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1. 发明申请

US20080145884A1 SFRP AND PEPTIDE MOTIFS THAT INTERACT WITH SFRP AND METHODS OF THEIR USE 有权
标题翻译：与SFRP相互作用的SFRP和肽活动及其使用方法
公开(公告)号：US20080145884A1
公开(公告)日：2008-06-19
申请号：US12019567
申请日：2008-01-24
申请人： Jeffrey S. Rubin , Aykut Oren , Brian K. Kay , Bernard Weisblum , Matthew Tood Gillespie , Nicole Joy Horwood
发明人： Jeffrey S. Rubin , Aykut Oren , Brian K. Kay , Bernard Weisblum , Matthew Tood Gillespie , Nicole Joy Horwood
IPC分类号： C12Q1/42 , C12N5/06 , C07K7/06 , C07K7/08 , C07H21/04 , C12N15/63
CPC分类号： C12N5/0643 , A61K38/00 , C07H21/00 , C07K7/08 , C07K14/001 , C07K14/47 , C07K14/71 , C07K2319/00 , C12N2501/415 , G01N2500/00
摘要： This disclosure relates to a peptide motif and proteins containing the motif that are capable of binding to secreted Frizzled-related protein family members. Accordingly, the disclosure also includes methods of regulating the interaction of sFRP-1 with proteins containing the motif.
摘要翻译：本公开涉及肽基序和含有能够结合分泌的卷曲相关蛋白家族成员的基序的蛋白质。因此，本公开还包括调节sFRP-1与含有该基序的蛋白质的相互作用的方法。

2. 发明授权

US08334260B2 sFRP and peptide motifs that interact with sFRP and methods of their use 有权
标题翻译：与sFRP相互作用的sFRP和肽基序及其使用方法
公开(公告)号：US08334260B2
公开(公告)日：2012-12-18
申请号：US12019567
申请日：2008-01-24
申请人： Jeffrey S. Rubin , Aykut Üren , Brian K. Kay , Bernard Weisblum , Matthew Todd Gillespie , Nicole Joy Horwood
发明人： Jeffrey S. Rubin , Aykut Üren , Brian K. Kay , Bernard Weisblum , Matthew Todd Gillespie , Nicole Joy Horwood
IPC分类号： A61K38/00 , A61K38/16 , C07K7/00
CPC分类号： C12N5/0643 , A61K38/00 , C07H21/00 , C07K7/08 , C07K14/001 , C07K14/47 , C07K14/71 , C07K2319/00 , C12N2501/415 , G01N2500/00
摘要： This disclosure relates to a peptide motif and proteins containing the motif that are capable of binding to secreted Frizzled-related protein family members. Accordingly, the disclosure also includes methods of regulating the interaction of sFRP-1 with proteins containing the motif.
摘要翻译：本公开涉及肽基序和含有能够结合分泌的卷曲相关蛋白家族成员的基序的蛋白质。因此，本公开还包括调节sFRP-1与含有该基序的蛋白质的相互作用的方法。

3. 发明授权

US06184205B2 GRB2 SH3 binding peptides and methods of isolating and using same 失效
标题翻译： GRB2 SH3结合肽及其分离和使用方法
公开(公告)号：US06184205B2
公开(公告)日：2001-02-06
申请号：US08602999
申请日：1996-02-16
申请人： Andrew B. Sparks , Brian K. Kay , Judith M. Thorn , Lawrence A. Quilliam , Channing J. Der , Dana M. Fowlkes , James E. Rider
发明人： Andrew B. Sparks , Brian K. Kay , Judith M. Thorn , Lawrence A. Quilliam , Channing J. Der , Dana M. Fowlkes , James E. Rider
IPC分类号： A61K3810
CPC分类号： C07K7/06 , A61K38/00 , A61K47/62 , C07K7/08 , C07K14/001 , C07K14/435 , C07K2319/00 , C40B30/04 , G01N33/5008 , G01N33/5011 , G01N33/5041 , G01N33/573 , G01N33/68 , G01N33/6845
摘要： Peptides having general and specific binding affinities for the Src homology region 3 (SH3) domains of proteins are disclosed in the present invention. In particular, SH3 binding peptides have been isolated from phage-displayed random peptide libraries which had been screened for isolates that bind to bacterial fusion proteins having an SH3 domain and glutathione S-transferase (GST). Preferred peptides are disclosed which comprise a core 7-mer sequence (preferably, a consensus motif) and two or more, preferably at least six, additional amino acid residues flanking the core sequence, for a total length of 9, preferably at least 13, amino acid residues and no more than about 45 amino acid residues. Such peptides manifest preferential binding affinities for certain SH3 domains. The preferred peptides exhibit specific binding affinities for the Src-family of proteins. In vitro and in vivo results are presented which demonstrate the biochemical activity of such peptides.
摘要翻译：在本发明中公开了对蛋白质的Src同源性区域3（SH3）结构域具有一般和特异性结合亲和力的肽。特别地，从噬菌体展示的随机肽文库中分离出SH3结合肽，其已经筛选结合具有SH3结构域和谷胱甘肽S-转移酶（GST）的细菌融合蛋白的分离物。公开了优选的肽，其包含核心序列侧翼核心7-聚体序列（优选共有基序）和两个或多个，优选至少六个另外的氨基酸残基，总长度为9，优选至少13，氨基酸残基和不超过约45个氨基酸残基。这些肽表现出某些SH3结构域的优先结合亲和力。优选的肽显示出对Src家族蛋白质的特异性结合亲和力。提出体外和体内结果，证明了这些肽的生化活性。

