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1. 发明授权

US10365213B2 Reliable fluorescence correction method for two-color measurement fluorescence system 有权
公开(公告)号：US10365213B2
公开(公告)日：2019-07-30
申请号：US12997223
申请日：2008-06-11
申请人： Jiang Zhu , Cheng Deng , Guoliang Huang , Shukuan Xu , Jing Cheng
发明人： Jiang Zhu , Cheng Deng , Guoliang Huang , Shukuan Xu , Jing Cheng
IPC分类号： G01N33/48 , G01N33/50 , G01N21/27 , G01N21/64
摘要： Methods and devices use in two-color measurement systems. The methods and devices include methods of making corrections, methods of calculating correction factors, fluorescence scanners, and microarray chips. The said methods and devices enable a user to correct fluorescence intensities for errors caused by the occurrence of FRET and/or cross-talk when two fluorophores are used in two-color fluorescence arrays.

2. 发明申请

US20110301062A1 RELIABLE FLUORESCENCE CORRECTION METHOD FOR TWO-COLOR MEASUREMENT FLUORESCENCE SYSTEM 审中-公开
标题翻译：用于两色度测量荧光系统的可靠荧光校正方法
公开(公告)号：US20110301062A1
公开(公告)日：2011-12-08
申请号：US12997223
申请日：2008-06-11
申请人： Jiang Zhu , Cheng Deng , Guoliang Huang , Shukuan Xu , Jing Cheng
发明人： Jiang Zhu , Cheng Deng , Guoliang Huang , Shukuan Xu , Jing Cheng
IPC分类号： C40B30/10 , C40B50/14 , C40B60/12 , C40B60/14
CPC分类号： G01N21/278 , G01N21/6452 , G01N2021/6421 , G01N2021/6441
摘要： Methods and devices use in two-color measurement systems. The methods and devices include methods of making corrections, methods of calculating correction factors, fluorescence scanners, and microarray chips. The said methods and devices enable a user to correct fluorescence intensities for errors caused by the occurrence of FRET and /or cross-talk when two fluorophores are used in two-color fluorescence arrays.
摘要翻译：方法和设备用于双色测量系统。方法和装置包括进行校正的方法，计算校正因子的方法，荧光扫描仪和微阵列芯片。所述方法和装置使得用户能够在双色荧光阵列中使用两个荧光团时，校正由FRET和/或串扰的发生引起的误差的荧光强度。

3. 发明申请

US20090251155A1 On-chip cell migration detection 有权
标题翻译：片上细胞迁移检测
公开(公告)号：US20090251155A1
公开(公告)日：2009-10-08
申请号：US12288971
申请日：2008-10-24
申请人： Lei Wang , Jing Zhu , Cheng Deng , Jing Cheng
发明人： Lei Wang , Jing Zhu , Cheng Deng , Jing Cheng
IPC分类号： G01R27/00
CPC分类号： G01N33/5029 , B01L3/502761 , B01L2200/10 , B01L2200/12 , B01L2300/0636 , B01L2300/0645 , B01L2300/069 , B01L2300/0816 , B01L2300/16 , G01N33/5438
摘要： Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.
摘要翻译：公开了用于检测阻抗的技术，系统和装置。一方面，微电极感测装置包括基板和形成在基板上的微电极传感器阵列。每个传感器包括形成在衬底上的至少一个导电层，并被图案化以包括对电极和多个感测电极，以在不存在和存在位于每个感测的表面的至少一部分上的一个或多个靶单元的情况下检测电信号电极。感测电极间隔开并布置在对电极周围，以提供检测到的电信号的空间平均值。

4. 发明授权

US08779779B2 On-chip cell migration detection 有权
标题翻译：片上细胞迁移检测
公开(公告)号：US08779779B2
公开(公告)日：2014-07-15
申请号：US12288971
申请日：2008-10-24
申请人： Lei Wang , Jing Zhu , Cheng Deng , Jing Cheng
发明人： Lei Wang , Jing Zhu , Cheng Deng , Jing Cheng
IPC分类号： G01R27/28
CPC分类号： G01N33/5029 , B01L3/502761 , B01L2200/10 , B01L2200/12 , B01L2300/0636 , B01L2300/0645 , B01L2300/069 , B01L2300/0816 , B01L2300/16 , G01N33/5438
摘要： Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.
摘要翻译：公开了用于检测阻抗的技术，系统和装置。一方面，微电极感测装置包括基板和形成在基板上的微电极传感器阵列。每个传感器包括形成在衬底上的至少一个导电层，并被图案化以包括对电极和多个感测电极，以在不存在和存在位于每个感测的表面的至少一部分上的一个或多个靶单元的情况下检测电信号电极。感测电极间隔开并布置在对电极周围，以提供检测到的电信号的空间平均值。

