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    • 3. 发明申请
    • Detection of secreted aspartyl proteases from Candida species
    • 检测念珠菌属分泌的天冬氨酰蛋白酶
    • US20070134743A1
    • 2007-06-14
    • US11302975
    • 2005-12-14
    • Erica PhillipsEnrico DiGiammarinoKevin McGrath
    • Erica PhillipsEnrico DiGiammarinoKevin McGrath
    • G01N33/574G01N33/569C12M3/00
    • G01N33/56961C12Q1/37G01N33/558
    • Methods and devices for the detection of proteins secreted by the hyphal growth form of Candida species are disclosed. The disclosed devices may constitute a method for the diagnosis of acute or chronic infections, including candidiasis, caused by microorganisms of the species Candida, such as C. albicans, for example. The devices of the present invention incorporate antibodies specific to secreted aspartyl protease proteins whose expression is upregulated upon the conversion of the Candida species from the commensal to the pathogenic form. The antibodies may be used in assays to allow the diagnosis of candidal infections and disease conditions. Either monoclonal antibodies or polyclonal antibodies may be used, and in the case of the monoclonals, the specific epitopes of the SAP protein may be detected as well as the SAP protein itself.
    • 公开了用于检测由假丝酵母属菌丝生长形式分泌的蛋白质的方法和装置。 所公开的装置可以构成用于诊断例如由假丝酵母属物种的微生物引起的急性或慢性感染(包括念珠菌病)的方法,例如白色念珠菌。 本发明的装置包含对分泌的天冬氨酰蛋白酶蛋白特异性的抗体,其在将假丝酵母菌种从共生转化为致病形式时,其表达被上调。 抗体可用于测定以允许诊断念珠菌感染和疾病状况。 可以使用单克隆抗体或多克隆抗体,并且在单克隆的情况下,可以检测SAP蛋白的特异性表位以及SAP蛋白本身。