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1. 发明申请

US20110124841A1 Polypeptides Modified by Protein Trans-Splicing Technology 审中-公开
标题翻译：通过蛋白质翻转技术修饰的多肽
公开(公告)号：US20110124841A1
公开(公告)日：2011-05-26
申请号：US12747653
申请日：2008-12-12
申请人： James F. Monthony , Paul Xiang-Qin Liu , Li Yang , Kaisong Zhou
发明人： James F. Monthony , Paul Xiang-Qin Liu , Li Yang , Kaisong Zhou
IPC分类号： C07K14/00 , C07K1/107 , C07K2/00 , C07H21/00 , C12N15/63 , C12N1/21 , C12P21/02
CPC分类号： C12N15/1027 , C07K1/1075 , C07K1/1077 , C07K14/00 , C12N15/63
摘要： The present invention relates to a method of preparing modified polypeptides, by linking a target polypeptide to a carrier molecule that is designed to bear one or more water-soluble polymer molecules, via protein trans-splicing. The polymer molecules can be attached to the carrier molecule either before or after ligation to the target polypeptide. Novel protein trans-splicing elements (known as “split inteins”) and trans-splicing partners are also provided.
摘要翻译：本发明涉及通过蛋白质转接将目标多肽与被设计为承载一种或多种水溶性聚合物分子的载体分子连接来制备修饰的多肽的方法。聚合物分子可以在与靶多肽连接之前或之后连接至载体分子。还提供了新的蛋白质转接元件（称为“分裂内含肽”）和转拼合子。

2. 发明授权

US08394604B2 Protein splicing using short terminal split inteins 有权
标题翻译：蛋白质拼接使用短端分裂内含肽
公开(公告)号：US08394604B2
公开(公告)日：2013-03-12
申请号：US12990402
申请日：2009-04-30
申请人： Paul Xiang-Qin Liu , Kaisong Zhou , Gerrit Volkmann
发明人： Paul Xiang-Qin Liu , Kaisong Zhou , Gerrit Volkmann
IPC分类号： C12P21/06
CPC分类号： C12P21/00 , C07K7/06 , C12P21/02 , C12P21/06
摘要： The invention provides methods of producing functional split inteins having small N-intein and/or small C-intein. Using these split inteins with their protein trans-splicing and cleavage activities, we provide new and more effective methods of manipulating proteins. They include site-specific addition of synthetic peptides at protein's terminal and internal locations, ligation of synthetic and/or expressed polypeptides, controllable cyclization of synthetic and/or expressed polypeptides, and controllable cleavages of recombinant proteins. These methods have numerous utilities including but not limited to protein fluorescence labeling, fixation on microchips, site-specific PEGylation, and linkage with pharmaceutical molecules.
摘要翻译：本发明提供了生产具有小N-内蛋白和/或小C-内含肽的功能性裂解内含肽的方法。使用这些分裂的内含肽及其蛋白质的翻译和切割活性，我们提供了更有效的操作蛋白质的方法。它们包括在蛋白质末端和内部位置处合成肽的位点特异性添加，合成和/或表达多肽的连接，合成和/或表达多肽的可控环化以及重组蛋白的可控裂解。这些方法具有许多实用性，包括但不限于蛋白质荧光标记，微芯片上的固定，位点特异性PEG化以及与药物分子的连接。

3. 发明申请

US20110172391A1 PROTEIN SPLICING USING SHORT TERMINAL SPLIT INTEINS 有权
标题翻译：蛋白质分离使用短端分离酶
公开(公告)号：US20110172391A1
公开(公告)日：2011-07-14
申请号：US12990402
申请日：2009-04-30
申请人： Paul Xiang-Qin Liu , Kaisong Zhou , Gerrit Volkmann
发明人： Paul Xiang-Qin Liu , Kaisong Zhou , Gerrit Volkmann
IPC分类号： C07K2/00
CPC分类号： C12P21/00 , C07K7/06 , C12P21/02 , C12P21/06
摘要： The invention provides methods of producing functional split inteins having small N-intein and/or small C-intein. Using these split inteins with their protein trans-splicing and cleavage activities, we provide new and more effective methods of manipulating proteins. They include site-specific addition of synthetic peptides at protein's terminal and internal locations, ligation of synthetic and/or expressed polypeptides, controllable cyclization of synthetic and/or expressed polypeptides, and controllable cleavages of recombinant proteins. These methods have numerous utilities including but not limited to protein fluorescence labeling, fixation on microchips, site-specific PEGylation, and linkage with pharmaceutical molecules.
摘要翻译：本发明提供了生产具有小N-内蛋白和/或小C-内含肽的功能性裂解内含肽的方法。使用这些分裂的内含肽及其蛋白质的翻译和切割活性，我们提供了更有效的操作蛋白质的方法。它们包括在蛋白质末端和内部位置处合成肽的位点特异性添加，合成和/或表达多肽的连接，合成和/或表达多肽的可控环化以及重组蛋白的可控裂解。这些方法具有许多实用性，包括但不限于蛋白质荧光标记，微芯片上的固定，位点特异性PEG化以及与药物分子的连接。

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