by the consensus sequence [GA]-[ST]-[IV]-[ILV]-W-[DS]-G-[RIFS]-F-[ND]-[DS]-X (residues 1-12 of SEQ ID NOS:6-7). The invention relates to a lignocellulose active protein comprising a DUF1996 domain and proteins that additionally comprise a CBM1 domain. Also included in the invention are proteins with a new domain, characterized by the consensus sequence [GA]-[ST]-[IV]-[ILV]-W-[DS]-G-[RIFS]-F-[ND]-[DS]-X2[TS]-X2-D-[LIF]-[ND]-K-W-S-W-[GSA]-N-Q-[IV]-[GP]-[PS]-[YW]-X0-4-Q-[YW]-Y-I-H-G-S-X2-[VT]-X2-Y-[ILV]-X[ILV]-S-X2-[FY]-K-N-P-X5-7-Q-G-X-[KR]-I-T [LI]-D-X-[ST]-[AS]-X-W-N-G-Q-[NT]-M-X-R-[IST]-E-L-I-P-Q-T-X6-13-G-X-[KLV]-[FY]-Y-H-F-S-[ILV]-X5-N-A-P-X4-E-H-Q-[ILV]-[AC]-F-F-E-X0-13-S-H-F-T-E-[LM]-K-[YST]-G-W-X0-2-G-X6-33-[LF]-X1-24-I-D-F-[ASD]-X3-8-V-[FL]-[FWY]-X-S-[ENT]-G-X2-5-[AP]-L-X2-4-[AV]-[AV]-X-[PAN]-X3-5-[ANS]-[AT]-[AFS]-[ST]-[DN]-[GS]-[AQ]-D-[FW]-H-{FILV}-G-[EIQV]-L-[ERK]-[ILV]-P-X8-18-E-D-[FWY]-[FY]-[FW]-S-G-[IV]-[FY]-[IV]-E (SEQ ID NOS:6-7) or a sequence that has a high identity with said sequence. These proteins also may or may not include a CBM1 domain. Further part of the invention are nucleic acids coding for these proteins and vectors and transformants with these nucleic acids. The proteins are of use for their own (hemi-)cellulase activity or for enhancing (hemi-)cellulase activity of other enzymes. [TS]-X2-D-[LIF]-[ND]-K-W-S-W-[GSA]-N-Q-[IV]-[GP]-[PS]-[YW]-X0-4-Q-[YW]-Y-I-H-G-S-X2-[VT]-X2-Y-[ILV]-X[ILV]-S-X2-[FY]-K-N-P-X5-7-Q-G-X-[KR]-I-T-[LI]-D-X-[ST]-[AS]-X-W-N-G-Q-[NT]-M-X-R-[IST]-E-L-I-P-Q-T-X6-13-G-X-[KLV]-[FY]-Y-H-F-S-[ILV]-X5-N-A-P-X4-E-H-Q-[ILV]-[AC]-F-F-E-X0-13-S-H-F-T-E-[LM]-K-[YST]-G-W-X0-2-G-X6-33-[LF]-X1-24-I-D-F-[ASD]-X3-8-V-[FL]-[FWY]-X-S-[ENT]-G-X2-5-[AP]-L-X2-4-[AV]-[AV]-X-[PAN]-X3-5-[ANS]-[AT]-[AFS]-[ST]-[DN]-[GS]-[AQ]-D-[FW]-H-{FILV}-G-[EIQV]-L-[ERK]-[ILV]-P-X8-18-E-D-[FWY]-[FY]-[FW]-S-G-[IV]-[FY]-[IV]-E (SEQ ID NO:82) or a sequence that has a high identity with said sequence. These proteins also may or may not include a CBM1 domain. Further part of the invention are nucleic acids coding for these proteins and vectors and transformants with these nucleic acids. The proteins are of use for their own (hemi-)cellulase activity or for enhancing (hemi-)cellulase activity of other enzymes.

