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JP2014098724A Microscope device and fluorescence observation method using the same 有权
标题翻译：显微镜装置和使用其的荧光观察方法
公开(公告)号：JP2014098724A
公开(公告)日：2014-05-29
申请号：JP2008235795
申请日：2008-09-13
申请人： Japan Science & Technology Agency , 独立行政法人科学技術振興機構
发明人： IGARASHI YASUNOBU , OBARA TAKESHI , DEGUCHI YUKI , SUZUKI TAKESHI , HASHIMOTO KOICHI
IPC分类号： G02B21/00 , G01N21/64
CPC分类号： G02B21/0088 , G01N21/6458 , G02B21/0076 , G02B21/16 , G02B21/26 , G02B21/34
摘要： PROBLEM TO BE SOLVED: To provide a microscope device and fluorescence observation method controlling a position of an object to be observed such as a cell and controlling a fluorescent record.SOLUTION: A microscope device 1 comprises: a stage 3 on which an object to be observed 2 is placed; an irradiation light source 4 for irradiating the object to be observed 2; an exciting light source 5 for exciting fluorescence F in the object to be observed 2; a lens barrel part 15 forming a light path for light T by the irradiation light, the exciting light 5A, and the fluorescence F; an image information detection part 16 detecting image information by the light T generated in the object to be observed 2; fluorescence image information detection part 17 detecting fluorescence image information by the fluorescence F; and a control part 20 determining a fluorescence observation area of the object to be observed 2 on the basis of a movement model of the object to be observed 2 and the image information of the object to be observed 2 input from the image information detection part 16, and acquiring the image information of the object to be observed 2 input from the image information detection part 16 and the fluorescence image information input from the fluorescence image information detection part 17 at a prescribed interval within the fluorescence observation area.
摘要翻译：要解决的问题：提供一种控制诸如细胞等被观察物的位置的显微镜装置和荧光观察方法，控制荧光记录。显微镜装置1包括：载物台观察2放置; 用于照射被观察物体2的照射光源4; 用于激发待观察物体中的荧光F的激发光源5; 通过照射光，激发光5A和荧光F形成用于光T的光路的透镜镜筒部15; 图像信息检测部16，通过在待观察对象物2中产生的光T检测图像信息; 荧光图像信息检测部17，通过荧光F检测荧光图像信息; 以及控制部20，根据被观察物体2的移动模型和从图像信息检测部16输入的被观察物体2的图像信息，确定被观察物体2的荧光观察区域。并且从荧光观察区域中以规定的间隔获取从图像信息检测部16输入的被观察物体2的图像信息和从荧光图像信息检测部17输入的荧光图像信息。

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