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    • 2. 发明授权
    • Blotting method for rapidly analyzing nucleic acid
    • 用于快速分析核酸的印迹方法
    • US07501250B2
    • 2009-03-10
    • US11535478
    • 2006-09-26
    • Chung-Cheng ChangJau-Der ChenPei-Tai Chen
    • Chung-Cheng ChangJau-Der ChenPei-Tai Chen
    • C12Q1/68C07H21/02C07H21/04
    • C12Q1/6837
    • The present invention relates to a blotting method for rapidly analyzing nucleic acid comprising the steps of transferring a nucleic acid to be analyzed to the substrate and fixing the nucleic acid to be analyzed absorbed on the substrate; directing adding a nucleic acid probe to hybridize in a short time, without blocking the areas where the nucleic acid to be analyzed has not been fixed; removing the nucleic acid probe which has not been annealed to the nucleic acid to be analyzed by washing; and finally detecting the hybridization signal. According to the present invention, since the prehybridization is not needed and the hybridization and washing time is shortened, the time for the nucleic acid hybridization is dramatically shortened. Therefore, the whole blotting procedures for rapidly analyzing nucleic acid may be finished quickly.
    • 本发明涉及一种用于快速分析核酸的印迹方法,包括以下步骤:将要分析的核酸转移至底物并将待分析的核酸吸附在基质上; 指导添加核酸探针以在短时间内杂交,而不阻断待分析的核酸未被固定的区域; 通过洗涤除去尚未退火的核酸探针; 最后检测杂交信号。 根据本发明,由于不需要预杂交并且缩短了杂交和洗涤时间,因此大大缩短了核酸杂交的时间。 因此,快速分析核酸的全部印迹方法可以快速完成。
    • 4. 发明申请
    • Blotting Method for Rapidly Analyzing Nucleic Acid
    • 用于快速分析核酸的印迹方法
    • US20080076125A1
    • 2008-03-27
    • US11535478
    • 2006-09-26
    • Chung-Cheng ChangJau-Der ChenPei-Tai Chen
    • Chung-Cheng ChangJau-Der ChenPei-Tai Chen
    • C12Q1/68C12M1/34
    • C12Q1/6837
    • The present invention relates to a blotting method for rapidly analyzing nucleic acid comprising the steps of transferring a nucleic acid to be analyzed to the substrate and fixing the nucleic acid to be analyzed absorbed on the substrate; directing adding a nucleic acid probe to hybridize in a short time, without blocking the areas where the nucleic acid to be analyzed has not been fixed; removing the nucleic acid probe which has not been annealed to the nucleic acid to be analyzed by washing; and finally detecting the hybridization signal. According to the present invention, since the prehybridization is not needed and the hybridization and washing time is shortened, the time for the nucleic acid hybridization is dramatically shortened. Therefore, the whole blotting procedures for rapidly analyzing nucleic acid may be finished quickly.
    • 本发明涉及一种用于快速分析核酸的印迹方法,包括以下步骤:将要分析的核酸转移至底物并将待分析的核酸吸附在基质上; 指导添加核酸探针以在短时间内杂交,而不阻断待分析的核酸未被固定的区域; 通过洗涤除去未被退火的待测核酸的核酸探针; 最后检测杂交信号。 根据本发明,由于不需要预杂交并且缩短了杂交和洗涤时间,因此大大缩短了核酸杂交的时间。 因此,快速分析核酸的全部印迹方法可以快速完成。
    • 5. 发明授权
    • Wheel seat assembly for roller skate
    • 轮滑轮座椅总成
    • US06464236B2
    • 2002-10-15
    • US09732771
    • 2000-12-11
    • Chung-Cheng Chang
    • Chung-Cheng Chang
    • A63C1720
    • A63C17/20A43B5/1633A63C17/008Y10T403/32426
    • A wheel seat assembly of a roller skate includes a sole body fixed on the bottom of a shoe. The sole body is provided with receiving chambers for securing casings therein. The casing defines an axial hole and at least two positioning holes. A roller seat is pivotally mounted in the casing by a pivot member. The pivot member is fitted with a catch member and an elastic member. The catch member may be selectively locked into one of the positioning holes of the casing by an urging action of the elastic member. The roller seat has a roller rotatably mounted therein by a rotation shaft.
    • 溜冰鞋的轮座组件包括固定在鞋底上的鞋底主体。 鞋底本体设置有用于将壳体固定在其中的接收室。 壳体限定轴向孔和至少两个定位孔。 辊座通过枢轴构件枢转地安装在壳体中。 枢转构件装配有卡扣构件和弹性构件。 可以通过弹性部件的推动动作将卡合部件选择性地锁定在壳体的一个定位孔中。 辊座具有通过旋转轴可旋转地安装在其中的辊。
    • 10. 发明申请
    • METHOD OF NUCLEIC ACID HYBRIDIZATION
    • 核酸杂交方法
    • US20080194804A1
    • 2008-08-14
    • US11778251
    • 2007-07-16
    • Chung-Cheng ChangJau-Der ChenPei-Tai Chen
    • Chung-Cheng ChangJau-Der ChenPei-Tai Chen
    • C07H21/02C07H21/04
    • C12Q1/6834C12Q2565/632C12Q2565/631C12Q2565/625
    • The present invention relates to a nucleic acid hybridization method and in particular to a method using a microfluidic chip-based hybridization device with modified procedures which shows the advantages of simplicity and efficiency. The method of the present invention is to mix the test nucleic acid and the probe nucleic acid in advance, feed the solution into a hybridization device with a porous substrate, let the nucleic acid to be analyzed absorb into the substrate. The washing solution is then injected into the same device to wash out the unhybridized probes and the signals of hybridization absorbed on the substrate are detected for analysis. The conventional 2-step approach for the hybridization of the nucleic acid to be analyzed and the probe is combined into one step by the method of the invention provided here, which simplifies the necessary steps and shorten the time needed for hybridization reaction and detection.
    • 本发明涉及核酸杂交方法,特别涉及使用具有改进方法的基于微流体芯片的杂交装置的方法,其显示出简单性和效率的优点。 本发明的方法是预先混合测试核酸和探针核酸,将溶液加入到具有多孔底物的杂交装置中,使待分析的核酸吸收到底物中。 然后将洗涤溶液注射到相同的装置中以洗出未杂交的探针,并检测在底物上吸收的杂交信号用于分析。 通过本文提供的本发明方法将用于杂交待分析的核酸和探针的常规2步法合并成一个步骤,这简化了必要步骤并缩短了杂交反应和检测所需的时间。