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1. 发明授权

US08785169B2 Cutinase-producing genetically engineered microorganism and use thereof 有权
标题翻译：生产角质酶的基因工程微生物及其用途
公开(公告)号：US08785169B2
公开(公告)日：2014-07-22
申请号：US13264560
申请日：2011-02-28
申请人： Jian Chen , Jing Wu , Dan Wu , Lei Wang
发明人： Jian Chen , Jing Wu , Dan Wu , Lei Wang
IPC分类号： C12N9/18 , C12N1/20
CPC分类号： C12N9/18 , C12Y301/01074
摘要： The present invention relates to the field of bioengineering and discloses a cutinase-producing genetically engineered microorganism and use thereof. Recombinant plasmid Tfu_0883-hlyAs/pET20b(+) was constructed and transformed into E. coli BL21(DE3) to obtain recombinant E. coli strain Tfu_0883-hlyAs/pET20b(+)/E. coli BL21(DE3). Specific growth rate was maintained at a certain value using fed-batch fermentation mode. After fermenting 30-34 hours, the enzyme activity in the supernatant reached 700-750 U/mL. The present invention uses glycerol as the main raw material and employs semi-synthetic medium, has the advantages of good stability and ease of control, and is suitable for large-scale production.
摘要翻译：本发明涉及生物工程领域，并公开了一种生产基因工程转基因的微生物及其用途。构建重组质粒Tfu_0883-hlyAs / pET20b（+），转化大肠杆菌BL21（DE3），得到重组大肠杆菌菌株Tfu_0883-hlyAs / pET20b（+）/ E。大肠杆菌BL21（DE3）。使用补料分批发酵模式，比生长速率保持在一定值。发酵30-34小时后，上清液中酶活力达到700〜750U / mL。本发明以甘油为主要原料，采用半合成培养基，具有稳定性好，易控制的优点，适合大规模生产。

2. 发明申请

US20120149086A1 Cutinase-Producing Genetically Engineered Microorganism and Use Thereof 有权
标题翻译：角质酶生产基因工程微生物及其应用
公开(公告)号：US20120149086A1
公开(公告)日：2012-06-14
申请号：US13264560
申请日：2011-02-28
申请人： Jian Chen , Jing Wu , Dan Wu , Lei Wang
发明人： Jian Chen , Jing Wu , Dan Wu , Lei Wang
IPC分类号： C12N9/18 , C12N1/21
CPC分类号： C12N9/18 , C12Y301/01074
摘要： The present invention relates to the field of bioengineering and discloses a cutinase-producing genetically engineered microorganism and use thereof. Recombinant plasmid Tfu_0883-hlyAs/pET20b(+) was constructed and transformed into E. coli BL21(DE3) to obtain recombinant E. coli strain Tfu_0883-hlyAs/pET20b(+)/E. coli BL21(DE3). Specific growth rate was maintained at a certain value using fed-batch fermentation mode. After fermenting 30-34 hours, the enzyme activity in the supernatant reached 700-750 U/mL. The present invention uses glycerol as the main raw material and employs semi-synthetic medium, has the advantages of good stability and ease of control, and is suitable for large-scale production.
摘要翻译：本发明涉及生物工程领域，并公开了一种生产基因工程转基因的微生物及其用途。构建重组质粒Tfu_0883-hlyAs / pET20b（+），转化大肠杆菌BL21（DE3），得到重组大肠杆菌菌株Tfu_0883-hlyAs / pET20b（+）/ E。大肠杆菌BL21（DE3）。使用补料分批发酵模式，比生长速率保持在一定值。发酵30-34小时后，上清液中酶活力达到700〜750U / mL。本发明以甘油为主要原料，采用半合成培养基，具有稳定性好，易控制的优点，适合大规模生产。

