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1. 发明授权

US09000146B2 Isolation of nucleic acids 有权
标题翻译：分离核酸
公开(公告)号：US09000146B2
公开(公告)日：2015-04-07
申请号：US13470251
申请日：2012-05-11
申请人： Janelle J. Bruinsma , Michael J. Domanico , Graham P. Lidgard , Hongzhi Zou , William G. Weisburg , Hemanth D. Shenoi , James P. Light, II
发明人： Janelle J. Bruinsma , Michael J. Domanico , Graham P. Lidgard , Hongzhi Zou , William G. Weisburg , Hemanth D. Shenoi , James P. Light, II
IPC分类号： C12Q1/68 , C12N15/10 , B03C1/30 , B04B3/00
CPC分类号： C12Q1/6806 , B01D33/15 , B01D33/155 , B01L3/00 , B01L3/5021 , B03C1/30 , B04B3/00 , C12N15/1006 , C12N15/1013 , C12N15/1017 , C12Q1/6886 , C12Q2600/158 , C12Q2600/16 , Y10T436/143333
摘要： Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting multiple DNA targets from a human stool sample with, for example, methods employing spin filtration techniques.
摘要翻译：本文提供了与分离核酸有关的技术。特别地，该技术涉及用于从例如使用旋转过滤技术的方法从人粪便样品中提取多个DNA靶标的方法和试剂盒。

2. 发明申请

US20120288868A1 ISOLATION OF NUCLEIC ACIDS 有权
标题翻译：分离核酸
公开(公告)号：US20120288868A1
公开(公告)日：2012-11-15
申请号：US13470251
申请日：2012-05-11
申请人： Janelle J. Bruinsma , Michael J. Domanico , Graham P. Lidgard , Hongzhi Zou , William G. Weisburg , Hemanth D. Shenoi , James P. Light, II
发明人： Janelle J. Bruinsma , Michael J. Domanico , Graham P. Lidgard , Hongzhi Zou , William G. Weisburg , Hemanth D. Shenoi , James P. Light, II
IPC分类号： C07H1/08 , G01N33/50 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6806 , B01D33/15 , B01D33/155 , B01L3/00 , B01L3/5021 , B03C1/30 , B04B3/00 , C12N15/1006 , C12N15/1013 , C12N15/1017 , C12Q1/6886 , C12Q2600/158 , C12Q2600/16 , Y10T436/143333
摘要： Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.
摘要翻译：本文提供了与分离核酸有关的技术。特别地，该技术涉及从有问题的样品如粪便中提取核酸的方法和试剂盒。

3. 发明授权

US08361720B2 Real time cleavage assay 有权
标题翻译：实时切割测定
公开(公告)号：US08361720B2
公开(公告)日：2013-01-29
申请号：US12946737
申请日：2010-11-15
申请人： Rebecca Oldham-Haltom , Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
发明人： Rebecca Oldham-Haltom , Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
IPC分类号： C12Q1/68 , C12M1/00
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/686 , C12Q1/6886 , C12Q2600/156 , C12Q2600/158 , C12Q2525/301 , C12Q2527/101 , C12Q2561/109 , C12Q2565/1015
摘要： A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.
摘要翻译：提供基于切割的实时PCR测定方法。一般而言，测定方法包括对包含a）PCR试剂的反应混合物进行核酸靶扩增，以及b）用于在扩增的核酸靶上进行皮瓣裂解测定的折叠裂解试剂至两组热循环条件。在所述第一组和第二组循环之间没有向所述反应中加入另外的试剂，并且在第二组循环的每个循环中，测量瓣探针的切割。

