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1. 发明申请

US20120128639A1 In Vitro Production Of Oligodendrocytes From Human Umbilical Cord Stem Cells 审中-公开
标题翻译：从人脐带干细胞体外产生少突胶质细胞
公开(公告)号：US20120128639A1
公开(公告)日：2012-05-24
申请号：US13322903
申请日：2010-05-28
申请人： James J. Hickman , Hedvika Davis
发明人： James J. Hickman , Hedvika Davis
IPC分类号： A61K35/30 , A61P25/00 , C12N5/079
CPC分类号： C12N5/0622 , C12N2500/90 , C12N2501/01 , C12N2501/11 , C12N2501/115 , C12N2501/135 , C12N2501/70 , C12N2501/81 , C12N2501/91 , C12N2506/025 , C12N2506/1369 , C12N2506/1392
摘要： The invention provides a method of producing oligodendrocytes by in vitro differentiation of human multi-potent progenitor cells (MLPCs). The method comprises culturing isolated MLPCs on a first surface in a serum-free defined culture medium; replacing the culture medium with serum-free culture medium supplemented with bFGF, EGF and PDGF-AA for approximately 24 hours; changing the cultured MLPCs into the supplemented serum-free culture medium further supplemented with differentiation factors norepinephrine, forskolin. and K252a; establishing a 3D environment by covering the culture with a second surface opposite and spaced apart from the first surface, so as to contain the MLPCs therebetween; and continuing to culture until a majority of the MLPCs have differentiated into oligodendrocytes. Additionally included is a method of treatment for a subject afflicted by a disease characterized by central or peripheral nervous system demyelination, the method comprising transplanting into the subject oligodendrocytes produced according to the method disclosed.
摘要翻译：本发明提供了通过体外分化人多能祖细胞（MLPC）产生少突胶质细胞的方法。该方法包括在无血清定义培养基的第一表面培养分离的MLPC; 用补充有bFGF，EGF和PDGF-AA的无血清培养基代替培养基约24小时; 将培养的MLPC变成补充的无血清培养基，进一步补充分化因子去甲肾上腺素，毛喉素。和K252a; 通过用与第一表面相对且间隔开的第二表面覆盖培养来建立3D环境，以便在其间容纳MLPC; 并继续培养，直到大多数MLPC已经分化为少突胶质细胞。另外包括治疗由以中枢神经系统或周围神经系统脱髓鞘为特征的疾病所折磨的受试者的方法，该方法包括将根据所公开的方法产生的少突胶质细胞移植到受试者中。

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