4. 发明授权

US5935823A Totally synthetic affinity reagents 失效
标题翻译：全合成亲和试剂
公开(公告)号：US5935823A
公开(公告)日：1999-08-10
申请号：US420945
申请日：1995-04-11
申请人： Dana M. Fowlkes , Brian K. Kay
发明人： Dana M. Fowlkes , Brian K. Kay
IPC分类号： C07K19/00 , C07K14/00 , C07K16/00 , C07K16/18 , C07K16/30 , C07K16/40 , C07K16/44 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/62 , C12N15/70 , C12P21/02 , C12P21/04 , C12R1/19
CPC分类号： C12N15/1048 , C07K16/00 , C07K16/18 , C07K16/3007 , C07K16/3069 , C07K16/40 , C07K16/44 , C12N15/1075 , C12N15/62 , C12N15/70 , C07K2319/00 , C07K2319/20 , C07K2319/50 , C07K2319/61 , C07K2319/705 , C07K2319/73 , C07K2319/735
摘要： A novel process for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARS) is disclosed. TSARs are concatenated heterofunctional polypeptides or proteins comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional polypeptides or proteins further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.
摘要翻译：公开了一种用于产生称为全合成亲和试剂（TSARS）的新型和/或改进的异型结合融合蛋白的新方法。 TSAR是连接的杂多功能多肽或包含至少两个功能区的蛋白质：对配体具有亲和力的结合结构域和化学或生物活性的第二效应肽部分。在一个实施方案中，杂多官能多肽或蛋白质还包含在结合结构域和第二活性肽部分之间的连接肽部分。接头肽可以是敏感的或不易于通过酶促或化学方式切割。还公开了新型和/或改进的异型结合试剂以及用于多种体外和体内应用的方法。

5. 发明授权

US5596079A Mimetics of senescent cell derived inhibitors of DNA synthesis 失效
标题翻译：衰老细胞衍生的DNA合成抑制剂的模拟物
公开(公告)号：US5596079A
公开(公告)日：1997-01-21
申请号：US249371
申请日：1994-05-24
申请人： James R. Smith , Brian K. Kay
发明人： James R. Smith , Brian K. Kay
IPC分类号： A61K38/00 , C07H21/00 , C07K14/47 , C07K16/18 , C12N15/113 , C07K7/00
CPC分类号： A61K31/70 , B82Y5/00 , C07H21/00 , C07K14/4703 , C07K16/18 , C12N15/113 , A61K38/00 , C07K2319/00 , C12N2310/111 , C12N2310/322
摘要： Phage display methods were used to identify mimetics of SDI-1. Such mimetics are small peptides that are capable of binding to cyclin molecules, especially CDK2. The invention is directed to the mimetics and to methods for producing them.
摘要翻译：使用噬菌体展示方法鉴定SDI-1的模拟物。这种模拟物是能够结合细胞周期蛋白分子，特别是CDK2的小肽。本发明涉及模拟物及其制备方法。