9. 发明申请

US20080193969A1 Hemoglobin Overexpression in Fungal Fermentations 审中-公开
标题翻译：真菌发酵中血红蛋白过度表达
公开(公告)号：US20080193969A1
公开(公告)日：2008-08-14
申请号：US11795043
申请日：2006-01-12
申请人： Rob Te Biesebeke , Peter Jan Punt , Cornelis Antonius Maria Jacobus Johannes Van den Hondel , Willem Meindert De Vos
发明人： Rob Te Biesebeke , Peter Jan Punt , Cornelis Antonius Maria Jacobus Johannes Van den Hondel , Willem Meindert De Vos
IPC分类号： C12P35/00 , C12N1/00 , C12P1/02 , C12P19/00 , C12P7/42 , C12P7/50 , C12P7/44 , C12P7/46 , C12P21/04 , C07K14/00 , C12N15/11 , C12P7/56 , C12P13/04 , C12P7/48 , C12P7/64 , C12P7/06 , C12P23/00 , C12P17/00
CPC分类号： C07K14/805 , C12N9/0061 , C12N9/242 , C12N9/2428 , C12N9/62 , C12P1/02 , Y02E50/17
摘要： The present invention relates to fungal host cells that are transformed with a nucleic acid construct encoding a fungal oxygen-binding proteins or fragments thereof that comprise the oxygen-binding domain. Upon transformation of the host cell with the construct, the oxygen-binding protein confers to the host cell improved fermentation characteristics as compared to untransformed host cells. These characteristics include e.g. increases in oxygen uptake rates, biomass densities, volumetric productivities and/or product yields. The invention further relates to fermentation processes in which the host cells are used and to fungal oxygen binding proteins, in particular fungal flavohemoglobins and hemoglobin domains, and to nucleotides sequences encoding these proteins.
摘要翻译：本发明涉及用编码包含氧结合结构域的真菌氧结合蛋白或其片段的核酸构建体转化的真菌宿主细胞。在用构建体转化宿主细胞后，与未转化的宿主细胞相比，氧结合蛋白赋予宿主细胞改善的发酵特性。这些特征包括例如吸氧速率，生物量密度，体积生产率和/或产品产量的增加。本发明还涉及其中使用宿主细胞和真菌氧结合蛋白，特别是真菌黄血红蛋白和血红蛋白结构域的发酵方法，以及编码这些蛋白质的核苷酸序列。

10. 发明授权

US06573086B1 Transformation system in the field of filamentous fungal hosts 有权
标题翻译：丝状真菌宿主的转化体系
公开(公告)号：US06573086B1
公开(公告)日：2003-06-03
申请号：US09548938
申请日：2000-04-13
申请人： Mark Aaron Emalfrab , Richard Paul Burlingame , Philip Terry Olson , Arkady Panteleimonovich Sinitsyn , Martine Parriche , Jean Christophe Bousson , Christine Marie Pynnonen , Peter Jan Punt , Cornelia Marie Johanna Van Zeijl
发明人： Mark Aaron Emalfrab , Richard Paul Burlingame , Philip Terry Olson , Arkady Panteleimonovich Sinitsyn , Martine Parriche , Jean Christophe Bousson , Christine Marie Pynnonen , Peter Jan Punt , Cornelia Marie Johanna Van Zeijl
IPC分类号： C12N115
CPC分类号： C12N15/80 , C12N9/2437 , C12N9/2462 , C12N9/2482 , C12N9/58 , C12Y302/01004 , C12Y302/01008 , C12Y302/01091
摘要： A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.
摘要翻译：描述了用于表达和分泌异源蛋白质或多肽的丝状真菌宿主领域的新型转化系统。本发明还涵盖以经济方式生产大量多肽或蛋白质的方法。该系统包括转化或转染的Chrysosporium属的真菌菌株，更特别是Chrysosporium lucknowense及其突变体或衍生物。它还包括含有Chrysosporium编码序列的转化体，以及Chrysosporium基因的表达调节序列。还提供了新颖的真菌酶及其编码序列和表达调节序列。

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