4. 发明授权

US08916344B2 Methylation assay 有权
标题翻译：甲基化测定
公开(公告)号：US08916344B2
公开(公告)日：2014-12-23
申请号：US12946745
申请日：2010-11-15
申请人： Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
发明人： Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6827 , C12Q2600/154 , C12Q2521/301 , C12Q2523/125 , C12Q2561/109
摘要： A method for detecting a methylated genomic locus is provided. In certain embodiments, the method comprises: a) treating a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an agent that modifies cytosine to uracil to produce a treated nucleic acid; b) amplifying a product from the treated nucleic acid using a first primer and a second primer, wherein the first primer hybridizes to a site in the locus that contain methylcytosines and the amplifying preferentially amplifies the methylated copies of the genomic locus, to produce an amplified sample; and c) detecting the presence of amplified methylated copies of the genomic locus in the amplified sample using a flap assay that employs an invasive oligonucleotide having a 3′ terminal G or C nucleotide that corresponds to a site of methylation in the genomic locus.
摘要翻译：提供了一种检测甲基化基因组座位的方法。在某些实施方案中，所述方法包括：a）将含有基因组座位的非甲基化和甲基化拷贝的核酸样品用修饰胞嘧啶至尿嘧啶的试剂处理以产生经处理的核酸; b）使用第一引物和第二引物扩增来自经处理的核酸的产物，其中第一引物与含有甲基胞嘧啶的位点的位点杂交，并且扩增优先扩增基因组基因座的甲基化拷贝，以产生扩增样品; 和c）使用采用具有对应于基因组基因座中的甲基化位点的3'末端G或C核苷酸的侵入性寡核苷酸的皮瓣测定来检测扩增样品中扩增的基因组座位的扩增的甲基化拷贝的存在。

5. 发明申请

US20130231256A1 MULTIPLEXED KRAS MUTATION DETECTION ASSAY 有权
标题翻译：多重KRAS突变检测测定
公开(公告)号：US20130231256A1
公开(公告)日：2013-09-05
申请号：US13594674
申请日：2012-08-24
申请人： Rebecca Oldham-Haltom , Hatim Allawi , Hongzhi Zou , Michael J. Domanico , Graham P. Lidgard
发明人： Rebecca Oldham-Haltom , Hatim Allawi , Hongzhi Zou , Michael J. Domanico , Graham P. Lidgard
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , C12Q2525/161 , C12Q2561/109 , C12Q2565/1015
摘要： Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.
摘要翻译：本文提供的试剂混合物包含多重扩增试剂和皮瓣测定试剂，用于在单次反应中检测含有34A，34C，34T，35A，35C，35T或38A点突变中任何一种的KRAS基因的突变体拷贝。还提供了使用试剂混合物和试剂盒进行其的方法。

6. 发明申请

US20120122106A1 Mutation Detection Assay 有权
标题翻译：突变检测测定
公开(公告)号：US20120122106A1
公开(公告)日：2012-05-17
申请号：US12946752
申请日：2010-11-15
申请人： Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
发明人： Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q1/6881 , C12Q2600/156 , C12Q2600/158 , C12Q2525/301 , C12Q2561/109 , C12Q2565/1015
摘要： A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3′ terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3′ terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.
摘要翻译：提供了样品分析的方法。在某些实施方案中，该方法包括：a）从包含基因组基因组的野生型拷贝和相对于所述基因组基因座的所述野生型拷贝的点突变的基因组基因座的突变拷贝的样品扩增产物，产生扩增样品，其中：i。使用第一引物和第二引物进行扩增; 和ii。第一引物包含与点突变碱基对的3'末端核苷酸，并且还包含与3'末端核苷酸的6个碱基内的单碱基错配除外的基因座中的序列完全互补的核苷酸序列; 和b）使用采用侵入性寡核苷酸的皮瓣测定来检测所述扩增样品中所述产物的存在。还提供了用于执行该方法的套件。

