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    • 4. 发明申请
    • KRAS Primers and Probes
    • KRAS底漆和探针
    • US20130029336A1
    • 2013-01-31
    • US13640416
    • 2011-04-12
    • Craig Stephens
    • Craig Stephens
    • C12Q1/68C07H21/04
    • C12Q1/6886C12Q2600/106C12Q2600/156
    • The present invention provides oligonucleotide primers or probes for the detection of a mutation of the KRAS gene. The invention also provides a method for detecting a mutation in the KRAS gene using the oligonucleotide primers or probes disclosed therein. Furthermore, the present invention encompasses a method for predicting the sensitivity of a tumor in a patient to epidermal growth factor receptor-directed chemotherapy, comprising obtaining DNA from the tumor; and determining whether there is a mutation in codon 12 and/or a mutation in codon 13 in exon 2 of the KRAS gene in the DNA using a method utilizing at least one of the oligonucleotide primers and/or probes of the present invention.
    • 本发明提供了用于检测KRAS基因突变的寡核苷酸引物或探针。 本发明还提供了使用其中公开的寡核苷酸引物或探针检测KRAS基因突变的方法。 此外,本发明包括用于预测患者中肿瘤对表皮生长因子受体指导化疗的敏感性的方法,包括从肿瘤获得DNA; 并使用利用至少一种本发明的寡核苷酸引物和/或探针的方法,确定DNA中的KRAS基因的外显子2的密码子12的突变和/或突变的密码子13是否突变。