专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20150044744A1 BIOLOGICAL ALKANE OXIDATION 审中-公开
标题翻译：生物碱性氧化
公开(公告)号：US20150044744A1
公开(公告)日：2015-02-12
申请号：US14363165
申请日：2012-11-22
申请人： Jan Christoph Pfeffer , Thomas Haas , Oliver Thum , Frank Erhardt , Eva Maria Wittmann , Christian Gehring , Sabine Hafkemeyer , Thomas Hueller
发明人： Jan Christoph Pfeffer , Thomas Haas , Oliver Thum , Frank Erhardt , Eva Maria Wittmann , Christian Gehring , Sabine Hafkemeyer , Thomas Hueller
IPC分类号： C12P7/52
CPC分类号： C12P7/52 , C12P7/02 , C12P7/16 , C12P7/24 , C12P7/40 , Y02E50/10
摘要： The invention relates to a method for oxidizing an alkane, comprising contacting the alkane with a type alkB oxidoreductase and using a type alkB oxidoreductase to prepare a mixture of oxidation products of an alkane, wherein the ratio of carboxylic acid to alcohol in the oxidation products is preferably greater than 1:1.
摘要翻译：本发明涉及一种氧化烷烃的方法，包括使烷烃与alkB氧化还原酶接触，并使用alkB型氧化还原酶制备烷烃氧化产物的混合物，其中氧化产物中羧酸与醇的比例为优选大于1:1。

2. 发明申请

US20170002342A1 GRANULES COMPRISING ISOMALTULOSE SYNTHASE 审中-公开
标题翻译：包含异构体合成酶的颗粒
公开(公告)号：US20170002342A1
公开(公告)日：2017-01-05
申请号：US15123514
申请日：2015-03-03
申请人： Frank Hellmers , Thomas Hueller , Thomas Dassinger , Oliver Thum
发明人： Frank Hellmers , Thomas Hueller , Thomas Dassinger , Oliver Thum
IPC分类号： C12N9/98 , C12N9/90 , C08L33/12
CPC分类号： C12N9/98 , C08L33/04 , C08L33/12 , C12N9/90 , C12N11/16 , C12Y504/99011 , C08F220/14 , C08F2220/1808
摘要： The invention provides granules comprising A) at least one enzyme selected from at least one of the groups selected from transferases of EC 2, hydrolases of EC 3, lyases of EC 4 and isomerases of EC 5, B) at least one polymer selected from C1-C10-alkyl acrylate polymer, C1-C10-alkyl methacrylate polymer and C1-C10-alkyl acrylate-C1-C10-alkyl methacrylate copolymer, preferably C1-C10-alkyl acrylate-C1-C10-alkyl methacrylate copolymer and C) at least one inorganic carrier material.
摘要翻译：本发明涉及含有A）至少一种选自由EC2转移酶，EC 3水解酶，EC 4裂解酶和EC 5异构酶组成的组中的至少一种的B）至少一种选自C1 C 10 - 烷基丙烯酸酯聚合物，甲基丙烯酸C1-C10烷基酯聚合物和丙烯酸C1-C10烷基酯 - 甲基丙烯酸C1-C10烷基酯共聚物，优选C1-C10烷基丙烯酸酯-C1-C10-烷基甲基丙烯酸酯共聚物，C）至少一种无机载体材料。

3. 发明申请

US20070264716A1 Methods and materials for the transformation of the yeast Pichia ciferrii 审中-公开
标题翻译：用于转化酵母毕赤酵母的方法和材料
公开(公告)号：US20070264716A1
公开(公告)日：2007-11-15
申请号：US11797745
申请日：2007-05-07
申请人： Daniel Boergel , Thomas Hueller , Steffen Schaffer
发明人： Daniel Boergel , Thomas Hueller , Steffen Schaffer
IPC分类号： C12N15/74
CPC分类号： C12N9/88 , C12N9/0008
摘要： The present invention discloses a transformation system useful for the genetic engineering of the industrial yeast Pichia ciferrii. It describes the isolation of auxotrophic mutants of this yeast and nucleotide sequences able to complement the auxotrophic requirements of these strains. The transformation system is based on mutant Pichia ciferrii cells auxotrophic for uracil or lysine, which are transformed with plasmids containing the URA3 or LYS2 genes as selection markers. Novel URA3 and LYS2 genes are also disclosed.
摘要翻译：本发明公开了一种可用于工业酵母毕赤酵母基因工程的转化系统。它描述了该酵母的营养缺陷型突变体的分离以及能够补充这些菌株的营养缺陷型要求的核苷酸序列。转化系统基于尿嘧啶或赖氨酸营养缺陷型突变型细胞培养细胞，其以含有URA3或LYS2基因的质粒转化为选择标记。还公开了新的URA3和LYS2基因。

