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    • 3. 发明授权
    • Protein multi-blotting method and device
    • 蛋白多点印迹法及装置
    • US08721860B2
    • 2014-05-13
    • US13282030
    • 2011-10-26
    • Stanley Yung-Ping ChienHiroki Yokota
    • Stanley Yung-Ping ChienHiroki Yokota
    • G01N27/447
    • G01N33/6803
    • A technique for producing multiple protein blots from a single gel, including entering the number ‘n’ membranes to be blotted into microprocessor memory, determining calibration constants for a gel batch, inputting calibration constants into microprocessor memory, and calculating ‘n’ voltage/time profiles for simultaneous blotting. A gel layer from the gel batch is treated with a protein sample, ‘n’ membranes are placed onto the gel layer to yield a gel pack, and the gel pack is placed between an electrode plate maintained at a fixed first voltage and an array of ‘n’ spaced generally parallel electrodes. The voltage to each respective ‘n’ spaced generally parallel electrodes is varied over time according to a respective ‘n’ voltage/time profile to transfer proteins to each respective ‘n’ membranes to yield blotted membranes to yield ‘n’ respective blotted membranes.
    • 一种从单一凝胶产生多种蛋白质印迹的技术,包括输入数字“n”膜以便印刷到微处理器存储器中,确定凝胶批次的校准常数,将校准常数输入到微处理器存储器中,并计算“n”电压/时间 轮廓同时印迹。 用蛋白质样品处理凝胶批次的凝胶层,将n膜置于凝胶层上,得到凝胶组,将凝胶组置于保持固定的第一电压的电极板和 'n'间隔开大致平行的电极。 根据相应的“n”电压/时间曲线,每个相应“n”间隔开的大致平行电极的电压随时间而变化,以将蛋白质转移到每个相应的“n”膜,以产生印迹膜以产生相应的印迹膜。
    • 4. 发明申请
    • PROTEIN MULTI-BLOTTING METHOD AND DEVICE
    • 蛋白多重方法和装置
    • US20120103811A1
    • 2012-05-03
    • US13282030
    • 2011-10-26
    • Stanley Yung-Ping ChienHiroki Yokota
    • Stanley Yung-Ping ChienHiroki Yokota
    • G01N33/559G01N27/447C25B7/00
    • G01N33/6803
    • A technique for producing multiple protein blots from a single gel, including entering the number ‘n’ membranes to be blotted into microprocessor memory, determining calibration constants for a gel batch, inputting calibration constants into microprocessor memory, and calculating ‘n’ voltage/time profiles for simultaneous blotting. A gel layer from the gel batch is treated with a protein sample, ‘n’ membranes are placed onto the gel layer to yield a gel pack, and the gel pack is placed between an electrode plate maintained at a fixed first voltage and an array of ‘n’ spaced generally parallel electrodes. The voltage to each respective ‘n’ spaced generally parallel electrodes is varied over time according to a respective ‘n’ voltage/time profile to transfer proteins to each respective ‘n’ membranes to yield blotted membranes to yield ‘n’ respective blotted membranes.
    • 一种从单一凝胶产生多种蛋白质印迹的技术,包括输入数字“n”膜以便印刷到微处理器存储器中,确定凝胶批次的校准常数,将校准常数输入到微处理器存储器中,并计算“n”电压/时间 轮廓同时印迹。 用蛋白质样品处理凝胶批次的凝胶层,将n膜置于凝胶层上,得到凝胶组,将凝胶组置于保持固定的第一电压的电极板和 'n'间隔开大致平行的电极。 根据相应的“n”电压/时间曲线,每个相应“n”间隔开的大致平行电极的电压随时间而变化,以将蛋白质转移到每个相应的“n”膜以产生印迹膜以产生相应的印迹膜。