6. 发明授权

US06709821B2 Polypeptides having a functional domain of interest and methods of identifying and using same 失效
标题翻译：具有感兴趣的功能结构域的多肽以及鉴定和使用它们的方法
公开(公告)号：US06709821B2
公开(公告)日：2004-03-23
申请号：US09879957
申请日：2001-06-13
申请人： Andrew B. Sparks , Noah Hoffman , Brian K. Kay , Dana M. Fowlkes , Stephen J. McConnell
发明人： Andrew B. Sparks , Noah Hoffman , Brian K. Kay , Dana M. Fowlkes , Stephen J. McConnell
IPC分类号： C12Q168
CPC分类号： C40B30/04 , A61K38/00 , C12N15/1037 , C40B40/02 , G01N33/6842 , G01N33/6845 , G01N2500/02 , G01N2500/04
摘要： Novel polypeptides having functional domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.
摘要翻译：描述了具有目标功能结构域的新型多肽，以及编码该功能结构域的DNA序列。还公开了通过独立于序列（即，独立于所寻求的多肽的一级序列）鉴定这些多肽的方法，基于识别单元的功能屏幕也被公开。描述了鉴定的方法和多肽的各种应用，包括它们在用于药物发现，修饰和细化的测定试剂盒中的用途。

7. 发明授权

US5625033A Totally synthetic affinity reagents 失效
标题翻译：全合成亲和试剂
公开(公告)号：US5625033A
公开(公告)日：1997-04-29
申请号：US471052
申请日：1995-06-06
申请人： Brian K. Kay , Dana M. Fowlkes
发明人： Brian K. Kay , Dana M. Fowlkes
IPC分类号： C07K14/47 , C07K16/00 , C07K16/18 , C07K16/30 , C07K16/40 , C07K16/44 , C12N15/10 , C12N15/62 , C12N15/70 , C12P21/04 , C40B40/02 , C12N5/00 , C07H21/02
CPC分类号： C07K14/47 , C07K16/00 , C07K16/18 , C07K16/3007 , C07K16/3069 , C07K16/40 , C07K16/44 , C12N15/1037 , C12N15/1048 , C12N15/1062 , C12N15/1075 , C12N15/62 , C12N15/70 , C40B40/02 , C07K2317/34 , C07K2319/00 , C07K2319/20 , C07K2319/50 , C07K2319/61 , C07K2319/705 , C07K2319/72 , C07K2319/73 , C07K2319/735
摘要： A novel method for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARs) is disclosed. TSARs are concatenated heterofunctional proteins, polypeptides or peptides comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional proteins, polypeptides or peptides further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.
摘要翻译：公开了一种用于产生称为全合成亲和试剂（TSAR）的新型和/或改进的异功能结合融合蛋白的新方法。 TSAR是连接的异功能蛋白质，多肽或肽，其包含至少两个功能区：对配体具有亲和性的结合结构域和化学或生物活性的第二效应肽部分。在一个实施方案中，杂多功能蛋白质，多肽或肽还包含结合结构域和第二活性肽部分之间的接头肽部分。接头肽可以是敏感的或不易于通过酶促或化学方式切割。还公开了新型和/或改进的异型结合试剂以及用于多种体外和体内应用的方法。

8. 发明授权

US5498538A Totally synthetic affinity reagents 失效
标题翻译：全合成亲和试剂
公开(公告)号：US5498538A
公开(公告)日：1996-03-12
申请号：US176500
申请日：1993-12-30
申请人： Brian K. Kay , Dana M. Fowlkes
发明人： Brian K. Kay , Dana M. Fowlkes
IPC分类号： C07K14/47 , C07K16/00 , C07K16/18 , C07K16/30 , C07K16/40 , C07K16/44 , C12N15/10 , C12N15/62 , C12N15/70 , C12P21/04 , C40B40/02 , C12N15/09
CPC分类号： C07K14/47 , C07K16/00 , C07K16/18 , C07K16/3007 , C07K16/3069 , C07K16/40 , C07K16/44 , C12N15/1037 , C12N15/1048 , C12N15/1062 , C12N15/1075 , C12N15/62 , C12N15/70 , C40B40/02 , C07K2317/34 , C07K2319/00 , C07K2319/20 , C07K2319/50 , C07K2319/61 , C07K2319/705 , C07K2319/72 , C07K2319/73 , C07K2319/735
摘要： A novel method for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARs) is disclosed. TSARs are concatenated heterofunctional proteins, polypeptides or peptides comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional proteins, polypeptides or peptides further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.
摘要翻译：公开了一种用于产生称为全合成亲和试剂（TSAR）的新型和/或改进的异功能结合融合蛋白的新方法。 TSAR是连接的异功能蛋白质，多肽或肽，其包含至少两个功能区：对配体具有亲和性的结合结构域和化学或生物活性的第二效应肽部分。在一个实施方案中，杂多功能蛋白质，多肽或肽还包含结合结构域和第二活性肽部分之间的接头肽部分。接头肽可以是敏感的或不易于通过酶促或化学方式切割。还公开了新型和/或改进的异型结合试剂以及用于多种体外和体内应用的方法。