5. 发明申请

US20110159535A1 PREPARATION AND USE OF NUCLEATED RED BLOOD CELL SIMULATING PARTICLES AND HEMATOLOGY CONTROL MIXTURES 有权
标题翻译：核红细胞模拟颗粒和血液学控制混合物的制备和使用
公开(公告)号：US20110159535A1
公开(公告)日：2011-06-30
申请号：US12975053
申请日：2010-12-21
申请人： Cheng Deng , Zuyue Xu , He Liu
发明人： Cheng Deng , Zuyue Xu , He Liu
IPC分类号： C12Q1/04 , C12N5/078 , C12N5/0787 , G01N1/28
CPC分类号： G01N33/5094 , G01N21/6428 , G01N33/80 , G01N33/96 , G01N2800/22 , Y10T436/101666
摘要： The present disclosure provides a nucleated red blood cell simulating particle, which may be leukocytes bound to a fluorescent-staining inhibitor capable of stably binding to the nucleus or a nucleic acid in a cell so as to reduce the binding capacity of the particles to a fluorescent dye during their detection. The present disclosure also provides a method for preparing nucleated red blood cell simulating particles, including the following steps: (a) obtaining purified leukocytes; (b) suspending the leukocytes in a cell treatment solution containing a fluorescent-staining inhibitor which stably binds to the nucleus or a nucleic acid in a cell, and (c) washing the obtained product. The present disclosure also provides a hematology control mixture containing the nucleated red blood cell simulating particles. In addition, the present disclosure describes the use of the nucleated red blood cell simulating particles and the hematology control mixtures comprising the same, for the quality control of a blood cell analyzer.
摘要翻译：本公开提供了模拟粒子的有核红细胞，其可以是与能够稳定地结合细胞核的荧光染色抑制剂或细胞中的核酸结合的白细胞，以降低颗粒与荧光的结合能力染料在检测期间。本发明还提供了一种制备有模红细胞模拟颗粒的方法，包括以下步骤：（a）获得纯化的白细胞; （b）将白细胞悬浮于含有稳定结合细胞核的荧光染色抑制剂的细胞处理溶液或细胞中的核酸上，（c）洗涤所得产物。本公开还提供了含有模拟红细胞模拟颗粒的血液控制混合物。此外，本公开内容描述了用于血细胞分析仪的质量控制的模拟有核红细胞模拟颗粒的使用和包含其的血液控制混合物的用途。

6. 发明授权

US08647877B2 Preparation and use of nucleated red blood cell simulating particles and hematology control mixtures 有权
标题翻译：有核红细胞模拟颗粒和血液控制混合物的制备和使用
公开(公告)号：US08647877B2
公开(公告)日：2014-02-11
申请号：US12975053
申请日：2010-12-21
申请人： Cheng Deng , Zuyue Xu , He Liu
发明人： Cheng Deng , Zuyue Xu , He Liu
IPC分类号： G01N31/00 , A01N1/02
CPC分类号： G01N33/5094 , G01N21/6428 , G01N33/80 , G01N33/96 , G01N2800/22 , Y10T436/101666
摘要： The present disclosure provides a nucleated red blood cell simulating particle, which may be leukocytes bound to a fluorescent-staining inhibitor capable of stably binding to the nucleus or a nucleic acid in a cell so as to reduce the binding capacity of the particles to a fluorescent dye during their detection. The present disclosure also provides a method for preparing nucleated red blood cell simulating particles, including the following steps: (a) obtaining purified leukocytes; (b) suspending the leukocytes in a cell treatment solution containing a fluorescent-staining inhibitor which stably binds to the nucleus or a nucleic acid in a cell, and (c) washing the obtained product. The present disclosure also provides a hematology control mixture containing the nucleated red blood cell simulating particles. In addition, the present disclosure describes the use of the nucleated red blood cell simulating particles and the hematology control mixtures comprising the same, for the quality control of a blood cell analyzer.
摘要翻译：本公开提供了模拟粒子的有核红细胞，其可以是与能够稳定地结合细胞核的荧光染色抑制剂或细胞中的核酸结合的白细胞，以降低颗粒与荧光的结合能力染料在检测期间。本发明还提供了一种制备有模红细胞模拟颗粒的方法，包括以下步骤：（a）获得纯化的白细胞; （b）将白细胞悬浮于含有稳定结合细胞核的荧光染色抑制剂的细胞处理溶液或细胞中的核酸上，（c）洗涤所得产物。本公开还提供了含有模拟红细胞模拟颗粒的血液控制混合物。此外，本公开内容描述了用于血细胞分析仪的质量控制的模拟有核红细胞模拟颗粒的使用和包含其的血液控制混合物的用途。

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