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1. 发明授权

US07413712B2 Microfluidic rotary flow reactor matrix 有权
标题翻译：微流控旋流反应器基体
公开(公告)号：US07413712B2
公开(公告)日：2008-08-19
申请号：US10837025
申请日：2004-04-30
申请人： Jian Liu , Carl L. Hansen , Stephen R. Quake
发明人： Jian Liu , Carl L. Hansen , Stephen R. Quake
IPC分类号： C12Q1/68 , F16K11/10 , F15C1/04
CPC分类号： B81B3/00 , B01J19/0093 , B01J2219/00783 , B01J2219/00828 , B01J2219/00833 , B01J2219/00891 , B01J2219/0095 , B01L3/5025 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/10 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/088 , B01L2300/123 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , C12Q1/68 , F04B43/043 , G01N2035/00158
摘要： A microfluidic device comprises a matrix of rotary flow reactors. The microfluidic matrix device offers a solution to the “world-to-chip” interface problem by accomplishing two important goals simultaneously: an economy of scale in reagent consumption is achieved, while simultaneously minimizing pipetting steps. N2 independent assays can be performed with only 2N+1 pipetting steps, using a single aliquot of enzyme amortized over all reactors. The chip reduces labor relative to conventional fluid handling techniques by using an order of magnitude less pipetting steps, and reduces cost by consuming two to three orders of magnitude less reagents per reaction. A PCR format has immediate applications in medical diagnosis and gene testing. Beyond PCR, the microfluidic matrix chip provides a universal and flexible platform for biological and chemical assays requiring parsimonious use of precious reagents and highly automated processing.
摘要翻译：微流体装置包括旋转流反应器的基质。微流体矩阵器件通过同时实现两个重要目标来提供“世界到芯片”接口问题的解决方案：实现了试剂消耗的规模经济，同时最大限度地减少了移液步骤。使用在所有反应器中摊销的酶的单等分试样，可以仅使用2N + 1移液步骤进行N 2 - 2独立测定。该芯片相对于常规流体处理技术通过使用少量的移液步骤来减少劳动，并且通过每次反应消耗两到三个数量级的试剂来降低成本。 PCR格式可以在医学诊断和基因检测中立即应用。除了PCR之外，微流体芯片芯片为生物和化学测定提供了通用和灵活的平台，需要简约地使用贵重的试剂和高度自动化的处理。

2. 发明授权

US07250128B2 Method of forming a via in a microfabricated elastomer structure 有权
标题翻译：在微制造的弹性体结构中形成通孔的方法
公开(公告)号：US07250128B2
公开(公告)日：2007-07-31
申请号：US11111264
申请日：2005-04-20
申请人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Jian Liu , Mark L. Adams , Carl L. Hansen
发明人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Jian Liu , Mark L. Adams , Carl L. Hansen
IPC分类号： B29C39/02
CPC分类号： B29C39/02 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6874 , F04B43/043 , F15C5/00 , F16K31/126 , F16K99/0001 , F16K99/0015 , F16K99/0026 , F16K99/0046 , F16K99/0051 , F16K99/0055 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/2174 , Y10T137/2224 , Y10T137/7879 , Y10T137/87877 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译：一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

3. 发明授权

US06929030B2 Microfabricated elastomeric valve and pump systems 有权
标题翻译：微型弹性阀和泵系统
公开(公告)号：US06929030B2
公开(公告)日：2005-08-16
申请号：US09997205
申请日：2001-11-28
申请人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Jian Liu , Mark L. Adams , Carl L. Hansen
发明人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Jian Liu , Mark L. Adams , Carl L. Hansen
IPC分类号： B01L3/00 , B01L7/00 , B01L9/00 , B67D99/00 , B81B1/00 , B81B3/00 , B81C1/00 , C12Q1/68 , F04B43/04 , F15C5/00 , F16K99/00 , F16K31/126
CPC分类号： B29C39/02 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6874 , F04B43/043 , F15C5/00 , F16K31/126 , F16K99/0001 , F16K99/0015 , F16K99/0026 , F16K99/0046 , F16K99/0051 , F16K99/0055 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/2174 , Y10T137/2224 , Y10T137/7879 , Y10T137/87877 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译：一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

