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    • 2. 发明授权
    • Simultaneous assay for determining drugs
    • 用于确定药物的同时测定
    • US07947465B2
    • 2011-05-24
    • US12187661
    • 2008-08-07
    • William F. LinkRenato B. del RosarioRandy V. SweetDavid L. King
    • William F. LinkRenato B. del RosarioRandy V. SweetDavid L. King
    • G01N33/00
    • G01N33/54326G01N33/94G01N2446/00Y10T436/101666Y10T436/108331Y10T436/25125
    • Bodily fluid is analyzed for the presence of drugs of a selected panel of drugs in a simultaneous assay in which sample of the fluid is incubated with additional amounts of all drugs of the panel, antibodies specific to each of the drugs of the panel, and microparticles, the microparticles being divided into subsets, one subset for each drug in the panel and each subset distinguishable from the others. The incubation is performed in a liquid medium in which competitive binding occurs, the drugs in the sample competing with those added to the assay medium for binding to the antibodies. In one procedure, the added drugs are pre-coupled to the microparticles while the antibodies are not, and the incubation is followed by further incubating the microparticles with labeled ligands that have affinity for the antibodies. In an alternative procedure, the added drugs are not coupled to the microparticles but are pre-labeled, while the antibodies are pre-coupled to the microparticles, and the assay proceeds without further incubation. In both alternatives, the microparticles are ultimately recovered from the assay medium and from any unbound species, and the recovered microparticles are analyzed by flow cytometry to obtain indications of the presence of the various drugs in the sample in an inverse manner by detection of the label, each drug differentiable from the others by the distinguishing features of the microparticles.
    • 在同时测定中分析体液中所选择的药物组的药物的存在,其中将流体样品与附加量的所有药物,面板每种药物特异的抗体和微粒 ,将微粒分为子集,面板中每种药物的一个子集,以及与其他子集可区分的每个子集。 孵育在其中发生竞争性结合的液体培养基中进行,样品中的药物与添加到测定培养基中的药物与抗体结合。 在一个程序中,将所添加的药物预先偶联到微粒,而不是抗体,然后孵育后,用对抗体具有亲和性的标记配体进一步孵育微粒。 在替代方法中,所添加的药物不与微粒偶联,而是预先标记,而抗体预偶联于微粒,并且测定在不进一步孵育的情况下进行。 在两种替代方案中,微粒最终从测定培养基和任何未结合物质中回收,并且通过流式细胞术分析回收的微粒,以通过检测标签以相反的方式获得样品中各种药物的存在的迹象 ,每种药物通过微粒的区别特征与其他药物不同。
    • 6. 发明授权
    • Simultaneous assay for determining drugs
    • 用于确定药物的同时测定
    • US08137987B2
    • 2012-03-20
    • US13086229
    • 2011-04-13
    • William F. LinkRenato B. del RosarioRandy V. SweetDavid L. King
    • William F. LinkRenato B. del RosarioRandy V. SweetDavid L. King
    • G01N33/52G01N33/543
    • G01N33/54326G01N33/94G01N2446/00Y10T436/101666Y10T436/108331Y10T436/25125
    • Bodily fluid is analyzed for the presence of drugs of a selected panel of drugs in a simultaneous assay in which sample of the fluid is incubated with additional amounts of all drugs of the panel, antibodies specific to each of the drugs of the panel, and microparticles, the microparticles being divided into subsets, one subset for each drug in the panel and each subset distinguishable from the others. The incubation is performed in a liquid medium in which competitive binding occurs, the drugs in the sample competing with those added to the assay medium for binding to the antibodies. In one procedure, the added drugs are pre-coupled to the microparticles while the antibodies are not, and the incubation is followed by further incubating the microparticles with labeled ligands that have affinity for the antibodies. In an alternative procedure, the added drugs are not coupled to the microparticles but are pre-labeled, while the antibodies are pre-coupled to the microparticles, and the assay proceeds without further incubation. In both alternatives, the microparticles are ultimately recovered from the assay medium and from any unbound species, and the recovered microparticles are analyzed by flow cytometry to obtain indications of the presence of the various drugs in the sample in an inverse manner by detection of the label, each drug differentiable from the others by the distinguishing features of the microparticles.
    • 在同时测定中分析体液中所选择的药物组的药物的存在,其中将流体样品与附加量的所有药物,面板每种药物特异的抗体和微粒 ,将微粒分为子集,面板中每种药物的一个子集,以及与其他子集可区分的每个子集。 孵育在其中发生竞争性结合的液体培养基中进行,样品中的药物与添加到测定培养基中的药物与抗体结合。 在一个程序中,将所添加的药物预先偶联到微粒,而不是抗体,然后孵育后,用对抗体具有亲和性的标记配体进一步孵育微粒。 在替代方法中,所添加的药物不与微粒偶联,而是预先标记,而抗体预偶联于微粒,并且测定在不进一步孵育的情况下进行。 在两种替代方案中,微粒最终从测定培养基和任何未结合物质中回收,并且通过流式细胞术分析回收的微粒,以通过检测标签以相反的方式获得样品中各种药物的存在的迹象 ,每种药物通过微粒的区别特征与其他药物不同。
    • 8. 发明授权
    • Multi-faceted, rotatable promotional display for vehicles
    • 多方位,可旋转的促销展示车
    • US07877913B1
    • 2011-02-01
    • US11958250
    • 2007-12-17
    • David L. King
    • David L. King
    • G09F21/04
    • G09F21/04G09F7/22G09F19/02G09F21/043
    • A multi-faceted, rotatable promotional display device for being retained relative to a vehicle for enabling the perception of multiple promotional messages from a single vantage point. A display casing with multiple display surfaces is rotatably retained relative to a base structure. Promotional messages are displayed relative to the display surfaces, and a drive arrangement rotates the display casing relative. The display casing can have a square, triangular, or other cross section. The base structure can have a cup portion with a raised peripheral ridge, and a shell can envelope the display casing. The display surfaces can be electronic display screens or light transmissive panels. A light source can be disposed within the display casing. A drive motor can be powered by a battery, a photovoltaic array, or a wind turbine. A clutch or a force sensor can prevent the application of excessive rotational force.