3. 发明申请

US20140011239A1 Method for Enhancing Extracellular Secretion of Recombinant Proteins in Escherichia coli by Co-expressing Thermobifida Fusca Cutinase 有权
标题翻译：通过共表达Thermobifida Fusca角质酶在大肠杆菌中增强重组蛋白的细胞外分泌的方法
公开(公告)号：US20140011239A1
公开(公告)日：2014-01-09
申请号：US13935519
申请日：2013-07-04
申请人： Jing Wu , Lingqia Su , Jian Chen
发明人： Jing Wu , Lingqia Su , Jian Chen
IPC分类号： C12N9/20 , C12P21/00
CPC分类号： C12N9/20 , C12N9/2408 , C12N9/2414 , C12N9/2434 , C12N9/248 , C12P21/00 , C12P21/02
摘要： The present invention provides a method of increasing extracellular secretion of secretory proteins by co-expressing the secretory proteins with a mature cutinase. Cutinase can improve the permeability of E. coli cell membrane without destroying the membrane, and thus facilitate cross-membrane transfer of the secretory proteins co-expressed in E. coli. Increased extracellular secretion of target proteins can shorten cell culture time, reduce the formation of inclusion bodies and increase production of target proteins.
摘要翻译：本发明提供了通过用成熟角质酶共表达分泌蛋白来增加分泌蛋白的细胞外分泌的方法。角质酶可以改善大肠杆菌细胞膜的通透性，而不破坏膜，从而促进大肠杆菌中共表达的分泌蛋白的跨膜转移。靶蛋白的细胞外分泌增加可以缩短细胞培养时间，减少包涵体的形成并增加靶蛋白的产生。

4. 外观设计

USD1008676S1 Step-stool 有权
公开(公告)号：USD1008676S1
公开(公告)日：2023-12-26
申请号：US29820654
申请日：2021-12-23
申请人： Jian Chen
设计人： Jian Chen
摘要： FIG. 1 is a perspective view of a step-stool showing my new design;
FIG. 2 is a front view thereof;
FIG. 3 is a rear view thereof;
FIG. 4 is a left side view thereof;
FIG. 5 is a right side view thereof;
FIG. 6 is a top view thereof;
FIG. 7 is a bottom view thereof;
FIG. 8 is a bottom side perspective view thereof; and,
FIG. 9 is a perspective view of the step-stool of FIG. 1, shown with unclaimed patterned ornamentation.
The broken lines in the drawings depict portions of the step-stool that form no part of the claimed design.

5. 发明申请

US20200032191A1 Shock Tube and Cell Electroporation Device with the Shock Tube 审中-公开
公开(公告)号：US20200032191A1
公开(公告)日：2020-01-30
申请号：US16586989
申请日：2019-09-29
申请人： Jian Chen
发明人： Jian Chen
IPC分类号： C12M1/42 , C12M1/12 , B01L3/00
摘要： The shock tube comprises a tube, a first electrode, a second electrode and a stopple, wherein the tube is internally provided with a cavity for accommodating a target liquid sample. The first electrode is arranged at one end of the tube. The second electrode is arranged in the stopple, and the outer end of the second electrode can be electrically connected with the exterior via an opening of the stopple. The stopple is internally provided with an elastic piece connected with the second electrode. The outer side of the elastic piece is connected with the stopple, and the inner side of the elastic piece is connected with the second electrode. The invention further provides a cell electroporation device where the shock tube can be placed.

6. 发明授权

US10392634B2 Method for high-efficiency production of pinoresinol using an H2O2 auto-scavenging cascade 有权
公开(公告)号：US10392634B2
公开(公告)日：2019-08-27
申请号：US15472003
申请日：2017-03-28
申请人： Jingwen Zhou , Jian Chen , Yongkun Lv , Guocheng Du
发明人： Jingwen Zhou , Jian Chen , Yongkun Lv , Guocheng Du
IPC分类号： C12P7/22 , C12N9/04 , C12N9/08 , C12N1/20 , C12N15/00 , C07H21/04 , C12N15/62 , C12P17/18
摘要： The present invention provides a method for high-efficiency production of pinoresinol by use of an H2O2 auto-scavenging enzymatic cascade. It uses eugenol as the substrate, which is relatively inexpensive and is industrially available. It uses an enzymatic cascade to remove H2O2 produced in the process of pinoresinol synthesis, thereby reducing its inhibitory effect on the enzyme activity. In addition, the present invention uses whole cells as a catalyst, which can continuously regenerate cofactors needed by the enzyme, thus eliminating the need for exogenous addition of expensive cofactors during the reaction. The yield of the present invention can reach 7.12 g/L and the conversion rate is 61.55%.