4. 发明申请

US20120009663A1 Nucleic Acid Amplification Using Microfludic Devices 有权
标题翻译：使用微灌装置的核酸扩增
公开(公告)号：US20120009663A1
公开(公告)日：2012-01-12
申请号：US12947774
申请日：2010-11-16
申请人： Markus M. Enzelberger , Carl L. Hansen , Jian Liu , Stephen R. Quake , Chiem Ma
发明人： Markus M. Enzelberger , Carl L. Hansen , Jian Liu , Stephen R. Quake , Chiem Ma
IPC分类号： C12M1/40
CPC分类号： C12Q1/68 , B01F5/102 , B01F5/108 , B01F13/0059 , B01F15/06 , B01L3/50273 , B01L3/502738 , B01L7/525 , B01L9/527 , B01L2300/0861 , B01L2300/088 , B01L2300/0883 , B01L2300/0887 , B01L2300/123 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0481 , B01L2400/0655 , C12Q1/6844 , C12Q3/00 , C12Q2565/629
摘要： The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certain devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.
摘要翻译：本发明提供了在各种类型的热循环反应中使用该微流体装置和方法的微流体装置和方法。某些装置包括旋转微流体通道和沿着旋转微流体通道的不同位置处的多个温度区域，在该温度区域调节温度。溶液可以重复通过温度区域，使得溶液暴露于不同的温度。其他微流体装置包括通过垂直和水平流动通道相交形成的反应室阵列，具有调节反应室温度的能力。微流体装置可用于进行许多不同的分析，包括各种引物延伸反应和核酸扩增反应。

5. 发明授权

US07833708B2 Nucleic acid amplification using microfluidic devices 有权
标题翻译：使用微流控装置进行核酸扩增
公开(公告)号：US07833708B2
公开(公告)日：2010-11-16
申请号：US11133805
申请日：2005-05-19
申请人： Markus M. Enzelberger , Carl L. Hansen , Jian Liu , Stephen R. Quake , Chiem Ma
发明人： Markus M. Enzelberger , Carl L. Hansen , Jian Liu , Stephen R. Quake , Chiem Ma
IPC分类号： C12Q1/68 , C12P19/34 , F16K11/20
CPC分类号： C12Q1/68 , B01F5/102 , B01F5/108 , B01F13/0059 , B01F15/06 , B01L3/50273 , B01L3/502738 , B01L7/525 , B01L9/527 , B01L2300/0861 , B01L2300/088 , B01L2300/0883 , B01L2300/0887 , B01L2300/123 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0481 , B01L2400/0655 , C12Q1/6844 , C12Q3/00 , C12Q2565/629
摘要： The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.
摘要翻译：本发明提供了在各种类型的热循环反应中使用该微流体装置和方法的微流体装置和方法。 Certaom设备包括旋转微流体通道和沿着旋转微流体通道的不同位置处的多个温度区域，在该温度区域调节温度。溶液可以重复通过温度区域，使得溶液暴露于不同的温度。其他微流体装置包括通过垂直和水平流动通道相交形成的反应室阵列，具有调节反应室温度的能力。微流体装置可用于进行许多不同的分析，包括各种引物延伸反应和核酸扩增反应。

6. 发明申请

US20100104477A1 MICROFLUIDIC ROTARY FLOW REACTOR MATRIX 有权
标题翻译：微流控旋流反应器矩阵
公开(公告)号：US20100104477A1
公开(公告)日：2010-04-29
申请号：US12508251
申请日：2009-07-23
申请人： Jian Liu , Carl L. Hansen , Stephen R. Quake
发明人： Jian Liu , Carl L. Hansen , Stephen R. Quake
IPC分类号： B81B3/00
CPC分类号： B81B3/00 , B01J19/0093 , B01J2219/00783 , B01J2219/00828 , B01J2219/00833 , B01J2219/00891 , B01J2219/0095 , B01L3/5025 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/10 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/088 , B01L2300/123 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , C12Q1/68 , F04B43/043 , G01N2035/00158
摘要： A microfluidic device comprises a matrix of rotary flow reactors. The microfluidic matrix device offers a solution to the “world-to-chip” interface problem by accomplishing two important goals simultaneously: an economy of scale in reagent consumption is achieved, while simultaneously minimizing pipetting steps. N2 independent assays can be performed with only 2N+1 pipetting steps, using a single aliquot of enzyme amortized over all reactors. The chip reduces labor relative to conventional fluid handling techniques by using an order of magnitude less pipetting steps, and reduces cost by consuming two to three orders of magnitude less reagents per reaction. A PCR format has immediate applications in medical diagnosis and gene testing. Beyond PCR, the microfluidic matrix chip provides a universal and flexible platform for biological and chemical assays requiring parsimonious use of precious reagents and highly automated processing.
摘要翻译：微流体装置包括旋转流反应器的基质。微流体矩阵器件通过同时实现两个重要目标来提供“世界到芯片”接口问题的解决方案：实现了试剂消耗的规模经济，同时最大限度地减少了移液步骤。可以使用仅在2N + 1移液步骤进行N2独立测定，使用在所有反应器中摊销的单一等分试样。该芯片相对于常规流体处理技术通过使用少量的移液步骤来减少劳动，并且通过每次反应消耗两到三个数量级的试剂来降低成本。 PCR格式可以在医学诊断和基因检测中立即应用。除了PCR之外，微流体芯片芯片为生物和化学测定提供了通用和灵活的平台，需要简约地使用贵重的试剂和高度自动化的处理。

