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    • 7. 发明授权
    • Mannanase enzymes, genes coding for them and a method for isolating the
genes, as well as a process for bleaching of lignocellulosic pulp
    • 甘露聚糖酶,编码它们的基因和分离基因的方法,以及木质纤维素浆的漂白方法
    • US5661021A
    • 1997-08-26
    • US341568
    • 1994-11-22
    • Johanna BuchertMatti Siika-ahoLiisa ViikariMerja PenttilaAnu SaloheimoMarjatta Ranua
    • Johanna BuchertMatti Siika-ahoLiisa ViikariMerja PenttilaAnu SaloheimoMarjatta Ranua
    • C12N9/24C12N15/56D21C5/00C12N9/42C12N1/14D21C3/00
    • C12Y302/01101C12N9/2488C12Y302/01025C12Y302/01078D21C5/005
    • The present invention relates to a DNA sequence, which codes for endomannanase produced by fungi of the genus Trichoderma and transferred into a yeast or fungus strain induces that strain to produce endomannanase, as well as to a method for isolating genes coding for endomannanases. The invention also relates to vectors, yeast strains and fungal strains containing the DNA sequence. Furthermore, the invention provides an enzyme product containing at least one endomannanase, which contains at least one of the following endomannanases produced by fungi of the Trichoderma genus: an enzyme having mannanase activity and an isoelectric point (pI) of about 3.8, an enzyme having mannanase activity and a pI of about 4.1, an enzyme having mannanase activity and a pI of about 4.5, an enzyme having mannanase activity and a pI of about 5.4 and an enzyme having mannanase activity and a pI of about 6.5, the isoelectric points being determined by isoelectric focusing. The endomannanase enzyme and enzyme products according to the invention can be used for hydrolyzation of mannopolymers, in particular in connection with bleaching of lignocellulosic pulps.
    • PCT No.PCT / FI93 / 00219 Sec。 371日期:1994年11月22日 102(e)1994年11月22日日期PCT提交1993年5月24日PCT公布。 出版物WO93 / 24622 日期:1993年12月9日本发明涉及一种DNA序列,其编码由木霉属真菌产生并转移到酵母或真菌菌株中的内切葡聚糖酶诱导该菌株产生内切葡聚糖酶,以及分离基因编码方法 内分泌酶。 本发明还涉及含有DNA序列的载体,酵母菌株和真菌菌株。 此外,本发明提供一种含有至少一种含有甘露糖醛酸酶的酶产物,其含有由木霉属真菌产生的至少一种以下的甘露聚糖酶:具有约3.8的甘露聚糖酶活性和等电点(pI)的酶,其具有 甘露聚糖酶活性和约4.1的pI,具有约4.5的甘露聚糖酶活性和pI的酶,具有甘露聚糖酶活性和pI约为5.4的酶和具有约6.5的甘露聚糖酶活性和pI的酶,测定等电点 通过等电聚焦。 根据本发明的内切单体酶和酶产物可用于水解甘露聚合物,特别是与木质纤维素纸浆的漂白有关。