4. 发明申请

US20100190219A1 PRODUCTION OF SPHINGOID BASES USING GENETICALLY ENGINEERED MICROBIAL STRAINS 有权
标题翻译：使用遗传工程微生物菌株生产刺槐根
公开(公告)号：US20100190219A1
公开(公告)日：2010-07-29
申请号：US12300397
申请日：2007-05-11
申请人： Steffen Schaffer , Marco Alexander Van Den Berg , Daniel Boergel , Thomas Hueller
发明人： Steffen Schaffer , Marco Alexander Van Den Berg , Daniel Boergel , Thomas Hueller
IPC分类号： C12P13/02 , C12N1/00 , C12N15/74 , C12N9/00 , C12N9/02 , C12N9/78
CPC分类号： C12N9/80 , A61K8/68 , A61K2800/86 , A61Q19/00 , C12N9/0071 , C12N9/1029
摘要： The present invention provides genetically engineered microbial strains, in particular genetically engineered yeast strains, that produce at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The present invention provides a method to obtain genetically engineered microbial strains producing at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The method comprises the steps of: a) increasing the expression of a polynucleotide encoding an enzyme having ceramide synthase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing a sphingoid base according to Formula I, and/or b) decreasing the expression of a polynucleotide encoding an enzyme having sphingolipid Δ8-desaturase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing phytosphingosine or dihydrosphingosine as sphingoid base, and isolating strains with the required productivity.
摘要翻译：本发明提供基因工程微生物菌株，特别是遗传工程酵母菌株，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。本发明提供了一种获得基因工程的微生物菌株的方法，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。该方法包括以下步骤：a）增加编码具有神经酰胺合成酶活性的酶的多核苷酸和/或具有神经酰胺酶活性的酶的表达，后者能够优先或甚至具体地水解含有类鞘氨醇碱的神经酰胺根据式I，和/或b）减少编码具有鞘脂和Dgr.8-去饱和酶活性的酶的多核苷酸的表达和/或具有神经酰胺酶活性的酶，后者能够优先或甚至具体地水解含有植物鞘氨醇的神经酰胺或二氢鞘氨醇作为鞘氨醇碱，并以所需的生产力分离菌株。

5. 发明授权

US08372595B2 Method for obtaining a microbial strain for production of sphingoid bases 有权
标题翻译：获得用于生产类鞘氨醇碱的微生物菌株的方法
公开(公告)号：US08372595B2
公开(公告)日：2013-02-12
申请号：US12300397
申请日：2007-05-11
申请人： Steffen Schaffer , Marco Alexander Van Den Berg , Daniel Boergel , Thomas Hueller
发明人： Steffen Schaffer , Marco Alexander Van Den Berg , Daniel Boergel , Thomas Hueller
IPC分类号： G01N33/53 , C12P7/18 , C12N1/00
CPC分类号： C12N9/80 , A61K8/68 , A61K2800/86 , A61Q19/00 , C12N9/0071 , C12N9/1029
摘要： The present invention provides genetically engineered microbial strains, in particular genetically engineered yeast strains, that produce at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The present invention provides a method to obtain genetically engineered microbial strains producing at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The method comprises the steps of: a) increasing the expression of a polynucleotide encoding an enzyme having ceramide synthase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing a sphingoid base according to Formula I, and/or b) decreasing the expression of a polynucleotide encoding an enzyme having sphingolipid Δ8-desaturase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing phytosphingosine or dihydrosphingosine as sphingoid base, and isolating strains with the required productivity.
摘要翻译：本发明提供基因工程微生物菌株，特别是遗传工程酵母菌株，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。本发明提供了一种获得基因工程的微生物菌株的方法，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。该方法包括以下步骤：a）增加编码具有神经酰胺合成酶活性的酶的多核苷酸和/或具有神经酰胺酶活性的酶的表达，后者能够优先或甚至具体地水解含有类鞘氨醇碱的神经酰胺根据式I，和/或b）减少编码具有鞘脂和Dgr.8-去饱和酶活性的酶的多核苷酸的表达和/或具有神经酰胺酶活性的酶，后者能够优先或甚至具体地水解含有植物鞘氨醇的神经酰胺或二氢鞘氨醇作为鞘氨醇碱，并以所需的生产力分离菌株。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式