7. 发明申请

US20090087356A1 Microfluidic Rotary Flow Reactor Matrix 审中-公开
标题翻译：微流控旋转反应堆矩阵
公开(公告)号：US20090087356A1
公开(公告)日：2009-04-02
申请号：US12192806
申请日：2008-08-15
申请人： Jian Liu , Carl L. Hansen , Stephen R. Quake
发明人： Jian Liu , Carl L. Hansen , Stephen R. Quake
IPC分类号： B01J19/00
CPC分类号： B81B3/00 , B01J19/0093 , B01J2219/00783 , B01J2219/00828 , B01J2219/00833 , B01J2219/00891 , B01J2219/0095 , B01L3/5025 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/10 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/088 , B01L2300/123 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , C12Q1/68 , F04B43/043 , G01N2035/00158
摘要： A microfluidic device comprises a matrix of rotary flow reactors. The microfluidic matrix device offers a solution to the “world-to-chip” interface problem by accomplishing two important goals simultaneously: an economy of scale in reagent consumption is achieved, while simultaneously minimizing pipetting steps. N2 independent assays can be performed with only 2N+1 pipetting steps, using a single aliquot of enzyme amortized over all reactors. The chip reduces labor relative to conventional fluid handling techniques by using an order of magnitude less pipetting steps, and reduces cost by consuming two to three orders of magnitude less reagents per reaction. A PCR format has immediate applications in medical diagnosis and gene testing. Beyond PCR, the microfluidic matrix chip provides a universal and flexible platform for biological and chemical assays requiring parsimonious use of precious reagents and highly automated processing.
摘要翻译：微流体装置包括旋转流反应器的基质。微流体矩阵器件通过同时实现两个重要目标来提供“世界到芯片”接口问题的解决方案：实现了试剂消耗的规模经济，同时最大限度地减少了移液步骤。可以使用仅在2N + 1移液步骤进行N2独立测定，使用在所有反应器中摊销的单一等分试样。该芯片相对于常规流体处理技术通过使用少量的移液步骤来减少劳动，并且通过每次反应消耗两到三个数量级的试剂来降低成本。 PCR格式可以在医学诊断和基因检测中立即应用。除了PCR之外，微流体芯片芯片为生物和化学测定提供了通用和灵活的平台，需要简约地使用贵重的试剂和高度自动化的处理。

8. 发明授权

US07964139B2 Microfluidic rotary flow reactor matrix 有权
标题翻译：微流控旋流反应器基体
公开(公告)号：US07964139B2
公开(公告)日：2011-06-21
申请号：US12508251
申请日：2009-07-23
申请人： Jian Liu , Carl L. Hansen , Stephen R. Quake
发明人： Jian Liu , Carl L. Hansen , Stephen R. Quake
IPC分类号： B81B3/00
CPC分类号： B81B3/00 , B01J19/0093 , B01J2219/00783 , B01J2219/00828 , B01J2219/00833 , B01J2219/00891 , B01J2219/0095 , B01L3/5025 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/10 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/088 , B01L2300/123 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , C12Q1/68 , F04B43/043 , G01N2035/00158
摘要： A microfluidic device comprises a matrix of rotary flow reactors. The microfluidic matrix device offers a solution to the “world-to-chip” interface problem by accomplishing two important goals simultaneously: an economy of scale in reagent consumption is achieved, while simultaneously minimizing pipetting steps. N2 independent assays can be performed with only 2N+1 pipetting steps, using a single aliquot of enzyme amortized over all reactors. The chip reduces labor relative to conventional fluid handling techniques by using an order of magnitude less pipetting steps, and reduces cost by consuming two to three orders of magnitude less reagents per reaction. A PCR format has immediate applications in medical diagnosis and gene testing. Beyond PCR, the microfluidic matrix chip provides a universal and flexible platform for biological and chemical assays requiring parsimonious use of precious reagents and highly automated processing.
摘要翻译：微流体装置包括旋转流反应器的基质。微流体矩阵器件通过同时实现两个重要目标来提供“世界到芯片”接口问题的解决方案：实现了试剂消耗的规模经济，同时最大限度地减少了移液步骤。可以使用仅在2N + 1移液步骤进行N2独立测定，使用在所有反应器中摊销的单一等分试样。该芯片相对于常规流体处理技术通过使用少量的移液步骤来减少劳动，并且通过每次反应消耗两到三个数量级的试剂来降低成本。 PCR格式可以在医学诊断和基因检测中立即应用。除了PCR之外，微流体芯片芯片为生物和化学测定提供了通用和灵活的平台，需要简约地使用贵重的试剂和高度自动化的处理。