7. 发明申请

US20120122088A1 METHYLATION ASSAY 有权
标题翻译：甲基化测定
公开(公告)号：US20120122088A1
公开(公告)日：2012-05-17
申请号：US12946745
申请日：2010-11-15
申请人： Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
发明人： Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6827 , C12Q2600/154 , C12Q2521/301 , C12Q2523/125 , C12Q2561/109
摘要： A method for detecting a methylated genomic locus is provided. In certain embodiments, the method comprises: a) treating a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an agent that modifies cytosine to uracil to produce a treated nucleic acid; b) amplifying a product from the treated nucleic acid using a first primer and a second primer, wherein the first primer hybridizes to a site in the locus that contain methylcytosines and the amplifying preferentially amplifies the methylated copies of the genomic locus, to produce an amplified sample; and c) detecting the presence of amplified methylated copies of the genomic locus in the amplified sample using a flap assay that employs an invasive oligonucleotide having a 3′ terminal G or C nucleotide that corresponds to a site of methylation in the genomic locus.
摘要翻译：提供了一种检测甲基化基因组座位的方法。在某些实施方案中，所述方法包括：a）将含有基因组座位的非甲基化和甲基化拷贝的核酸样品用修饰胞嘧啶至尿嘧啶的试剂处理以产生经处理的核酸; b）使用第一引物和第二引物扩增来自经处理的核酸的产物，其中第一引物与含有甲基胞嘧啶的位点的位点杂交，并且扩增优先扩增基因组基因座的甲基化拷贝，以产生扩增的样品; 和c）使用采用具有对应于基因组基因座中的甲基化位点的3'末端G或C核苷酸的侵入性寡核苷酸的皮瓣测定来检测扩增样品中扩增的基因组座位的扩增的甲基化拷贝的存在。

8. 发明授权

US09127318B2 Multiplexed KRAS mutation detection assay 有权
标题翻译：多重KRAS突变检测法
公开(公告)号：US09127318B2
公开(公告)日：2015-09-08
申请号：US13594674
申请日：2012-08-24
申请人： Rebecca Oldham-Haltom , Hatim Allawi , Hongzhi Zou , Michael J. Domanico , Graham P. Lidgard
发明人： Rebecca Oldham-Haltom , Hatim Allawi , Hongzhi Zou , Michael J. Domanico , Graham P. Lidgard
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , C12Q2525/161 , C12Q2561/109 , C12Q2565/1015
摘要： Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.
摘要翻译：本文提供的试剂混合物包含多重扩增试剂和皮瓣测定试剂，用于在单次反应中检测含有34A，34C，34T，35A，35C，35T或38A点突变中任何一种的KRAS基因的突变体拷贝。还提供了使用试剂混合物和试剂盒进行其的方法。

9. 发明授权

US08715937B2 Mutation detection assay 有权
标题翻译：突变检测法
公开(公告)号：US08715937B2
公开(公告)日：2014-05-06
申请号：US12946752
申请日：2010-11-15
申请人： Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
发明人： Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q1/6881 , C12Q2600/156 , C12Q2600/158 , C12Q2525/301 , C12Q2561/109 , C12Q2565/1015
摘要： A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to the wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3′ terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3′ terminal nucleotide; and b) detecting the presence of the product in the amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.
摘要翻译：提供了样品分析的方法。在某些实施方案中，所述方法包括：a）从包含基因组基因组的野生型拷贝和相对于基因组基因座的野生型拷贝的点突变的基因组基因座的突变拷贝的样品扩增产物，产生扩增样品，其中：i。使用第一引物和第二引物进行扩增; 和ii。第一引物包含与点突变碱基对的3'末端核苷酸，并且还包含与3'末端核苷酸的6个碱基内的单碱基错配除外的基因座中的序列完全互补的核苷酸序列; 和b）使用采用侵入性寡核苷酸的皮瓣测定法检测扩增样品中产物的存在。还提供了用于执行该方法的套件。

10. 发明申请

US20120122105A1 REAL TIME CLEAVAGE ASSAY 有权
标题翻译：实时清除测试
公开(公告)号：US20120122105A1
公开(公告)日：2012-05-17
申请号：US12946737
申请日：2010-11-15
申请人： Rebecca Oldham-Haltom , Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
发明人： Rebecca Oldham-Haltom , Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/686 , C12Q1/6886 , C12Q2600/156 , C12Q2600/158 , C12Q2525/301 , C12Q2527/101 , C12Q2561/109 , C12Q2565/1015
摘要： A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.
摘要翻译：提供基于切割的实时PCR测定方法。一般而言，测定方法包括对包含a）PCR试剂的反应混合物进行核酸靶扩增，以及b）用于在扩增的核酸靶上进行皮瓣切割测定的折叠裂解试剂至两组热循环条件。在所述第一组和第二组循环之间没有向所述反应中加入另外的试剂，并且在第二组循环的每个循环中，测量瓣探针的切割。

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