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    • 4. 发明授权
    • Materials for enhancing staining of biopolymers in matrices
    • 用于增强基质中生物聚合物染色的材料
    • US07144738B2
    • 2006-12-05
    • US11094255
    • 2005-03-31
    • Scott Whitney
    • Scott Whitney
    • G01N33/68
    • G01N33/542C12Q1/68G01N1/30G01N27/44721G01N27/44726Y10S435/81Y10T436/10Y10T436/143333Y10T436/24Y10T436/25C12Q2565/125C12Q2563/173C12Q2527/125
    • Methods for detecting biopolymers such as proteins, peptides, and nucleic acids in a matrix are provided, in which the methods include contacting the matrix with a sensitizing reagent that includes a substituted heteroaromatic compound; contacting the matrix with a reduceable metal salt to stain the biopolymer(s); and detecting the stained biopolymer(s). Compositions for performing the methods can include sensitizing agents, staining reagents, reducing agents, enhancing agents, and destaining agents. The compositions presented are compatible with mass spectrometry, and methods are provided for staining a biopolymer using the compositions of the invention and performing mass spectrometry on one or more biopolymers identified by the staining methods. Kits for staining biopolymers in matrixes can include a sensitizing reagent and a reduceable metal salt, and optionally, one or more enhancing agents, reducing agents, destaining agents, fixing reagents, chelating agents, biopolymers, matrices, cleavage reagents, buffers, or pH indicators.
    • 提供了用于检测基质中生物聚合物如蛋白质,肽和核酸的方法,其中所述方法包括使基质与包含取代的杂芳族化合物的敏化试剂接触; 使基质与可还原金属盐接触以染色生物聚合物; 并检测染色的生物聚合物。 用于进行该方法的组合物可以包括敏化剂,染色试剂,还原剂,增强剂和脱色剂。 所提供的组合物与质谱法相容,提供了使用本发明组合物对生物聚合物进行染色的方法,并对通过染色方法鉴定的一种或多种生物聚合物进行质谱分析。 在基质中染色生物聚合物的试剂盒可以包括敏化试剂和可还原金属盐,以及任选的一种或多种增强剂,还原剂,脱色剂,固定剂,螯合剂,生物聚合物,基质,裂解试剂,缓冲液或pH指示剂 。
    • 6. 发明申请
    • System, method and computer medium for trading interface
    • US20050004852A1
    • 2005-01-06
    • US10613515
    • 2003-07-03
    • Scott Whitney
    • Scott Whitney
    • G06Q40/00G06F17/60
    • G06Q40/04G06Q40/00
    • What is disclosed is a computer-implemented method for generating a right transfer instruction regarding a transferable right in a priced item. The process includes the steps of graphically presenting a chart of a price of the priced item versus time; and setting a trigger control overlaid on the chart that is responsive to the price to initiate the right transfer instruction when the price reaches a predetermined relationship to a particular price for the priced item. An apparatus includes a communications system for receiving and processing a series of price-indicators for one or more priced items; a presentation system for displaying the series of price-indicators in a graphic format; and a controller, coupled to the communications system, for overlaying one or more interface controls on the series of price-indicators, the controller responsive to user input instructions directed to the one or more overlaying controls for setting a parameter of the one or more controls and for initiating a right transfer process when one or more of price-indicators has a predetermined relationship to the parameter of the one or more controls. An article of manufacture, comprising a program storage medium readable by a computer and embodying one or more instructions executable by the computer for generating a right transfer instruction regarding a transferable right in a priced item presented by the computer, the computer having a computer program application that displays a graphical representation of price history of the priced item, the program storage medium including computer code including I/O access commands, wherein: computer program code presenting graphically a chart of a price of the priced item versus time; and setting a trigger control, using the I/O access commands, overlaid on the chart that is responsive to the price to initiate the right transfer instruction when the price reaches a predetermined relationship to a particular price for the priced item.
    • 10. 发明申请
    • Method for treatment of a semisolid material
    • 半固体材料的处理方法
    • US20050034989A1
    • 2005-02-17
    • US10861787
    • 2004-06-04
    • Roumen BogoevScott WhitneyJoseph Amshey
    • Roumen BogoevScott WhitneyJoseph Amshey
    • G01N33/48C07K1/34C12M1/00C12M1/33C12N15/09C12Q1/68G01N1/10G01N1/28G01L1/20
    • C07K1/34Y10T436/25375Y10T436/255Y10T436/2575
    • An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.
    • 用于切割凝胶的设备和方法包括凝胶切割管和网状材料。 网状材料延伸穿过管的端部。 为了使用切碎装置对凝胶进行细分,将凝胶放置在打孔管中的网状材料上,在离心机中将切细管,网状材料和凝胶纺丝,迫使凝胶通过网状材料,使凝胶细分 变成大致均匀的较小碎片。 网状材料可以以嵌套管的形式固定到管。 嵌套管嵌套在回收容器的开口内。 网状材料可以与嵌套管中的条状多孔膜串联放置。 将嵌套管和回收容器离心通过使凝胶通过网状材料将凝胶材料细分成碎片。 凝胶随后落在膜上,并且可以在膜上处理以提取或以其它方式处理凝胶材料中的分析物。