9. 发明授权

US08709153B2 Microfludic protein crystallography techniques 失效
标题翻译：微氟蛋白晶体学技术
公开(公告)号：US08709153B2
公开(公告)日：2014-04-29
申请号：US13280276
申请日：2011-10-24
申请人： Carl L. Hansen , Stephen R. Quake , James M. Berger
发明人： Carl L. Hansen , Stephen R. Quake , James M. Berger
IPC分类号： C30B7/14
CPC分类号： C30B7/14 , F17D5/005 , F17D5/06 , Y10T137/0318 , Y10T137/0391
摘要： The present invention relates to microfluidic devices and methods facilitating the growth and analysis of crystallized materials such as proteins. In accordance with one embodiment, a crystal growth architecture is separated by a permeable membrane from an adjacent well having a much larger volume. The well may be configured to contain a fluid having an identity and concentration similar to the solvent and crystallizing agent employed in crystal growth, with diffusion across the membrane stabilizing that process. Alternatively, the well may be configured to contain a fluid having an identity calculated to affect the crystallization process. In accordance with the still other embodiment, the well may be configured to contain a material such as a cryo-protectant, which is useful in protecting the crystalline material once formed.
摘要翻译：本发明涉及促进结晶物质如蛋白质生长和分析的微流体装置和方法。根据一个实施例，晶体生长结构由具有大得多体积的相邻阱的可渗透膜分离。该孔可以被配置成包含具有与晶体生长中使用的溶剂和结晶剂相似的特性和浓度的流体，并且通过膜的扩散稳定了该过程。或者，孔可以被配置为包含具有计算以影响结晶过程的同一性的流体。根据另一个实施方案，所述孔可以被配置为容纳诸如冷冻保护剂的材料，其可用于在形成后保护结晶材料。

10. 发明授权

US08021480B2 Microfluidic free interface diffusion techniques 有权
标题翻译：微流体界面扩散技术
公开(公告)号：US08021480B2
公开(公告)日：2011-09-20
申请号：US12762170
申请日：2010-04-16
申请人： Carl L. Hansen , Stephen R. Quake , James M. Berger
发明人： Carl L. Hansen , Stephen R. Quake , James M. Berger
IPC分类号： C30B29/02
CPC分类号： C30B7/14 , B01F5/00 , B01F13/0093 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502738 , B01L3/502746 , B01L3/502769 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B81B1/00 , C30B29/02 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0086 , Y10T117/1004 , Y10T117/1008 , Y10T117/1012 , Y10T137/0318 , Y10T137/0324 , Y10T137/2224 , Y10T436/25
摘要： A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.
摘要翻译：将静态流体和第二流体沿微流体界面放置接触，并允许通过扩散混合而不对流流过该界面。根据本发明的一个实施例，流体是静态的，并且最初位于微流体通道中的封闭阀结构的任一侧上，该微流体通道的宽度被紧紧地约束在至少一个维度上。然后打开阀门，并且微流体通道两侧的防滑层抑制沿着所得界面的两种流体之间的对流混合。根据本发明的实施方案的用于微流体自由界面的应用包括但不限于蛋白质结晶研究，蛋白质溶解度研究，流体系统的性质的确定以及各种生物测定如扩散免疫测定，底物转换测定和竞争性结合测定。

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