    • 一种多面,可旋转的促销显示装置,用于相对于车辆保持,以使得能够从单个有利位置感知多个促销消息。 具有多个显示表面的显示壳体相对于基座结构可旋转地保持。 相对于显示表面显示促销消息,并且驱动装置相对于显示器壳体旋转。 显示器壳体可以具有正方形,三角形或其它横截面。 基部结构可以具有凸起的周缘的杯部,壳可以包围显示壳。 显示表面可以是电子显示屏或透光面板。 光源可以设置在显示器壳体内。 驱动电机可由电池,光伏阵列或风力涡轮机供电。 离合器或力传感器可以防止施加过大的旋转力。
    • 9. 发明授权
    • Collapsible cart with shelf
    • 可折叠车与货架
    • US06457737B1
    • 2002-10-01
    • US09316413
    • 1999-05-21
    • David L. King
    • David L. King
    • B62B112
    • B62B3/006A47B31/04A47B43/00B62B2205/32
    • The invention provides a collapsible cart for transporting books, files, and other items comprising a cabinet spine, at least one collapsible shelf, at least one fastener, and a plurality of wheels. The cabinet spine has a first surface whereupon at least one collapsible shelf is pivotally mounted. The collapsible shelf is movable between a first position generally perpendicular with respect to the cabinet spine and a second position generally parallel with respect to the cabinet spine. A fastener is attached to the cabinet spine and is designed to be coupled with the collapsible shelf when in the second position. A plurality of wheels are mounted underneath the collapsible cart to allow the cart to be transported from one location to another location. In one embodiment of this invention, first and second doors are pivotally mounted to each side of the cabinet spine. The collapsible cart can be placed in a collapsed position by moving the first collapsible shelf to the second position, and the first and second doors to a closed position.
    • 本发明提供了一种用于运送书本,文件和其它物品的折叠式推车,包括柜脊,至少一个可折叠货架,至少一个紧固件和多个轮子。 柜脊具有第一表面,因此可枢转地安装至少一个可折叠货架。 可折叠货架可在相对于柜脊大致垂直的第一位置和大致平行于柜脊的第二位置之间移动。 紧固件连接到机架脊,并且被设计成当处于第二位置时与可折叠搁架联接。 多个轮子安装在可折叠推车的下方,以允许将手推车从一个位置运输到另一个位置。 在本发明的一个实施例中,第一和第二门枢转地安装在柜脊的每一侧。 可折叠车可以通过将第一可折叠搁板移动到第二位置并将第一和第二门移动到关闭位置而被放置在折叠位置。
    • 10. 发明授权
    • Apparatus for limiting chain wear
    • 用于限制链条磨损的装置
    • US6141892A
    • 2000-11-07
    • US296162
    • 1999-04-21
    • Dennis L. MooreDavid L. King
    • Dennis L. MooreDavid L. King
    • E02F3/14E02F5/06E02F5/10F16G13/06F16G13/18
    • E02F5/06E02F3/142E02F3/143F16G13/06F16G13/18
    • An apparatus for limiting sliding wear and kick-back wear in straight sidebar chains as well as offset sidebar chains includes a wear bar which is attached to the outer surface of the chain's sidebar. The wear bar includes a main body portion having a forward wear bar end in opposed relation to a rearward wear bar end. An inner wear bar surface is positioned adjacent the outer surface of the chain's sidebar and is separated from an outer wear bar surface by a distance at least as great as the distance by which the chain's pin heads extend beyond the sidebars. In one embodiment, the upper edge of the wear bar includes a pair of semi-circular openings which fit closely adjacent the protruding pin heads. In another embodiment, the openings in the wear bar fully encircle the pin heads. A recess formed in the lower wear bar edge is used to weld the wear bar to the chain's sidebar. The lower edge of the wear bar may also be hardened to limit wear to the wear bar itself.
    • 用于限制直边侧链条以及偏移侧边栏链条中的滑动磨损和反冲磨损的装置包括连接到链条侧边缘的外表面上的耐磨条。 磨损杆包括主体部分,其具有与向后磨损杆端部相对的前部磨损杆端部。 内部耐磨条表面位于链条侧边栏的外表面附近,并且与外部耐磨条表面分开至少与链条销头延伸超过侧边栏的距离一样大的距离。 在一个实施例中,磨损杆的上边缘包括一对紧密邻近突出销头配合的半圆形开口。 在另一个实施例中,磨损杆中的开口完全环绕销头。 在下部耐磨条边缘形成的凹陷用于将耐磨条焊接到链条的边栏上。 耐磨条的下边缘也可以被硬化以限制耐磨条本身的磨损。