9. 发明授权

US06303574B1 Scr SH3 binding peptides and methods of isolating and using same 失效
标题翻译： SRC SH3结合肽及其分离和使用方法
公开(公告)号：US06303574B1
公开(公告)日：2001-10-16
申请号：US08278865
申请日：1994-07-22
申请人： Brian K. Kay , Andrew B. Sparks , Judith M. Thorn , Lawrence A. Quilliam , Channing J. Der
发明人： Brian K. Kay , Andrew B. Sparks , Judith M. Thorn , Lawrence A. Quilliam , Channing J. Der
IPC分类号： A61K3810
CPC分类号： C07K14/001 , A61K38/00 , A61K47/62 , G01N33/573 , G01N33/68
摘要： Peptides having general and specific binding affinities for the Src homology region 3 (SH3) domains of proteins are disclosed in the present invention. In particular, SH3 binding peptides have been isolated from three phage-displayed random peptide libraries which had been screened for isolates that bind to bacterial fusion proteins of SH3 domains and glutathione S-transferase (GST). Preferred peptides are disclosed having a core 7-mer sequence (preferably, a consensus motif) and two or more, preferably at least six, additional amino acid residues flanking the core sequence, for a total length of 9, preferably at least 13, amino acid residues and no more than about 45 amino acid residues. Such peptides manifest preferential binding affinities for certain SH3 domains. The preferred peptides exhibit specific binding affinities for the Src-family of proteins. In vitro and in vivo results are presented which demonstrate the biochemical activity of such peptides.
摘要翻译：在本发明中公开了对蛋白质的Src同源性区域3（SH3）结构域具有一般和特异性结合亲和力的肽。特别地，已经从已经筛选了与SH3结构域和谷胱甘肽S-转移酶（GST）的细菌融合蛋白结合的分离株的三个噬菌体展示的随机肽文库中分离出SH3结合肽。公开了优选的肽，其具有核心序列的核心7-聚体序列（优选共有基序）和两个或更多个，优选至少六个另外的核心序列侧翼的氨基酸残基，总长度为9，优选至少13个氨基酸残基和不超过约45个氨基酸残基。这些肽表现出对某些SH3结构域的优先结合亲和力。优选的肽显示出对Src家族蛋白质的特异性结合亲和力。提出体外和体内结果，证明了这些肽的生化活性。

10. 发明授权

US5852167A Totally synthetic affinity reagents 失效
公开(公告)号：US5852167A
公开(公告)日：1998-12-22
申请号：US471800
申请日：1995-06-06
申请人： Brian K. Kay , Nils B. Adey
发明人： Brian K. Kay , Nils B. Adey
IPC分类号： C07K14/47 , C07K16/00 , C07K16/18 , C07K16/30 , C07K16/40 , C07K16/44 , C12N15/10 , C12N15/62 , C12N15/70 , C12P21/04 , C40B40/02 , C07K14/00
CPC分类号： C07K14/47 , C07K16/00 , C07K16/18 , C07K16/3007 , C07K16/3069 , C07K16/40 , C07K16/44 , C12N15/1037 , C12N15/1048 , C12N15/1062 , C12N15/1075 , C12N15/62 , C12N15/70 , C40B40/02 , C07K2317/34 , C07K2319/00 , C07K2319/20 , C07K2319/50 , C07K2319/61 , C07K2319/705 , C07K2319/72 , C07K2319/73 , C07K2319/735
摘要： A novel method for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARs) is disclosed. TSARs are concatenated heterofunctional proteins, polypeptides or peptides comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional proteins, polypeptides or peptides further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.

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