7. 发明授权

US10085470B2 Method for improving the quality of soy sauce using Bacillus amyloliquefaciens 有权
公开(公告)号：US10085470B2
公开(公告)日：2018-10-02
申请号：US15204991
申请日：2016-07-07
申请人： Fang Fang , Jiran Zhang , Xifei Yang , Chuanwang Hu , Jian Chen , Guocheng Du
发明人： Fang Fang , Jiran Zhang , Xifei Yang , Chuanwang Hu , Jian Chen , Guocheng Du
IPC分类号： A23L5/20 , A23L27/50 , A23L23/00 , C12N1/20 , C12R1/07
摘要： The present invention provides a method for improving the quality of soy sauce using Bacillus amyloliquefaciens, which relates to the field of microbiology and food technology. The method comprises inoculating Bacillus amyloliquefaciens BBE JY06 during soy sauce fermentation. With Bacillus amyloliquefaciens addition during soy sauce fermentation, the aroma and tastes of soy sauce is improved and the ethyl carbamate content in soy sauce is decreased as well.

8. 发明授权

US09690855B2 Method and system for searching for a web document 有权
公开(公告)号：US09690855B2
公开(公告)日：2017-06-27
申请号：US13549416
申请日：2012-07-14
申请人： Sheng Hua Bao , Jian Chen , Zhong Su , Rui Wang
发明人： Sheng Hua Bao , Jian Chen , Zhong Su , Rui Wang
IPC分类号： G06F17/30
CPC分类号： G06F17/30864
摘要： Provided is a method applied to a system including a plurality of sensors, a proxy, and a server. The method includes: measuring objects by the plurality of sensors to obtain first measurement values; computing a correlation among the first measurement values by the server based on the first measurement values; computing an actual measurement value of a second measurement value by the proxy based on the first measurement values and a given function; verifying the plurality of sensors by setting one or more sensors as verification target sensors in turn by the server at predetermined timing; computing a predicted value of the second measurement value by the server based on the correlation and the first measurement values obtained from the other sensors; and outputting the predicted value of the second measurement value instead of the actual measurement value thereof at least during the verification of the verification target sensors.

9. 发明授权

US09601991B2 Switching regulator control circuit 有权
公开(公告)号：US09601991B2
公开(公告)日：2017-03-21
申请号：US13438398
申请日：2012-04-03
申请人： Jian Chen
发明人： Jian Chen
IPC分类号： H02M3/335 , H02M1/42
CPC分类号： H02M1/4258 , Y02B70/126
摘要： In a switching regulator control circuit according to aspects of the invention, a drain current is converted to a voltage Vis with a resistance. The voltage is delivered to a multiplication circuit. The multiplication circuit generates and outputs a voltage that is a product signal of the voltage and a voltage that is proportional to a duty factor. A comparator circuit compares the voltage with an error signal delivered to the other comparison input terminal of the comparator. When the voltage has reached the error voltage, the comparator delivers a turn-off instruction through an OR circuit to a terminal of a flip-flop.

10. 发明授权

US09390134B2 Regular expression matching method and system, and searching device 有权
标题翻译：正则表达式匹配方法和系统，搜索设备
公开(公告)号：US09390134B2
公开(公告)日：2016-07-12
申请号：US13339043
申请日：2011-12-28
申请人： Rui Hu , Jian Chen
发明人： Rui Hu , Jian Chen
IPC分类号： G06F17/30
CPC分类号： G06F17/30516 , G06F17/30867
摘要： A regular expression matching method and system, and a searching device are provided. First, the searching device performs string filtering on a data stream to be matched, in which if keywords in the data stream and preset character words have at least one same character, the searching device indicates that the data stream passes through the string filtering. Then the searching device performs regular expression filtering on the data stream passing through the string filtering. In a string filtering process through the method, system, and device, when Hash mapping positions of the keywords of the data stream are a subset of the Hash mapping positions of the character words, it indicates that the data stream passes through the string filtering, and it is not required to store the keywords and further compare the keywords with the character words, thereby saving the storage space and improving performance.
摘要翻译：提供了正则表达式匹配方法和系统，以及搜索装置。首先，搜索装置对要匹配的数据流执行字符串过滤，其中如果数据流中的关键字和预设字符字具有至少一个相同的字符，则搜索装置指示数据流通过字符串过滤。然后，搜索设备对通过字符串过滤的数据流执行正则表达式过滤。在通过方法，系统和设备的字符串过滤过程中，当数据流的关键字的哈希映射位置是字符字的哈希映射位置的子集时，表示数据流通过字符串过滤，并且不需要存储关键词并进一步将关键词与字符词进行比较，从而节省存储空间并提高性能。

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